LOCUS       SOYGLYAB                1786 bp    mRNA    linear   PLN 27-APR-1993
DEFINITION  Soybean (G.max) glycinin seed storage protein, subunit A3-B4, mRNA.
ACCESSION   M10962
VERSION     M10962.1
KEYWORDS    glycinin.
SOURCE      Glycine max (soybean)
  ORGANISM  Glycine max
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50
            kb inversion clade; NPAAA clade; indigoferoid/millettioid clade;
            Phaseoleae; Glycine; Glycine subgen. Soja.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Fukazawa,C., Momma,T., Hirano,H., Harada,K. and Udaka,K.
  TITLE     Glycinin A3B4 mRNA. Cloning and sequencing of double-stranded cDNA
            complementary to a soybean storage protein
  JOURNAL   J. Biol. Chem. 260 (10), 6234-6239 (1985)
   PUBMED   3838983
COMMENT     Original source text: Soybean (G.max) cotyledonary tissue, cDNA to
            mRNA, clone pGA3-B4-1425.
            Glycinin is synthesized as precursor polypeptides which undergo
            post-translational processing to form the nonrandom polypeptide
            pairs via disulfide bonds.
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Glycine max"
                     /mol_type="mRNA"
                     /db_xref="taxon:3847"
     mRNA            <1..1786
                     /product="A3B4 mRNA"
     CDS             47..1597
                     /note="glycinin"
                     /codon_start=1
                     /protein_id="AAA33964.1"
                     /translation="MGKPFFTLSLSSLCLLLLSSACFAITSSKFNECQLNNLNALEPD
                     HRVESEGGLIETWNSQHPELQCAGVTVSKRTLNRNGSHLPSYLPYPQMIIVVQGKGAI
                     GFAFPGCPETFEKPQQQSSRRGSRSQQQLQDSHQKIRHFNEGDVLVIPLGVPYWTYNT
                     GDEPVVAISPLDTSNFNNQLDQNPRVFYLAGNPDIEHPETMQQQQQQKSHGGRKQGQH
                     RQQEEEGGSVLSGFSKHFLAQSFNTNEDTAEKLRSPDDERKQIVTVEGGLSVISPKWQ
                     EQEDEDEDEDEEYGRTPSYPPRRPSHGKHEDDEDEDEEEDQPRPDHPPQRPSRPEQQE
                     PRGRGCQTRNGVEENICTMKLHENIARPSRADFYNPKAGRISTLNSLTLPALRQFGLS
                     AQYVVLYRNGIYSPDWNLNANSVTMTRGKGRVRVVNCQGNAVFDGELRRGQLLVVPQN
                     PAVAEQGGEQGLEYVVFKTHHNAVSSYIKDVFRVIPSEVLSNSYNLGQSQVRQLKYQG
                     NSGPLVNP"
     sig_peptide     47..118
                     /note="glycinin signal peptide"
     mat_peptide     119..1078
                     /product="glycinin subunit A3"
     mat_peptide     1079..1594
                     /product="glycinin subunit B4"
BASE COUNT          524 a          452 c          408 g          402 t
ORIGIN      162 bp upstream of MluI site.
        1 aattcaccaa ctccttcaaa cttattaaca ctttccttag ttcaatatgg ggaagccctt
       61 cttcactctc tctctttctt ccctttgctt gctactcttg tcgagtgcat gctttgctat
      121 tacctccagc aagttcaacg agtgccaact caacaacctc aacgcgttgg aacccgacca
      181 ccgcgttgag tccgaaggtg gtcttattga aacatggaac tctcaacacc ctgagctgca
      241 atgcgccggt gtcactgttt ccaaacgcac cctcaaccgc aacggctccc acttgccatc
      301 ttacttacct tatccccaaa tgatcattgt cgttcaaggg aagggagcaa ttggatttgc
      361 atttccggga tgtcccgaga cgtttgagaa gccacaacaa caatcaagca gaagaggctc
      421 aaggtcacag cagcaactac aagacagtca ccagaagatt cgtcacttca atgaaggaga
      481 cgtactagtg attcctcttg gtgttcctta ctggacctat aacactggcg atgaaccagt
      541 tgttgccatc agtcctcttg acacctccaa cttcaacaat cagcttgatc aaaaccccag
      601 agtattttac cttgctggga acccagatat agagcatccc gagaccatgc aacaacagca
      661 gcagcagaag agtcatggtg gacgcaagca ggggcaacac cgacagcagg aggaagaagg
      721 tggcagtgtg ctcagtggct tcagcaaaca tttcttagca caatccttca acaccaacga
      781 ggacacagct gagaaacttc ggtctccaga tgacgaaagg aagcagatcg tgacagtgga
      841 gggaggcctc agcgttatca gccccaagtg gcaagaacaa gaagacgaag acgaagacga
      901 agacgaagaa tatggacgga cgccctctta tcctccacga cgaccaagcc atggaaagca
      961 tgaagatgac gaggacgagg acgaagaaga agatcaacct cgtcctgatc accctccaca
     1021 gcgaccaagc aggcccgaac aacaagaacc acgtggaaga ggatgtcaga ctagaaatgg
     1081 ggttgaggaa aatatttgca ccatgaagct tcacgagaac attgctcgcc cttcacgtgc
     1141 tgacttctac aacccaaaag ctggtcgcat tagcaccctc aacagtctca ccctcccagc
     1201 cctccgccaa ttcggactca gtgcccaata tgttgtcctc tacaggaatg gaatttactc
     1261 tccagattgg aacttgaacg cgaacagtgt gacgatgact cgagggaaag gaagagttag
     1321 agtggtgaac tgccaaggga atgcagtgtt cgacggtgag ctaaggaggg gacaattgct
     1381 agtggtgccg cagaaccccg cggtggctga gcaaggggga gaacaaggat tggaatatgt
     1441 agtgttcaag acacaccaca acgccgtgag cagctacatt aaggatgtgt ttagggtaat
     1501 cccttcggag gttctttcca attcttacaa ccttggccag agtcaagtgc gtcagctcaa
     1561 gtatcaagga aactccggcc ctttggtcaa cccataaata acaacaagta tatatgaagg
     1621 tgtggtgagg ccatcttata tgaaataata ttaaaatata ttttgtgtaa taataaaact
     1681 atggcctatg tatttaccac cctccgaccc agcctatgtt aatatctgag tggcgttgta
     1741 cctttgaatc gccttaataa aatgtcagtc ttcaagtttt gtcttt
//