LOCUS KJ896830 2220 bp DNA linear SYN 19-MAR-2015
DEFINITION Synthetic construct Homo sapiens clone ccsbBroadEn_06224 FSHR gene,
encodes complete protein.
ACCESSION KJ896830
VERSION KJ896830.1
KEYWORDS ORFeome Collaboration; Gateway cloning system; full-length ORF
without stop codon.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 2220)
AUTHORS Yang,X., Boehm,J.S., Yang,X., Salehi-Ashtiani,K., Hao,T., Shen,Y.,
Lubonja,R., Thomas,S.R., Alkan,O., Bhimdi,T., Green,T.M.,
Johannessen,C.M., Silver,S.J., Nguyen,C., Murray,R.R.,
Hieronymus,H., Balcha,D., Fan,C., Lin,C., Ghamsari,L., Vidal,M.,
Hahn,W.C., Hill,D.E. and Root,D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8 (8), 659-661 (2011)
PUBMED 21706014
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 2220)
AUTHORS Root,D.
TITLE Direct Submission
JOURNAL Submitted (28-MAY-2014) Broad Institute, 75 Ames St., Cambridge, MA
02142, USA
COMMENT This clone is distributed via members of the ORFeome Collaboration
(http://www.orfeomecollaboration.org/), including the
Dana-Farber/Harvard Cancer Center (DF/HCC) DNA Resource Core DNA
Repository
(http://www.dfhcc.harvard.edu/core-facilities/dnaresource/) and the
DNASU Plasmid Repository at ASU Biodesign Institute
(http://dnasu.asu.edu/DNASU/Home.jsp). See also
http://www.broadinstitute.org/rnai/public/ for additional
information on this clone.
FEATURES Location/Qualifiers
source 1..2220
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
/clone="ccsbBroadEn_06224"
/focus
/note="derived from Homo sapiens; Vector:pDONR223; derived
from source materials corresponding to GenBank accession:
BC118548.1; indicated CDS derived from human cDNA clone"
source 69..2154
/organism="Homo sapiens"
/mol_type="other DNA"
/db_xref="taxon:9606"
CDS 69..>2154
/note="missing stop codon"
/codon_start=1
/transl_table=11
/product="FSHR"
/protein_id="AIC54403.1"
/translation="MALLLVSLLAFLSLGSGCHHRICHCSNRVFLCQESKVTEIPSDL
PRNAIELRFVLTKLRVIQKGAFSGFGDLEKIEISQNDVLEVIEADVFSNLPKLHEIRI
EKANNLLYINPEAFQNLPNLQYLLISNTGIKHLPDVHKIHSLQKVLLDIQDNINIHTI
ERNSFVGLSFESVILWLNKNGIQEIHNCAFNGTQLDELNLSDNNNLEELPNDVFHGAS
GPVILDISRTRIHSLPSYGLENLKKLRARSTYNLKKLPTLEKLVALMEASLTYPSHCC
AFANWRRQISELHPICNKSILRQEVDYMTQTRGQRSSLAEDNESSYSRGFDMTYTEFD
YDLCNEVVDVTCSPKPDAFNPCEDIMGYNILRVLIWFISILAITGNIIVLVILTTSQY
KLTVPRFLMCNLAFADLCIGIYLLLIASVDIHTKSQYHNYAIDWQTGAGCDAAGFFTV
FASELSVYTLTAITLERWHTITHAMQLDCKVQLRHAASVMVMGWIFAFAAALFPIFGI
SSYMKVSICLPMDIDSPLSQLYVMSLLVLNVLAFVVICGCYIHIYLTVRNPNIVSSSS
DTRIAKRMAMLIFTDFLCMAPISFFAISASLKVPLITVSKAKILLVLFHPINSCANPF
LYAIFTKNFRRDFFILLSKCGCYEMQAQIYRTETSSTVHNTHPRNGHCSSAPRVTNGS
TYILVPLSHLAQN"
