LOCUS       CE1CEA                   630 bp    DNA     linear   BCT 23-JUL-2016
DEFINITION  Plasmid ColE1 cea (3' end), imm and lys genes (complete cds).
ACCESSION   M12543
VERSION     M12543.1
KEYWORDS    cea gene; colicin; colicin E1; imm gene; immune response gene; kil
            gene; lys gene.
SOURCE      Plasmid ColE1
  ORGANISM  Plasmid ColE1
            other sequences; plasmids.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Waleh,N.S. and Johnson,P.H.
  TITLE     Structural and functional organization of the colicin E1 operon
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82 (24), 8389-8393 (1985)
   PUBMED   3936034
COMMENT     Original source text: Plasmid ColE1 (a colicin-producing derivative
            strain from E.coli strain JC411) DNA, clones pNP[4,6,12,14].
            The lys gene is also known as the kil gene.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Plasmid ColE1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:2443"
                     /plasmid="Plasmid ColE1"
     CDS             <1..35
                     /note="colicin E1"
                     /codon_start=3
                     /transl_table=11
                     /protein_id="AAA23065.1"
                     /translation="LNTINEVLGI"
     CDS             complement(32..373)
                     /note="immunity protein"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA23066.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIV
                     ISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLELFISIKNN"
     CDS             421..558
                     /note="lys (kil) protein"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA23067.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAV
                     Q"
BASE COUNT          201 a           96 c          113 g          220 t
ORIGIN      Unreported.
        1 aacttaatac tataaatgag gtgttaggga tttaattatt ctttattgat ataaaaagtt
       61 ctagcaatcc aaatgggatt gctaggacca aacaaagtag attatatagc ataaataggt
      121 ttaattttgc tacgggggcg ttatttaggt tttttcttct ttcgaaaaaa tctttcttta
      181 tgaagttaaa agctatgtat tcaatagcat attttgaata tggacataga atagtgctta
      241 tcactattgc atatagcatc ttatctgaca caaggaaata atacccttcg ctgttttttg
      301 ttataaggta tatatatata agtgtgcagt acaggccaaa taaaatattt tttatgtagt
      361 atcttaagct cataaattaa acctcgccat atattctttt cattttataa ggatcgagtt
      421 atgaggaaaa gattttttgt gggaatattc gcgataaacc tccttgttgg atgtcaggct
      481 aactatatac gtgatgttca gggagggacc atcgcaccat cctcctcttc taaactgacg
      541 gggatcgcgg ttcagtagaa aagattaaag gatcttcttg agatcctttt tttctgcgcg
      601 taatctgctg cttgcaaaca aaaaaaccac
//