LOCUS LC846638 15482 bp DNA circular ENV 27-DEC-2024 DEFINITION Uncultured bacterium KHI42 plasmid pKHI42 DNA, complete sequence. ACCESSION LC846638 VERSION LC846638.1 KEYWORDS ENV. SOURCE uncultured bacterium ORGANISM uncultured bacterium Bacteria; environmental samples. REFERENCE 1 (bases 1 to 15482) AUTHORS Hauschild,K., Smalla,K. and Shintani,M. TITLE Direct Submission JOURNAL Submitted (26-SEP-2024) Contact:Masaki Shintani Shizuoka University, Integrated Science and Technology; 3-5-1, Johoku, Chuo-ku, Hamamatsu, Shizuoka 4328561, Japan REFERENCE 2 AUTHORS Hauschild,K., Shintani,M., Suzuki,M., Wolters,B., Tokuda,M., Yamazaki,R., Masumoto,M., Moriuchi,R., Dohra,H., Bunk,B. and Smalla,K. TITLE The transferable resistome of biosolids -sequencing reveals carriage of clinically relevant antibiotic resistance genes JOURNAL Unpublished COMMENT Exogenous captured plasmid by biparental mating from the biosolids of wastewater treatment plant. Annotated by Prodigal v2.6.1 or DFAST and mannually by NCBI BLAST 2.2.18. ##Assembly-Data-START## Assembly Method :: SPAdes v. 3.14.0 Sequencing Technology :: MiSeq ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..15482 /collection_date="2018" /db_xref="taxon:77133" /environmental_sample /geo_loc_name="Germany" /isolate="KHI42" /isolation_source="river sediment" /lab_host="Escherichia coli" /mol_type="genomic DNA" /organism="uncultured bacterium" /plasmid="pKHI42" CDS 1..879 /codon_start=1 /gene="repA" /product="helicase RepA family protein" /protein_id="BFT68171.1" /transl_table=11 /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFFAL EAAMSIACSVAGGDLVGLAPAHTGRVVYLAGEDPQPALVRRVHAIGQHLNQSAREAIA ENLMLEPIMGKRLNVMDEAHLRRVIDYSAGARLIVLDTLSRIHSLDENSNGDMAHLVS VLEHIAATTGAAVLYLHHVNKGSARDGQTDQQQAARGASALIDNARWCGYVAKMTEQE AERLSDRGFDRAPIGNERRGLFVRFGVSKQNYDATPLDRWYQRHTGGVLLPVELQEIK RESGNKGGKGYDSAAY" CDS 860..1852 /codon_start=1 /gene="repC" /product="replication protein C, IncQ-type" /protein_id="BFT68172.1" /transl_table=11 /translation="MTQQRIKPAYAKFDPAHVFDGLFVPTKGKKRGRLYVPPRRFGDS EISFQGFEQLGADDQSILLAVSAQLGIDGLMIEAKPPGEIGQQLRLALDFDNDDGRPL KTKKTSLRSLLIDAGYDDPESGAALARIKESLNRMGNAQIREINHATGWDYRCNLLSS RFNHKTGEIFVAVNPRLTGAVFREQHVKVSLFERNELESEAAKLLHCWLCSNIRLGRS LGNGNGAHIDTLAPHVWGKAAWEEASTKVRSVRRGQLRDALDEISDRTRGLQGGMGWA IDQTSSGLVLVSRPKELPFLEGEHDMRPSEYHDLMSQPPMQEPDLFDPQDWTPD" CDS complement(1788..2444) /codon_start=1 /gene="tetR" /product="tetracycline resistance transcriptional repressor TetR" /protein_id="BFT68173.1" /transl_table=11 /translation="MIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHFRNKRALL DALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTRPGAPQM ETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEQQASEADAEERGEDQLTTSAS TMPARLQSAMKIVYEGGPDAAFERGLALIIGGLEQVRLSPASSPAGRTNLVLALAAGS " CDS 2574..3764 /codon_start=1 /gene="tet(A)" /product="tetracycline efflux MFS transporter Tet(A)" /protein_id="BFT68174.1" /transl_table=11 /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGAWSR ATST" CDS 4214..4402 /codon_start=1 /product="hypothetical protein" /protein_id="BFT68175.1" /transl_table=11 /translation="MSGLISYSELQQSAPLPVAIDYPAALALRQMALIQGKLPKYLLA PEVSALHHYVPDLHGFVA" CDS complement(4442..5374) /codon_start=1 /gene="tnp" /product="IS5-like element ISAav2 family