LOCUS       KK100223              106984 bp    DNA     linear   CON 26-FEB-2015
DEFINITION  Monoraphidium neglectum strain SAG 48.87 unplaced genomic scaffold
            scaffold00001, whole genome shotgun sequence.
ACCESSION   KK100223 AYTC01000000
VERSION     KK100223.1
DBLINK      BioProject: PRJNA221625
            BioSample: SAMN02981574
KEYWORDS    WGS.
SOURCE      Monoraphidium neglectum
  ORGANISM  Monoraphidium neglectum
            Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes;
            Chlorophyceae; CS clade; Sphaeropleales; Selenastraceae;
            Monoraphidium.
REFERENCE   1  (bases 1 to 106984)
  AUTHORS   Bogen,C., Al-Dilaimi,A., Albersmeier,A., Wichmann,J., Grundmann,M.,
            Rupp,O., Lauersen,K.J., Blifernez-Klassen,O., Kalinowski,J.,
            Goesmann,A., Mussgnug,J.H. and Kruse,O.
  TITLE     Reconstruction of the lipid metabolism for the microalga
            Monoraphidium neglectum from its genome sequence reveals
            characteristics suitable for biofuel production
  JOURNAL   BMC Genomics 14, 926 (2013)
   PUBMED   24373495
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106984)
  AUTHORS   Bogen,C.C., Al-Dilaimi,A.A., Albersmeier,A.A., Wichmann,J.J.,
            Grundmann,M.M., Rupp,O.O., Lauersen,K.K., Blifernez-Klassen,O.O.,
            Kalinowski,J.J., Goesmann,A.A., Mussgnug,J.J. and Kruse,O.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2013) Department of Biology/Center for
            Biotechnology, Bielefeld University, Universitaetsstrasse 27,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Newbler v. 2.8
            Assembly Name         :: mono_v1
            Genome Coverage       :: 49.3x
            Sequencing Technology :: Illumina GAIIx
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..106984
                     /organism="Monoraphidium neglectum"
                     /mol_type="genomic DNA"
                     /submitter_seqid="scaffold00001"
                     /strain="SAG 48.87"
                     /isolation_source="basin in greenhouse"
                     /type_material="reference material of Monoraphidium
                     neglectum"
                     /db_xref="taxon:145388"
                     /chromosome="Unknown"
                     /geo_loc_name="Germany: Koethen"
                     /collection_date="1980"
                     /note="habitat freshwater"
     gene            243..1377
                     /locus_tag="MNEG_0001"
     mRNA            join(243..275,444..602,874..1038,1285..1377)
                     /locus_tag="MNEG_0001"
                     /product="pyruvate,orthophosphate dikinase"
     CDS             join(243..275,444..602,874..1038,1285..1377)
                     /locus_tag="MNEG_0001"
                     /EC_number="2.7.9.1"
                     /codon_start=1
                     /product="pyruvate,orthophosphate dikinase"
                     /protein_id="KIZ07928.1"
                     /db_xref="InterPro:IPR015813"
                     /translation="MQKLGKEVPYKVGTMIEIPRGALRAGDLARTAEFFSFGTNDLTQ
                     TCMGISRDDAQAKFLAHYMKTGILAVDPFETLDTLGVGELVRIAVERGRKTRPDIELG
                     ICGEHGGDPASVAFFEEVGLDYVSCSPLRVPIARLAAAQAVLRAKKA"
     gene            2557..4996
                     /locus_tag="MNEG_0002"
     mRNA            join(2557..2607,2784..2809,2960..3062,3410..3577,
                     3852..3947,4138..4245,4491..4574,4838..4996)
                     /locus_tag="MNEG_0002"
                     /product="molecular chaperone GrpE"
     CDS             join(2557..2607,2784..2809,2960..3062,3410..3577,
                     3852..3947,4138..4245,4491..4574,4838..4996)
