LOCUS KI658997 236821 bp DNA linear CON 23-MAR-2015 DEFINITION Necator americanus unplaced genomic scaffold N_americanus-1.0_Cont236, whole genome shotgun sequence. ACCESSION KI658997 ANCG01000000 VERSION KI658997.1 DBLINK BioProject: PRJNA72135 BioSample: SAMN02953824 KEYWORDS WGS; HIGH_QUALITY_DRAFT. SOURCE Necator americanus (New World hookworm) ORGANISM Necator americanus Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae; Bunostominae; Necator. REFERENCE 1 (bases 1 to 236821) AUTHORS Mitreva,M. TITLE Draft genome of the hookworm Necator americanus JOURNAL Unpublished REFERENCE 2 (bases 1 to 236821) AUTHORS Mitreva,M., Abubucker,S., Martin,J., Minx,P., Warren,C., Pepin,K.H., Palsikar,V.B., Zhang,X.W.E. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (17-APR-2013) The Genome Institute, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Necator americanus is a roundworm that causes most of the human hookworm infections. N. americanus is a blood-feeding nematode infecting people in the rural areas of the tropics and subtropics causing an estimated disease burden of 22 million disability adjusted life years. The life cycle of the parasite begins when parasite eggs from an infected host are passed in the feces. In favorable environmental condition they hatch, and through 2 larval stages develop to the infective stage (L3). L3s penetrate human skin, migrate through the circulatory system and lung to finally reside in duodenum. The development continues and the adult stages attach to the intestinal mucosa and feed on blood. The strain being sequenced was obtained from the laboratory of Dr. Peter Hotez by Dr. Bin Zhan, originally isolated from an infected patient in Hunan province, China, and has been maintained in hamster since 1976.The adult worms were collected from intestines of hamsters infected subcutaneously with N. americanus L3 for 8 weeks. Worm isolation and DNA extraction was performed by Bin Zhan with QIAamp DNA mini kit according to manufacturers instructions (Qiagen). Jian et. al., 2003 Exp Parasitol. This assembly consists of fragments, 3kb and 8kb insert whole genome shotgun libraries. The sequences were generating on the Roch/454 platform and assembled using Newbler. To improve scaffolding, an in-house tool CIGA (Cdna tool for Improving Genome Assembly), was used to map 454 cDNA reads using blat to the genomic assembly to link genomic contigs based on cDNA evidence. Only joins confirmed by additional independent data typing were accepted. The repeat library was generated using Repeatmodeler (A.F.A. Smit, R. Hubley & P. Green http://repeatmasker.org). The Ribosomal RNA genes were identified using RNAmmer (Lagesen et. al., 2007 Nucleic Acids Res.) and transfer RNA's were identified with tRNAscan-SE (Lowe and Eddy, Nucleic Acids Res. 1997). Non-coding RNAs, such as microRNAs, were identified by sequence homology search of the Rfam database (Griffiths-Jones et. al., 2003 Nucleic Acids Res.). Repeats and predicted RNA's were then masked using RepeatMasker (A. Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding genes were predicted using a combination of ab initio programs Snap (Korf, 2004 BCM Bioinformatics), Fgenesh (Salamov A., Solovyev V. 2000, Genome Res.) and Augustus (M. Stanke, et. al., 2008 Bioinformatics) and the annotation pipeline tool Maker (M. Yandell et. al., 2007 Genomic Research) which aligns mRNA, EST and protein information from same species or cross-species to aid in gene structure determination and modifications. A consensus gene set from the above prediction algorithms was generated, using a logical, hierarchical approach developed at the Genome institute. Gene product naming was determined by BER (http://ber.sourceforge.net). Our goal is to explore this WGS draft sequence of N. americanus to better define proteins involved in nematode parasitism that impact health and disease and are relevant to both host-parasite relationships and basic biological processes. For information regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. For specific questions regarding the N. americanus genome project contact Makedonka Mitreva (mmitreva@genome.wustl.edu) at Washington University School of Medicine. The National Human Genome Research Institute (NHGRI) of the National Institutes of Health (NIH) provided funds for this project. ##Genome-Assembly-Data-START## Finishing Goal :: High-Quality Draft Current Finishing Status :: High-Quality Draft Assembly Method :: Newbler v. MapAsmResearch-04/19/2010-patch- 08/17/2010 Assembly Name :: N_ americanus_v1 Genome Coverage :: 26.15x Sequencing Technology :: 454 ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..236821 /organism="Necator americanus" /mol_type="genomic DNA" /submitter_seqid="N_americanus-1.0_Cont236" /host="Homo sapiens" /db_xref="taxon:51031" /chromosome="Unknown" /lab_host="hamster" /geo_loc_name="China: Hunan Province" gene complement(1867..6483) /locus_tag="NECAME_09005" mRNA complement(join(1867..1947,3085..3217,3335..3423, 3687..3777,3867..3985,4066..4182,5237..5338,5400..5617, 6378..6483)) /locus_tag="NECAME_09005" /product="hypothetical protein" CDS complement(join(1867..1947,3085..3217,3335..3423, 3687..3777,3867..3985,4066..4182,5237..5338,5400..5617, 6378..6483)) /locus_tag="NECAME_09005" /inference="protein motif:HMMPfam:IPR013861" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80683.1" /db_xref="InterPro:IPR013861" /translation="MKTVLGDLQIVVERHSDVLRNNALPFRVLIILRVLGFALSLVAA AYGFLSLLPFQLLSIEVWRLLTCNFVGRNILLFAWTIWSLHFGTNLIRQNNSNESVLK IYAITQIFTTVLVCGAAYGVYALTSKTTLLYEDHIVDMVPFLPDSILLETPLGRIKYT HVPLLAVLSVSIFSIAGLMNPVVVLQCVLAVQISWTYLRFFNPHEVDSFYGDSSDHFV WASLFPRFFQPFCTILGRICFRSLVKLGVCKRSVRHVDLNSLQSVGISLRSLDSSARD AERRRQKALRDLNERLHKTKKLETIAWDEDAEDEKDATVETEKKQIVKQANNEEQPEE PVTMTSTQPIESVESHP" assembly_gap 2013..2672 /estimated_length=660 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 3579..3678 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 5843..5942 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 7125..7502 /estimated_length=378 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(8089..13561) /locus_tag="NECAME_09006" mRNA complement(join(8089..8266,8519..8616,9018..9092, 9229..9332,9394..9597,10849..10898,11032..11144, 11322..11429,11625..11787,11846..11921,13205..13343, 13403..13561)) /locus_tag="NECAME_09006" /product="zinc knuckle" CDS complement(join(8089..8266,8519..8616,9018..9092, 9229..9332,9394..9597,10849..10898,11032..11144, 