transposase" /protein_id="BFT68176.1" /transl_table=11 /translation="MRETSWGRVKYRTTNWKAYNAALKARGELTIWLDRDMQWLARPT GKRGRCQKFSDAAIQFCLTIKCLFGQPLRQTLGLVESLLGMAGLHWPVPDYSTVCRRQ KSLDVQVRYRASDKGLHMLADSTGIKFLGEGEWKTKKHGAERRRQWRKVHLGIDAQTL QIRAIAVTTNEVGDSPMAADLLGQIPSNEEIASFTGDGAYDTQDVHKACYLRGAIPII PPRKGAKLRKGLAFAHRNEAVKACRQLGRAIWKRWSGYHRRSLVETKMNCFKRLGEKV MARTFERQVTELHIRASILNQFTALGTPQTVAAA" CDS complement(5528..6412) /codon_start=1 /gene="mph(E)" /product="Mph(E) family macrolide 2'-phosphotransferase" /protein_id="BFT68177.1" /transl_table=11 /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWL LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL ETQNESLIVGAKAQLGVI" CDS complement(6468..7943) /codon_start=1 /gene="msr(E)" /product="ABC-F type ribosomal protection protein Msr(E)" /protein_id="BFT68178.1" /transl_table=11 /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC" CDS complement(8802..9566) /codon_start=1 /product="OfxX fusion product" /protein_id="BFT68179.1" /transl_table=11 /translation="MEVAMRIKIKGEITAERLAEALHAAAEKYEAVRPGHKVYGANLY LTAFDADGLPFDLVDHRGEPLSITIEAKSGELVKPALTAEGEAHRQKAKEEARRQAEE AEAEAQRRHRQTLDEYEQERQKRRKKEAEARKQFEDANAITAELLKTMPERFIDELNK TVQGVWDDLKPTETQGKKKGQPKALPVFSIHADGLVLSVETWKNPRRVLNPLCTLQHG EIAPFWMHEAWLEAMRRIVDLLDTLTAAPAEALESQ" CDS complement(9551..10189) /codon_start=1 /gene="mobE" /product="hypothetical protein" /protein_id="BFT68180.1" /transl_table=11 /translation="MSDLDDSFAKLLGRQPSDAERQSLYRVRDALGLKNNDALWLVLM ALQHYQGQYEKFPQAIAQAAKDTLVNFKVTADATVKASAEAAKADLAQAVAAAAQEVA HNTSAKQMWQWAAGCIAVAFLCVGLFGWYMHSSGKDSGYQAGYGAGYTEAKDEKAAAA WANTPEGRLAYRFAQTGSLASLAKCDRPGWYVEKGVCYVKPASDGTYGWRLP" CDS complement(10182..10865) /codon_start=1 /gene="mobD" /product="protein mobD" /protein_id="BFT68181.1" /transl_table=11 /translation="MAKSIYLIGGSKGGVGKSLVTMATVDYLQERGESVLLIESDTSN PDVWKAYKESTETELINLDEADGWIQLVNLCDSKPDSVVVINTAARNNKGVSAYGETL NSTLEELKRKLVTLWVINRQRDSLELLKEYMDAIPNADVHVVRNGHFGEEKKFELYNG SKLRTAVEERGGKSVTFPDMADRVSDDIYSKRMSIAVALKELPIGNRAELTRWRNEAK KVLEAVVHE" CDS complement(10878..11234) /codon_start=1 /gene="mobC" /product="hypothetical protein" /protein_id="BFT68182.1" /transl_table=11 /translation="MKYTTEQLEAIASKLREMPPVEKKKQEHSKQEAVRVLSKEIAAL QKRGYTLDQISETLRGEGLSIATPTLKSYLQRAKPAKKAPVQAPGDTPPPRPADKKPA DTSKATFTPKPDSDDI" CDS 11340..11855 /codon_start=1 /gene="mobB" /product="hypothetical protein" /protein_id="BFT68183.1" /transl_table=11 /translation="MVEQYRMRIVIHMLRMFLPIPACPFGASLLTVWLILQGASPLTP DSAALSLLTVQDTPRPRPRREGDMMPFETQGPEPLDAVINVRLTAAEKARLKEDADLA GLSMSELVRRRYFGRPIIANADAVMLKELRRIGGLLKHIHNESGGVYSKETAGALVAL KDYFRKLNDDR" CDS 11845..14517 /codon_start=1 /gene="mobA" /product="DNA-primase RepB domain-containing protein relaxase" /protein_id="BFT68184.1" /transl_table=11 /translation="MIVKKVPNPKKSASKAQRIGQLTSYVRSPESESPQEKCLYAGAR GFMMDDPKSQTAEMIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELG WKDHQAIYGLHSDTDNIHLHIVINRVHPETLKIVEKNRGFDIELAHKAIARIEHAQGW QREQNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAIEDGAAIIK QAQTWEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQP APQRQQVAPREPEPIKPDVPGWKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQ KARRDELMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDL EQWQRMQKSPELAEQWRHRASEPQRIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEE AGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVMGNDEYKAMCGKLAA EHGFKITNPELQESIQQERQRIQQERVQAMKSEQLKQFERYAEAVGAERYRVTSIKMR EDGSKQTFILDKKDGITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDD MNREKLERLIRDGYQPAAVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGD PKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLLKAERRECIKALALSSQIDAEYQR QAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRDVLKRQRGGEVDLSRLDSMIAV RMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYAYSAAGDRQAAELGKY RQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSL" CDS 14584..14808 /codon_start=1 /gene="mazE" /product="antitoxin MazE family protein" /protein_id="BFT68185.1" /transl_table=11 /translation="MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQ CLLVAQADSADTAMQQFMDEALADVDGWTE" CDS 14805..15131 /codon_start=1 /gene="mazF" /product="type II toxin-antitoxin system PemK/MazF family toxin" /protein_id="BFT68186.1" /transl_table=11 /translation="MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTL NAAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSKVGPAFGRIDADALLEVERCLAV FLGIAK" CDS 15128..15454 /codon_start=1 /product="hypothetical protein" /protein_id="BFT68187.1" /transl_table=11 /translation="MKTIYTIPAPDSLGAMIEVYGEPENAWYEWRIIDGGRTVRDTGT EGHSAFQGRQYGQAEIALRDALMFASGLPIDGDTPTDAASLEAGYAAKAKAEAARQGA GTPGSA" BASE COUNT 3382 a 4404 c 4184 g 3512 t ORIGIN 1 atggctttag acattatggc ggccttcacc aatgagccgc cagaacttga tttcatctgg 61 cccggattct tggccggaac cgtgggcgca cttgttgcac ctggcgcaac tggcaagagc 121 ttttttgctc ttgaagcggc catgtcaatc gcttgcagtg tggcaggcgg cgaccttgta 181 ggactggccc cggcgcacac cgggcgcgtg gtctatctcg ctggcgaaga tccacagccc 241 gcccttgtgc ggcgtgtcca cgccatcggc cagcacctca accagtcggc ccgcgaagcc 301 atcgctgaga acctgatgct tgagccgatc atgggcaagc ggttgaacgt gatggacgag 361 gcgcacttgc gccgcgtcat cgactacagc gcaggggcgc ggctgattgt gctggacaca 421 ttgagccgca ttcacagcct cgacgagaac agcaacggcg acatggccca ccttgtttcc 481 gtgttggaac acatcgcggc aaccaccggg gcggctgtcc tgtacctgca ccacgtcaac 541 aagggcagcg cccgcgacgg ccagaccgac cagcagcagg cagcgcgggg cgcgtctgcc 601 ctgatcgaca acgccagatg gtgcggctat gtcgccaaga tgacggagca ggaagccgag 661 cgcctgagcg accggggctt tgatcgtgcg cccatcggca acgagcggcg cggccttttt 721 gtccgctttg gcgtgagtaa gcagaactac gacgcgaccc cgctagaccg ctggtatcag 781 cgacacaccg gcggggtgtt gctgccggta gagctacagg agatcaagcg tgagagcggc 841 aacaaggggg gcaagggcta tgactcagca gcgtattaaa ccggcctatg ccaaattcga 901 tcccgctcat gtgtttgacg ggcttttcgt ccccacgaag gggaagaagc gcgggcgact 961 gtatgtgccg ccgcgccgct tcggcgatag tgaaatcagc tttcaagggt tcgagcagct 1021 tggagctgat gaccaatcaa tcttgcttgc agtaagcgcc cagcttggca ttgacggctt 1081 gatgatcgag gcaaaaccac ccggcgaaat aggccagcaa ttgcgccttg ctttggactt 1141 tgacaacgat gatggaaggc cgctgaaaac caagaaaacc agtttgcgca gcctgctgat 1201 tgatgctgga tatgacgacc cagaaagcgg ggcggcactt gccagaatca aggaaagcct 1261 gaaccgaatg ggtaacgcgc agatacggga aatcaatcac gctaccggct gggattaccg 1321 ctgcaatctc ctttcgagtc ggttcaatca caagactggg gaaatctttg ttgccgtcaa 1381 tccgcgcctg accggggcgg tttttcgaga gcaacacgtc aaggtttcgc ttttcgagcg 1441 caacgagctg gagagtgaag ccgccaagct gctgcactgt tggctgtgct caaacatccg 1501 gcttggccgt tcgcttggca atggcaacgg ggcgcacatc gacacgctag cgccccacgt 1561 atggggcaag gcggcgtggg aagaagcatc aacgaaggtt cgatcagtca ggcggggtca 