LOCUS ECOMUTL 3000 bp DNA linear BCT 27-JUL-1994 DEFINITION Escherichia coli N-acetylmuramoyl-L-alanine amidase (amiB) gene, complete cds, DNA repair protein (mutL) gene, partial cds, and two unidentified cds's. ACCESSION L19346 VERSION L19346.1 KEYWORDS DNA repair; DNA repair protein; N-acetylmuramoyl-L-alanine amidase; amiB gene; cell wall hydrolase; internal promoter; methyl-directed DNA repair; mutL gene; very-short-patch repair. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (bases 1 to 3000) AUTHORS Connolly,D.M. and Winkler,M.E. TITLE Genetic and physiological relationships among the miaA gene, 2-methylthio-N6-(delta 2-isopentenyl)-adenosine tRNA modification, and spontaneous mutagenesis in Escherichia coli K-12 JOURNAL J. Bacteriol. 171 (6), 3233-3246 (1989) PUBMED 2656644 REFERENCE 2 (bases 1 to 3000) AUTHORS Tsui,H.T., Mandavilli,B.S. and Winkler,M.E. TITLE Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium JOURNAL Nucleic Acids Res. 20 (9), 2379 (1992) PUBMED 1594459 REFERENCE 3 (bases 1 to 3000) AUTHORS Tsui,H.C., Zhao,G., Feng,G., Leung,H.C. and Winkler,M.E. TITLE The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase JOURNAL Mol. Microbiol. 11 (1), 189-202 (1994) PUBMED 7511774 COMMENT Original source text: Escherichia coli (strain K-12) (library: Clarke-Carbon) DNA. FEATURES Location/Qualifiers source 1..3000 /organism="Escherichia coli" /mol_type="unassigned DNA" /strain="K-12" /db_xref="taxon:562" /map="94.8 min" /tissue_lib="Clarke-Carbon" mRNA <1..>3000 /experiment="experimental evidence, no additional details recorded" /note="contiguous transcript in superoperon" CDS <1..1134 /function="unknown" /standard_name="urf1" /note="transcribed in vivo from the bacterial chromosome; ORF probably continues upstream; putative" /codon_start=1 /transl_table=11 /protein_id="AAA20095.1" /translation="GTGLRQAPRESISQLIDHANSHPAPIVAVDIPSGLLAETGATPG AVINADHTITFIALKPGLLTGKARDVTGQLHFDSLGLDSWLAGQETKIQRFSAEQLSH WLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAGAGLVRVLTRSENIAPLL TARPELMVHELTMDSLTESLEWADVVVIGPGLGQQEWGKKALQKVENFRKPMLWDADA LNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIESDRLHCAKRLVQRYGGVAVLKGA GTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGALLGQKLSPYDAACAGCVAHGAA ADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHDESSNSAP" mRNA (689.709)..>3000 /experiment="experimental evidence, no additional details recorded" /note="from putative Purf2-amiB internal promoter; unsure whether end results from an internal promoter or mRNA processing" CDS 1106..1567 /function="unknown" /standard_name="urf2" /experiment="experimental evidence, no additional details recorded" /note="possibly observed in minicells (Citation 3); transcribed in vivo from the bacterial chromosome; motif A nucleotide binding site encoded by bp 1208-1231" /codon_start=1 /transl_table=11 /protein_id="AAA20096.1" /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG" gene 1586..2923 /gene="amiB" CDS 1586..2923 /gene="amiB" /EC_number="3.5.1.28" /function="cell wall hydrolase" /experiment="experimental evidence, no additional details recorded" /note="gene product detected in minicells; transcribed in vivo; overexpressed AmiB has properties consistent with hydrolase function" /citation=[1] /citation=[2] /codon_start=1 /transl_table=11 /product="N-acetylmuramoyl-L-alanine amidase" /protein_id="AAA20097.1" /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN" sig_peptide 1586..1651 /gene="amiB" mRNA 2621..>3000 /experiment="experimental evidence, no additional details recorded" /note="from PmutL internal promoter; transcript start determined in vivo" gene 2933..>3000 /gene="mutL" CDS 2933..