                     /locus_tag="MNEG_0002"
                     /EC_number="5.1.3.1"
                     /codon_start=1
                     /product="molecular chaperone GrpE"
                     /protein_id="KIZ07929.1"
                     /db_xref="InterPro:IPR000740"
                     /translation="MVSPRHPRTLLLRAAGEEEAATAEASTEEAGNGTAAAPAAPPLE
                     RARAALEADELDKATLEEALAELEAELSALRESAAAAEAEGARAVALEGSLSAAKDQY
                     LRLQADFDNFRRRTAKEKDELGSRAKGDVVVGLLPLVDNFELARTQVKTETDGEAKIQ
                     ASYQGLYKQMVEILRGLGVQPVNTVGSPFDPNFHEAIAREANDDVPDGTVLQEFRKGF
                     AMGDRLLRPAMVQVSFTDKPAEVPAAETQEEETPEQPAAQQQEGQQ"
     gene            complement(5544..5949)
                     /locus_tag="MNEG_0003"
     mRNA            complement(join(5544..5725,5850..5949))
                     /locus_tag="MNEG_0003"
                     /product="hypothetical protein"
     CDS             complement(join(5544..5725,5850..5949))
                     /locus_tag="MNEG_0003"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07930.1"
                     /translation="MTRSSPSHTPDPKPSLAYGGQKQLAAMDASTDGSGGNRDFQELA
                     AIIKKRRAEAAAAAEQAAAAAAAAQAEPEAPAVESAAADAPQAGAPSAE"
     gene            7345..8851
                     /locus_tag="MNEG_0004"
     mRNA            join(7345..7389,7493..7520,7745..7831,7980..8038,
                     8192..8307,8522..8619,8811..8851)
                     /locus_tag="MNEG_0004"
                     /product="ubiquitin-conjugating enzyme E2 N"
     CDS             join(7345..7389,7493..7520,7745..7831,7980..8038,
                     8192..8307,8522..8619,8811..8851)
                     /locus_tag="MNEG_0004"
                     /EC_number="6.3.2.19"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 N"
                     /protein_id="KIZ07931.1"
                     /translation="MAAAQAAQLPRRIIKETQRLLSEPAPGISASPSEDNLRYFDIMI
                     AGPSSSPYEGGAFKLELFLPEDYPMAPPKVRFLTKIYHPNVDKLGRICLDILKDKWSP
                     ALQIRTVLLSIQALMSAPNPDDPLAENVAKHWKDNEPEAIATAREWTRLYAHGPT"
     gene            11229..14402
                     /locus_tag="MNEG_0005"
     mRNA            join(11229..11675,12031..13450,13711..14402)
                     /locus_tag="MNEG_0005"
                     /product="hypothetical protein"
     CDS             join(11229..11675,12031..13450,13711..14402)
                     /locus_tag="MNEG_0005"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07932.1"
                     /translation="MRAHPQQQLQQQQQRGGSNGAIPRPSSGPAWGRAGAGVAAVSGA
                     PPPPPRSAPASAPVPVPAPPPPLGAAGGAPAAAGSPAVRSSPIDISGPLEARKEANRL
                     RMSMTGKKDRSSPYGTPKGGLKSPLTSPLVGDPMKVAALNLEPGVSKMPNEVLAEFGK
                     FKKSLEEQRRAEEMRAIKSTLTASLQRTSGDGGSGSGNSSAAGAPPAGAGAGEDGGVG
                     AAAGSGKPPQAPVAPAGAGGEAKKSSFKFNPNAKPFSLNVNAKPFVPGTSGGGSSGGS
                     GGGGLAPAAPAVRQPAVPIPAGNIGAPPGLGAPPGAMPGAGGHMGGAAGPPPPPPPGA
                     VVGMGGPPGVMHPGGAGGSLQGPPHMMLVPPPPGAMPGLQHGGMGGPGAMVPPGMGGL
                     PGHPGGRGPPPGAMGPPGALGPQGGPVAMMPLAVGLIPMGPAPGGPLAGGQGLPPGVM
                     AGPRPPGGMGGPGGGGGGGGAGKGDHPERRGGPGGPGGPGPHQGGLRPDPRGPPLDHH
                     GPPQRGPQGGDHHHHPGGGGAGSSGRGGPDMGVRGGGGGGGEGDGHHRSGAHHRESPR
                     VGKADGHGGGDGHYGGRDNGGGGPGGRGERERDGGYGGGMGGGPPASREGSFPSANGP
                     YSMPMSGGMMPTPMPVMMAPGGFMGAPGQMVGFSPGQQGPVMVRPMGPPGAMFLQMPV
                     GMPFVPVAPGTSPSGRAPFFPGGGGFIAVGPGPGAPMGMPGAPMGMPMGADGGPHGGP
                     GGHGPGGPSPGGGRGRGRGGRGGFERHGGGRGGVGPYHPSGPPPPSGGGERGSSSAAP
                     HGSPQQVPRDGSSQPAPPPSQPPPPPLEPAAVPAAPVAPAGGAAGGAGEGGAVE"
