LOCUS       KI669098              405147 bp    DNA     linear   CON 23-MAR-2015
DEFINITION  Necator americanus unplaced genomic scaffold
            N_americanus-1.0_Cont85, whole genome shotgun sequence.
ACCESSION   KI669098 ANCG01000000
VERSION     KI669098.1
DBLINK      BioProject: PRJNA72135
            BioSample: SAMN02953824
KEYWORDS    WGS; HIGH_QUALITY_DRAFT.
SOURCE      Necator americanus (New World hookworm)
  ORGANISM  Necator americanus
            Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
            Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae;
            Bunostominae; Necator.
REFERENCE   1  (bases 1 to 405147)
  AUTHORS   Mitreva,M.
  TITLE     Draft genome of the hookworm Necator americanus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 405147)
  AUTHORS   Mitreva,M., Abubucker,S., Martin,J., Minx,P., Warren,C.,
            Pepin,K.H., Palsikar,V.B., Zhang,X.W.E. and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2013) The Genome Institute, Washington University
            School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA
COMMENT     Necator americanus is a roundworm that causes most of the human
            hookworm infections. N. americanus is a blood-feeding nematode
            infecting people in the rural areas of the tropics and subtropics
            causing an estimated disease burden of 22 million disability
            adjusted life years. The life cycle of the parasite begins when
            parasite eggs from an infected host are passed in the feces. In
            favorable environmental condition they hatch, and through 2 larval
            stages develop to the infective stage (L3). L3s penetrate human
            skin, migrate through the circulatory system and lung to finally
            reside in duodenum. The development continues and the adult stages
            attach to the intestinal mucosa and feed on blood.
            
            The strain being sequenced was obtained from the laboratory of Dr.
            Peter Hotez by Dr. Bin Zhan, originally isolated from an infected
            patient in Hunan province, China, and has been maintained in
            hamster since 1976.The adult worms were collected from intestines
            of hamsters infected subcutaneously with N. americanus L3 for 8
            weeks.  Worm isolation and DNA extraction was performed by Bin Zhan
            with QIAamp DNA mini kit according to manufacturers instructions
            (Qiagen). Jian et. al., 2003 Exp Parasitol.
            
            This assembly consists of fragments, 3kb and 8kb insert whole
            genome shotgun libraries. The sequences were generating on the
            Roch/454 platform and assembled using Newbler. To improve
            scaffolding, an in-house tool CIGA (Cdna tool for Improving Genome
            Assembly), was used to map 454 cDNA reads using blat to the genomic
            assembly to link genomic contigs based on cDNA evidence. Only joins
            confirmed by additional independent data typing were accepted.
            
            The repeat library was generated using Repeatmodeler (A.F.A. Smit,
            R. Hubley & P. Green http://repeatmasker.org). The Ribosomal RNA
            genes were identified using RNAmmer (Lagesen et. al., 2007 Nucleic
            Acids Res.) and transfer RNA's were identified with tRNAscan-SE
            (Lowe and Eddy, Nucleic Acids Res. 1997). Non-coding RNAs, such as
            microRNAs, were identified by sequence homology search of the Rfam
            database (Griffiths-Jones et. al., 2003 Nucleic Acids Res.).
            Repeats and predicted RNA's were then masked using RepeatMasker (A.
            Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding
            genes were predicted using a combination of ab initio programs Snap
            (Korf, 2004 BCM Bioinformatics), Fgenesh (Salamov A., Solovyev V.
            2000, Genome Res.) and Augustus (M. Stanke, et. al., 2008
            Bioinformatics) and the annotation pipeline tool Maker (M. Yandell
            et. al., 2007 Genomic Research) which aligns mRNA, EST and protein
            information from same species or cross-species to aid in gene
            structure determination and modifications. A consensus gene set
            from the above prediction algorithms was generated, using a
            logical, hierarchical approach developed at the Genome institute.
            Gene product naming was determined by BER
            (http://ber.sourceforge.net).
            
            Our goal is to explore this WGS draft sequence of N. americanus to
            better define proteins involved in nematode parasitism that impact
            health and disease and are relevant to both host-parasite
            relationships and basic biological processes.
            
            For information regarding this assembly or project, or any other
            GSC genome project, please visit our Genome Groups web page
            (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person. For specific questions regarding the N.
            americanus genome project contact Makedonka Mitreva
            (mmitreva@genome.wustl.edu) at Washington University School of
            Medicine. The National Human Genome Research Institute (NHGRI) of
            the National Institutes of Health (NIH) provided funds for this
            project.
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: High-Quality Draft
            Current Finishing Status :: High-Quality Draft
            Assembly Method          :: Newbler v.
                                        MapAsmResearch-04/19/2010-patch-
                                        08/17/2010
            Assembly Name            :: N_ americanus_v1
            Genome Coverage          :: 26.15x
            Sequencing Technology    :: 454
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..405147
                     /organism="Necator americanus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="N_americanus-1.0_Cont85"
                     /host="Homo sapiens"
                     /db_xref="taxon:51031"
                     /chromosome="Unknown"
                     /lab_host="hamster"
                     /geo_loc_name="China: Hunan Province"
     gene            complement(7231..9477)
                     /locus_tag="NECAME_05211"
     mRNA            complement(join(7231..7363,8053..8219,8288..8392,
                     9409..9477))
                     /locus_tag="NECAME_05211"
                     /product="hypothetical protein"
