LOCUS       KX427128                 957 bp    DNA     linear   BCT 26-MAR-2017
DEFINITION  Mycolicibacterium neoaurum ATCC 25795 acyl-CoA dehydrogenase
            (fadE32) gene, complete cds.
ACCESSION   KX427128
VERSION     KX427128.1
KEYWORDS    .
SOURCE      Mycolicibacterium neoaurum ATCC 25795
  ORGANISM  Mycolicibacterium neoaurum ATCC 25795
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycolicibacterium.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Xu,L.-Q., Wang,F.-Q. and Wei,D.-Z.
  TITLE     Unraveling and Engineering of Acyl Coenzyme A Dehydrogenase to
            Produce Sitolactone in Sterol Metabolism in Mycobacterium neoaurum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 957)
  AUTHORS   Xu,L.-Q., Wang,F.-Q. and Wei,D.-Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2016) State Key Lab of Bioreactor Engineering,
            New World Biotechnology Institute, East China University of Science
            and Technology, No. 130, Meilong Road, Xuhui District, Shanghai
            200237, China
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Mycolicibacterium neoaurum ATCC 25795"
                     /mol_type="genomic DNA"
                     /strain="ATCC 25795"
                     /isolation_source="soil"
                     /culture_collection="ATCC:25795"
                     /type_material="type strain of Mycobacterium neoaurum"
                     /db_xref="taxon:1265312"
     gene            1..957
                     /gene="fadE32"
     CDS             1..957
                     /gene="fadE32"
                     /note="FadE32"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="ARA67647.1"
                     /translation="MNFEIDDQQRDFASSIDAALGAADVPSAIRAWADGDSAPARKVW
                     AQLTDLGVTALSVAEKFDGIEAQPIDLVVALERLGYWAVPGPVTESVAVAPILLADDE
                     RSAALAAGELIATVALPPQVPYAVNADFAGLTLLAGDGRVAEATPGSAHDSVDPTRRL
                     FEVDASGDGQSADTARAYEFGVLATAAQLVGAGQAMLDLSVAYAKQRTQFGRVIGSYQ
                     AIKHKLADVHIAVEMARPLVHGAALALADGSPDTARDVSAAKVAAADAALLAARSSLQ
                     THGAIGFTQEHDLSLLLLRVQALRSAWGDPTLHRRRLLEVLS"
BASE COUNT          140 a          326 c          327 g          164 t
ORIGIN      
        1 atgaacttcg agatagacga ccagcagcgt gatttcgcgt cgagcatcga tgccgcactc
       61 ggcgcggccg atgtcccgtc cgccatccgg gcctgggccg acggtgactc tgcccccgcc
      121 cgcaaggtat gggcacagct caccgacctc ggcgtgaccg ccctctcggt ggccgagaag
      181 ttcgacggga tcgaggctca accgatcgat ctggtggtgg cattggaacg gctgggttac
      241 tgggccgtcc cgggtccggt caccgaatct gttgccgtgg cacccatcct gctcgccgat
      301 gacgagcggt ccgcggcact ggctgccggg gagctgatcg ccaccgtggc acttccgcca
      361 caggtgccct atgccgtcaa cgccgacttc gcggggttga cgctgttggc cggagatggc
      421 cgggtggccg aggcgacacc gggttccgcg cacgattcgg tcgatcccac gcgacgcctc
      481 ttcgaggtcg acgcatccgg ggacggacag tccgccgata ccgcacgcgc ctacgagttc
      541 ggggtgttgg ccacggccgc ccagctggtc ggggcgggac aggcgatgct ggacctgtcg
      601 gtggcctatg ccaagcagcg cacccaattc ggccgggtga tcggctccta tcaggcgatc
      661 aagcacaagc tcgccgatgt gcacatcgcg gtcgagatgg cgcgcccgct ggtccacggg
      721 gcggcgttgg cgcttgccga cggctcaccg gacactgccc gcgatgtgag cgcggccaag
      781 gtggccgccg ccgatgccgc gctgttggca gcgcgttcgt cactgcagac ccacggcgcc
      841 atcggattca cccaggaaca cgacctgtcg ctgttgctcc ttcgagtgca ggcattgcgc
      901 tcggcctggg gtgacccgac cctgcaccgt cgccgcctgt tggaggtcct ttcgtga
//