LOCUS PSEMETH 9117 bp DNA linear BCT 02-MAY-1996 DEFINITION Pseudomonas fluorescens methyltransferase gene, sensor kinase gene, phosphatidylglycerophosphate synthase (pgsA) gene, UVR excinuclease subunit C (uvrC) gene, response regulator/transcription activator gene, complete cds. ACCESSION L29642 VERSION L29642.1 KEYWORDS UVR excinuclease subunit C; glyW gene; methyltransferase; pgsA gene; phosphatidylglycerophosphate synthase; response regulator; sensor kinase; transcription activator; transfer RNA-Gly; uvrC gene. SOURCE Pseudomonas fluorescens ORGANISM Pseudomonas fluorescens Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 9117) AUTHORS Gaffney,T.D., Lam,S.T., Ligon,J., Gates,K., Frazelle,A., Di Maio,J., Hill,S., Goodwin,S., Torkewitz,N., Allshouse,A.M. et al. TITLE Global regulation of expression of antifungal factors by a Pseudomonas fluorescens biological control strain JOURNAL Mol. Plant Microbe Interact. 7 (4), 455-463 (1994) PUBMED 8075420 FEATURES Location/Qualifiers source 1..9117 /organism="Pseudomonas fluorescens" /mol_type="genomic DNA" /strain="BL915" /db_xref="taxon:294" CDS 210..1688 /note="homology with E. coli cheR and Myxococcus xanthus frzF genes" /codon_start=1 /transl_table=11 /product="methyltransferase" /protein_id="AAA98755.1" /translation="MLLRTGTGHDFKHYKRATVLRRIERPLQVTAQPDLAAYHDYLQM HPEETKALLGDMLIGVTNFFRDREAFEALERNVIPALVKSLQDSQPHREDVRIWSAGC STGEEAYSLAIVASEQMALEACNAKLQVFATDIDDRAIAQGRKGVYPEAIVTDVPPQR MRQYFSRENQHYRVRKEIREKVLFAKHSLLADPPFSQIDLIVCRNLLIYLDRDVQREI LQMFHFALRPGGYLFLGSSESADGCQDLFVPVDKRNRIFRVRPNSATVRRAPTMPRRR TCAPSAAPTPWKPSVSRKTSFADIHLRALEKCAPPSMIVDANADILHMSEGAGRFLRY VAGEITRNLLTLIQPELRLELRTTLFQVQQSGVAVTAAGCASSGKRSLVSSTSQPAPS RTRKPTTNMCWWCSRRPRPTHGSCARPAPARRKTRCWPTSSGSCSGPNCTCRTPSSNR KSPARSSRRRTKKCRRSMKSCARPPKSWKPARKSCSRSMKSC" CDS 1906..3633 /note="homology with a variety of bacterial sensor kinases of two-component regulatory systems, such as rcsC and cheA of E. coli, frzE of Myxococcus xanthus, and bvgS of B. pertussis" /codon_start=1 /transl_table=11 /product="sensor kinase" /protein_id="AAA98756.1" /translation="MAEDAATVFESLSMIEREVNSDDQRWYIARLLPYRSSEDHIDGT VLTFIDITKRRLAEEELRLGEERMRLVAESTHDFAIIILDNQGLITDWNTGAQLIFGY TKDEVLGAYYDLIFAPEDRAGGVPESELLTAREHGRSDDERWHIRKDGERFFCSGEVT RLKGDSLQGYVKIARDLTGHKRMQDEQNQKLMETQTHSHLKDEFFAVMSHELKHPLNL IQLNAELLRRLPTTKAAAPALKAVNTICEAVSSQARIIDDLLDVRRLRTGKLKLKKQP VDLGRILQDIHTVVLSEGHRCQVTLQVPLPPQPPLMIDADATRLEQVIWNLVNNALKF TPANGLVQLIAQRVEDKAHVDVIDSGVGLAEEDQNKVFDLFGQAANQHGTHQRDGLGI GLSLVRQLVEAHGGSVSVQSKGLGQGCTFTVLLPLSHPNDSAPKQPASRGVERLAGIK