     gene            complement(15461..17669)
                     /locus_tag="MNEG_0006"
     mRNA            complement(join(15461..15696,15898..16219,16433..16748,
                     17029..17669))
                     /locus_tag="MNEG_0006"
                     /product="hypothetical protein"
     CDS             complement(join(15461..15696,15898..16219,16433..16748,
                     17029..17669))
                     /locus_tag="MNEG_0006"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07933.1"
                     /translation="MATQDPEAGRLAEQVAALRWRCLHAPADDAPHDDSPAGARTGST
                     AAASAAAAIIALPPPPLLPPQQPIQREPPSFGEQLFALERRWGALRKLLEAPPDIGVP
                     GIGRGAAASPAGLLRGLLSELFRDVEALWLDAGREPAAYSLMEAVADAAMEFETEAAV
                     AAAARAAGVPAGGGGARGGALETASAFGVARAAALAGADGARRVILSDDLWQQWDTFA
                     ARTRAQEILSQELEQAAFAAAAALEASVLDAATDACAPASPMTQATVPLRAAVPAATS
                     HLVSADLPLPLIGAASAAVDTVAGVAVLGAASSQAIAFVGNKQAGSSSACSSSSDFTA
                     AAHAPHAESSAQGRLDDGKAITAAPMSRRQRQREHRRLGQQQQQQSAAAIPDAAAATA
                     APHTAGQAVTMQQQQQQQQQQPQRPCRHGAKRRRAANAAGAQLEAAARAIAGWRLQGV
                     AKPRARVARGELQRPRDSSLRGTLLRAILRVARAAEVVTAGTIAGAALSHAFLA"
     gene            complement(18925..24613)
                     /locus_tag="MNEG_0007"
     mRNA            complement(join(18925..19068,19242..19393,19571..19660,
                     19881..19978,20231..20379,20600..20704,20936..21075,
                     21211..21277,21477..21601,21802..22146,22345..22528,
                     22738..22989,23217..23327,23531..23632,23921..24098,
                     24377..24425,24592..24613))
                     /locus_tag="MNEG_0007"
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     CDS             complement(join(18925..19068,19242..19393,19571..19660,
                     19881..19978,20231..20379,20600..20704,20936..21075,
                     21211..21277,21477..21601,21802..22146,22345..22528,
                     22738..22989,23217..23327,23531..23632,23921..24098,
                     24377..24425,24592..24613))
                     /locus_tag="MNEG_0007"
                     /EC_number="2.1.1.14"
                     /codon_start=1
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="KIZ07934.1"
                     /db_xref="InterPro:IPR002629"
                     /translation="MAVSTTIGFPRTGSKRQAKVALESFWKGKTSEPDLVASFAAVDE
                     EAWRAQQAAGVQLIALDGTYYDQVLDMVYALGVAPARFKHLSGLELYFAMARGAPNAP
                     ALDMSKLLDTNYHYLVPELEEGFAPKPDFSAILDRLKRGQALLGKDAAVPMMVGPVTF
                     ALLSSTTLPIESVVEKLLPSYAQLLRQLQAVGAPEVQLHEPALATDKGAAAQRAAQAA
                     YAQLAAVGCPIHLVACYDDLGEAYPWVAQLPVRALTLDFCGVPGATAPNATLDLIRAN
                     GWPADKRLGVGCVDGRSVWADDEGACTALLNELRTLGASSLAVTSSVSLQHLPWDVTL
                     EPALPAGLAPRLAFAVQKLGAIARLAAGKGVSGAAGAAGGWVGRVRDSAMELERAMFD
                     RAENFEARRAKQPQFPAFPTTSIGSFPQTAQVRRLRAQLKSGAIDRPTYERLIDQQIA
                     LAIGIQEGLDIDVLVHGEAERTDMSEHFGMQLSGMVFTQHGWVQSYGSRYVRPPIIAG
                     DVAFVNAMTVREFKVAQDLTPRPVKGMLTGPVTILNWSFPRKDLSRRAQAFQLALALR
                     QEVAALQDAGCRIVQVDEPALREGLPLKHAKWDSYLSWAVDAFRLCTAVAAPSTQVVT
                     HLCYSDFGDIMKPIIDMDADVLTIENSRSGDAMVRALAQAGYTADLGPGVYDVHSPVV
                     PGVDALVAKLRTFLATGILAGAPERLWVNPDCGLKTRTWDEVVPSLRNMVAAAHALRA
                     EVEGPSKGPGKGVAATTAAPARSCAAAGCC"
     gene            complement(32577..33782)
                     /locus_tag="MNEG_0008"
     mRNA            complement(join(32577..32726,32998..33090,33354..33419,
                     33525..33613,33737..33782))