11322..11429,11625..11787,11846..11921,13205..13343, 13403..13561)) /locus_tag="NECAME_09006" /inference="protein motif:HMMPfam:IPR018957" /codon_start=1 /product="zinc knuckle" /protein_id="ETN80684.1" /db_xref="InterPro:IPR018957" /translation="MIATRQLVSVYQQPTSTGMRLKIPLTMDDVIGGIDAEITDVEEA LMHQIGAREVPFAGMDKSGRGVCLKHVQGTCTMNLVCPLRHIVGDKAVVCKHWLRGPY CKHRHRRRMICPNYIAGFCPDGMEECKFTHPSFALPAPENPVANNKRLFNHQIICHNC HERGHKATHCPHLPTQQAKQSDSNPMTNRATTTVQPVDLSLFPDKKSLSEVTCYKCGE KGHYANRCHKGALAFLSNTAHLAQEQREKDEREGRYLPGAYMGQPSLNPRTGTVFQGY LIDATTVIDCLHTFCKSCLLTYFEEEDNCCPTCEQLIHQSHPSHYVAFDRTMQDIVYK LVPGLLEAERKRRLEFLKNKKREAGETVEEDEEDTEKKDESPQGTNRCFEGEPGISHH RNDEHVIVSLIPGAPELPTPVRPIVRLSGMATVNTLKRYLAQSMWSNMSRYSELDLFC NDELMGRDFSMRFIHLTRCRNKLKDEPLKLVYRYHIDF" assembly_gap 12262..12979 /estimated_length=718 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 15284..18096 /locus_tag="NECAME_09007" mRNA join(15284..15355,15810..15903,16030..16172,16423..16507, 16599..16675,16812..16991,17116..17304,17360..17446, 17591..17698,17794..17943,18010..18096) /locus_tag="NECAME_09007" /product="hypothetical protein" CDS join(15284..15355,15810..15903,16030..16172,16423..16507, 16599..16675,16812..16991,17116..17304,17360..17446, 17591..17698,17794..17943,18010..18096) /locus_tag="NECAME_09007" /inference="protein motif:HMMPfam:IPR018800" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80685.1" /db_xref="InterPro:IPR018800" /translation="MMGLVDYGSDSGSEADEAPEDVGTSAVVSSSKSAPLPSTSNGQD DSYFAADDVFEGSSALNLPSVIESTSTTLVTEGELEDIVKPKEWELKMAEKERRRKEK KAKKKEKKRKDKETPSTESLPNVREKTPRGKAKIAAFGALNAIVDGVDNESDEEQTAK PSNLPSKPKGCGLLAMLPTPKGGARKSGLGGAGVKGTGILLPPSLRNKSKATPTVLKV VNETTATKRPAAGSDDSDDDDLPTDFFGLSSAPEPKVPKVGDIPALVNGVADVMGPSR LQIQEDIDDGYGYPEVEPSSSDMAPPEGVITDEEAHRLIMKYEINPMGLDHQRSYNSV AAEIVDIRVDDALGPDVRANILKNLHTTARAKDATAPLPKSKNPADATSRRKHQITYL ANLAVAREEALQQQWAESKQMRRIGRQKYGF" gene 21014..23443 /locus_tag="NECAME_09008" mRNA join(21014..21093,21184..21265,21899..21992,22056..22141, 22193..22368,22473..22600,23244..23443) /locus_tag="NECAME_09008" /product="membrane complex biogenesis protein, BtpA family" CDS join(21014..21093,21184..21265,21899..21992,22056..22141, 22193..22368,22473..22600,23244..23443) /locus_tag="NECAME_09008" /inference="protein motif:HMMPfam:IPR005137" /inference="protein motif:HMMTigr:IPR005137" /codon_start=1 /product="membrane complex biogenesis protein, BtpA family" /protein_id="ETN80686.1" /db_xref="InterPro:IPR005137" /translation="MKKAVRVVKECARPFIFGMVHVPALPGTPLNKMSMPEILQIVRK ETEIYANAAVDGIIVENMHDIPYVKPPIGPEITSAMSLACRTVTETLGKKREQMLLGV QILAGANKEALASLYIISSSGFDLIRAECFVFSHVADEGWMDGCAGELLRYSRTIGAD SVAIITDVKKKHSSHAVTADLSIGDVAEAAQFFMADGVIVTGRCTGHEADVSDIQEIR SACSLPIFVGSGVTVSNVHQFGNADALIVGSHFKKDGKWHNELEPQRVFQFMERVRTH KWCQT" assembly_gap 24343..24442 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 26184..27225 /estimated_length=1042 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 29121..29220 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 30143..31035 /locus_tag="NECAME_09009" mRNA join(30143..30145,30355..30423,30488..30601,30661..31035) /locus_tag="NECAME_09009" /product="hypothetical protein" CDS join(30143..30145,30355..30423,30488..30601,30661..31035) /locus_tag="NECAME_09009" /inference="protein