     CDS             complement(join(7231..7363,8053..8219,8288..8392,
                     9409..9477))
                     /locus_tag="NECAME_05211"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69498.1"
                     /translation="MATADQKPPANLGADRKRSSIVKGSFQSDTALYGGKTGVKQILT
                     APSQSHSRMTPGGQTFLCVMGSVISVAISIWLAAFSREPGWRRAVFATGAALCAVMFA
                     FLAFMTIRRAKHPPEPEALPDLSQRRSTIGARVSKRSIAAAGLASTGGDDCPTQV"
     gene            <13517..24571
                     /locus_tag="NECAME_05212"
     mRNA            join(<13517..13681,23187..23274,24402..24571)
                     /locus_tag="NECAME_05212"
                     /product="hypothetical protein"
     CDS             join(<13517..13681,23187..23274,24402..24571)
                     /locus_tag="NECAME_05212"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69499.1"
                     /translation="DGDAENSDETSDLSNIEGLIEHCANLVEAKYLMQHLFNACLEHA
                     VAAAKADSLNKESEARIQQLEQQSYINEQLLSTVIEDKTLNEINIDVVAVDLNVFILF
                     RDDRLVAAGVVCRAVICMLNPVVRMNLVFLSAKCEDFP"
     assembly_gap    25207..26039
                     /estimated_length=833
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            34954..35864
                     /locus_tag="NECAME_05213"
     mRNA            join(34954..35044,35460..35561,35653..35864)
                     /locus_tag="NECAME_05213"
                     /product="hypothetical protein"
     CDS             join(34954..35044,35460..35561,35653..35864)
                     /locus_tag="NECAME_05213"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69500.1"
                     /translation="MASAGVNSSKGMNWANINLDSLSVHVRSFYVNDVEGMLEQNALK
                     KSRSPSLASLRNGSVSPNHTDGSDCAPLPYRARRHTATADELLYPTEVVEPAATAPKE
                     TLKAESGGDEEKKEKNTGCAFEFLRRVGSYHV"
     gene            37632..40228
                     /locus_tag="NECAME_05214"
     mRNA            join(37632..37801,38335..38480,38695..38825,39181..39250,
                     39306..39489,39545..39651,39763..39869,39936..40028,
                     40091..40228)
                     /locus_tag="NECAME_05214"
                     /product="WD domain, G-beta repeat protein"
     CDS             join(37632..37801,38335..38480,38695..38825,39181..39250,
                     39306..39489,39545..39651,39763..39869,39936..40028,
                     40091..40228)
                     /locus_tag="NECAME_05214"
                     /inference="protein motif:HMMPfam:IPR019781"
                     /codon_start=1
                     /product="WD domain, G-beta repeat protein"
                     /protein_id="ETN69501.1"
                     /db_xref="InterPro:IPR019781"
                     /translation="MLSTSRLPSLAEVSARDEVPAIPRERAGADPTRASKSVFARLVP
                     WSTEASPSTLLNRRQGRIVPVRPGTGGTQYRVVSRTATVEGHSKAVLSVAATEDLLIS
                     GSKDRTAKLWDLQTGSEVRTLGVHPNNVHAVRFIPNSQLAISVSMYQIRVWDLRTQDC
                     LRVLQSSGQVNDGDGGPAGSRQNTVPFLETVVNAAEVDPSGRLLFTSFGGDVRIWNLE
                     KWASFGRLIGAVNSPRSEVSCLAVSEGADGLPQIFTGSRDHYVKMFQTSVGGEGVYEA
                     MVDFTPPHYDNVTCVVPFGGYVLSASKDKNIMKFALTDYKRDHLELNAHNSFIQGMCL
                     IGEHTERPLLCSVCKEGTMKFWDITTTRRFKIVEEVNFGFTRSVLQI"
     assembly_gap    41306..41606
                     /estimated_length=301
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    42472..43665
                     /estimated_length=1194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(44237..59875)
                     /locus_tag="NECAME_05215"
     mRNA            complement(join(44237..44440,46878..47027,47105..47225,
                     47407..47504,48067..48138,48210..48302,48360..48534,
                     48637..48694,48789..48962,50496..50631,50687..50844,
                     50904..51057,52674..52838,52912..53014,53074..53189,
                     53470..53595,53657..53806,55371..55505,55587..55706,
                     55762..55827,55912..56058,56733..56808,59799..59875))
                     /locus_tag="NECAME_05215"
                     /product="kinesin motor domain protein"
     CDS             complement(join(44237..44440,46878..47027,47105..47225,
                     47407..47504,48067..48138,48210..48302,48360..48534,
                     48637..48694,48789..48962,50496..50631,50687..50844,
                     50904..51057,52674..52838,52912..53014,53074..53189,
                     53470..53595,53657..53806,55371..55505,55587..55706,
                     55762..55827,55912..56058,56733..56808,59799..59875))
                     /locus_tag="NECAME_05215"
                     /inference="protein motif:HMMPfam:IPR001752"
                     /note="KEGG: cel:T01G1.1 klp-12; Kinesin-Like Protein
                     family member (klp-12); K10395 kinesin family member
                     4/7/21/27 7.5e-258"
                     /codon_start=1
                     /product="kinesin motor domain protein"
                     /protein_id="ETN69502.1"
                     /db_xref="InterPro:IPR001752"
                     /translation="MDGSSSSSMLSSRGGDVPSTVQVALRIRPQGNREKLEGSRVCTS
                     VIPGEPQVTIGTDRSFTYDHVFDQATKQSEIYDSCIEKLVNGLFDGYNATVLAYGQTG
                     SGKTYTMGTAFDAGAISEHEVGVIPRALAHVFRRVIELKREAQENGMLEPTFDVSVQF
                     IELYNEEIIDLLANDRASSVNVRIHEDAQGEIYLHGVTSKAVQDLHSTLEILKNGALN
                     RTVAATNMNEQSSRSHAIFSLHIKQQRVAVAADGPSPSQAIEMELLSAKFHFVDLAGS
                     ERLKRTGATGDRAKEGISINCGLLALGNVISALGGASGKVSHVPYRDSKLTRLLQDSL
                     GGNSRTLMIACVSPSDSDFVETLNTMKYANRAKNIKNKVVANQDKSSKLIGELRGRIA
                     ALEAELLEFKQGRRTIDGDGIETVNDQYHENVMLTAEINQLRFRVKALQETNEILRNR
                     NVDLMAKATGPNGLISSQTNDENGASSDDANDSSSNDAVMDPVQATLRKYLDELERTR
                     SQLYESQAVSNQLRKEMTKWKNQALLGGSGFPASADHSFSPFGHQQLIDEARADIERL
                     RKTVSVASNEANIKSSAVYSYIIATSDYASHVDTTEEDGTSNDADELDEDEDYDQDVD
                     DEQVFEDDNKGAVLRDNLATLQTEINIKERLIAELEQSDRRLAEVRLTYEKKLTELSA
                     QIKKMEAERDKVLMEAEAKKTDKASQEQAKRIRDEYERKLSDMRNEFRKLQMVEREHK
                     RMQARQERERQELIRYQGELKELKRVKVDLMKKIKDEMKRAQQQQNSNAKRLAAMDKE
                     ARRRDNLIRQLENKDRQREQFMKRTNEEIIRLRKAQKEQARQTRSAASQRITPMRTTV
                     SRMARTPVNRGQPPPEVAFSPKQAKMKWTFIEKRLSRLVTQKQTVGKMEEELNRQLEE
                     RTRLMDEINNLVSSGNLDYLLFSQLFAKISPTFSYSLTRFRRF"
     assembly_gap    54128..55007
                     /estimated_length=880
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    58733..58832