1621 actacgcgat gctttagacg aaatctcaga ccgcacacgc ggactgcaag gcggtatggg 1681 atgggccatc gaccagacca gcagcgggct tgtgctggta agtcgtccca aggaactgcc 1741 gttccttgag ggtgagcacg acatgcgccc cagcgaatac cacgacctca tgagccagcc 1801 gccaatgcaa gaaccagatt tgttcgaccc gcaggactgg acgccggact gagccgcacc 1861 tgttcaagac cgccgatgat gagagccagg cctcgctcga atgccgcgtc cggaccgcct 1921 tcgtagacga ttttcatcgc gctctgtagg cgcgccggca tcgtagacgc tgaggtggtc 1981 aactgatctt cgccccgctc ctcggcgtct gcctcgctag cttgctgctc aagaacagcg 2041 ccgacggtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc 2101 gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc 2161 gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg 2221 gcattcccga tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga 2281 ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc 2341 cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg 2401 tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt cggctgcaac 2461 tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga 2521 taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacgtgaaac 2581 ccaacagacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc 2641 tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg 2701 cccactatgg cattctgctg gcgctgtatg cgttgatgca atttgcctgc gcacctgtgc 2761 tgggcgcgct gtcggatcgt ttcgggcggc ggccggtctt gctcgtctcg ctggccggcg 2821 ctgctgtcga ctacgccatc atggcgacgg cgcctttcct ttgggttctc tatatcgggc 2881 ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat attgccgata 2941 tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt ttcgggttcg 3001 ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc cacgctccgt 3061 tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc cttttgccgg 3121 agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg ctcgcttcgt 3181 tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc ttcatcatgc 3241 aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat cgctttcact 3301 gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat tcactcgccc 3361 aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca ctcatgctcg 3421 gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg ggatggatgg 3481 cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg ctgcaagcaa 3541 tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca ctggcggcgc 3601 tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat gcggcttcta 3661 taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg ctctgcctgc 3721 cggcgctgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca 3781 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt 3841 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg 3901 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct 3961 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 4021 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 4081 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cccctacgtg 4141 ctgctgaagt tgcccgcaac agagagtgga accaaccggt gataccacga tactatgact 4201 gagagtcaac gccatgagcg gcctcatttc ttattctgag ttacaacagt ccgcaccgct 4261 gccggtagct attgactatc cggctgcact agccctgcgt cagatggctc tgatccaagg 4321 caaactgcca aaatatctgc tggcaccgga agtcagcgcc ctgcaccatt atgttccgga 4381 tctgcatggc tttgttgcat aaatcggcca taggccaagg catccccaat cccaaataga 4441 tttacgcagc agcgactgtc tggggcgtgc ccagagcggt