motif:HMMPfam:IPR003677" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80687.1" /db_xref="InterPro:IPR003677" /translation="MDLNLTRKQYEDGMNKWAKENGVEEQYANYTKQQEEEKNELEEG LKQISRYFRKNRELENNTSITYAQAEENRQKFLAKLTPKQKVAKQFLECLFTPTSAKD CFRPMGPFPVPGPVVPGPTGPETEVPGPTGPGPVIPVPTGPGPYGPGPMNPYGPGIMN PYGPGTMYPYGPVPTNPYAPFLPRCT" assembly_gap 37738..37929 /estimated_length=192 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 41282..43120 /locus_tag="NECAME_09010" mRNA join(41282..41374,41728..41856,42983..43120) /locus_tag="NECAME_09010" /product="hypothetical protein" CDS join(41282..41374,41728..41856,42983..43120) /locus_tag="NECAME_09010" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80688.1" /translation="MRTIFIVLFFVAVVSPWVIYRRNHKKRLVCKEEGEMNGTLAEDS AEEQPMVATIRLKRDVEVDPLHKQPIIYLGTQSNSSKFIDDGDFDIVSDHLTPEELKE VKQRIMDTCNALNTNPN" gene 45451..47617 /locus_tag="NECAME_09011" mRNA join(45451..45545,45624..45718,46172..46249,46340..46492, 46551..46786,46963..47091,47142..47293,47357..47435, 47555..47617) /locus_tag="NECAME_09011" /product="hypothetical protein" CDS join(45451..45545,45624..45718,46172..46249,46340..46492, 46551..46786,46963..47091,47142..47293,47357..47435, 47555..47617) /locus_tag="NECAME_09011" /inference="protein motif:HMMPfam:IPR007846" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80689.1" /db_xref="InterPro:IPR007846" /translation="MYSSPLLNRSAGISSSLSVAPSDDALSGMNRRFVGDNQDDSPAK APAFLFGQKRRSVAPGTPSYLNNPYSGQSAPSNDIFASPIPSQLRGETASNSGKSVHW SPALVQERSHPHDTSRPNLKSLSSQASGPFTPSGTLSPAPPLRSMRDDIEPVRKVPRR SMSITASDSPFSVAGASKPATDSLQTAADTWVTVYGFPPEQAANVLKYFSRHGEIVSH QVPSRGNWMHIRYSCPVHARQAISRNASLIGNSLRVGVIPCTEKEIVGADVSQVISPI LNRSSVMEQSIIDEEPQEIPAITPSKENHDAVDESMNRSRLSMSSRAGMRSLTVSYDS RQDPKNPQPVKHDSIINKLWTAVGL" gene complement(48014..57018) /locus_tag="NECAME_09012" mRNA complement(join(48014..48184,49513..49588,50145..50273, 50586..50646,50749..50863,50921..51033,51076..51132, 52412..52519,52578..52678,52842..52916,52981..53063, 53789..53884,53943..54077,54164..54252,56063..56186, 56615..56697,56812..56933,56987..57018)) /locus_tag="NECAME_09012" /product="degenerin" CDS complement(join(48014..48184,49513..49588,50145..50273, 50586..50646,50749..50863,50921..51033,51076..51132, 52412..52519,52578..52678,52842..52916,52981..53063, 53789..53884,53943..54077,54164..54252,56063..56186, 56615..56697,56812..56933,56987..57018)) /locus_tag="NECAME_09012" /inference="protein motif:HMMPfam:IPR001873" /codon_start=1 /product="degenerin" /protein_id="ETN80690.1" /db_xref="InterPro:IPR001873" /translation="MLGQAPNLIYRIVWIILLSGCAFMFIYQAIAVVDKYSRMDKITD IQLKFDTAPFPAITLCNLNPYRDSKIRDEASINKILSVFKKMMNKAAGASELGELENV VDDLKVTLKYDRRKRNVEILLQHPTPTDQLCICAFDRLTHDAWPCHPREQWLNTTCQH CDDHRFCQKRSKTGVRKNEPCLCEKGESFCMLYDRIAKILNLWEFFGSSHDYNAEVSE DETEALGFGNMTDEVAIVTKAKENIIFAMSALSEEQRRAMSQAKHNLIHKCSFNGKPC DIDKDFAIISDPTFGNCFTFNHNRSDFKSSLRAGPMYGLRVMLFVNASDYLPTSEAVG VRLTIHDKDEFPFPVRHIRLQCTYRLYFLVWNENDGYRLQKKMSRLPAPYGDCVAQGA TSNYVYKGYTYSTEGCYRTCFQQLIIDRCGCGDPRFPSIGEHQHCQVFNKQHRACLEQ STHELGDIHGSFKCRCQQPCNQTIYTMSYSEAIWPSQSLNITLGTCEEEPEICNEQYQ ENAAMLEVFYEALNFETLTESEAYGVVKMLADFGGQLGLWSGVSFMTCCEFVCLGCEI LYMIIMHHWKKYKLKKHEESNEF" assembly_gap 59183..60225 /estimated_length=1043 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 