VLLVDDSREVMEVLQLLLEMEGAQVEAFHDPLQALGNARNNSYDLIISDIGMPIMNGY ELMQNLRQIAHLHHTPAIALTGYGASSDQKKSQHAGFDRHVSKPVAQDPLIDLIRELC SQGLRSAEH" gene complement(4616..4691) /gene="glyW" tRNA complement(4616..4691) /gene="glyW" /product="tRNA-Gly" /note="codon recognized: GCA" gene complement(4731..5318) /gene="pgsA" CDS complement(4731..5318) /gene="pgsA" /EC_number="2.7.8.5" /note="product is also known as CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferese" /codon_start=1 /transl_table=11 /product="phosphatidylglycerophosphate synthase" /protein_id="AAA98757.1" /translation="MNIPNLITVLRVLLIPIFILLFYLPYNWSYMAASSVFAFAAATD WLDGYLARRLEQSTPFGAFLDPVADKLMVAVALVLLVQEHGNLWLTLPAAVIIGREIV VSALREWMAELGRTRPGGRCPTWANRKTAAQMLALVILLANPPAFTFWVLLGYAFLLI AGGLTLWSMLQYLRAAWPHLKTDGRKEIKLFESRG" gene complement(5574..7397) /gene="uvrC" CDS complement(5574..7397) /gene="uvrC" /codon_start=1 /transl_table=11 /product="UVR excinuclease subunit C" /protein_id="AAA98758.1" /translation="MTDPFDPSAFLSTCSGRPGVYRMFDSDTRLLYVGKAKNLKSRLA SYFRKTGLAPKTAALVGRIAQIETTITANETEALLLEQTLIKEWRPPYNILLRDDKSY PYVFLSDGEFPRFSIHRGAKKQKGKYFGPYPSAGAIRESLSLLQKTFMVRQCEDSYYK NRTRPCLQYQIKRCKAPCVGLVEAEVYAEDVRHSVMFLEGRSNALTDELSAGMEQAAS TLDFEKAAELRDQISLLRRVQDQQSMEGGSGDVDVVAAFVNPGGACVHLISVRGGRVL GSKNFFPQVGIEEEVAEVMSAFLGQYFLSSPERDLPSELIVNVVHEDFPALIAAIQEL RGRELSISHRVRGTRARWQQLAVTNAEQALSARLANRQHVAARFEALAEVLNLDEPPQ RLECYDISHSSGEATVASCVVFGPEGPLKSDYRRYNIEGVTAGDDYAAMHQALTRRFS KLKDGEGKLPDILLVDGGKGQLSMARDVLNELAVPDLILLGVAKGATRKTGFETLYLN DAAHEFTLKGDSPALHLIQQIRDEAHRFAITGHRARRGKTRRTSTLEDVAGVGPKRRR DLLKHFGGLQELSRASIEEIAKAPGISKKLAELIYANLHSE" CDS complement(7400..8041) /note="homology with uvrY of E. coli and gacA of P. fluorescens CHA0; member of FixJ class of response regulators" /codon_start=1 /transl_table=11 /product="response regulator/transcription activator" /protein_id="AAA98759.1" /translation="MIRVLVVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLLKAR ELKPDVVLMDVKMPGIGGLEATRKLLRSHPDIKVVAVTVCEEDPFPTRLLQAGAAGYL TKGAGLNEMVQAIRLVFAGQRYISPQIAQQLVFKSFQPSSDSPFDALSEREIQIALMI VGCQKVQIISDKLCLSPKTVNTYRYRIFEKLSISSDVELTLLAVRHGMVDASA" BASE COUNT 1865 a 2876 c 2667 g 1709 t ORIGIN 1 gaattcgatg acatgccgcg cgccggcatc gacacgcaaa tggtcgacct ggtgctgccg 61 gtggtcgaaa tgccgcagaa gctgctggag ctgtggcgca actctcagct catcaccctg 121 ccgaccgcca acgatccgca aatcaaggtc tcggcgccgg tgtccaaacg cgatgccgcg 181 gcggcgaaca gcagctgcaa gacatcctga tgctgttgcg caccggcacc ggccatgact 241 tcaagcatta caagcgcgcc acggtgctgc ggcggatcga gcgcccgctg caggtcaccg 301 cccagccgga cctcgccgcc tatcacgatt acctgcagat gcaccctgaa gaaaccaagg 361 cgctgctggg cgacatgctg atcggcgtga ccaatttctt