aaattgattg aggattgaag 4501 ctcggatgtg cagctcggtc acctggcgct caaacgtcct ggccatcacc ttctcgccca 4561 ggcgcttgaa gcaattcatc ttggtctcca ccagtgacct tcggtggtag ccgctccagc 4621 gtttccagat agcccgcccc aactgcctac acgccttgac cgcttcattg cggtgagcaa 4681 atgccagccc tttgcgcagc ttcgctcctt tgcgcggcgg gatgattgga atggctcccc 4741 gcaggtagca cgccttatgt acatcctgcg tgtcgtacgc cccatcaccg gtaaagctag 4801 ctatctcttc attgcttgga atttgaccta gcaggtctgc tgccatgggc gagtcgccca 4861 cttcattggt tgttactgcg atggcccgaa tctgcagtgt ctgggcatca atacccagat 4921 gcaccttgcg ccactggcgg cggcgctcgg caccgtgctt tttggttttc cactcacctt 4981 cgcccaagaa cttgatgccc gtggagtcgg ccagcatgtg caagcctttg tcacttgcac 5041 ggtagcgcac ctgcacatcc aagctctttt gcctgcgaca caccgtgctg tagtcgggca 5101 ctggccaatg caggcctgcc atacccaata gcgactcaac caggcccagt gtctggcgca 5161 agggctggcc aaacaagcac ttgatggtca ggcaaaactg gatggccgca tctgagaact 5221 tctggcaacg accacgcttg cccgtgggcc gagctagcca ctgcatatcc ctatcgagcc 5281 aaatggtcag ctcacctcgc gccttcaacg ctgcgttgta agccttccag ttcgttgtgc 5341 ggtacttaac ccgaccccag ctcgtctctc tcatctcccg agcctacctc agctgcgacc 5401 ggcatttgtg caacaacgcc gttagaaatg ccccttgatc gaggcatttt ctataaagaa 5461 tactaaaaac taattaaagc caaccaagtt tcaaaacaag gcagttctca gcaatcatat 5521 ttttaaatta tataactccc aactgagctt ttgctccaac gataaggctt tcattttgag 5581 tttctaaggc aaataaacca tacatcaaag gagaggccgc tgctctttct aaagtctgtt 5641 catatagttt attccaaact ttacccccta gtttttcata ctcgatgatt agagttttga 5701 ggctttcttc tccaaacaaa gttacatgcc cagcaaaatc aatcgctggg tcatctatat 5761 gggctgttga ccaatcaata acgcctgaaa cagctccatc ctttgaagct agtacatgcc 5821 cagcatataa atcgccatgt ataaattggg tgaaatctgc ccatagaaca tcattatcca 5881 accattttct gtagcgggtt tccaattgct cacttatacc aatttcagat tttactaact 5941 gcaaattgtt tgctatttca ggtcttaaat ctgaaggttt cataattttc aaatcatttt 6001 cccgaacttc tttttcagga atactatgga tttcaaataa ggtttttgcc aaagatgtta 6061 tgtatttcgg gctatctttg tccatattcc aaattatttc ataggtttca gcatccaaat 6121 ttaaaacagg attatcttta agtatgggat aagccactaa ttctgtagat gaaattctcc 6181 aatcaggaac ctctacagaa agatgttttt ttaccaattc taaaatgcgt ttttctttct 6241 tgatttgttc cctcatgcca tcacgacgag gaatacgcag caaccattgt tgcccctttg 6301 tatcaagagc aaaaacgacc ttaaaatcaa tgcccatttc attgaaattc attttgtccg 6361 taagcaacaa gccgtgtgct tcagcaagtg attgaatatc ttgaattgtc atttttaatt 6421 tcctttaaag agttcaataa ttaatgttcg gattagattg gctatcatta acaatctctc 6481 tcaaaagtct tgatgatttt gtggtctttg atctcgtaga taatgtcagc aatattatcg 6541 accaattgct tgtcatgaga tacgaagata atagttcctg cataggactt catcattgtt 6601 tctaatgcgg caatactttt taggtcaaga tagtttcctg gttcatccat aagcaaaata 6661 ttatattttc ctaaaagcat tttggataaa agcagtttga tgatttcacc tcccgataag 6721 tcggataagt ttttttgaat atcattcgct ccgatcccca ttgaagccaa tactgcacga 6781 atttccgcaa ctgtgtactc gcactcttcc tgcataaagg agagcacaga tttatgcgtg 6841 ttaaatttat atcctgtttg tgtaaagtag ccaatttcag cttttggaga tatggttaat 6901 ccatcagcac gttctgatat catttttaac aaggacgttt tccctgttcc attcgatcca 6961 gttatagcga ctttagcgcc aagcggtatt ataaagttag cgtcatcaaa gatagtacgg 7021 ctaccaaatt ttaagctcag accatctgcc gtaatcggga acttattgtg cagttctagg 7081 gctgaacttt gacgaaaacg aatagaacgc aaatgctctg gtgcttgaat atcttctaat 7141 gcagccaaac gcttttccat actcttagct gcctgataca gttttctttg cttggtgcca 7201 gtcatttttg catgcccaag tcgtccagca ctttcggtag agtttttgga