                     /locus_tag="MNEG_0008"
                     /product="50S ribosomal protein L18"
     CDS             complement(join(32577..32726,32998..33090,33354..33419,
                     33525..33613,33737..33782))
                     /locus_tag="MNEG_0008"
                     /codon_start=1
                     /product="50S ribosomal protein L18"
                     /protein_id="KIZ07935.1"
                     /db_xref="InterPro:IPR005484"
                     /translation="MFTSMPTVARAAPSTGRAFTPVVCKAGQDSRKARTAKRHQSIRK
                     KVSGTTERPRLAVFRSNQHIYAQVIDDTRSITLAAASTLTPDVKAAVEGNGGTVSAAE
                     AVGKKVAELAQAAGISKVAFDRGGFAYHGRIQALADGARGAGLQF"
     gene            34135..36200
                     /locus_tag="MNEG_0009"
     mRNA            join(34135..34221,34378..34533,34734..36200)
                     /locus_tag="MNEG_0009"
                     /product="Alpha-1,2 glucosyltransferase ALG10"
     CDS             join(34135..34221,34378..34533,34734..36200)
                     /locus_tag="MNEG_0009"
                     /EC_number="2.4.1.-"
                     /codon_start=1
                     /product="Alpha-1,2 glucosyltransferase ALG10"
                     /protein_id="KIZ07936.1"
                     /db_xref="InterPro:IPR007006"
                     /translation="MSAAWSVTVLSLLLAVVAVVNKLAPEAYMDEPFHVPATQRYCAG
                     DFTTWDPKITTFPGLYVVGAAYAWLARGLAGWAGIRMEVACSTAYLRSLNAILALACL
                     QLAHAVSYELQRRCWGRTAVGRRRQRLQDEVQQGGQQHNNQQHAAHHQQQQQQQQQQQ
                     QQRGAQQPSNADEVAMRRSALVLALVVSLLPTHAFFAFLYYTDVASMAALLLTQLLLL
                     RGMVRGGALAAAVAVGMRQTNAVWVTLMIGGAVLEDLLEGDGQGQGGAQQQQQQQQQG
                     GQTAAKEGYYGGEAIGGGAADATPQDLSLIEELSAALPRTWRFRQQLLERYGPLLLVP
                     AAFAAFVVWNGGVTVGDREAHAPVKHLAQPLYFGLFALAAAGPLLLAPAAGGGAPALL
                     AAVRRRPVLEAAGTLVAVAAAALAVRRGTLAHPYLLADNRHYTFYLWRRVMARHWAAR
                     YALIPAELLGWRLLLGGLAARQRRLWCAAYAAAVVVTLLPAWLVEPRYFTPGLVLGLL
                     HLWSPGTAAAAGGGARAAARWNACALLATGLGYAAVNAAVLYMFLARPFSWPDGSVAR
                     FMW"
     gene            complement(36905..39429)
                     /locus_tag="MNEG_0010"
     mRNA            complement(join(36905..37219,37502..38457,38713..39033,
                     39231..39429))
                     /locus_tag="MNEG_0010"
                     /product="hypothetical protein"
     CDS             complement(join(36905..37219,37502..38457,38713..39033,
                     39231..39429))
                     /locus_tag="MNEG_0010"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07937.1"
                     /translation="MARRLALSVLVLALFAGAAMAQDCSGITGCLKCGMRGKKEGRDD
                     KVWCTVCDEANGYALKAKKGRCGCAAGYYSRTAEGDKLGACKKATGTTVAAAGPINDG
                     ANIARECLDNSIPDVTHGHCECKAGYYVLLGGQTPRCIQCTPGSYTDAPNLRLACKLC
                     SVGKIHNAAHTACINLSEAFSGVADVITGVQAKAAEVVAGAGAATQKSVADARAAAQD
                     ALDGLAERLDASGLPEGAQRGIATVAEHLKPAIAQLSAHAQGALNNVTATLKNATGLD
                     LPSAFDDLDIQDRLTSLGNSALAQVNSTLQTAIKAAPAALKTVVAAPVTANAAAPADA
                     KAVPSAVVPDVHAPIMAANATHNVTGRPWPVIARAVDLSSVPGFELADPRLAERDADD
                     VDEIVEEGGTPGDDSEPLEPSDHLVSSKHHLAPSEHPAPSEHPAPSAHATFVDALVKR
                     LNLTQPFEKIKAKMQKEGDENQPSMSDYLGEVPSVGHILNMLRGGLPPIEDIPGLEQG
                     STGPMDTMVKLLRNVGGEDSRPREGLEEVMPSIMHGVMSNPLLSMRQRMGSTGQDQDE
                     QGDQDSAAPKVRPAGQAHVPRSHITYEYED"
     gene            complement(40289..41765)
                     /locus_tag="MNEG_0011"
     mRNA            complement(join(40289..40687,40897..41036,41179..41375,
                     41605..41765))