tcgcgaccgc gaggccttcg 421 aagccctgga gcgcaatgtc attcctgccc tggtgaagtc cttgcaggac agccaaccgc 481 accgtgaaga cgtgcgcatc tggtccgccg gctgctccac gggtgaagag gcctatagcc 541 tggcaatcgt cgccagcgag cagatggccc tggaggcctg caacgccaag ctgcaggtat 601 tcgcgaccga tatcgacgat cgtgccatcg cccagggacg caagggggtc tatcccgaag 661 cgatcgttac cgatgtgcct ccgcagcgca tgcgccagta cttttcccgg gaaaaccagc 721 attaccgggt gcgcaaggag attcgcgaaa aggtgctgtt cgccaagcac agcctgctgg 781 cggatccgcc attttcgcag atcgacttga tcgtctgccg taacctgctg atctacctgg 841 accgcgacgt gcaacgggag atcctgcaga tgttccactt cgccctgcgt cctggaggct 901 acctgttcct cggttcctcc gaatccgcgg acggctgcca ggatctgttc gtgccggtcg 961 acaagcgcaa ccgcattttc cgggtacggc ccaactcggc cacggttcgc cgcgcgccca 1021 ccatgccgcg acggcgtaca tgcgcaccat cggcagcccc caccccgtgg aaaccaagtg 1081 tctcgcgcaa aacctcgttc gccgacatcc accttcgcgc cctggaaaag tgcgcgccgc 1141 cgagcatgat cgtcgatgcc aacgccgaca tcctgcacat gagcgaaggc gccggccggt 1201 tcctgcgcta tgtcgcgggg gaaatcaccc gcaacctgct gaccctgatc cagcccgagc 1261 tgcgccttga actgcgcacc acgctgttcc aggtgcaaca gtccggtgtt gcggtgaccg 1321 ccgccgggtg cgcatcgagc gggaaaagaa gccttgtttc atcgacctca cagcccgccc 1381 cttcaaggac gaggaaaccg acaacgaata tgtgctggtg gtgttcgagg agaccgaggc 1441 cgacccacgg gagctgcgcg agaccagcgc cagccagacg gaaaaccaga tgctggccaa 1501 cctcgagcgg gagttgcagc ggaccaaatt gcacctgcag gacaccatcg agcaatcgga 1561 agtctccagc gaggagctca aggcgtcgaa cgaagaaatg caggcgctca atgaagagct 1621 gcgctcggcc accgaagagc tggaaaccag caaggaagag ttgcagtcga tcaatgaaga 1681 gctgctgacg gtcaattacg agctgaaaac caaggtcgag gaaaccgaca agatcaacga 1741 ctacctgacc aacctgatcg cctccaccga catcgccacg gtgttcgtcg accgcaacat 1801 gcgcatccgc tggttcaccc cgcgcgccac cgacattttc agcatgctgc cggtggacac 1861 cgacgctcat tactggacat cacccaccgc ctgaactacc cggaaatggc cgaggacgcc 1921 gcgaccgtgt tcgagtcgtt gagcatgatc gagcgtgaag tcaacagcga cgatcagcgc 1981 tggtacatcg cacgcctgtt gccctatcgc tccagcgaag accatatcga cggcaccgtg 2041 ctgaccttca tcgatatcac caagcgccgg ctggccgagg aggaactgcg cctgggcgaa 2101 gaacgcatgc gcctggtcgc cgaaagcacc catgatttcg ccatcatcat cctcgacaac 2161 cagggcctca tcaccgactg gaacaccggg gcgcaactga tcttcggcta taccaaggac 2221 gaagtgctgg gcgcctatta cgacctgatt ttcgcgcctg aggaccgcgc cggcggcgtg 2281 ccggaaagcg agctgctcac cgcccgcgaa cacggccgca gcgacgatga acgctggcat 2341 atacgcaagg acggcgagcg ctttttctgc agcggcgaag tcacgcggct caagggtgac 2401 agcctgcaag gctacgtgaa aatagcccgc gacctgacgg gccacaaacg catgcaggac 2461 gagcagaacc agaagctgat ggagacccag acccacagcc acctcaagga tgagtttttc 2521 gcggtgatgt cccatgaact caagcatccg ctcaacctga tccagctcaa cgccgagttg 2581 