tttttctcct 7261 tttttcttat tgtctaatcg attagcttgc tggcgttttt cttgcacagc agattctaat 7321 cgctcccgtt ccttcatcat cagctcatat tctacggctt ggtgttgtcg ctcttcttct 7381 ttttgacgca agtaatccga gtaaccaccc caatattccg taattttacc gtcttttaac 7441 tcccatatct tgtctacaac catatcaaga aaatatcggt catgactgat aacaagtaat 7501 gctccatcaa atgctttaag ttgaccaata agtagatcta ttccattgag atcaaggtgg 7561 ctggttggtt catccgctag aatgccatgt acttgttggg aaaatgcggc agcaattttt 7621 gcacgagttt cctctccgcc actcattgtg tcgttttgta cattggaaac accaaggcga 7681 gataacattg cccggtcttc gaccgtttct atttcgattc cgcccagttg gctgatatgt 7741 gcaaaatcac caaaacgctg taatgtcgct tcggctaaaa caatttcgcc attaagtact 7801 ttgagtaaac tactctttcc tgctccgtta tcacccacaa gaccaatacg gtcataagag 7861 tgaatttcca attcatcaat atccaaaaca tcacgcccag cataatccaa gcgtatgttt 7921 ctcgctttaa taattaaact catttttatt tactcctgtt tagctcttga aattttttat 7981 gcagcaaaca ggatttaggt gaaaacaaaa gctagcatca cagtgtcctc ccaaaaaaaa 8041 agctattcat ccacagggtg gacaaatagc tagtcaatta agttataact ggaaactatg 8101 cactaaaagc atacttataa attaagcata cttttacttt atatatccgc atatattctt 8161 aaagacacaa caaaagccca ccattataaa atagtgtcac tatgcaaata gttgtgtctt 8221 aacgaatgcg gatagaatgc ataaacttac ctaaaaaata aaattcagtt ttatgataac 8281 tttactgtta aaagaagtct agacaacctt gttttatgct tggatataag gcttatttta 8341 aaataatagt agtgaagatt ttctaaaatt aacataatac accttaagtg tagtgtgttt 8401 gtctatcaat aagagattta tctaatttaa ttcaagcccc cggcgcaggt ggtaaccacc 8461 cccggcgcag gtggtaacca cccccggcgc aggtggtaac cacccccggc gcaggtggta 8521 accacccccg gcgcaggtgg taaccacccc cggcgcaggt ggtaacctga caagccacaa 8581 aaacgcccgc aaacccttgt cagtactgag ttttcgcgtt tttccaaaat tccggtctaa 8641 gaagttctaa agaacttcta ccatgcgcgg ctttcagccg ccgctttagg cgtcgtctga 8701 agccttgcct tcgggcttcg ccctgcgcgg gctaaagccc ttgcagacac acccccgccc 8761 ccgccccgcc gctaccccct tccttgcagg cgaaaacgcc tctactggct ttctagggcc 8821 tctgccgggg ctgcggtgag cgtatccagc agatcgacta tccgcctcat ggcttctaac 8881 cacgcttcgt gcatccagaa tggggctatt tcgccgtgct ggagtgtgca aagcggattg 8941 agaacccggc gcgggttctt ccatgtctcg acggaaagca ccagcccgtc cgcatggatg 9001 gaaaagactg gtagcgcttt cggctggccc ttcttcttgc cttgcgtttc ggttggcttg 9061 aggtcgtccc aaacgccttg cacggttttg ttcagttcgt cgatgaagcg ttccggcatg 9121 gttttcaaga gttcggcagt gattgcgttc gcgtcctcaa attgctttct tgcttcggct 9181 tcctttttcc gtcgtttttg ccgctcctgt tcgtattcgt ctaaggtttg cctgtgtctg 9241 cgctgtgctt cagcttccgc ttcttcagct tgccttcgtg cttcctcttt ggctttctgt 9301 cggtgtgctt caccctctgc tgtgagtgct ggttttacca gttcgccaga cttggcctcg 9361 atggtgatcg aaagcggttc gccgcgatgg tctaccagat cgaacggcag accatcagca 9421 tcgaaggcgg ttaggtagag attggcccca tagaccttgt gccccggcct cacagcttcg 9481 tatttttctg ctgctgcgtg cagtgcttcc gctaggcgtt ctgccgtgat ttctcctttg 9541 atcttgattc tcatggcaac ctccatccgt aggtgccatc cgatgcgggc ttgacgtagc 9601 acacgccttt ttcgacgtac caccccggcc tatcgcactt cgccaagctg gcgaggcttc 9661 ccgtttgcgc gaagcggtag gccaagcgcc cctctggcgt gttggcccat gccgccgccg 9721 ctttctcgtc cttggcttcg gtgtatcccg cgccgtatcc ggcctgatag cccgaatcct 9781 tgccgctgct gtgcatgtac cagccgaaca ggccgacgca caggaaggcc accgcgatgc 9841 agcccgccgc ccactgccac atctgctttg ccgatgtgtt gtgcgcgacc tcttgcgctg 9901 ctgctgctac cgcttgcgcc aaatccgcct tggctgcttc cgctgatgct ttcacggttg 9961 catccgccgt taccttgaaa ttgaccaagg tatctttggc cgcttgtgct atcgcttgcg 10021 