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    73162..73261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <85561..92185
                     /locus_tag="NECAME_05216"
     mRNA            join(<85561..85674,86126..86292,91561..91685,91760..91826,
                     91915..92185)
                     /locus_tag="NECAME_05216"
                     /product="leucine Rich repeat-containing domain protein"
     CDS             join(<85561..85674,86126..86292,91561..91685,91760..91826,
                     91915..92185)
                     /locus_tag="NECAME_05216"
                     /codon_start=1
                     /product="leucine Rich repeat-containing domain protein"
                     /protein_id="ETN69503.1"
                     /translation="LNTTNLDSLKRPEIVRTLTIRCPHFSKKKSTPAAGLFRGLHKLY
                     SLIIKNARLPTIPNDLFAHTPNLMTLDMSGNELRIEPYSLKSLRSVFELHNLIHLDLS
                     NNSINFLANTLISLTKLNVLTMDHNNSRTSIFAELSDLSLRHNFITTLHYAPPSVSYR
                     FLRNLRRIDLSGNMLEFISGSGSVNMLPAGLKQADLSNNRISFVQDGALSLMEKLALL
                     DLKYVGSLFESRHFFISIIITQFLTITKR"
     assembly_gap    87900..87999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    89021..89120
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <96482..100618
                     /locus_tag="NECAME_05217"
     mRNA            join(<96482..96607,97882..98025,99682..99905,
                     99964..100024,100354..100433,100543..100618)
                     /locus_tag="NECAME_05217"
                     /product="hypothetical protein"
     CDS             join(<96482..96607,97882..98025,99682..99905,
                     99964..100024,100354..100433,100543..100618)
                     /locus_tag="NECAME_05217"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69504.1"
                     /translation="NAQNIAICEKLRFDHLSALPESITELRIDTADWNQWDVGKLQNT
                     LSSLKSLTHLQLSSTSITKVPSQMGHLSQLLLAGNPLLQLSAENLKKRSDSTKKAITT
                     RSYEETLRPDSIIDVNDRNSNEMSPSSPVPDQPLSSSQIAYRSRSLTGRYVTSTVSST
                     NSTTQLTITTPRQRRKYQRDNNPDKFLNALIFVLFACVLILVVAIAFTLYFRFIRIDS
                     SRKPQKRLPEAMPLNVSA"
     assembly_gap    101391..101490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    106587..106852
                     /estimated_length=266
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    109159..109934
                     /estimated_length=776
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(116316..118599)
                     /locus_tag="NECAME_05218"
     mRNA            complement(join(116316..116438,116498..116623,
                     116681..116827,116990..117145,117895..118131,
                     118196..118294,118513..118599))
                     /locus_tag="NECAME_05218"
                     /product="hypothetical protein"
     CDS             complement(join(116316..116438,116498..116623,
                     116681..116827,116990..117145,117895..118131,
                     118196..118294,118513..118599))
                     /locus_tag="NECAME_05218"
                     /inference="protein motif:HMMPfam:IPR008465"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69505.1"
                     /db_xref="InterPro:IPR008465"
                     /translation="MTIPESTFKDVEDGDTFKLRLSVQPLGKKESWVFYDHKKVKGVP
                     IEEGEYEFRLEARDKAGQMASAPFKVTVTPSFPFNHIVSLELDTPFLRLSKPSSLSAF
                     VHQLALAFRSKPDAITIRNITGNATKSLVTWSNNTIPHKTCAHPALDSVRYTMLTRQR
                     SQTKIEFVKQVGSQFHVRKASMDLSGSCLDKEVEPVSTAPTVEAADQAAFPWTVFFGV
                     LILLLIVAVLILAICSAMRKNKKKEKPSDYMGKGMPVVFPEEVAEDVEMAHAATPMLT
                     KEERPPLKVSQHENPLYKPPPPLAAASPRVGTAAAVPNQRMPPTYVPP"
     assembly_gap    121585..122601
                     /estimated_length=1017
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<122656..124289)
                     /locus_tag="NECAME_05219"
     mRNA            complement(join(<122656..122724,122798..122917,
                     123969..124079,124194..124289))
                     /locus_tag="NECAME_05219"
                     /product="hypothetical protein"
     CDS             complement(join(<122656..122724,122798..122917,
                     123969..124079,124194..124289))
                     /locus_tag="NECAME_05219"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69506.1"
                     /translation="MWLLLLLPVFVTAAYRETVYVGQVYQRPLSQKTVATGATGGNLP
                     RWLIVRDGTLQGIPRSEDVGRHTVKISTSTRTQALLELIVKEDTRNPCGSEDTYWVEA
                     LYAEDGPVEDRFDAALDVADALKVNLSELK"
     assembly_gap    134612..134711
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(147354..148160)
                     /locus_tag="NECAME_05220"
     mRNA            complement(join(147354..147531,148126..148160))
                     /locus_tag="NECAME_05220"
                     /product="hypothetical protein"
     CDS             complement(join(147354..147531,148126..148160))
                     /locus_tag="NECAME_05220"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69507.1"
                     /translation="MMPIRFLSARQIRTAPKLCRKEFASARSRSAMESTETLPVVAFS
                     YRRLFLTLGRDIIKSFIMLPSRFIFA"
     assembly_gap    151353..151452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            161638..>167705
                     /locus_tag="NECAME_05221"
     mRNA            join(161638..161778,162086..162211,162263..162394,
                     162457..162555,162755..162853,163570..163689,
                     163816..163856,164300..164415,165159..165231,
                     165479..165574,165629..165744,165853..165943,
                     166034..166157,166224..166313,166998..167138,
                     167208..167302,167414..167480,167550..>167705)
                     /locus_tag="NECAME_05221"
                     /product="MOZ/SAS family protein"
     CDS             join(161638..161778,162086..162211,162263..162394,
                     162457..162555,162755..162853,163570..163689,
                     163816..163856,164300..164415,165159..165231,
                     165479..165574,165629..165744,165853..165943,
                     166034..166157,166224..166313,166998..167138,
                     167208..167302,167414..167480,167550..>167705)
                     /locus_tag="NECAME_05221"
                     /inference="protein motif:HMMPfam:IPR002717"
                     /inference="protein motif:HMMPfam:IPR006628"
                     /note="KEGG: bmy:Bm1_24465 histone acetyltransferase;
                     K11304 histone acetyltransferase HTATIP 1.2e-134"
                     /codon_start=1