ctgcgtcgcc tgccgacgac caaggcggcc gcccctgccc tcaaggcggt caataccatt 2641 tgcgaggctg tctccagcca ggcgcggatc atcgacgacc tgctggatgt gcggcgtttg 2701 cgcaccggca agctcaagct gaagaaacag ccggtggatc ttggccggat cctgcaggac 2761 atccataccg tggtgctcag cgaagggcat cgctgccagg tgacgctgca agtgccgttg 2821 ccaccgcaac cgccgttaat gatcgatgcc gatgcgacgc ggctggagca ggtgatctgg 2881 aacctggtga acaacgccct gaaattcacc ccggccaatg gcttggtcca gttgatcgcc 2941 cagcgggtcg aggataaggc gcacgtggat gtcatcgaca gcggcgtggg cctggccgag 3001 gaagaccaga acaaggtgtt cgaccttttc ggccaggcgg ccaaccagca cggcactcat 3061 caacgcgacg ggctgggcat cggcctgtca ctggtgcgcc agctggtgga agcccacggc 3121 ggctcggtca gcgtgcagtc gaaggggctg ggccagggat gcacctttac cgtgctcttg 3181 cccctgagcc accccaacga cagcgctccc aaacagcccg cgtcgcgggg tgtcgaacgc 3241 cttgccggca tcaaggtgct gctggtggac gactcgcggg aagtcatgga agtcctgcaa 3301 ctgctgctgg agatggaggg cgcgcaagtc gaggccttcc acgacccgct gcaggccttg 3361 ggcaatgcca ggaacaacag ttacgacctg atcatttcag acatcggcat gccgattatg 3421 aacggctacg aactgatgca gaacctgcgc cagatcgctc acctgcacca tacgccagcg 3481 attgcgctga ccggttacgg cgccagcagc gaccagaaga agtcccagca tgcgggattc 3541 gatcggcatg tgagcaaacc cgtggctcag gacccgctga tcgacctgat cagggagctg 3601 tgcagccagg gcttgcgctc ggctgagcac tgatggtcta gacccggcga acccacctcg 3661 tcggccttga gcgcggcgag cgccattgcc tgctgggcag ctattcacgc ttgcggatcg 3721 tcgcgcctgc gggccaccgc ctctttgatg gcttgctcat aggcggcgtt ggcctggtcc 3781 ttgagcttga gccaatcgtc ccaatcgatc acgccgttgc gcagcaactc ctcggccgcg 3841 cttaacagcg cctgatgcca ggcgtccggc gagccggaac ggtagtcacg gtcttccagc 3901 aggccttgcc aggcgtccag ttccggtgtc ttgcgttcat tgaccatggc agccacggcc 3961 tttgttcatt gccgataaat cggcgagtgg gtggtgggtt tctcggatat gcgccctgtc 4021 ctgctcgaga acggccaggc cgggacattg ctcaacggtc agcgaccgga tggagctcga 4081 gcggcatgcc atcgaccagc gtcaaggtca ggttctcgat ggtgccggcg atccggtcct 4141 tgaataccgg ttcgccgtcc ggatccaact catcgtagaa aaagcgcgtg ccttcgagcc 4201 agccaatggt cgtttgcagg tccggcccca ggtaatactt gccgtcaagg aaaaacccgg 4261 taaagggctc cacccgctcg cgattctcaa tgacataacg tattccagcg tgcatacctg 4321 tcgatttatc gagcatggcg tcgatctccc agcagatgaa tccggtagac cgcgtggctt 4381 tttcactgtt ccttttgatt gcccgcccga cgctggcgag ccttgctcgc gcgtcctggc 4441 cgcattgcgc ggcgaatggg cgacgtcgaa tccgatctgc aagtgcccag ctagcggccc 4501 ggccacggca atacgggctt caggtacggc ttagaaagaa gaatgacgat tggctcgaca 4561 tattttttgg cgcaaaaaaa aatggacctc ttttcagagg tccattttta atatttggag 4621 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga 4681 gctattcccg cgtcttggtg gtgtgcattt tatagaaatt cgaaactgcg tcaacccctt 4741 gattcaaaaa gttttatttc ttttctacca