LOCUS LT726811 5000 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAG-hUb-K48R, complete
sequence.
ACCESSION LT726811
VERSION LT726811.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAG-hUb-K48R
ORGANISM Mammalian expression vector pCAGGS-FLAG-hUb-K48R
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5000)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5000
/organism="Mammalian expression vector
pCAGGS-FLAG-hUb-K48R"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5902."
/db_xref="taxon:1944839"
misc_feature 13..36
/note="FLAG epitope"
CDS 46..276
/transl_table=11
/note="hUBB mutated mature sequence"
/protein_id="SJL86570.1"
/translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI
FAGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
misc_feature 187..189
/note="K48R"
regulatory 286..377
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 378..734
/note="3' flanking region of rabbit beta-globin"
regulatory complement(812..894)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1036..1111
/note="SV40 ori"
regulatory complement(1142..1216)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1142..1216
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1331..1732)
/note="pMB1 ori"
CDS complement(2273..3133)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86571.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3273..3650
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3656..4997
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4025..4949
/note="intron AG promoter"
BASE COUNT 1065 a 1327 c 1391 g 1217 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgcgatgca gatcttcgtg
61 aaaaccctga ccggcaagac catcactctg gaggtggagc ccagtgacac catcgaaaat
121 gtgaaggcca agatccaaga taaagaaggc atcccccccg accagcagag gctcatcttt
181 gcaggcaggc agctggaaga tggccgcact ctttctgact acaacatcca gaaagagtcg
241 accctgcacc tggtcctgcg tctcagaggt gggtaggtcg acgaagatct ttttccctct
301 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
361 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
421 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
481 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
541 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
601 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
661 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
721 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
781 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
841 gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
901 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
961 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
1021 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
1081 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
1141 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
1201 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
1261 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
1321 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
1381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata
1441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
1501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
1561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
1621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
1681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
1741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
1801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
1861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
1921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
1981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
2041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
2101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
2161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
2221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
2281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
2341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
2401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
2461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
2521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
2581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
2641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
2701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
2761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
2821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
2881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
2941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
3001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
3061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
3121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
3181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
3241 catttccccg aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata
3301 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
3361 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
3421 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
3481 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
3541 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
3601 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
3661 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
3721 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc
3781 cccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc
3841 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
3901 gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt
3961 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
4021 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
4081 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
4141 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
4201 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
4261 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
4321 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
4381 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
4441 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
4501 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
4561 ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
4621 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
4681 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
4741 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
4801 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
4861 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
4921 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
4981 ctcatcattt tggcaaagaa
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