                     /product="MOZ/SAS family protein"
                     /protein_id="ETN69508.1"
                     /db_xref="InterPro:IPR002717"
                     /db_xref="InterPro:IPR006628"
                     /translation="MSDGSVERAGSRRNEEELASKQLNIQYKRYYVDVKQNNRGRFIK
                     IAEMGSNYKSRLVLTMTAAVQLCEQLSEMIKFSETLPEGAEATAENGALKSEVLAFDS
                     RRYYLDLKENQRGRFLRIAQTVANPRMSRAQIAIPAQGMIEMRDTLHEMLEKYSEGYL
                     NDGNSTDASKTKQLTADNNKTFYFDVGKNDRGTFVRVTEVKQPTGYRTSITIPQSALD
                     KFRHLLDDVIEDLAAPAAKAQRYSVLSATRFISRWMVVLTTDNHPGGENTIPGLDRIV
                     EGCRLMVKMTTQNEYRDAEVLLVRSDPSRFYVHYIDCNRRLDEWVGPENLNLDSLRMP
                     QKGKKGGVMQSVESTSASGSPEREMSKKSAAMRKRKAATMDEDSQDGIGQNGAPSTRG
                     SMSLVGHSEDALTRIRNIEMIELGRHRIQPWYFAPYPQQLVHLPCIYICEFCLKYVKS
                     QTCLKTHMKKCYLKHPPGNEIYRNEPLSFFEIDGRKNKAYAQNLCLLAKLFLDHKTLY
                     YDTDPFLFYVLAFLDDRGFHIVGFFSKEKESAEEYNVACILVLPPYQKMGYGRLLIEF
                     SYELSKVEGKTGSPEKPLSDLGLLSYRSFWSATIIEKLMRFKEEEITAGEERAISVMD
                     LSQMTSIRKEDVISTLQ"
     assembly_gap    165318..165417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    175624..175723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            179627..188042
                     /locus_tag="NECAME_05222"
     mRNA            join(179627..179772,179845..179943,180071..180200,
                     180469..180561,181709..181812,183972..184073,
                     184138..184294,184352..184446,185719..185829,
                     185882..186001,186060..186194,186247..186329,
                     186399..186583,187809..188042)
                     /locus_tag="NECAME_05222"
                     /product="transporter, major facilitator family protein"
     CDS             join(179627..179772,179845..179943,180071..180200,
                     180469..180561,181709..181812,183972..184073,
                     184138..184294,184352..184446,185719..185829,
                     185882..186001,186060..186194,186247..186329,
                     186399..186583,187809..188042)
                     /locus_tag="NECAME_05222"
                     /inference="protein motif:HMMPfam:IPR005828"
                     /note="KEGG: cbr:CBG11705 Hypothetical protein CBG11705;
                     K08202 MFS transporter, OCT family, solute carrier family
                     22 (organic cation transporter), member 5 2.2e-181"
                     /codon_start=1
                     /product="transporter, major facilitator family protein"
                     /protein_id="ETN69509.1"
                     /db_xref="InterPro:IPR005828"
                     /translation="MLDLDFSHSLQTVITDSAANRTSLGTTKEVNKKMKFDDFLFANL
                     GEIGKYQRIQFFLVCLPTIIVSMHALSWTLASVPVPFRCALPSETHESPYYSSDERLT
                     LKECKGWDETPVSINESGSPRVHCYYNEKCELNGELCSSHVFDRSRVQYSAMDRWELV
                     CDRASNRAHVQLFYYIGQMVGSFVFGLLGDRIGRKKVCIIAIAMQVSCGLASVFAPTW
                     WLFAILRTGIGFSHPGIFVIAVVVGMELVGPKYRKMASVITGGFFALGQACSQFFSPN
                     FILLGVEGNFVTNYQHLQLLITLPGILFLSYWWLVPESPRWLVAQRSYEEADKILRRA
                     AKINGVTLPEKWWEQLDSEIDPSDKKSRKYKAWDLFKTPILRKRALVAFFLWPCVSMV
                     YYGMALKPNVLGGDMYLNFIFAALVEIPALIIVCLTIDRIGRRIVTSAGYFLAGACLL
                     VNFFLGDNVPLAVSIIQMMISRGAITGTYAAIYTYTPELFPTVIRNTAMGVCSMIARI
                     GAISATYLSLWIAETPNGKVYMIIPFACMAITAAALTLFVLPETMGTPLPETIAQVEG
                     DEEPLPQELEPLRTKESEETRQQLEPTSKNE"
     gene            189154..196060
                     /locus_tag="NECAME_05223"
     mRNA            join(189154..189280,189369..189490,193313..193467,
                     193523..193657,194193..194339,195677..195825,
                     195909..196060)
                     /locus_tag="NECAME_05223"
                     /product="hypothetical protein"
     CDS             join(189154..189280,189369..189490,193313..193467,
                     193523..193657,194193..194339,195677..195825,
                     195909..196060)
                     /locus_tag="NECAME_05223"
                     /inference="protein motif:HMMPfam:IPR005331"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69510.1"
                     /db_xref="InterPro:IPR005331"
                     /translation="MNRDIVLILTSFFVTSVFLNLVTRSSEDADKATPKVIAFSHKRK
                     FTANFDEIIDQIESETLQTAPFIAEHLVPPFYNYWSVYVTSPPFNFHHQLSTCLVEKT
                     MTTLRSAIMCFLSFHDEFVDRNRTISTESHSTRYCYKDNTFYNFPDAQASTKGNQTMF
                     SLVRHPIDRFLSGYVNKCIMEAPKDHRCYGCNENLNCFVERLYETLWEAYNSKMTEYD
                     FDLAHFAPQTWYCQYRTNLNNYVLVDYSTETEELVRQLDLVYGRAGVPQSYRVVISSE
                     IRRQRSNNSTSQTSDRASVERHPLIDEKTYRRLVQIYYYDFVVFGYSLPTFL"
     assembly_gap    189637..190051
                     /estimated_length=415
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    203264..204572
                     /estimated_length=1309
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    211820..211987
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            213833..215336
                     /locus_tag="NECAME_05224"
     mRNA            join(213833..213904,213971..214051,214112..214247,
                     214869..215054,215119..215336)
                     /locus_tag="NECAME_05224"
                     /product="hypothetical protein"
     CDS             join(213833..213904,213971..214051,214112..214247,
                     214869..215054,215119..215336)
                     /locus_tag="NECAME_05224"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69511.1"
                     /translation="MLHSLFPFLLLAHRGGSSEYQCFMVFVVLGRVVAMFTADKAAPY
                     FYAAISIIGCGVGCCIFVFDNGLLIGSAIFGFFLSSLLPSLIVYVRHTMVLPAHRLFV
                     LMSGSSFGYVIYPLFLTLANSSESRIFIAIDFFAVLLLVLIFVSILNLQAQTERTKPS
                     QDSLKIFIYRFFSGEAMLKRTRSIRPLISRLRPNSYRRFRSRHAPATSPRGTEVPKIE
                     VTLEEAPQIQYN"
     gene            complement(215520..224108)
                     /locus_tag="NECAME_05225"
     mRNA            complement(join(215520..215702,217549..217655,
                     217762..217921,217979..218119,218177..218305,
                     218953..219121,219183..219331,219425..219535,
                     220368..220437,220941..221017,224067..224108))
                     /locus_tag="NECAME_05225"
                     /product="stearoyl-CoA 9-desaturase"
     CDS             complement(join(215520..215702,217549..217655,
                     217762..217921,217979..218119,218177..218305,
                     218953..219121,219183..219331,219425..219535,