                     /locus_tag="MNEG_0011"
                     /product="hypothetical protein"
     CDS             complement(join(40289..40687,40897..41036,41179..41375,
                     41605..41765))
                     /locus_tag="MNEG_0011"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07938.1"
                     /translation="MKAKSKKIARPVTVWMDRSAPRTPSYESMRASVRAQGAKFHANS
                     QAYEEGKKSKYTGLWCRKTEWTTQWSCCLREPGRGGPDHSWSPDDEPLLLLQCNSEVE
                     AALTHDLGALWLTLNATGGATEEQLKRMRWNLPQLQLWQDGAVKSLSGIHLEDKGRAR
                     TTIRRLAQRLLRQLPAGMLPPAQAKKIAKDDKAKAVKKERQRKAAAKMREADKKRFAK
                     VAFLVHQASLTARKTQKQEAAKRRAAEERAKAAKKRAAEREAVARIKKVRAVAEAERA
                     HERLKRERDARVKQRATKRART"
     gene            complement(46174..48982)
                     /locus_tag="MNEG_0012"
     mRNA            complement(join(46174..46293,46559..46780,46950..47099,
                     47302..47505,47717..47892,48964..48982))
                     /locus_tag="MNEG_0012"
                     /product="hypothetical protein"
     CDS             complement(join(46174..46293,46559..46780,46950..47099,
                     47302..47505,47717..47892,48964..48982))
                     /locus_tag="MNEG_0012"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07939.1"
                     /translation="MAQCARAEHSSSEGWLVLDRFHFPDGSTPVEVTFGCGMRETGEI
                     YRQAADGILGMGNNANALHSQLARAGITSRVFSLCFGFPAGGTLLLGDVGLPPSVGPL
                     SFTPLLASPTPYYVVRLEQLSIGGASLNLEPAIFAKGYGTVLDSGTTFSYLPTAAFAA
                     FTAALDAAIVGRGMRKGSGPDPAYPDVCYSGAPEKFEDAAKMFPTASLRFAGGAVLSL
                     PPHRYLFLMGRGEYCLGVFDNGQSGTLIGGIAVRNALVLYDLERQRIGFAEADCPNLG
                     SAVAAAAAAEPVSPGCRDTH"
     assembly_gap    48619..48797
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(49674..51147)
                     /locus_tag="MNEG_0013"
     mRNA            complement(join(49674..50276,50479..51147))
                     /locus_tag="MNEG_0013"
                     /product="hypothetical protein"
     CDS             complement(join(49674..50276,50479..51147))
                     /locus_tag="MNEG_0013"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07940.1"
                     /translation="MDQWHSLRDAALGRFDGGYGEGSVTIAKRLAHFAGGGFLKTSWE
                     AEAARQARMARVNTEEVEDVPKMTAGDVGAAGVGVGGEAAGAISSDTLHISSGSRSPQ
                     DGRSSEQEQQEQEQQQDASVGNLLDERVGRGSGGAAQAAAAGGEEEWGQDDEGFDGDA
                     HAPAALVPVTSMLALAMWAVAVAQWWPVVPEAWYGLTNGLADVTLWQLATTYPDTPVT
                     WALQMDTTAVAVDGEWHRLLTAGLLHSGLLPLVCCADGVRNAGSWVEAYCGSLQAVVT
                     VVLCAAGAGLGQLLAGRADTGLAGAGVCTGLYAAWLTLGLTRGRGVMPITSDRGVLYA
                     LLNALMAAYQPAVGFACLAGGALGGVAGTLLAPLLARVLYTCTAVTAAVALSLARAAL
                     ETVRLGLGVVLALLVATFRAAGEVARTVRGL"
     gene            52082..54728
                     /locus_tag="MNEG_0014"
     mRNA            join(52082..52095,52270..52395,52598..52677,52911..52981,
                     53197..53283,53393..53531,53706..53811,54096..54199,
                     54424..54560,54678..54728)
                     /locus_tag="MNEG_0014"
                     /product="hypothetical protein"
     CDS             join(52082..52095,52270..52395,52598..52677,52911..52981,
                     53197..53283,53393..53531,53706..53811,54096..54199,
                     54424..54560,54678..54728)
                     /locus_tag="MNEG_0014"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07941.1"
                     /translation="MQSARISSPAADWITDNLGDGYVVREKFLGGSGWSSTYIYTTEA
                     GAEYFVKTSGGAKAEGMFKGEALGLKAMFDTNTVRIPDVHHVGLLPDARGAFIVMEYL
