LOCUS       STRSPXB                 2045 bp    DNA     linear   BCT 17-JAN-1997
DEFINITION  Streptococcus pneumoniae pyruvate oxidase (spxB) gene, complete
            cds.
ACCESSION   L39074
VERSION     L39074.1
KEYWORDS    pyruvate oxidase; spxB gene.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Spellerberg,B., Cundell,D.R., Sandros,J., Pearce,B.J.,
            Idanpaan-Heikkila,I., Rosenow,C. and Masure,H.R.
  TITLE     Pyruvate oxidase, as a determinant of virulence in Streptococcus
            pneumoniae
  JOURNAL   Mol. Microbiol. 19 (4), 803-813 (1996)
   PUBMED   8820650
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="R6x"
                     /db_xref="taxon:1313"
     regulatory      140..145
                     /regulatory_class="ribosome_binding_site"
     gene            154..1929
                     /gene="spxB"
     CDS             154..1929
                     /gene="spxB"
                     /note="TPP binding consensus sequence (X5,Gly,Asp,Gly,
                     Y25-28,Asn) is residues 435-468; putative"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="AAB40976.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFAFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSLDVGSTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDTPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYGKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKGGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
BASE COUNT          648 a          433 c          425 g          539 t
ORIGIN      
        1 ctgtattaga atagagaata gagagttttg agcagatttt tagaaaagtc agcataaata
       61 tgatacagtg gaatagtaaa aatttggaga acgtttccaa ttctatgtaa tcgtattctc
      121 caagtttaaa aaaattgaag gagagttatc attatgactc aagggaaaat tactgcatct
      181 gcagcaatgc ttaacgtatt gaaaacatgg ggcgtagata caatctacgg tatcccatca
      241 ggaacactca gctcattgat ggacgctttg gctgaagaca aagatatccg cttcttacaa
      301 gttcgccacg aagagacagg tgctcttgca gcggttatgc aagctaaatt cggcggctca
      361 atcggggttg cagttggttc aggtggtcca ggtgcgactc acttgattaa cggtgtttac
      421 gatgcagcta tggataacac tccattccta gcgatccttg gatcacgtcc agttaacgaa
      481 ttgaacatgg atgctttcca agagcttaac caaaacccaa tgtacaacgg tatcgctgtt
      541 tacaacaaac gtgtagctta cgctgagcaa ttgccaaaag taattgacga agcctgccgt
      601 gctgcaattt ctaaaaaagg tccagctgtt gttgaaattc cagtaaactt cggtttccaa
      661 gaaatcgacg aaaactcata ctacggttca ggttcatacg aacgctcatt catcgctcct
      721 gctttgaacg aagttgaaat cgacaaagct gttgaaatct tgaacaatgc tgaacgccca
      781 gttatctatg ctggatttgg tggtgttaaa gctggtgaag tgattactga attgtcacgt
      841 aaaatcaaag caccaatcat cacaactggt aaaaactttg aagctttcga atggaactat
      901 gaaggtttga caggttctgc ttaccgtgtt ggttggaaac cagccaacga agtggtcttt
      961 gaagcagaca cagttctttt ccttggttca aacttcgcat ttgctgaagt ttacgaagca
     1021 ttcaagaaca ctgaaaaatt catacaagtc gatatcgacc cttacaaact tggtaaacgt
     1081 catgcccttg acgcttcaat ccttggtgat gctggtcaag cagctaaagc tatccttgac
     1141 aaagtaaacc cagttgaatc aactccatgg tggcgtgcaa acgttaagaa caaccaaaac
     1201 tggcgtgatt acatgaacaa actcgaaggt aaaactgagg gtgaattgca attgtatcaa
     1261 gtttacaatg caatcaacaa acatgctgat caagacgcta tctactcact cgacgtcggt
     1321 agcactactc aaacatctac tcgtcacctc cacatgacac ctaagaatat gtggcgtaca
     1381 tctccgctct ttgcgacaat gggtattgcc cttcctggtg gtatcgctgc taagaaagac
     1441 actccagatc gccaagtatg gaacatcatg ggtgatggag cattcaacat gtgctaccca
     1501 gacgttatca caaacgttca atacgacctt ccagttatca accttgtctt ctcaaatgct
     1561 gagtacggct tcatcaagaa caaatacgaa gatacaaaca aacacttgtt tggtgtagac
     1621 ttcacaaacg ctgactacgg taaaattgcg gaagctcaag gagctgttgg attcacagtt
     1681 gaccgtatcg aagacatcga tgcagttgtt gcagaagctg ttaaattgaa caaaggaggt
     1741 aaaactgttg tcatcgatgc tcgcatcact caacaccgtc cacttccagt agaagtactt
     1801 gaattggatc caaaacttca ctcagaagaa gctatcaaag ccttcaagga aaaatacgaa
     1861 gcagaagaac tcgtaccatt ccgtctcttc ttggaagaag aaggattgca atcacgcgca
     1921 attaaataat tcctctcgcc gaaaatcaaa tatgaaactt gtagacttta ttctttgatt
     1981 ttcatagcag cagaacttta aaagatcgga gcaatccggt ctttcctatg gaggagagta
     2041 acatg
//