LOCUS       LC499780                 779 bp    DNA     linear   PLN 01-JUN-2021
DEFINITION  Trichoderma harzianum THIF08 cos gene for carbonyl sulfide
            hydrolase, complete cds.
ACCESSION   LC499780
VERSION     LC499780.1
KEYWORDS    .
SOURCE      Trichoderma harzianum
  ORGANISM  Trichoderma harzianum
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae;
            Trichoderma.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Matsushita,Y., Katayama,Y. and Masaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2019)
            Contact:Yasuhiko Matsushita
            Tokyo University of Agriculture and Technology, Gene Research
            Center; 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan
REFERENCE   2
  AUTHORS   Masaki,Y., Iizuka,R., Kato,H., Kojima,Y., Ogawa,T., Yoshida,M.,
            Matsushita,Y. and Katayama,Y.
  TITLE     Fungal Carbonyl Sulfide Hydrolase of Trichoderma harzianum Strain
            THIF08 and Its Relationship with Clade D beta-Carbonic Anhydrases
  JOURNAL   Microbes Environ. 36, ME20058 (2021)
  REMARK    Publication Status: Online-Only
            DOI:10.1264/jsme2.ME20058
COMMENT     
FEATURES             Location/Qualifiers
     source          1..779
                     /collected_by="Yoko Katayama"
                     /collection_date="2008"
                     /db_xref="taxon:5544"
                     /geo_loc_name="Japan: Tochigi, Karasawa-yama area"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /note="Each primer-binding site comes just outside the
                     registered sequence."
                     /organism="Trichoderma harzianum"
                     /PCR_primers="fwd_name: 20kd_F01, fwd_seq:
                     ggaaccgtttgctccaagtgagg, rev_name: 20kd_R02, rev_seq:
                     ctgagatgaactggtggtattaatctac"
                     /strain="THIF08"
     CDS             join(91..176,279..456,523..750)
                     /codon_start=1
                     /gene="cos"
                     /product="carbonyl sulfide hydrolase"
                     /protein_id="BBN51267.1"
                     /transl_table=1
                     /translation="MTVASEFEVANQQYVATFDKADLPMPPGRKVFVLTCMDARLDPA
                     KFLGLEEGHAHVYRNAGGRAAEALRSLIISQQALGTEEVVVIHHTDCGMLLIHEEEFR
                     NTVKKNTGEDVSHIAFLTIQDLQKSVKTDVELLRKNAAIKNVPISGYIFDVKTGKINK
                     VDV"
BASE COUNT          202 a          189 c          172 g          216 t
ORIGIN      
        1 ttctcttcat ctccaagcca tatcaagaca gtcttcaaga cattgcattt ctctttccat
       61 caccttattt agcaacacac actatcgaaa atgaccgtcg ccagcgagtt tgaagttgcc
      121 aaccagcaat atgttgctac atttgacaag gccgatctgc ctatgcctcc cggccggtgc
      181 gtttcatcta gctttcatta ttctctacag caatccatct tttctacaca gcaacttgag
      241 gattctcatg atttgctaat ttgagatgag atacttagaa aggtttttgt cttgacttgc
      301 atggacgctc gtctggaccc agccaaattc cttggccttg aagagggcca cgcccacgtc
      361 taccgcaatg ctggaggacg agctgccgag gcgctgcgct ccttgatcat ttctcagcaa
      421 gctctgggaa ccgaagaagt cgttgttatc catcacgtat gaattgtctc cctagaagct
      481 ttgatgcaca tagaattggt tagctgacaa gttatttata agaccgactg cggtatgctt
      541 ctcatccacg aggaggagtt ccgcaacact gtcaagaaga acactggcga ggatgtcagc
      601 cacattgcat tcttgaccat ccaggacctg cagaagagcg tcaagactga tgtcgagctt
      661 ttgaggaaga atgctgcgat caagaatgtg cctatttcgg gctacatctt tgatgtcaag
      721 acgggcaaga ttaacaaggt tgatgtttaa gcgcatgtac gtctagaggc aaattatat
//