                     RLEGGGSQAELGRQMALMHLAEPTDPNAKAGKFGFAVDNTIGATPQPNEWMDDWVEFY
                     RERRLRHQLDLLGDARMKALAQPVLDNLGFWFTDIAGSIRPSILHGDLWSGNVSSSGG
                     RPVIFDPATYYGHAEAEWGMSWCAGFSGAFWQGYFEVAPKAPLFDQRRDLYTLYHILN
                     HANLFGGGYNSQAQTILQRLNEQLKRSQ"
     gene            complement(57571..58381)
                     /locus_tag="MNEG_0015"
     mRNA            complement(join(57571..57827,58036..58381))
                     /locus_tag="MNEG_0015"
                     /product="hypothetical protein"
     CDS             complement(join(57571..57827,58036..58381))
                     /locus_tag="MNEG_0015"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07942.1"
                     /translation="MSDTRSMPAVLPFSVDTWTTETASKAQFLTHAHKDHTVGISQNA
                     RHMYCTATTLHLLRIKFPELAAAIDRGDVQVTVLRDPGDSAIGDTLHLWSAGACGGYP
                     YSVTPLFLANHCAGAVMLLFESEVWGSILHTGDCRVTSRQLPDLAAALDELLGEGPHL
                     GLLCLDVTAGGGQDGADTLVCGWAQGSCAVIMLTGALPQL"
     gene            59620..65096
                     /locus_tag="MNEG_0016"
     mRNA            join(59620..59654,59995..60077,60270..60979,61274..61569,
                     61767..63311,63493..63899,64169..64382,64580..65096)
                     /locus_tag="MNEG_0016"
                     /product="hypothetical protein"
     CDS             join(59620..59654,59995..60077,60270..60979,61274..61569,
                     61767..63311,63493..63899,64169..64382,64580..65096)
                     /locus_tag="MNEG_0016"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07943.1"
                     /translation="MLLCENDKSALSDELRQRDTKGDFPFYCTSCSEAGFHVPEAPVG
                     AGRSRKRRHARRGSGSGDDEDDEDYQGQEGGEEEGEQGLNAEQQHEDEEGEDEPDAGE
                     VMEDVAPVARQPQSDRAESKPSHHRVAAPPHSSAAAPAASGRGGARSAARGRGRGRGA
                     GRSVGGEAVVRGDRLDRWAARRAAEERKLRRGTEAARAGHTGPALGTAVADEIVTPLR
                     PNGASGGGGSGGAPAAARRDNLAATRGRPLSAGSVKLWEGTLELVSARDVVEGVKLRK
                     PIVVSKARIDVGPDCNRRALAPFEAALRRHAAIEPSGAAGAAGVTRGAAQGGRRLTAY
                     VGKAVAVGEAFARELGRTEDFDVTAFKVTFPAASSVKEGLMAFGCGASTHWVVLPLEG
                     VPHPLLFTVRQHDLGLMCMHPKDAATAARVFRQLSRYGDAAPWAPALPTNAADDGYQA
                     QTAGAQQRLQQAQQHAQQLAQQGPGGMVQEGSRQMAWDESNAHDAARRAATHPVSWAW
                     EPASPASPGPATSDRTGAGREGRGDSLMTAQEEAGSEEAASDAAGAEEAGAGGYVGGS
                     STDSDSTGSTERLEGLARGRRRGGPHEVVELVESEDGAQGGQWQHGSGGVLRQQRGQG
                     ALPDEAGGTAEDGDEAEGVAPTQALDGDAMELDEEGWGEQQEQQEQHEQQEQRQQPSV
                     SSDIEAEDAAPSGSPACAAPQLDSLMALFNTRSAGEQEPGRHGGQADSLHKGAEGSAR
                     AHEHARPAALGVQSPDGTLLASHAARTPGMGRHGRTAANIVQQQQQQQQQQQQQQANA
                     SRATGLAPLSIERGPGESPGYQRAVESSGVQQLLDSGVAAGPGGLALSRLMPGVETLW
                     QSGGGGWRPFRVLLLGFGGGSDAHDDALTDGLEECRRLGAHVFCSLPDLRAAVRLQRA
                     THGPGTPTVLVLPRRLGPAGIRHALEAAAAPLGRGGGEADGPDQQAAAPLPPWWQVLA
                     TLAQPALVFYEAPGEGEGLMTLARLWRGDGRPPPSEDRPSWAAALARCQLHACGISLV
                     ACEVALQHATPASMARLLSLLARCRSLARAAPADFHLAVRRCDLDNLGHRNAAVAGVL
                     AAREHADAVLLLEDLLGEGDPEAGHQAGDGDVDSPGHPATLPSSRGDVLPRHLATASL
                     GLEGGEPDVVQDAVHLAWLRRRQLRLVVVLVPDAADVDDSRHHQTSAGCPDSNGRAHS
                     GDGSELGGDQRRAVSHEHAEVALAALRAVPRHGSVLAGSWDQTLRLLVSIADSTEQEQ
                     QQQQQH"
     gene            66323..68874
                     /locus_tag="MNEG_0017"
     mRNA            join(66323..66365,66486..66550,66757..66831,66960..67226,
                     67427..67501,67842..68027,68159..68192,68340..68478,
                     68715..68874)
                     /locus_tag="MNEG_0017"