tcggtcttca ggtgcggcca ggcagcgcgc 4801 aggtactgca acatcgacca cagggtcagc cctccggcga tcagcaggaa ggcataaccc 4861 agcagcaccc agaaggtgaa ggccggcgga ttggccagca ggatcaccag cgccagcatc 4921 tgcgcggcag ttttccgatt tgcccatgtt ggacaccggc cacctgggcg cgtgcgcccg 4981 agctcggcca tccactcgcg aagggcggac accacgattt cacgcccgat gatcaccgct 5041 gccggcaggg tcagccacag gttgccgtgc tcttgcacca gcagcaccag ggccaccgcc 5101 accatcaact tgtcggccac cggatcgagg aaggccccga acggcgtgct ctgctccaga 5161 cgccgcgcca ggtagccatc aagccagtcg gtggccgcgg cgaacgcaaa gacggaactg 5221 gcggccatgt agctccagtt gtaaggcagg taaaacagca aaatgaagat cgggatgagc 5281 agaacgcgta gaacggtgat cagattaggg atattcatcg gcacaactgg ctacgaggtg 5341 agtggcaatc tactcggaaa agacagcaga tgaggtagca cggccattct acgggcttct 5401 gccacagcgt gtctaacact gttccaagac ttcgggccgc tcgaaagagc aacttcagaa 5461 ggtctacacg cgcaaaataa gacattcagt tcttctgtaa gtaccgtgta gatcgggatc 5521 tatcagcggt gccccgccaa aaaggaagcc ttgaagcttc cttgagcgct cccctactcg 5581 ctatgcaagt tcgcataaat cagctcagcg agctttttac tgatccccgg cgctttggcg 5641 atctcctcaa tgctggcgcg agacagttcc tgcaacccac caaagtgttt caacaggtcg 5701 cggcggcgct tggggccgac gccggccacg tcttcgaggg tcgaagtgcg gcgggtcttt 5761 ccgcgacggg cgcggtggcc agtgatggcg aaacggtgag cctcgtcgcg gatctgctgg 5821 atcaggtgca gcgccggcga gtcgcccttg agggtgaact catgggcggc atcgttgagg 5881 tagagggtct cgaaaccggt cttgcgcgtc gcacccttgg ccacacccag caggatcagg 5941 tcaggcaccg ccaactcgtt gagcacgtcg cgggccatgg acagctggcc cttgccgccg 6001 tccaccagca ggatgtccgg caacttgccc tcgccgtcct tgagtttgct gaagcgtcgt 6061 gtcagggcct ggtgcatcgc cgcatagtca tcgccggcgg tgacgccttc gatgttgtag 6121 cgccgatagt cggacttcag cggcccttcc ggaccgaaca ccacgcagga cgccacggtc 6181 gcctcgccgc tggagtggct gatgtcgtag cactccaggc gttgcggtgg ctcgtccagg 6241 ttcagcactt cggccagggc ctcgaaacgc gccgccacat gctgccggtt ggccaggcgc 6301 gcactcagcg cctgttcggc gttggtcact gccaattgct gccagcgcgc ccgcgtaccg 6361 cgcacccggt ggctgatgct cagctcgcgg ccacgcagct cctggatcgc cgcgatcagg 6421 gccgggaaat cctcatggac cacgttgacg atcagctcgc tgggcaggtc gcgctccggg 6481 ctgctgagaa agtactggcc gaggaaggcc gacatgactt cggccacctc ttcctcgatg 6541 cccacctggg gaaagaagtt cttgctgccc agcacccgcc cgccccgcac gctgatcagg 6601 tgcacacagg cgccgcccgg gttgacgaag gccgcgacca cgtcgacgtc gccactgccg 6661 ccttccatgc tctgctggtc ctggacccgt cgcagcaggg aaatctggtc gcgcagctca 6721 gcggcctttt cgaagtccag ggtgctggcc gcctgctcca tgccggccga cagttcgtcg 6781 gtcagggcat tgctgcggcc ttcgaggaac atcaccgagt ggcgcacatc ctcggcgtac 6841 acctcggcct ccaccaggcc gacgcacggc gccttgcagc gcttgatctg atattgcaga 6901 catggccggg tgcggttctt gtagtagctg tcctcgcact