LOCUS LT727444 6213 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAMFhGNTIf1, complete sequence.
ACCESSION LT727444
VERSION LT727444.1
KEYWORDS .
SOURCE Mammalian expression vector pCAMFhGNTIf1
ORGANISM Mammalian expression vector pCAMFhGNTIf1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6213)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6213
/organism="Mammalian expression vector pCAMFhGNTIf1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3124."
/db_xref="taxon:1944901"
regulatory 82..173
/note="polyA"
/regulatory_class="terminator"
misc_feature 174..530
/note="3FR"
regulatory complement(608..690)
/note="lac"
/regulatory_class="promoter"
rep_origin 832..907
/note="SV40 ori"
regulatory 938..1012
/note="polyA"
/regulatory_class="terminator"
regulatory complement(938..1012)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(1124..1528)
/note="pMB1 ori"
CDS complement(2069..2929)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88471.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3065..3442
/note="hCMV"
/regulatory_class="promoter"
regulatory 3448..4696
/note="AG"
/regulatory_class="promoter"
misc_feature 3817..4696
/note="intron"
misc_feature 4707..4949
/note="ppMF"
CDS <4962..6176
/codon_start=1
/transl_table=11
/note="hGNTI"
/protein_id="SJL88472.1"
/translation="ALDGDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGR
VPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDC
GHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFR
FPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLY
RTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRK
GVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRT
NDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAP
PLTWEGYDPSWN"
BASE COUNT 1269 a 1696 c 1789 g 1459 t
ORIGIN
1 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
61 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
121 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
181 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
241 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
301 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
361 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
421 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
481 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
541 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
601 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga
661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
721 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
841 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
901 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
1021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
1081 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
1141 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
1201 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa
1261 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
1321 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
1381 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
1441 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
1501 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
1561 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
1621 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
1681 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
1741 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
1801 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
1861 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
1921 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
1981 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
2041 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
2101 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
2161 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
2221 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
2281 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
2341 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
2401 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
2461 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
2521 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
2581 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
2641 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
2701 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
2761 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
2821 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
2881 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
2941 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
3001 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
3061 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
3121 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
3181 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
3241 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
3301 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
3361 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
3421 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
3481 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
3541 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
3601 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
3661 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
3721 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
3781 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
3841 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
3901 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
3961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
4021 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
4081 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
4141 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
4201 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
4261 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
4321 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
4381 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
4441 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
4501 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
4561 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
4621 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
4681 tgtgaccggc ggctctagag tcgagaatga gatttccttc aatttttact gcagttttat
4741 tcgcagcatc ctccgcatta gctgctccag tcaacactac aacagaagat gaaacggcac
4801 aaattccggc tgaagctgtc atcggttact cagatttaga aggggatttc gatgttgctg
4861 ttttgccatt ttccaacagc acaaataacg ggttattgtt tataaatact actattgcca
4921 gcattgctgc taaagaagaa ggggtatctt tggataaaag ggctctcgat ggcgaccccg
4981 ccagcctcac ccgggaagtg attcgcctgg cccaagacgc cgaggtggag ctggagcggc
5041 agcgtgggct gctgcagcag atcggggatg ccctgtcgag ccagcggggg agggtgccca
5101 ccgcggcccc tcccgcccag ccgcgtgtgc ctgtgacccc cgcgccggcg gtgattccca
5161 tcctggtcat cgcctgtgac cgcagcactg ttcggcgctg cctggacaag ctgctgcatt
5221 atcggccctc ggctgagctc ttccccatca tcgttagcca ggactgcggg cacgaggaga
5281 cggcccaggc catcgcctcc tacggcagcg cggtcacgca catccggcag cccgacctga
5341 gcagcattgc ggtgccgccg gaccaccgca agttccaggg ctactacaag atcgcgcgcc
5401 actaccgctg ggcgctgggc caggtcttcc ggcagtttcg cttccccgcg gccgtggtgg
5461 tggaggatga cctggaggtg gccccggact tcttcgagta ctttcgggcc acctatccgc
5521 tgctgaaggc cgacccctcc ctgtggtgcg tctcggcctg gaatgacaac ggcaaggagc
5581 agatggtgga cgccagcagg cctgagctgc tctaccgcac cgactttttc cctggcctgg
5641 gctggctgct gttggccgag ctctgggctg agctggagcc caagtggcca aaggccttct
5701 gggacgactg gatgcggcgg ccggagcagc ggcaggggcg ggcctgcata cgccctgaga
5761 tctcaagaac gatgaccttt ggccgcaagg gtgtgagcca cgggcagttc tttgaccagc
5821 acctcaagtt tatcaagctg aaccagcagt ttgtgcactt cacccagctg gacctgtctt
5881 acctgcagcg ggaggcctat gaccgagatt tcctcgcccg cgtctacggt gctccccagc
5941 tgcaggtgga gaaagtgagg accaatgacc ggaaggagct gggggaggtg cgggtgcagt
6001 atacgggcag ggacagcttc aaggctttcg ccaaggctct gggtgtcatg gatgacctta
6061 agtcgggggt tccgagagct ggctaccggg gtattgtcac cttccagttc cggggccgcc
6121 gtgtccacct ggcgccccca ctgacgtggg agggctatga tcctagctgg aattagcacc
6181 tgtcgactgg agacctgcag gcatgcaagc tcg
//