                     /product="hypothetical protein"
     CDS             join(66323..66365,66486..66550,66757..66831,66960..67226,
                     67427..67501,67842..68027,68159..68192,68340..68478,
                     68715..68874)
                     /locus_tag="MNEG_0017"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07944.1"
                     /translation="MGKHSRSPSPSGSDSSEHRHKHKRHKQKKEKHKKDKSKKQKRAS
                     PDRDKERLEAARAFLQQVLKQGGGGDAAAAAAAAVFAAEKQVANAATAADGGSGDKAS
                     DKAEQKRQRRRQERPPKPKPPGVGDITAADYFSRAAEFTAWLQEDRVLYFNELGGDEA
                     RSLFVEFVEDWNGGHGLVLAPTKRTTHEWGFTAKGGGGRGGSMSALMADQQQQRHEGQ
                     LAAREAEGREKRKWRSDQKDLLDEMLPKATGREALVEKRMARREAAKAREDSPEMMRV
                     TGGGDVMGGDDSFAAAKAREASYNARRNQRDLAKREQLTQKLSAAQEAEAAKMAQFRA
                     LLAHGPISIPKRQ"
     gene            complement(71613..73544)
                     /locus_tag="MNEG_0018"
     mRNA            complement(join(71613..72512,72809..73119,73322..73544))
                     /locus_tag="MNEG_0018"
                     /product="hypothetical protein"
     CDS             complement(join(71613..72512,72809..73119,73322..73544))
                     /locus_tag="MNEG_0018"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07945.1"
                     /translation="MTWNEGHSWTLDIPLPAGKVTFKVVMQEANGGVRWETSTGNRAV
                     TLPATTDAATPVGSVGVACSWGEISDTRVKALPDRSFLRSRLDALEGRASALRHQQSR
                     TVLLSGPTHSAPSVPDGEVGLAGTAWTPGAAVPSGVGERALGATVDQLLQVAHEALDR
                     GDSSAGAIALGAAECEAEEPTSLSRCCILLAVSQAATAALENQVNLAAAWAASKTQAS
                     LLADEPAAPVGPAMPQLRDSVFSDEAPPATTSPQTAAGQAGKDPAGPAAALVPTPEDQ
                     GGALMFSADSLRAEVDPHVMLRAVLERTTRSAAGATSAPAAAAAAYLETELRSTPTEV
                     EAPPPEVQHSSAPQTAEVPAAGRVATVAAASNVVLSVDAQSSTGTAVARPTQVVPAPS
                     GAGPQRQQLQSLLGGIEAKADACRTLTAQIAQGRAAKRDAALEVAAAPTVATIASLND
                     GLFAARASALAVVVAMLMSSAGRRMGA"
     gene            75899..77678
                     /locus_tag="MNEG_0019"
     mRNA            join(75899..76040,76285..76378,76656..76855,77056..77159,
                     77352..77558,77673..77678)
                     /locus_tag="MNEG_0019"
                     /product="hypothetical protein"
     CDS             join(75899..76040,76285..76378,76656..76855,77056..77159,
                     77352..77558,77673..77678)
                     /locus_tag="MNEG_0019"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07946.1"
                     /translation="MLGWLGGSIALVVFCGVTLWCSFMLSDMYEVDGRKHGTYGDAVI
                     SVLGRGSAVAVTVCQLSNLVLSSIGYSVAAGESMKMVVHSHCDVRDTGCGSTVWQMSI
                     VFGITQLFFSQMPTLESAWWSSMVGAAMSVLYSTAALGMGAASVGRKLEPRVKAFGVF
                     NALGAIAFAYSFSAVLLEVQDTIHEPPKSKLTMRRAVGASMAVTFVFYVGVGFVGYAA
                     LGDATPGNILTGFNSPKALVTAANAMVLVHMK"
     gene            complement(79067..80245)
                     /locus_tag="MNEG_0020"
     mRNA            complement(join(79067..79153,79376..79720,79901..79990,
                     80201..80245))
                     /locus_tag="MNEG_0020"
                     /product="Carotene biosynthesis-related protein CBR"
     CDS             complement(join(79067..79153,79376..79720,79901..79990,
                     80201..80245))
                     /locus_tag="MNEG_0020"
                     /codon_start=1
                     /product="Carotene biosynthesis-related protein CBR"
                     /protein_id="KIZ07947.1"
                     /translation="MAALIARPLALKALKANRAQKCSGRRIAVCRAENKPQETINYGG
                     QSYTPAEWEAAKASGNLPTAEPVPVAAASTPAPVGFSSLMAFSGPAPELINGRLAMLG