ggcggaccat gaaggtcttt 6961 tgcagcaggc tgaggctctc gcgaatggcc ccggcgctgg ggtacgggcc gaaatacttg 7021 cccttctgct tcttcgcccc acgatggatg ctgaaacgcg gaaactcgcc gtccgagaga 7081 aacacatagg gataggactt atcgtcgcgc agcaggatgt tgtacggcgg ccgccattcc 7141 ttgatcagcg tctgctcgag cagcagggct tcggtctcgt tggcggtgat ggtggtttcg 7201 atctgcgcga tgcgccccac cagggcagcg gtcttgggcg ccaggccggt cttgcgaaag 7261 tagctggcca ggcggctctt caggttcttg gctttaccga cgtacagcag acgcgtatcg 7321 ctgtcgaaca tgcgatacac gccaggacgg ccactgcagg tggaaagaaa agcactggga 7381 tcaaacgggt cggtcattgt caggcactgg catcgaccat gccgtggcga accgccagca 7441 atgtcagttc aacatcgctg ctgatcgaga gcttttcgaa gatgcggtaa cggtaggtat 7501 taacggtttt cggagacagg cacagcttgt cggagatgat ctgcactttc tggcagccga 7561 caatcatcag cgcgatctgg atttcccgct cggacaaagc atcgaacggt gaatcactgg 7621 aaggctggaa tgacttgaac accaactgct gggcaatttg cgggctgatg taacgctggc 7681 cggcaaacac caggcgaatg gcctgcacca tttcattgag gcccgccccc ttggtcaggt 7741 aacccgccgc gccggcttgc agcaagcggg tcgggaacgg atcttcttca cacacggtga 7801 cggccacgac tttgatatcc gggtgactgc gcaacaattt gcgcgtggct tcaagaccgc 7861 cgatcccggg catcttgacg tccatgagga ccacatcggg tttcaactcc cgggccttga 7921 gcagggattc ctcccctgac tcggcctggc cgaccacttg caggccatcg atgtcagcca 7981 gcattcgtgt aatacctgta cgaacgagat catggtcatc gactactagc accctaatca 8041 agcagacacc tcgcgatttg ggtcttatag gttgccggac accttagcaa aaaagcagcg 8101 tgctgaccta atgacaaaca ccatataaaa agcacttgtt catcaggggt atccggtgga 8161 tggttgatgc gctgcgaacg ccctgcccta aggctctcgg gcgtccgcct ttcttttcat 8221 gaggctggaa accgaaagct cggcaagggt atgggtcagg tgccggatcg cgtcctgatc 8281 ttccttgtac aaggcccgga taactgacga ggcttttctc ttcgctctgg ctgagattct 8341 cagccctggt cggggtccgc ctgccggtca ctatataaag gacatcgacc ccttttgtcg 8401 ccacccggga caagtaatcc gccttgggca cacggtctcc actttcatat ctgccttggg 8461 cattggcttc cacgccgcca acttcaccaa attttttctg cgacaacccc aggcgttccc 8521 tttcctgtcg tcaaccgcga accgattcca ctcatttgga tgtatgatcc ttttttatgc 8581 acccctaggg gtgttacacc cttcaagcat tgaacaaatt tgaacggttt tgaactatgc 8641 ccggttatcc gcactgccgc acaagccaag gcctggcttg aacatcaagg taaatcggtt 8701 caacagttcg ctcgtgatca cggggtcgat ccagccacca cttatcaggt attggctggc 8761 cgcaagaaag gacggcgcgg cgaggcgcac aaggtgaccg tattgctggg catgaaagac 8821 ggcgtcatcc tggccgaacc cgagggtccc gaccagacgc ccgcctgatc ttcgaatgcc 8881 atcatcctgc cgggaagaat caactggcgg ctacgcctcc acggcgctgc ttcgctccat 8941 ccgcagaaaa cactcgtctt ccccgaccac tttcagcccc atccgcccat aaagcgcctg 9001 cgccggattg tccttgaaca ccgtcagccg cagcagccca cgccgctcgt catgcgccat 9061 cgcacgcacc tgttcgatgg tccaggcccc gacgccttgc ccgcgcgacg cctcgag //