                     220368..220437,220941..221017,224067..224108))
                     /locus_tag="NECAME_05225"
                     /inference="protein motif:HMMPfam:IPR005804"
                     /codon_start=1
                     /product="stearoyl-CoA 9-desaturase"
                     /protein_id="ETN69512.1"
                     /db_xref="InterPro:IPR005804"
                     /translation="MMASPHKAAGLRMKVDGKWLHINEETIQNHPGGAVIRQYVNADA
                     THIFHAFHEGSRKAYKQLEALKKNAWDPSEDLEEALKLRLDKADVNISTYDISIEQEK
                     KIVESFERLRQRVHDWGLMEAQPWYYVAKAATTLGFMFFAFYLQYCGWYFTSALSLAI
                     SWQQFGWLTHEFCHQQPTKNRKLNDMFSIAFGNLAQGFSADWWKDKHNTHHATTNMID
                     HDGDINLAPLFAFVPADLSRYKLPVEKFILKFIPYQHLYFTLSLPLLRFSWTSQSLLW
                     VFAENSSEYRVYRKNALTEQSFLMAHWAWVFLQLYLLPSMGVRIMYFMVSQLLSAFLI
                     AYVVTFSHNSVDKYPANSRLLNNFACLQILTTRNMTPGPITDWLWGGLNYQIEHHLFP
                     TMPRCNLNTCMKLVKEFCKENNLPYLVNDYFEGYAMNLKQLQNIANISNTKSN"
     assembly_gap    224559..224658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(225910..228774)
                     /locus_tag="NECAME_05226"
     mRNA            complement(join(225910..225939,226340..226456,
                     226582..226661,226903..227032,227206..227276,
                     227334..227419,227516..227622,228565..228666,
                     228727..228774))
                     /locus_tag="NECAME_05226"
                     /product="putative multicatalytic endopeptidase"
     CDS             complement(join(225910..225939,226340..226456,
                     226582..226661,226903..227032,227206..227276,
                     227334..227419,227516..227622,228565..228666,
                     228727..228774))
                     /locus_tag="NECAME_05226"
                     /inference="protein motif:HMMPfam:IPR000426"
                     /inference="protein motif:HMMPfam:IPR001353"
                     /note="KEGG: cbr:CBG09365 Cbr-pas-6; C. briggsae CBR-PAS-6
                     protein; K02725 20S proteasome subunit alpha 6 1.9e-104"
                     /codon_start=1
                     /product="putative multicatalytic endopeptidase"
                     /protein_id="ETN69513.1"
                     /db_xref="InterPro:IPR000426"
                     /db_xref="InterPro:IPR001353"
                     /translation="MFRNQYDSDVTVWSPQGRLHQVDYAVEAMKQGSATVGIKSETHA
                     VLAALKRAQNELCSHQKKIYEIDTHAGVSIAGLLSDGRILARFLQTECSSWRWDYKQP
                     VPISSLSDSVQLKLQANTQYYGRRPFGVGLLIAGYDKDGTHIVQSDPSAEVVEMFASS
                     IGARSQSARTYLERHLDKFRLANADELIEHALLALRDTLPAEENLTSQNTSIAIVGKG
                     QPFKIMDDSEVAPHLSKISNTPRTIGQPEAPPAEQMQL"
     assembly_gap    234037..235066
                     /estimated_length=1030
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    235636..236655
                     /estimated_length=1020
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    248743..248842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    250898..250997
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            256492..264893
                     /locus_tag="NECAME_05227"
     mRNA            join(256492..256521,256621..256701,257432..257511,
                     257571..257661,257718..257837,262136..262229,
                     262319..262489,262750..262841,263399..263471,
                     263612..263753,263890..264017,264423..264510,
                     264569..264704,264759..264893)
                     /locus_tag="NECAME_05227"
                     /product="SH2 domain protein"
     CDS             join(256492..256521,256621..256701,257432..257511,
                     257571..257661,257718..257837,262136..262229,
                     262319..262489,262750..262841,263399..263471,
                     263612..263753,263890..264017,264423..264510,
                     264569..264704,264759..264893)
                     /locus_tag="NECAME_05227"
                     /inference="protein motif:HMMPfam:IPR000980"
                     /codon_start=1
                     /product="SH2 domain protein"
                     /protein_id="ETN69514.1"
                     /db_xref="InterPro:IPR000980"
                     /translation="MSILSEILSTGHVDKELLDALDDAQKQLLFCQMRAEQVRKWQAN
                     EERLEREGAPKKTANKRGIKWLTGADGEVWVWVMGDHPLDKSIEEILEEEARQEAHNR
                     ALKELTGQGDTEVKEPEEALKAQLGQMRVGVTDSIYDDSDTSFDPTHPLLFQPVRRDL
                     TPNGNGVASTNGASPARPVAVTATTVSSFTTPQPQKAELRGPPPPIPARPPNLTGKAA
                     VQVVGVKMRQPRDITWEQKAEDVQKRESEIFQSIQEKRERIRREAEAESERQRLEWEQ
                     QNQRAREAEEAMRKMAQKAREQHRQLIRTSTSILPALKDTKATSLREAIKNLPRPPKP
                     KSRAAIVDWFKRDEWPRGTGQDPKTRMPAPWFHGIISRDQAEILLQDKPTGSFLVRVS
                     ERIWGYTVSYVVGDGTFKHFLVERIPEGYQFLGTNQVVHDQLFDLISYHETAPITAKG
                     GEILKWSVGQMFRPPDYHDIIPDTAFPQANRLVGRF"
     assembly_gap    260917..261148
                     /estimated_length=232
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    264198..264297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            268085..270965
                     /locus_tag="NECAME_05228"
     mRNA            join(268085..268199,268281..268348,268450..268566,
                     268751..268898,268948..269041,269493..269646,
                     269803..269904,270081..270253,270608..270965)
                     /locus_tag="NECAME_05228"
                     /product="hypothetical protein"
     CDS             join(268085..268199,268281..268348,268450..268566,
                     268751..268898,268948..269041,269493..269646,
                     269803..269904,270081..270253,270608..270965)
                     /locus_tag="NECAME_05228"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69515.1"
                     /translation="MSKFVLERIHPLLQQLVLYQKASRDVKEILTVVKEFIEGEWAVV
                     DVQERAACEAAILIALRIGAESSISKGYWNRDRSRYAAVIHACLRRDNAKTSQYIQLN
                     SIKFRHVHSLLSALLSNIEGDSIVVFSVLQAIFDLTQYILCSDALRKEFLLPQCVPLY
                     GKLVSVSLEVLSNYSKDFRCRWVTLAILEVLVSSSKDSLSLCIVLPGLSSTLATTICE
                     SVNEHLDITTTSLKVISMAVRYSLADSAEDIANVGESKEIKPEILELLIRMLCSTLAV
                     HPDEHVRMTLLEVVDSIRTDCKKAFNGALDPFLLDLLLIVRGTTSFDSEVVLRLRAEN
                     VSAFSLHMHGKLRELAERIPLYVRKRTSNNDLMFHQVSLDILCFRRNCNCNCMKFFVL
                     VYVHFWCFLFMERFGSTKPVSFVLVQKKIQLSYSLAYLLFVRITQMRMKK"
     assembly_gap    273911..274971
                     /estimated_length=1061
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    275610..277376