ggaacttctc atactggcct tggtagtgtt gcaaggccat gagcaccagc cacagcgcat 10081 cattgttttt caggccgagc gcatcccgaa cccggtaaag actttgccgc tccgcgtcac 10141 taggttgcct gccgagcagc tttgcaaagc tgtcgtctag gtcactcatg gactacggcc 10201 tccaatacct ttttggcctc gttgcgccac cgcgtaagct ccgcacggtt gccaatgggc 10261 agttccttga gtgctaccgc aatgctcatg cgcttgctgt aaatgtcatc gctcacacgg 10321 tcggccatgt ccgggaatgt caccgatttc ccgccgcgtt cctctaccgc cgtgcgcagc 10381 ttggagccgt tgtaaagctc gaacttcttt tcttccccga agtgtccatt gcgtaccaca 10441 tgaacatccg cattcggaat tgcgtccatg tattccttta gcagttcgag gctatcccgt 10501 tggcggttaa tcacccacag cgtgaccagc ttgcgcttga gttcttcaag cgtgctattc 10561 agtgtttcac cgtaggcgct cacgcccttg ttattccgtg ctgccgtgtt gattaccacg 10621 acgctatccg gcttgctatc gcacaggttc actagctgaa tccatccgtc cgcctcatcc 10681 agattgataa gctccgtttc cgtgctttct ttataggctt tccatacgtc cgggttagag 10741 gtgtcagact caatcagcaa gacgctttcg ccgcgctctt gcaggtaatc aaccgttgcc 10801 atcgtaacaa gcgacttgcc aacgccgccc ttgctgccac cgatcaggta aatgcttttc 10861 gccatgttgc ttactcctta aatgtcgtcg ctgtcaggtt tcggggtgaa tgtcgccttc 10921 gatgtatccg caggcttctt atcggcgggg cggggcgggg gtgtgtctcc cggtgcttgc 10981 accggggctt ttttcgctgg ctttgctctc tgcaagtagc tcttgagcgt tggggttgcg 11041 atgctcagac cttcgccgcg cagcgtctcg gaaatctggt caagcgtgta tccacgcttt 11101 tgcaaagcgg caatctcttt ggatagcacg cgcacggcct cttgcttgct gtgctcctgc 11161 ttcttctttt cgactggcgg catctcacga agctttgaag cgatagcctc tagctgctct 11221 gttgtgtatt tcattctgct aactcctttg ctcactggtt gcggagttga cagcatagcg 11281 ccttttatgc ttgttgcaaa gtgcgtcccg tgcacctaaa ctgctgatat gatagtagag 11341 tggttgagca atacaggatg cggattgtca tacacatgct acgcatgttc ctgccaatcc 11401 ccgcctgccc attcggcgct tcgcttctca cggtctggct catcctgcaa ggggcaagcc 11461 ccttgacccc ggacagtgcg gcgctttcgc ttcttactgt ccaagacaca ccccgccccc 11521 gcccccggcg agaaggagac atgatgccat ttgaaacgca ggggccggaa ccgctggacg 11581 ccgttatcaa tgtgcggctg accgcagcgg agaaggccag attgaaagag gacgccgacc 11641 ttgccgggtt gagcatgtcc gagcttgtcc gccgccgcta cttcggcagg ccgatcattg 11701 ccaacgccga cgccgtgatg ctcaaggagc tgcggcgcat cggtggattg ctgaagcaca 11761 tccacaacga gagcggaggc gtttacagca aggaaacagc cggggcgctt gttgcgctca 11821 aggactactt caggaagcta aacgatgatc gttaagaagg ttccgaatcc gaagaagtcc 11881 gcgagcaagg cgcagcgcat cggccagctt accagctatg tccgttcgcc ggagtcggag 11941 agtccgcagg aaaagtgtct gtatgccggg gcgcggggtt tcatgatgga cgaccccaag 12001 agccagacgg cagagatgat tgcactctca caagaagccg tgcgcagcaa ggacaccatc 12061 aatcactacg tcttgagttg gcgcgaaggc gaacagccaa gcccggagca agtagaggaa 12121 gcggttagca tcttcatgga tgagcttggg tggaaagacc accaagccat ctatggcctt 12181 cattccgaca cggacaatat ccacctgcac atcgtcatca atcgggtgca tcccgaaacg 12241 ctcaagattg ttgagaagaa ccgtggattt gatattgaac tggcacacaa agccattgca 12301 cggattgagc acgcccaagg ctggcagcgt gagcaaaacg ggcgctatca ggtgttggag 12361 aacggggagc taggccgagc gccatacgac ccggaaaagc cacgccagcc cgaccagaag 12421 aagcgggaca tggagaaccg caccggggag aagtcagccc accgcatcgc catcgaggac 12481 ggcgcagcca tcatcaagca ggcgcagacg tgggagcagt tgcaccgcga gttagcagcc 12541 aaggggatgc gctacgaaaa gaccggcagc ggggcgaccg tgtttgtcgg ggatgttggc 12601 gtgaaggcca gcgatgtaga ccgcaacgcc agccttgcca agatgcagaa gcggctaggt 12661 gaataccagc cagcgccgca gcgtcagcag gtagcgccgc gcgagccgga gccgatcaag 12721 ccagacgtgc cgggatggaa ggactacatc