BASE COUNT 588 a 573 c 442 g 617 t
ORIGIN
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61 ttggcaccat ggccctgctc ctggtctctt tgctggcatt cctgagcttg ggctcaggat
121 gtcatcatcg gatctgtcac tgctctaaca gggtttttct ctgccaagag agcaaggtga
181 cagagattcc ttctgacctc ccgaggaatg ccattgaact gaggtttgtc ctcaccaagc
241 ttcgagtcat ccaaaaaggt gcattttcag gatttgggga cctggagaaa atagagatct
301 ctcagaatga tgtcttggag gtgatagagg cagatgtgtt ctccaacctt cccaaattac
361 atgaaattag aattgaaaag gccaacaacc tgctctacat caaccctgag gccttccaga
421 accttcccaa ccttcaatat ctgttaatat ccaacacagg tattaagcac cttccagatg
481 ttcacaagat tcattctctc caaaaagttt tacttgacat tcaagataac ataaacatcc
541 acacaattga aagaaattct ttcgtggggc tgagctttga aagtgtgatt ctatggctga
601 ataagaatgg gattcaagaa atacacaact gtgcattcaa tggaacccaa ctagatgagc
661 tgaatctaag cgataataat aatttagaag aattgcctaa tgatgttttc cacggagcct
721 ctggaccagt cattctagat atttcaagaa caaggatcca ttccctgcct agctatggct
781 tagaaaatct taagaagctg agggccaggt cgacttacaa cttaaaaaag ctgcctactc
841 tggaaaagct tgtcgccctc atggaagcca gcctcaccta tcccagccat tgctgtgcct
901 ttgcaaactg gagacggcaa atctctgagc ttcatccaat ttgcaacaaa tctattttaa
961 ggcaagaagt tgattatatg actcagacta ggggtcagag atcctctctg gcagaagaca
1021 atgagtccag ctacagcaga ggatttgaca tgacgtacac tgagtttgac tatgacttat
1081 gcaatgaagt ggttgacgtg acctgctccc ctaagccaga tgcattcaac ccatgtgaag
1141 atatcatggg gtacaacatc ctcagagtcc tgatatggtt tatcagcatc ctggccatca
1201 ctgggaacat catagtgcta gtgatcctaa ctaccagcca atataaactc acagtcccca
1261 ggttccttat gtgcaacctg gcctttgctg atctctgcat tggaatctac ctgctgctca
1321 ttgcatcagt tgatatccat accaagagcc aatatcacaa ctatgccatt gactggcaaa
1381 ctggggcagg ctgtgatgct gctggctttt tcactgtctt tgccagtgag ctgtcagtct
1441 acactctgac agctatcacc ttggaaagat ggcataccat cacgcatgcc atgcagctgg
1501 actgcaaggt gcagctccgc catgctgcca gtgtcatggt gatgggctgg atttttgctt
1561 ttgcagctgc cctctttccc atctttggca tcagcagcta catgaaggtg agcatctgcc
1621 tgcccatgga tattgacagc cctttgtcac agctgtatgt catgtccctc cttgtgctca
1681 atgtcctggc ctttgtggtc atctgtggct gctatatcca catctacctc acagtgcgga
1741 accccaacat cgtgtcctcc tctagtgaca ccaggatcgc caagcgcatg gccatgctca
1801 tcttcactga cttcctctgc atggcaccca tttctttctt tgccatttct gcctccctca
1861 aggtgcccct catcactgtg tccaaagcaa agattctgct ggttctgttt caccccatca
1921 actcctgtgc caaccccttc ctctatgcca tctttaccaa aaactttcgc agagatttct
1981 tcattctgct gagcaagtgt ggctgctatg aaatgcaagc ccaaatttat aggacagaaa
2041 cttcatccac tgtccacaac acccatccaa ggaatggcca ctgctcttca gctcccagag
2101 tcaccaatgg ttccacttac atacttgtcc ctctaagtca tttagcccaa aacttgccaa
2161 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
//