                     /estimated_length=1767
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            279508..282655
                     /locus_tag="NECAME_05229"
     mRNA            join(279508..279682,281540..281643,282204..282302,
                     282440..282655)
                     /locus_tag="NECAME_05229"
                     /product="hypothetical protein"
     CDS             join(279508..279682,281540..281643,282204..282302,
                     282440..282655)
                     /locus_tag="NECAME_05229"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69516.1"
                     /translation="MVESSKSDRASLAILITLILAEMEPQDDSHILYLLTETCIDWIR
                     DARPEEHTRDDIIEHKVPVPSGETILSISLVSLVAVTFTRINEEKKQLKLLTAFLYEL
                     LGLYAITNWIVHDAADCALNQIASTMSLSVSEFLYERGTYLVHKIALASRSRREHYHA
                     PIVFSALLERVDDPRMYDHVRHIVEDLLLALDRFKQA"
     assembly_gap    279725..280478
                     /estimated_length=754
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            284236..285277
                     /locus_tag="NECAME_05230"
     mRNA            join(284236..284313,284873..285030,285088..285277)
                     /locus_tag="NECAME_05230"
                     /product="hypothetical protein"
     CDS             join(284236..284313,284873..285030,285088..285277)
                     /locus_tag="NECAME_05230"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69517.1"
                     /translation="MVHQNWEVLINRFRDKELEVRHEALKVVTQIVRLSKTFVYRKVR
                     YQMWPVLGKWMHDASIHTYSTTSVAYKYQLFVLQSVAEIFIGIEASSDDLNLVLEMLA
                     LYCNRTGTPQLKKEAESATKRLNVYLERRKRKKDLGEIW"
     gene            286754..288133
                     /locus_tag="NECAME_05231"
     mRNA            join(286754..286874,286933..287021,287520..287582,
                     287811..287913,288036..288133)
                     /locus_tag="NECAME_05231"
                     /product="riboflavin kinase / FAD synthetase"
     CDS             join(286754..286874,286933..287021,287520..287582,
                     287811..287913,288036..288133)
                     /locus_tag="NECAME_05231"
                     /inference="protein motif:HMMPfam:IPR015865"
                     /note="KEGG: nvi:100122227 NV13013; hypothetical protein
                     LOC100122227; K00861 riboflavin kinase 2.3e-35"
                     /codon_start=1
                     /product="riboflavin kinase / FAD synthetase"
                     /protein_id="ETN69518.1"
                     /db_xref="InterPro:IPR015865"
                     /translation="MLLICPVDYHMRLNRHLPYFLRGRVVAGFGRGGKQLGCPTANIE
                     EAVVETLPSEFPCGVFYGLARVDGEQVDKMVMSIGRNPHYHNEKKTLEVHLLRKFEND
                     FYGSMVDVLILGFIRPMAAFNNLDELRTAISSDIALAEKELKRMDTKQLTQQFFH"
     gene            complement(293992..294674)
                     /locus_tag="NECAME_05232"
     mRNA            complement(join(293992..294363,294657..294674))
                     /locus_tag="NECAME_05232"
                     /product="hypothetical protein"
     CDS             complement(join(293992..294363,294657..294674))
                     /locus_tag="NECAME_05232"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69519.1"
                     /translation="MSLFATRHRLILLDLLVDAPIEAFPLLLILACTMSHPSQTAGIR
                     AEQRGRRGERWTEQRRTNGVIAVFRAQRTGGPYSGCVWLHGTGCCSAAAAGQAASVAA
                     AASVGSSVCLDVGVSGMPALSGGAGVG"
     assembly_gap    297558..297657
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    298748..299386
                     /estimated_length=639
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    300999..301260
                     /estimated_length=262
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            308783..316000
                     /locus_tag="NECAME_05233"
     mRNA            join(308783..308800,309174..309302,310461..310615,
                     310759..310887,311083..311212,314900..315114,
                     315874..316000)
                     /locus_tag="NECAME_05233"
                     /product="g-protein alpha subunit"
     CDS             join(308783..308800,309174..309302,310461..310615,
                     310759..310887,311083..311212,314900..315114,
                     315874..316000)
                     /locus_tag="NECAME_05233"
                     /inference="protein motif:HMMPfam:IPR001019"
                     /note="KEGG: cel:R10H10.5 gpa-7; G Protein, Alpha subunit
                     family member (gpa-7); K04640 guanine nucleotide binding
                     protein (G protein), alpha, other 1.1e-156"
                     /codon_start=1
                     /product="g-protein alpha subunit"
                     /protein_id="ETN69520.1"
                     /db_xref="InterPro:IPR001019"
                     /translation="MRILHKDGFSQQDLEMIRPVVYSNCIHSMLSILRAMFHLQIEYA
                     DNERVRDSQLVFATVHANKEELTEELAAAMQRLWADEGVRECYRRSNEYQIDDSAKYF
                     LDNLPRLSSLNYIPSEQDLLRTRIKTTGITEVLFELKGLTFRVIDVGGQRSERKKWIH
                     CFDNVNAIIFISSLSEYDQTLREDNCTNRMQESLKLFDSICNSPWFADIHFILFLNKK
                     DLFAEKIQRSPLTICFPEYKGQQNQTECINYIQWKFEQLNRSSQREIYCHHTCATDTN
                     NVQFVLDACLDMIIAKNLKSMGLC"
     assembly_gap    313981..314080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    320801..320979
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(321186..322907)
                     /locus_tag="NECAME_05234"
     mRNA            complement(join(321186..321311,321399..321490,
                     322071..322160,322368..322497,322608..322664,
                     322746..322907))
                     /locus_tag="NECAME_05234"
                     /product="hypothetical protein"
     CDS             complement(join(321186..321311,321399..321490,
                     322071..322160,322368..322497,322608..322664,
                     322746..322907))
                     /locus_tag="NECAME_05234"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69521.1"
                     /translation="MSDVPSSLSGFEYTIDKTWDGLPIDHDPIHVKMKWLFARLPGKP
                     HKRVIKVNFQAPLFDDPEAPPDPPGILPGLWEYEVVEFFFANNRNQYLEVEVGPHGHW
                     LCLLFDGIRKPFNSGEELELEITNKFVGNVWHCEFEIPLAYLPGNITKFNSFAIHGTG
                     DERVYEALNPVTDGHYEKPDFHRLQFYGKINMRRLLPDGYGTKPFIDYKYGDVWKDHY
                     "
     assembly_gap    330144..330243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            333944..334334
                     /locus_tag="NECAME_05235"
     mRNA            join(333944..334007,334099..334334)
