LOCUS       PSEDHD                  1792 bp    DNA     linear   BCT 28-SEP-1994
DEFINITION  Pseudomonas putida D-hydantoinase gene, complete cds.
ACCESSION   L24157
VERSION     L24157.1
KEYWORDS    D-hydantoinase.
SOURCE      Pseudomonas putida
  ORGANISM  Pseudomonas putida
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   LaPointe,G., Viau,S., LeBlanc,D., Robert,N. and Morin,A.
  TITLE     Cloning, sequencing, and expression in Escherichia coli of the
            D-hydantoinase gene from Pseudomonas putida and distribution of
            homologous genes in other microorganisms
  JOURNAL   Appl. Environ. Microbiol. 60 (3), 888-895 (1994)
   PUBMED   8161181
COMMENT     Original source text: Pseudomonas putida (library: DSM 84 genomic
            bank of A. Morin) DNA.
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Pseudomonas putida"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:303"
                     /clone_lib="DSM 84 genomic bank of A. Morin"
     CDS             245..1351
                     /EC_number="3.5.2.2"
                     /function="amidohydrolase"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hydantoinase"
                     /protein_id="AAA21752.1"
                     /translation="MSLLIRGATVVTHEESYPADVLCVDGLIRAIGPNLEPPTDCEIL
                     DGSGQYLMPGGIDPHTHMQLPFMGTVASEDFFSGTAAGLAGGTTSIIDFVIPNPQQSL
                     LEAFHTWRGWAQKSASDYGFHVAITWWSEQVAEEMGELVAKHWGEQLQALHGLQECNH
                     GRRRHPGGQLRALPATGCRAHRACPRTANWCTTCRKNCLPRGMTGPEAHPLSRPSQVE
                     GEAASRAIRIAETIGTPLYVVHISSREALDEITYARAKGQPVYGEVLPGHLLLDDSVY
                     RDPDWATAAGYVMSPPFRPREHQEALWRRLQSGQPAHHGHRPLLFLRRTESHGPRRLQ
                     SHPQQAPPASKTAWRCCGMPVSTAGDCRCMSSLR"
     regulatory      1701..1717
                     /regulatory_class="terminator"
BASE COUNT          387 a          589 c          512 g          304 t
ORIGIN      
        1 catcttgtca agtcagtcaa taacccctga aacccccgaa gtcagcaggt caataaaaat
       61 taagtgttaa atttcaaaca gttaaaaacc acaaaaataa taggaaaatt ttcttgaaga
      121 ttcccaaatc agcatctagc ctcgaatctt gtcaggtctg acaggattaa ccgccctgcc
      181 tgccctacac ccgagacaat tccaagaacc ggcccagacc ggtcagcctg cgaggaaaaa
      241 cggcatgtcc ctgttgatcc gtggcgccac cgtggtcacc cacgaagaga gttaccccgc
      301 cgatgtcctg tgtgtcgatg gcctgatccg tgccatcggg ccaaacctcg aaccgcccac
      361 cgactgtgaa atcctcgacg gcagcggcca gtacctgatg cccggcggca tcgacccgca
      421 tacccacatg cagttgccat tcatgggcac cgtggccagc gaggatttct tcagcggcac
      481 cgcagcgggc cttgccggcg gcaccacgtc gatcatcgac ttcgtcatcc ccaacccgca
      541 gcagtcattg ctggaggcct tccacacctg gcgcggctgg gcgcagaaga gcgccagcga
      601 ctacggcttc cacgttgcca tcacctggtg gagcgaacag gtggctgaag aaatgggcga
      661 actggtagcc aagcattggg gtgaacagct tcaagcactt catggcttac aagaatgcaa
      721 tcatggccgc cgacgacacc ctggtggcca gcttcgagcg ctgcctgcaa ctgggtgccg
      781 tgcccaccgt gcatgcccga gaacggcgaa ctggtgtacc acctgcagaa aaaactgctt
      841 gcccaggggc atgaccggac cagaggctca ccccctttcg cgcccttcac aagtggaagg
      901 tgaagcggcc agccgcgcca tccgtattgc cgaaaccatt ggtacgccgc tgtatgtggt
      961 gcacatttcc agccgtgaag cactggatga aatcacctat gcacgcgcca agggccagcc
     1021 ggtttacggc gaagtcttgc ccggccacct gctgctggac gacagcgtct accgtgaccc
     1081 ggactgggcc actgccgctg gctacgtgat gagcccgccg ttccgcccgc gcgagcacca
     1141 ggaggcgctg tggcgccgct tgcagtcggg ccaacctgca caccacggcc accgaccact
     1201 gctgtttctg cgccgaacag aaagccatgg gccgcgacga cttcagtcgc atccccaaca
     1261 ggcaccgccg gcatcgaaga ccgcatggcg gtgctgtggg atgccggtgt caacagcggg
     1321 tgactgtcga tgcatgagtt cgttgcgctg acctccacca acacggcaaa aatcttcaac
     1381 cttttcccac gcaagggcgc catccgcgtg ggttgccgac gccgacctgg tgctgtggga
     1441 cccgcagggc actcgcactc tatcggccca gacccaccac cagcgggtgg acttcaatat
     1501 ctttgaaggc cgcactgtgc gcggggtcac cccagccaca ccatcagcca gggcaaggtg
     1561 ctctgggccg atggcgacct gcgcgccgag gccggggcgg ggcggtatgt ggaacggccg
     1621 gcgtatccgt cggtgtacga ggtgctgggg cgacgcgccg aacagcagcg cccgacgccc
     1681 gttcagcgct gaggccattg gggctgctgc gcagcccatc gccggcaagc caaatataat
     1741 aaagagagag gctacaaccg tgatcgacgc cctgaaccac ttgccgcgcc cc
//