>3000 /gene="mutL" /function="methyl-directed and very-short-patch repair" /standard_name="MutL protein" /experiment="experimental evidence, no additional details recorded" /note="see EMBL sequence Z11831" /citation=[1] /citation=[2] /codon_start=1 /transl_table=11 /product="DNA repair protein" /protein_id="AAA20098.1" /translation="MPIQVLPPQLANQIAAGEVVERP" BASE COUNT 678 a 776 c 883 g 663 t ORIGIN 1 ggtaccggtt tgcggcaagc gccccgcgaa tccattagcc agttaatcga ccacgctaat 61 tcccatcctg cgccgattgt ggcggttgat atcccttccg gcctgctggc tgaaactggc 121 gctacgccag gcgcggtgat caacgccgat cacaccatca cttttattgc gctgaaacca 181 ggcttgctca ctggaaaagc gcgggatgtt accggacaac tgcattttga ctcactgggg 241 ctggatagtt ggctggcagg tcaggagacg aaaattcagc ggttttcagc agaacaactt 301 tctcactggc taaaaccgcg tcgcccgact tcgcataaag gcgatcacgg gcggctggta 361 attatcggtg gcgatcacgg cacggcgggg gctattcgta tgacggggga agcggcgctg 421 cgtgctggtg ctggtttagt ccgagtactg acccgcagtg aaaacattgc gccgctgctg 481 actgcacgac cggaattgat ggtgcatgaa ctgacgatgg actctcttac cgaaagcctg 541 gaatgggccg atgtggtggt gattggtccc ggtctgggcc agcaagagtg ggggaaaaaa 601 gcactgcaaa aagttgagaa ttttcgcaaa ccgatgttgt gggatgccga tgcattgaac 661 ctgctggcaa tcaatcccga taagcgtcac aatcgcgtga tcacgccgca tcctggcgag 721 gccgcacggt tgttaggctg ttccgtcgct gaaattgaaa gtgaccgctt acattgcgcc 781 aaacgtctgg tacaacgtta tggcggcgta gcggtgctga aaggtgccgg aaccgtggtc 841 gccgcccatc ctgacgcttt aggcattatt gatgccggaa atgcaggcat ggcgagcggc 901 ggcatgggcg atgtgctctc tggtattatt ggcgcattgc ttgggcaaaa actgtcgccg 961 tatgatgcag cctgtgcagg ctgtgtcgcg cacggtgcgg cagctgacgt actggcggcg 1021 cgttttggaa cgcgcgggat gctggcaacc gatctctttt ccacgctaca gcgtattgtt 1081 aacccggaag tgactgataa aaaccatgat gaatcgagta attccgctcc ctgatgagca 1141 ggcaacatta gacctgggcg agcgggtagc gaaagcctgc gatggcgcaa ccgtaatcta 1201 tctgtatggc gatttaggcg caggtaaaac cacctttagc cggggctttt tacaggctct 1261 gggtcatcag ggtaatgtca aaagccccac ttatacgctg gtcgaaccct atacgctcga 1321 caacttaatg gtctatcact ttgatttgta ccgccttgcc gatcccgagg agctggagtt 1381 tatggggatc cgcgattatt ttgccaacga tgccatctgc ctggtggagt ggccacaaca 1441 aggtacaggt gttcttcctg acccggatgt cgaaatacac attgattatc aggcacaagg 1501 ccgtgaggcg cgcgtgagtg cggtttcctc tgcgggtgaa ttgttgctgg cgcgtttagc 1561 cggttaacct ttgaaaggtg gcgggatgat gtatcgcatc agaaattggt tggtagcgac 1621 gctgctgctg ctgtgcacgc cggtgggtgc cgcgacgctc tctgatattc aggtttctaa 1681 cggtaatcaa caggcgcgga taacgttgag ttttattggc gatcctgatt atgcgtttag 1741 ccatcaaagc aaacgcaccg tggcgctcga tatcaaacaa acgggcgtga ttcagggact 1801 gccgttgttg ttcagcggca ataatctggt gaaggcgatt cgctctggaa cgcctaaaga 1861 tgcacaaacg ctacggctgg tggtcgatct tactgaaaac ggtaaaaccg aagcggtgaa 1921 gcggcagaat ggcagcaatt acactgtcgt ctttacgatt aacgccgatg tgccgccacc 1981 gcctcctccg ccgcccgtgg ttgcgaaacg cgttgaaacg cctgcggttg tcgcaccgcg 2041 cgtcagcgaa ccggcgcgca atccgtttaa aacggaaagt aaccgcacta cgggtgttat 2101 cagcagtaat acggtaacgc gtccggcagc gcgcgcgacg gctaacactg gcgataaaat 2161 tatcatcgct attgatgccg gacacggcgg tcaggaccct ggcgctatcg gccccggtgg 2221 tacgcgggag aaaaatgtca ccatcgccat cgcgcgtaaa ttgcgtactt tgctcaatga 2281 cgatccgatg ttcaaaggcg ttttaacccg tgacggggat tactttatct cggtgatggg 2341 gcgcagtgat gtggcacgta agcaaaacgc caatttcctc gtgtcgattc acgctgatgc 2401 cgcaccgaac cgcagtgcga ctggcgcttc cgtatgggtg ctctctaacc gtcgcgccaa 2461 cagtgaaatg gccagctggc tggagcagca cgagaaacag tcggagctgc tgggtggggc 2521 gggtgatgtg ctggcgaaca gtcagtctga cccctattta agccaggcgg tgctggattt 2581 acagttcggt cattcccagc gggtagggta tgatgtagcg accagtatga tcagtcagtt 2641 gcaacgcatt ggcgaaatac ataaacgtcg accagaacac gccagccttg gcgttctgcg 2701 ctcgccggat atcccatcag tactggtcga aaccggtttt atcagcaaca acagcgaaga 2761 acgtttgctg gcgagcgacg attaccaaca acagctggca gaagccattt acaaaggcct 2821 gcgcaattac ttccttgcgc atccgatgca atctgcgccg cagggtgcaa cggcacaaac 2881 tgccagtacg gtgacgacgc cagatcgcac gctgccaaac taaggacgat tgatgccaat 2941 tcaggtctta ccgccacaac tggcgaacca gattgccgca ggtgaggtgg tcgagcgacc //