                     /locus_tag="NECAME_05235"
                     /product="hypothetical protein"
     CDS             join(333944..334007,334099..334334)
                     /locus_tag="NECAME_05235"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69522.1"
                     /translation="MVAPFLKYFFYRGVLPLSLLFAAFVRPCFMSVGYVIFALLSPVL
                     PSIHAAMPLPGGICMCCLWFLLGHCRATIYLDDLNHIDLLFFEGVQVFFSFLKRF"
     assembly_gap    337545..337644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            338550..341681
                     /locus_tag="NECAME_05236"
     mRNA            join(338550..338618,338708..338812,341245..341287,
                     341401..341681)
                     /locus_tag="NECAME_05236"
                     /product="hypothetical protein"
     CDS             join(338550..338618,338708..338812,341245..341287,
                     341401..341681)
                     /locus_tag="NECAME_05236"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69523.1"
                     /translation="MVALKRFSNFAIIVLAALVGCVQPSLLNCVYFLSFLSVASWWAL
                     YKPLRHGVYNRIKKVFLYRLSAKLCELGLVKMFYKPFIFVVDVVHDGIFPKADKRSEE
                     LPDGDSSLSELRGFFSPPCERKSSSKKAMVRSHIKLWYNSDIFQAKPFTDDYVVNFIM
                     IPFIG"
     assembly_gap    343138..343237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            345842..346190
                     /locus_tag="NECAME_05237"
     mRNA            join(345842..345904,345996..346190)
                     /locus_tag="NECAME_05237"
                     /product="hypothetical protein"
     CDS             join(345842..345904,345996..346190)
                     /locus_tag="NECAME_05237"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69524.1"
                     /translation="MVAIISFALYHAYTFALLSMMVWALLYHSIFGLILLVFSCTLWM
                     FKDSRGASFAVAPTVTIYVEVAYFEHCQFRTSERDNEEFGP"
     assembly_gap    355599..355698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    356368..356467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            357877..360057
                     /locus_tag="NECAME_05238"
     mRNA            join(357877..357909,358757..358861,359102..359193,
                     359898..360057)
                     /locus_tag="NECAME_05238"
                     /product="hypothetical protein"
     CDS             join(357877..357909,358757..358861,359102..359193,
                     359898..360057)
                     /locus_tag="NECAME_05238"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69525.1"
                     /translation="MRQLSDHGDSSTSFGFFCRIAYPFWLSLIIYTSVVIISLYVYQF
                     PDFPDIWTKWTGLDKKWNDDIGLINYSNAGKSGTLFVRLFTPISLFVVAMLQLKFFHE
                     PWMAMVRRLPNEQAQQGDASTSREYFL"
     assembly_gap    359752..359851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    365759..365858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            366433..366762
                     /locus_tag="NECAME_05239"
     mRNA            366433..366762
                     /locus_tag="NECAME_05239"
                     /product="hypothetical protein"
     CDS             366433..366762
                     /locus_tag="NECAME_05239"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69526.1"
                     /translation="MYAELVHIIDYYFHQLVATARRRNEAKALKSSNAKKLLTQNVMR
                     SSAISSADSGIDDMPFIDFGDDITTPIITARPFESYGPRKKPWGEHSGNFIVDDLISF
                     LNYSVWI"
     assembly_gap    369658..369757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <369816..387592
                     /locus_tag="NECAME_05240"
     mRNA            join(<369816..369938,370978..371112,371330..371446,
                     371524..371622,371865..371966,372653..372750,
                     372811..373132,373628..373734,373787..373893,
                     374025..374176,374247..374332,374389..374456,
                     374524..374686,375168..375278,375363..375421,
                     375876..375989,376732..376862,378310..378441,
                     378696..378750,378866..379023,379082..379176,
                     379853..379992,380061..380170,381398..381493,
                     381733..381837,381953..382262,382322..382472,
                     382531..382642,383588..383763,384741..384899,
                     384970..385117,385183..385312,385381..385621,
                     385680..385770,385912..385979,386174..386357,
                     386498..386680,386765..386928,386987..387161,
                     387509..387592)
                     /locus_tag="NECAME_05240"
                     /product="hypothetical protein"
     CDS             join(<369816..369938,370978..371112,371330..371446,
                     371524..371622,371865..371966,372653..372750,
                     372811..373132,373628..373734,373787..373893,
                     374025..374176,374247..374332,374389..374456,
                     374524..374686,375168..375278,375363..375421,
                     375876..375989,376732..376862,378310..378441,
                     378696..378750,378866..379023,379082..379176,
                     379853..379992,380061..380170,381398..381493,
                     381733..381837,381953..382262,382322..382472,
                     382531..382642,383588..383763,384741..384899,
                     384970..385117,385183..385312,385381..385621,
                     385680..385770,385912..385979,386174..386357,
                     386498..386680,386765..386928,386987..387161,
                     387509..387592)
                     /locus_tag="NECAME_05240"
                     /inference="protein motif:HMMPfam:IPR021999"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69527.1"
                     /db_xref="InterPro:IPR021999"
                     /translation="ATLSAKIVRWGNDAIDLTWRFTEVHIAKIVFIVVAVFAAKNICA
                     LYVPLVILVSLALLLPSAVSSVLSLIMCAYLCVVAVLKMIYQLSHFPDLSFVNNGEVC
                     NATKTFPEWVGIKKETDTWQLLGGMIVAIIVLALQSVIVYRQRHYRRMHGIPESTSDR
                     VFPSFNIDEFDHSLVHCMKFIVDFSFYKFGFETSVIMMAINAWVRMDMLAAVICIWLG
                     IFVLNKRSVCRKLWYIFVIYLAVLLPLQYVIYVGLPEDSCLGSFFFAILSSCLLLVMR
                     TDWFLAYPWDHIFGDSSSSTKNVNFDIWMGLSNYAVNWPAENLIADFLLLLVASCQLT
                     VFRNEGVDNDSIFVNEDYHLKPNNPRYDFIATQRSFVDFIKIAVFHYGHWITLIMTLI
                     AGIGGTSLFALGYIMLTFWILWQGNNLYVMNARTNSFKSTLAKWKTLLSYTVFCMFCK
                     VALQLVGCVFLEWFFDGSGVHRSMRCTIRQLFSIVCVNTVMQARKDAGAGPLFPHETD
                     LDRTCSVVQQEAQIGFDAVALAFLVFQIRIFHSWFFQHCMVEYRSEIILANRGAVLKN
