LOCUS BACORFKLM 3033 bp DNA linear BCT 27-MAR-1995 DEFINITION Bacillus subtilis orfK, orfL and orfM, complete cds's. ACCESSION L16808 VERSION L16808.1 KEYWORDS . SOURCE Bacillus subtilis ORGANISM Bacillus subtilis Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus. REFERENCE 1 (bases 1 to 3033) AUTHORS Quirk,P.G., Guffanti,A.A., Clejan,S., Cheng,J. and Krulwich,T.A. TITLE Isolation of Tn917 insertional mutants of Bacillus subtilis that are resistant to the protonophore carbonyl cyanide m-chlorophenylhydrazone JOURNAL Biochim. Biophys. Acta 1186 (1-2), 27-34 (1994) PUBMED 8011666 COMMENT Original source text: Bacillus subtilis (individual_isolate MS11) (library: Tn917 insertional library no. 1) DNA. FEATURES Location/Qualifiers source 1..3033 /organism="Bacillus subtilis" /mol_type="genomic DNA" /isolate="MS11" /db_xref="taxon:1423" /tissue_lib="Tn917 insertional library no. 1" regulatory 51..57 /regulatory_class="ribosome_binding_site" /note="putative" CDS 69..1067 /note="Likely N-terminal signal sequence, followed by lipoamide anchoring site. No homologues in database.; putative; label: orfK" /codon_start=1 /transl_table=11 /protein_id="AAA64348.1" /translation="MKKGLIVLVAVIFLLAGCGANGASGDHKQLKEIRIGIQQSLSPL LIAKEKGWFEDAFEKEGIKVKWVEFQSGPPQFEGLAADKLDFSQVGNSPVIAGQAAGI DFKEIGLSQDGLKANGILVNQNSGIQDVKGLKGKKIAVAKGSSGFDFLYKALDQVGLS ANDVTIIQLQPDEAASAFENGSVDAWSIWEPYLSLETMKHGAKILVNGESTDLYSPGF TLVRTKFSEEHPDEVVRFLKVFNKAVVWQKEHLDEAADLYSDIKDLDKKVVENVLKNT EPLNEIISDDIVKAQQETADFQFRTKAIDKKIDVKDVVDNTFIKKALEEHSSGGDQ" regulatory 1052..1057 /regulatory_class="ribosome_binding_site" /note="putative" CDS 1064..1894 /note="protein is hydrophobic, with homology to E. coli ProW; putative; label: orfL" /codon_start=1 /transl_table=11 /protein_id="AAA64349.1" /translation="MMKAEAAGSLPKTNAEAVRKKPGRKRYGWMKGLLLPAVIIAIWQ VIGGLGVVSATVLPTPVTIVLTFKELILSGELFGHLQISIYRAALGFLLGAGLGLMIG ILAGFSKRTELYLDPSLQMLRTVPHLAVTPLFILWFGFDEVSKILLIALGAFFPVYIN TFNGIRGVDAKLFEVARVLEFKWHQQISKVILPAALPNILLGIRLSLGIAWLGLVVAE LMGSSSGVGYMIMDARQFSQTNKVFAGIIIFAVVGKLTDSFVRLLERKLLKWRNSYEG " regulatory 1904..1910 /regulatory_class="ribosome_binding_site" /note="putative" CDS 1917..3033 /note="Gene disrupted by Tn917 insertion after base 3033. Translation product hydrophilic, no homologues in the databases.; putative; label: orfM" /codon_start=1 /transl_table=11 /protein_id="AAA64350.1" /translation="MEILWFIPTHGDARYLGSESDGRTADHLYFKQVAQAADRLGYTG VLLPTGRSCEDPWLTASALAGETKDLKFLVAVRPGLMQPSLAARMTSTLDRISDGRLL INVVAGGDPYELAGDGLFISHDERYEATDEFLTVWRRLLQGETVSYEGKHIKVENSNL LFPPQQEPHPPIYFGGSSQAGIEAAAKHTDVYLTWGEPPEQVKEKIERVKKQAAKEGR SVRFGIRLHVIARETEQEAWEAAERLISHLDDDTIAKAQAALSRYDSSGQQRMAVLHQ GDRTKLEISPNLWAGIGLVRGGAGTALVGDPQTIADRIAEYQALGIESFIFSGYPHLE EAYYFAELVFPLLPFENDRTRKLQNKRGEAVGNTYFVK" BASE COUNT 841 a 610 c 842 g 740 t ORIGIN 1 gaattcgaga aaacagaaga tttggagtac gcagaaggat cagggatata gaaagggcgg 61 aagcggaaat gaaaaaagga ctcattgtat tggttgccgt tatttttctg ctcgcaggct 121 gtggagcaaa tggagccagc ggtgaccaca aacaattgaa ggaaattcgt attggtattc 181 agcaaagctt aagccccctt ttgattgcaa aagaaaaagg ctggttcgaa gatgcatttg 241 aaaaagaagg catcaaagtg aaatgggtgg agtttcaaag cggtccgccc caatttgaag 301 gccttgcggc agacaaactt gatttttctc