accgggcgca aggcacacta cgccgagaag 12781 aacgcggaca agctggcaca ggacaagcgg caggagcagg agcgcaagca acttgccgag 12841 cagcagaagg cccgccgcga tgaactcatg cgcggaaact ggaaaggcaa gggcgaagtg 12901 ctcaacgcca tgcgcagcgt gattgccgcc gagcaggccg cagagaaagc ggccttgaag 12961 gagaagcacc agaaggagcg cgagcagcac cggcagcggt tccgacccta tcccgacctg 13021 gagcagtggc agcggatgca gaaaagcccg gagcttgccg agcagtggcg acaccgggcc 13081 agcgagccgc agcgcatcga gggcgaccgc agcgagccgc cgacgccgcg agacattcgc 13141 gcctatcagc ctgaaatcgt gggccagcag gtgcattaca gccggaagga agaagcgggg 13201 cggggcgggg gtgtgtcttt cgtggacaag ggcaaaagca tcgatattca cgactggcgc 13261 aaccgggaca gcacgcttgc cgcgttacag ctatcggcgc agaagtgggg cagcttcacc 13321 gtgatgggga acgacgaata caaggccatg tgcgggaagt tagcagcaga gcacggcttc 13381 aagatcacaa atccagagct tcaggagagc atccagcagg agcggcagcg gatacagcag 13441 gaaagggtgc aggcgatgaa atcggagcag ctaaagcagt tcgagcggta cgcggaagcg 13501 gtaggcgcag agcgttaccg ggtaacgtct atcaagatgc gcgaggacgg cagcaagcaa 13561 accttcatcc tcgacaagaa ggacggcatc acgcggggct tcacaccgca ggagatcgag 13621 cagcgcacgc cggagatgca gcgcctacag cgccggggcg aaaacctcta ctacacgccg 13681 ctatcggaca agaagcatca catcctcatc gacgacatga accgggagaa gctggagcgg 13741 cttatccgcg acggctacca gcccgccgcc gtgcttgaat ccagccccgg caactatcag 13801 gccatcatca ccgtgccgaa gctggggacg gcccacgata aggacgtggg caaccggctg 13861 agcgatgccc tgaaccgtga atacggcgac ccgaagctat cgggagccat ccacccgcac 13921 cgtgcacccg gctacgagaa ccgcaagccc aagcaccagc gggaggacgg cagctatccc 13981 gaggtgcgct tgctcaaggc cgagcgccgg gagtgcatca aggcgctggc cttgtccagc 14041 cagatcgacg ccgagtatca gcggcaagcg gccttgaagg cgcagcagcc cgagcgcagc 14101 aaagccaagc ccgccttgga gcttgcagcg gccagcggca gcgcgatcga cgcctaccag 14161 cggcattatc gcgacgtgct caagcggcag cgtggcggcg aggtggactt gtcccgcttg 14221 gattccatga ttgccgtgcg tatgcgcgtc accggccacg atcaagccgc catcgagggc 14281 gctatccgcc agtgcgcccc ggccacccgg cagaaggacg agggccgcga ctggaacgac 14341 tacgcgcagc gcaccgcccg ctatgcctac agcgccgcag gcgaccgcca agccgccgag 14401 cttgggaagt accgccagca gtgggagaag ctggaagggc gcgagcctgt acgccagcag 14461 gagcaggcca aggcgcagaa gatcgagcgc gataactcgc cggggatgag tctttaaata 14521 cctgttgcat gtaacttgcg aggtgcgtac aatgttgcat gtaacttaat tggaggcttg 14581 gaaatggcaa cagcacacgt taatcagcgg gttcagaagc atcgcgatgc attacgcatg 14641 gcggggcttc gtccggttca aatctgggtg ccggatacgc gccgccccga cttcgcggag 14701 gagtgccgcc gccaatgcct gcttgtggct caggccgaca gcgccgacac cgccatgcag 14761 cagttcatgg atgaagcctt ggcagacgtg gacggctgga cggaatgatg cgcggcgacc 14821 ttgtgaccat cgccatgcag ggcgactttg gcaagccacg gcctgcgctt gtggttcagg 14881 ccaaccagtt cagcgagcac ggcagcgtta ccgtgctgcc tgtcaccagc acacttaatg 14941 ccgcgccgtt gctgcgcgtc acggttcagc cgagcgcaga gaacggtttg cagaagccat 15001 cgcaggtgat ggtcgacaag gcgatgacgg tgctgcgcag caaggtaggg ccagctttcg 15061 ggcgcatcga cgcggacgca ctgcttgagg ttgagcgttg cttggccgtg ttcttgggga 15121 ttgcgaaatg aaaacgatct acaccattcc cgccccggat tccttgggcg cgatgatcga 15181 ggtctacggc gagccggaaa acgcttggta cgagtggcgc atcatcgacg gcgggcgcac 15241 cgtgcgggac accggcacgg aaggccacag cgcctttcag ggacgccagt acgggcaagc 15301 agagattgcc ctacgtgatg cgctcatgtt cgcttctggc ctgcccatcg acggcgacac 15361 gccgaccgat gccgccagtc tggaagcggg ctatgccgcc aaggcgaagg cagaagcggc 15421 gcggcagggt gcgggaacac ccggcagcgc ctaaccataa ccgttctaca caggagaaca 15481 gc //