                     QLIEKEMKEQNEQQSAEFDEIRKRTAAIRERYNKQMEKGEYIFDPQTYGQAYHTYDDE
                     DEVDDEEFFHFYYDAPRKSDEMAVEKTSPLAKRAGDYYMFDYDPSTDELVKPVESFVP
                     EVTPGATKFEKLDPGQLVYAATGHDLDLAKTLEQVKEAEKIKSNEGRMIEAMSREHPM
                     QPDTPMETAIEPSLKTEKPQESKISAFVKFALKMVVNAIEMLSAYLNRLSREHRYVAY
                     VLNKEKEKLKEGHSESLSDTSRRLTELRKRMNMTNLLLVQSEGDIQRMESAALTDWQQ
                     RSVLAKLVNALGSVIAANTDLICYLLAVMAHASGAGLITLPLPLMVFLWGTLSNPRPS
                     KFFWVAMIAYTEFVIVIKFICQFTIFEFNSISAQSKQAADPLTVEKVLGVQRQNNFAV
                     YDVPLLFALFFHRYMLRKLGLWKDANLTVTFQNDNAANDSEETYLEANTLFFLVFERL
                     LYLAFQYNYVQLILTVHCGLTSEKDACFFKDATQRSRGGKAVNGEAQKSADHQFVEST
                     SKGSSDGTKDEEKRTASGKDPEKTQPPANVAERTDAGQESAGNPIIRFINQLFKPKFR
                     YIKDLYPIMFGLDVVAFLIIAFGYSAFGEGGSGNVIGDIQSNRIPLTFVVMLFVMTLL
                     IVIDRGLYLRKWIPGKLAYQLLMIVFLHVWIFFVLPSLTRRAAMENTVAQAFYVIKCL
                     YLLVSAWQIRNGYPQLCIGNLLTHAYGLVNMVCFKIFMAVPFLFELRTAIDWTWTDTS
                     MPLFDFFNMENFFAVIYNLKCARNFEQSYPVPRGIPKGAIVKYMMGLPMILLIVFLVW
                     CPLLAFSLLNRIGAISIPDKVRLTIQLEGYPVITLVQLFNGFDYLSYCILRATLIDGS
                     CSMLQPIYEVESQGSELRPMRPEELKYLTDTMSRRYFPFPNGTESMKRSRDSVSFIKE
                     YSTNDVLVVNFRPESEVPWGISEESMSALIEQLNGNSSISFIVTAEISRPYDTQRKEV
                     TKHSAHWSQEIEANTALRRQWINMLQNPGSGQMVSMAEAFPSYLLVPSEGAVLVPTPI
                     VAAIELNQDNYQRPDNASDRDWFDTLQLSLVNSTMGNVWVVQAQHPSQFTSVYFNASK
                     ITYGTHRDRTYVQTIAFVDKAFPSFFAKYLQGGVIAMYVSLVIVVGRLIRALFTNSPT
                     DVMITEIPNPDFLLKICLDIYLVREAKDFFLEQDLFAKLIFLFRSPATLIQWTRHKTK
                     RD"
     gene            391931..392788
                     /locus_tag="NECAME_05241"
     mRNA            join(391931..392004,392081..392192,392618..392788)
                     /locus_tag="NECAME_05241"
                     /product="hypothetical protein"
     CDS             join(391931..392004,392081..392192,392618..392788)
                     /locus_tag="NECAME_05241"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69528.1"
                     /translation="MNFQAIICSFITAIGTVMICMTTRTVDDDGEFKGTAKTVILAIG
                     IALTAIGGLGYTFTVYEVRWRTHQPEDRGAYLAAKREAKKTVSKVKSDRYKGVYDMLD
                     SRERGRAVYRLVRARH"
     gene            393379..393966
                     /locus_tag="NECAME_05242"
     mRNA            393379..393966
                     /locus_tag="NECAME_05242"
                     /product="hypothetical protein"
     CDS             393379..393966
                     /locus_tag="NECAME_05242"
                     /inference="protein motif:HMMPfam:IPR000477"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69529.1"
                     /db_xref="InterPro:IPR000477"
                     /translation="MKVFECVLEARLRKIVSVLLNQCSFVKDCSTVNAIHAVRIPLEK
                     HREKNRGVHLVFCDLEKAFDRVPHELLWMSMRLHRVPEEYVRWTKLLYANSVSVVRCA
                     AGTSRPFPVQVGVHQDSSLSPLLFILCMDTITKLIQKQHPWTLLFADDVVLASESRDD
                     LQQQVQSWKDLQQHGLRLNVLQSEYMECEPRVEEG"
     gene            394359..395155
                     /locus_tag="NECAME_05243"
     mRNA            join(394359..394448,394923..395012,395072..395155)
                     /locus_tag="NECAME_05243"
                     /product="hypothetical protein"
     CDS             join(394359..394448,394923..395012,395072..395155)
                     /locus_tag="NECAME_05243"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69530.1"
                     /translation="MIWSRLAARRKFCCQNRSEARRFRREAGRTIKRGHEKLLKQEKR
                     KLEKQVHRRRKETAAKELARKETGRMASDEILMEAGEIWIEKI"
     gene            397667..400032
                     /locus_tag="NECAME_05244"
     mRNA            join(397667..397729,398683..398828,398888..399003,
                     399077..399255,399441..399557,399883..400032)
                     /locus_tag="NECAME_05244"
                     /product="hypothetical protein"
     CDS             join(397667..397729,398683..398828,398888..399003,
                     399077..399255,399441..399557,399883..400032)
                     /locus_tag="NECAME_05244"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ETN69531.1"
                     /translation="MLTTRQRPETLAWSPPGVDFKVAVVSDYAHYFDPNATTNQSRLE
                     LFTIDEIFRKPSANCASQHCVPCQYRFNELSWAPLKSETHPSGLIFGGTENGTVVFFD
                     AHKFVSSKSLSVVSSRRDHQGHVLSVDHSADNRWAISVGGAAQLLLWDLTNLATPFSP
                     GTSNFTDQVKKVRWNRSMDNILISLSSHRASLWDMRRPGGPILEFAELVICFRLEADV
                     IGRIFAGSRSIPPQSFSAIKWQQHQASKNGTYDILRVQ"
     assembly_gap    400649..401178
                     /estimated_length=530
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    403548..404227
                     /estimated_length=680
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
CONTIG      join(ANCG01013037.1:1..25206,gap(833),ANCG01013038.1:1..15266,
            gap(301),ANCG01013039.1:1..865,gap(1194),ANCG01013040.1:1..10462,
            gap(880),ANCG01013041.1:1..3725,gap(100),ANCG01013042.1:1..14329,
            gap(100),ANCG01013043.1:1..14638,gap(100),ANCG01013044.1:1..1021,
            gap(100),ANCG01013045.1:1..12270,gap(100),ANCG01013046.1:1..5096,
            gap(266),ANCG01013047.1:1..2306,gap(776),ANCG01013048.1:1..11650,
            gap(1017),ANCG01013049.1:1..12010,gap(100),ANCG01013050.1:1..16641,
            gap(100),ANCG01013051.1:1..13865,gap(100),ANCG01013052.1:1..10206,
            gap(100),ANCG01013053.1:1..13913,gap(415),ANCG01013054.1:1..13212,
            gap(1309),ANCG01013055.1:1..7247,gap(168),ANCG01013056.1:1..12571,
            gap(100),ANCG01013057.1:1..9378,gap(1030),ANCG01013058.1:1..569,
            gap(1020),ANCG01013059.1:1..12087,gap(100),ANCG01013060.1:1..2055,
            gap(100),ANCG01013061.1:1..9919,gap(232),ANCG01013062.1:1..3049,
            gap(100),ANCG01013063.1:1..9613,gap(1061),ANCG01013064.1:1..638,
            gap(1767),ANCG01013065.1:1..2348,gap(754),ANCG01013066.1:1..17079,
            gap(100),ANCG01013067.1:1..1090,gap(639),ANCG01013068.1:1..1612,
            gap(262),ANCG01013069.1:1..12720,gap(100),ANCG01013070.1:1..6720,
            gap(179),ANCG01013071.1:1..9164,gap(100),ANCG01013072.1:1..7301,
            gap(100),ANCG01013073.1:1..5493,gap(100),ANCG01013074.1:1..12361,
            gap(100),ANCG01013075.1:1..669,gap(100),ANCG01013076.1:1..3284,
            gap(100),ANCG01013077.1:1..5907,gap(100),ANCG01013078.1:1..3799,
            gap(100),ANCG01013079.1:1..30891,gap(530),ANCG01013080.1:1..2369,
            gap(680),ANCG01013081.1:1..920)
//