LOCUS       KU697807                2142 bp    DNA     linear   BCT 03-MAY-2016
DEFINITION  Halomonas sp. Victoria JH oligoalginate lyase 2 gene, complete cds.
ACCESSION   KU697807
VERSION     KU697807.1
KEYWORDS    .
SOURCE      Halomonas sp. Victoria JH
  ORGANISM  Halomonas sp. Victoria JH
            Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
            Halomonadaceae; Halomonas.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Hobbs,J.K., Lee,S.M., Robb,M., Hof,F., Barr,C., Abe,K.T.,
            Hehemann,J.H., McLean,R., Abbott,D.W. and Boraston,A.B.
  TITLE     KdgF, the missing link in the microbial metabolism of uronate
            sugars from pectin and alginate
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 113 (22), 6188-6193 (2016)
   PUBMED   27185956
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Hehemann,J.-H. and Boraston,A.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2016) Biochemistry and Microbiology, University
            of Victoria, 3800 Finnerty Road, Victoria, BC V8P 5C2, Canada
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ABySS v. 1.3.4
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Halomonas sp. Victoria JH"
                     /mol_type="genomic DNA"
                     /strain="Victoria JH"
                     /isolation_source="intertidal zone near Victoria"
                     /host="Macrocystis sp."
                     /db_xref="taxon:1798866"
                     /geo_loc_name="Canada: British Columbia"
     CDS             1..2142
                     /note="OalBC-2; member of polysaccharide lyase family 17"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoalginate lyase 2"
                     /protein_id="ANB32496.1"
                     /translation="MSAKSEYLLWSSSELESLRQHCEAESSLGRSLKVLEQQVAASIA
                     RGVVVPGQGEAGSFEHNTHKENARLIEGAALLGRLNNDEAALAHARELLESYAACYRE
                     MPFQVARNTNPPGKLFHQILNEHIWLLHASLGLALLKPSLSDSQYRALLEGLFLPMLE
                     MFTETYRHDFDRIHNHGLWAVAAVGICAMVIESPEHLAISIEGLDGSGETGGFLAQIA
                     LLFSPQGYYVEGPYYHRFAIHPLCLFAEAIERQQPECNIFAHAEGRIGKSLHVLLSSA
                     YPDGRFPALNDASRTMNLDDEGARCAMALLAARYDVAPELLALARQQQGSWLHANGVA
                     LKRAVEGLGRDVQATSQRFTDGPEGNSGAHAHLKQGCGLQAQHVVLTAGQHGMGHGHF
                     DQLGLTYFARGKEVLEEYGFARWVNVETKFGGRYLKENEGYAKQSVAHNLVVVDMQSQ
                     HSGDHGLADAHHAELDLLAGSDAAACARCDNAYDGVAMQRTLVLLDSRHTQTPLLVDI
                     FSLSSAVPHVYDYTFNHGGQVVRWWDMESSPHPVSTALTTLEVLGESAGYQHLWKVAQ
                     RPAATAHCMTWLDGDSFHSWHQITQAPSEAYQLRVGANDPDHNLRPDAKVMTRLTATS
                     QTFYSLFESHGHFDESLEQCSGARPEVLNLHVLETCEGAAPGIDALEITFRSGRLLLV
                     LAEEGAVHAGRDITLGDHECHIRAGVNLFDL"
BASE COUNT          409 a          622 c          695 g          416 t
ORIGIN      
        1 atgtctgcaa agagtgaata tctgttgtgg tcgtccagcg agctagaaag tcttcgtcaa
       61 cactgcgagg cagagagctc actgggccgc agcctcaagg tgctggagca gcaggtggct
      121 gccagtatcg cgcgaggcgt ggtggtgccc ggccagggtg aggcgggaag cttcgagcac
      181 aatactcaca aggaaaatgc ccgcctgata gaaggcgcgg cgctgttggg gcgactcaat
      241 aatgatgagg cggccctggc gcatgccaga gagttgctgg aatcctacgc ggcctgctat
      301 cgcgagatgc cgtttcaggt cgcgcgcaat accaatcctc cgggcaagct gttccatcag
      361 atcctcaacg aacacatctg gctgctgcat gcgtcactcg gtcttgcatt gctcaagccg
      421 tcattgagcg atagccagta tcgggcgctg ctggagggct tgttcctgcc gatgctggaa
      481 atgttcaccg agacctatcg gcatgatttc gaccgcatcc acaaccacgg gctttgggcg
      541 gtggcggcag tcgggatctg cgcgatggtg atcgagagtc cagagcatct ggcaatctcc
      601 atcgaagggc tggatggcag tggcgagaca ggtggcttcc tcgcccagat agcattgctg
      661 ttctccccgc agggctatta cgtcgagggg ccgtattacc accgcttcgc gattcatccg
      721 ctgtgtctgt ttgccgaggc aatcgaacgc caacagcctg agtgcaacat cttcgcgcat
      781 gccgaggggc ggatcggcaa gtcgctgcat gtcctgctgt ccagcgccta tccggatggg
      841 cgcttcccgg cactgaatga tgcctcgcgc accatgaatc tggatgatga aggcgcccgc
      901 tgcgccatgg cgttgctggc ggcccgctac gatgtggccc cggaactgtt ggccctcgcg
      961 cgtcaacagc aaggcagctg gctgcatgcc aatggcgtgg cgctcaagcg tgcagtagag
     1021 gggctgggca gggatgtgca ggccaccagc cagcgcttca ctgatgggcc cgaaggcaac
     1081 agcggcgcgc atgctcacct caagcagggg tgtggattgc aggcccagca cgtagtgctc
     1141 accgcgggtc agcacgggat gggacatggt cactttgatc agctgggcct gacgtatttc
     1201 gcgcgtggca aggaagtgct tgaggagtat ggcttcgcgc gctgggtgaa tgtcgagacc
     1261 aagttcggcg gtcgctacct caaggaaaac gagggctacg ccaagcagag cgtcgcgcac
     1321 aacctggtgg tggtcgacat gcagtcccag catagcggtg accatgggct ggcggatgcg
     1381 caccacgcgg aactcgatct gctggcaggc agtgacgctg cggcgtgcgc ccgctgtgac
     1441 aacgcctacg acggtgtcgc catgcaacgc acgctcgtgt tgctggactc acgccatacg
     1501 cagacgccgt tgctggtgga tatcttctca ctgagctccg cagtgccgca tgtctatgac
     1561 tacaccttca atcacggtgg gcaggtggtg cgctggtggg acatggaatc gtctccgcat
     1621 cctgtctcca ccgccctgac gacgcttgag gtactgggcg aaagtgcggg ctatcaacac
     1681 ctgtggaagg tggcacagcg tccggcggca accgcgcact gcatgacctg gctggatggc
     1741 gacagcttcc atagctggca ccagatcacc caggcgcctt ctgaggccta tcaattgcgg
     1801 gtcggggcca atgatccgga tcacaacctg cggcctgacg ccaaggtgat gacgcgcctg
     1861 accgccacct ctcagacctt ctatagcctg ttcgaaagcc acggccactt cgatgaatct
     1921 ctcgagcaat gcagcggcgc gcgccctgaa gtgttgaatc tgcacgtgct ggaaacctgc
     1981 gaaggggcgg ccccaggtat cgatgcgctg gagatcacct tccgcagcgg ccggttgctg
     2041 ttggtgctgg ctgaggaggg ggccgtgcat gccgggcgcg acatcaccct gggggatcat
     2101 gaatgccaca tccgtgccgg ggtgaatctg tttgatctct ga
//