LOCUS PK8CCDB 3024 bp DNA linear BCT 26-JUL-2016 DEFINITION Plasmid KIL18 (from E.coli) cytotoxic protein (ccdB) gene, complete cds. ACCESSION L27083 VERSION L27083.1 KEYWORDS ccdB gene; cytotoxic protein. SOURCE Plasmid pKIL18 ORGANISM Plasmid pKIL18 other sequences; plasmids. REFERENCE 1 (bases 1 to 3024) AUTHORS Bernard,P., Gabant,P., Bahassi,E.M. and Couturier,M. TITLE Positive-selection vectors using the F plasmid ccdB killer gene JOURNAL Gene 148 (1), 71-74 (1994) PUBMED 7926841 FEATURES Location/Qualifiers source 1..3024 /organism="Plasmid pKIL18" /mol_type="genomic DNA" /host="K-12" /db_xref="taxon:37150" /plasmid="Plasmid pKIL18" gene 1..381 /gene="ccdB" CDS 1..381 /gene="ccdB" /codon_start=1 /transl_table=11 /product="cytotoxic protein" /protein_id="AAA98268.1" /translation="MTMITNSSSVPGDPLESTCRHASLSLQFKVYTYKRESRYRLFVD VQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIG EEVADLSHRENDIKNAINLMFWGI" BASE COUNT 743 a 759 c 770 g 752 t ORIGIN 1 atgaccatga ttacgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg 61 catgcaagct tgtctttgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 121 ctgtttgtgg atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc 181 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 241 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 301 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 361 ctgatgttct ggggaatata aatgtcaggc tccgttataa agctagcttg gcactggccg 421 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 481 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 541 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc 601 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 661 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 721 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 781 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 841 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 901 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 961 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 1021 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 1081 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 1141 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 1201 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 1261 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 1321 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 1381 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 1441 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 1501 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1561 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1621 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1681 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1741 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1801 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1861 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1921 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1981 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 2041 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 2101 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 2161 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 2221 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2281 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2341 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2401 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2461 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2521 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2581 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2641 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2701 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2761 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2821 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 2881 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 2941 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 3001 aacaatttca cacaggaaac agct //