                     VVAAIAAELASGESVLKQVSDEPTGIVFAFGIFIAASFAPLLSNVLPSAESVGPFNAK
                     AELINGRAAMIGFAALLAIELAKGSALF"
     gene            complement(80641..81890)
                     /locus_tag="MNEG_0021"
     mRNA            complement(join(80641..80705,80857..81022,81189..81295,
                     81522..81591,81801..81890))
                     /locus_tag="MNEG_0021"
                     /product="diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase"
     CDS             complement(join(80641..80705,80857..81022,81189..81295,
                     81522..81591,81801..81890))
                     /locus_tag="MNEG_0021"
                     /EC_number="1.1.1.193"
                     /codon_start=1
                     /product="diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase"
                     /protein_id="KIZ07948.1"
                     /db_xref="InterPro:IPR004794"
                     /translation="MNRALDLAMLAFGKTHPNPHVGCVIVRDGQVVGEGYHPKAGQPH
                     AEVWALRGAGQAAQGATAYVTLEPCNHYGRTPPCSQALVEAGVKRVVVGAGDPNPLVA
                     AAGIATLQSAGIEVALMDGAENQRAKDINTEFLARMEEEAAIEMARLQALAKSREAAG
                     AQHSD"
     gene            complement(<98719..99006)
                     /locus_tag="MNEG_0022"
     mRNA            complement(<98719..99006)
                     /locus_tag="MNEG_0022"
                     /product="hypothetical protein"
     CDS             complement(<98719..99006)
                     /locus_tag="MNEG_0022"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07949.1"
                     /translation="MAAAAAAHALAGTHRYRTTDIESDASSFEDLLLPKPLVHALAEA
                     GFQRPSPVQKVAIPLGMVGTDLIVQAKSGTGKTCVFAVIALQRIKSDVTTPQ"
     gene            complement(101113..103211)
                     /locus_tag="MNEG_0023"
     mRNA            complement(join(101113..101289,101455..101794,
                     102145..102353,102603..102848,103092..103211))
                     /locus_tag="MNEG_0023"
                     /product="hypothetical protein"
     CDS             complement(join(101113..101289,101455..101794,
                     102145..102353,102603..102848,103092..103211))
                     /locus_tag="MNEG_0023"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07950.1"
                     /translation="MIGLIGVMGGAAALEIRRLRHHRGQDDKDGQVKELKKTVEQYAK
                     ELEQERRVAAAAAERANALAAEADGLRRDTDHLRGAAESLGRNKEELAQQNAQLLLTN
                     LKLKDESQQLVEANRLLTEKMTELSAEREAEAAHFAAELAALRDRVAGVLQRMFSSEI
                     DEGAAVEALRELGCEVTFVPDTTARTEQSHTSASQRAMQATLNISGASSTSGASTPRF
                     TLSWLGGAPESTGLGTSSANAAVQQQGVEQQLVKIAQAKAASDVPRGGGDNCKDENVT
                     SGGSLGKSVNRSNGCVEQGGNLVFASQAKGTSTKSRLGLSLTDASGIFGSSKKGASVS
                     SGRFDQAMVAGQEDTGSDLSGSKDLVARK"
     gene            complement(104408..105316)
                     /locus_tag="MNEG_0024"
     mRNA            complement(join(104408..104540,104797..104990,
                     105138..105181,105286..105316))
                     /locus_tag="MNEG_0024"
                     /product="hypothetical protein"
     CDS             complement(join(104408..104540,104797..104990,
                     105138..105181,105286..105316))
                     /locus_tag="MNEG_0024"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="KIZ07951.1"
                     /translation="MLAQKTAHCTGVAKRPGPLHGARPRLFSFGAKKSKEAPTKRPTV
                     VPSPSFNVPLGLLVISGLSAYEGVTPLAGIAGLLGVFLAIQATRVKFVFDDVGLEVVI
                     AGKEEEETENKFVGGRNRWSYDSFINWVGNL"
CONTIG      join(AYTC01000001.1:1..48618,gap(179),AYTC01000002.1:1..58187)
//