aagtcggtaa ttcgcctgtg atcgctggac 361 aggcggcagg cattgacttt aaagaaatcg gtttgtctca ggacggcttg aaagcaaacg 421 gcatccttgt caatcaaaac agcggcattc aagacgttaa gggcttaaag gggaagaaaa 481 ttgcagtcgc caaaggcagc agcgggtttg attttttgta taaagcgctt gatcaagtgg 541 ggttgtcagc taacgatgtg acaattattc agcttcagcc tgatgaagca gcttccgcat 601 ttgaaaacgg atctgttgat gcctggtcca tttgggagcc gtacttgtcc ttagaaacaa 661 tgaaacacgg agcaaaaatc cttgtgaacg gagaaagcac agatttatat tcaccgggtt 721 ttactttagt aagaacaaag ttttcagaag agcatccaga cgaagtggtc cgttttctga 781 aggtgtttaa taaagccgtt gtatggcaaa aggaacatct ggatgaagca gcggacttat 841 attctgacat taaagatctc gataaaaagg tcgtcgaaaa tgtgctgaaa aatactgagc 901 cacttaatga gatcatcagt gacgacattg tgaaagcaca gcaggaaaca gcagattttc 961 aatttcgaac aaaggcgata gataagaaga ttgatgtgaa ggatgttgtc gataacactt 1021 ttatcaaaaa agcattagag gagcattcct caggaggtga tcaatgatga aagcagaggc 1081 tgcgggctcg ctgcccaaaa cgaatgcgga agcggtgcga aaaaagcccg gaaggaagag 1141 atacggctgg atgaaggggc ttttactgcc cgctgtcatc attgccatct ggcaggtcat 1201 cggcgggctg ggggttgtgt ccgctacagt gctgcctaca cctgttacga ttgttctgac 1261 ctttaaggag cttattttgt ccggcgaact gttcggccat ctgcaaatca gcatttatcg 1321 ggcggcactt ggttttttgc ttggcgcggg gcttggattg atgatcggga ttttggccgg 1381 attttccaaa cggacggagc tgtatcttga tccgtcgttg caaatgctta gaaccgtacc 1441 tcatctggcc gtcacgccgc tgtttatttt atggttcgga tttgacgaag tatctaaaat 1501 tttgctgatt gcgctcggtg ccttctttcc tgtctatatc aatacgttta acggcatccg 1561 cggggttgac gcgaagctgt ttgaagtcgc ccgggtgctt gaatttaaat ggcatcagca 1621 gatatccaaa gtgatattgc cggcagccct gcccaatata ttgctaggca ttcgcttatc 1681 gctcggcatt gcatggctcg ggctcgttgt ggcagagctg atgggctcaa gctcgggagt 1741 cggctatatg atcatggatg cgagacagtt ttcacaaacg aataaagtct ttgcagggat 1801 tatcattttt gcagtggttg gaaagctgac ggattccttc gtcaggctgc ttgaacgaaa 1861 gctgttaaag tggagaaaca gctacgaagg ataaaagata taaaggggag atgaatatgg 1921 aaattctttg gtttataccg actcatggag acgcgagata tttaggatct gaaagtgatg 1981 gacggactgc ggatcatctt tactttaaac aagtcgcgca ggcggcagac aggctgggat 2041 atacaggagt tcttctgcca acgggaagat cgtgcgagga cccgtggctt acagcgtcgg 2101 cactggcagg agaaacaaag gacttaaagt ttttagtcgc ggtaagacct ggtttaatgc 2161 agccttctct ggcagccaga atgacatcca ccctagaccg catttcagac ggccggctgc 2221 tgatcaacgt tgtggcaggg ggcgatccgt atgagctggc aggcgatggc ctgtttatca 2281 gccatgatga gcgctacgag gcaacagacg aatttttaac cgtttggcgg agacttttgc 2341 agggagaaac ggtttcctat gaaggaaagc atataaaagt ggaaaacagc aatctgcttt 2401 tcccgccgca gcaagagccg catccgccca tctatttcgg tggttcgtca caggctggaa 2461 ttgaagctgc cgcaaagcat acagacgtgt acttgacatg gggtgagccg cctgaacaag 2521 taaaagaaaa gatagaaaga gtcaaaaagc aagcggcaaa agaagggaga tccgttcgat 2581 ttggcattag gctgcatgtc attgcccgtg aaactgaaca agaggcatgg gaagcggcag 2641 agcgcttgat cagccacttg gatgatgaca ccattgccaa agcgcaggca gctttgagcc 2701 gttacgactc ttcaggccag cagcggatgg ccgtccttca tcaaggcgat cggacgaagc 2761 ttgaaatcag cccgaatctg tgggcgggga tcggcctggt cagaggaggg gcaggaacag 2821 ctcttgtcgg tgatccgcag acgattgccg accggatagc ggagtaccag gcactcggca 2881 tagaatcgtt tatattctcc ggctatccgc atttagaaga agcgtattat tttgcagagc 2941 ttgtatttcc gcttttgccg tttgaaaatg atcggacgag aaagcttcag aacaaaaggg 3001 gagaagcggt aggaaacact tattttgtca aga //