61380..63603 /estimated_length=2224 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 63834..66363 /locus_tag="NECAME_09013" mRNA join(63834..63918,64007..64131,64336..64446,64506..64577, 66277..66363) /locus_tag="NECAME_09013" /product="ADP-ribosylation factor family protein" CDS join(63834..63918,64007..64131,64336..64446,64506..64577, 66277..66363) /locus_tag="NECAME_09013" /inference="protein motif:HMMPfam:IPR006689" /inference="protein motif:HMMTigr:IPR005225" /note="KEGG: cbr:CBG17727 Cbr-arf-3; C. briggsae CBR-ARF-3 protein; K07939 ADP-ribosylation factor 4 3.8e-81" /codon_start=1 /product="ADP-ribosylation factor family protein" /protein_id="ETN80691.1" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR006689" /translation="MVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTV WDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIEESREELHKMLNEDELRDATLLV FANKQDLPNAMSAAELTDKLGLHNLRSRQWYIQATCATQGHGLYEGLDWLSNQLSKS" assembly_gap 65494..65930 /estimated_length=437 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 69137..70754 /locus_tag="NECAME_09014" mRNA join(69137..69282,69732..69925,70066..70146,70203..70281, 70628..70754) /locus_tag="NECAME_09014" /product="hypothetical protein" CDS join(69137..69282,69732..69925,70066..70146,70203..70281, 70628..70754) /locus_tag="NECAME_09014" /note="KEGG: lch:Lcho_0958 OmpA/MotB domain-containing protein; K03286 OmpA-OmpF porin, OOP family 1.8e-10" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80692.1" /translation="MWTILSVLLISAVVAEYVDEPVGPDGGQTKPLPKSYPEAPQQYV TNGASACNQPQSDAARRPAPRPAPRPALRPAPRPAPRPAPRPAPRYPPRPAPRPAPRY PPRPAPGPAPASNPSPQVAPTYPAGTAPGSGQGQSGGTGNPNPHGGRLSLSLSDAVRP RPVPRPQPRPMPVIRTPSARVALPSQNSGQIKPQAAPMSDAPANPYRQ" assembly_gap 69421..69520 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 70756..70855 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 74016..74115 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 77848..78950 /estimated_length=1103 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 81734..81833 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 87379..87662 /estimated_length=284 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 90832..92251 /estimated_length=1420 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 93126..95435 /locus_tag="NECAME_09015" mRNA join(93126..93265,94587..94648,94759..94839,94912..95041, 95249..95435) /locus_tag="NECAME_09015" /product="hypothetical protein" CDS join(93126..93265,94587..94648,94759..94839,94912..95041, 95249..95435) /locus_tag="NECAME_09015" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80693.1" /translation="MRAVLSLLLIGVVIAEYVDEPIGPGGGQPSPKSYPEGPQQYVSN GASANYQPQSDAVVKPAPVPTPKEKPVKGTPTVIGKSPTPSKGVITPTKTGGTISHGG KTGGKPPVAPPGTKPAPKPEPKPLPVPEPKPAPKPAPHSVIRTPQAVRGSGAPPTPPK SGPPTPPKNGPKPDAKIPPPPVAPAKPPLSDAPANPYRH" gene complement(96234..104810) /locus_tag="NECAME_09016" mRNA complement(join(96234..96359,104280..104387, 104793..104810)) /locus_tag="NECAME_09016" /product="hypothetical protein" CDS complement(join(96234..96359,104280..104387, 104793..104810)) /locus_tag="NECAME_09016" /inference="protein motif:HMMPfam:IPR007965" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80694.1" /db_xref="InterPro:IPR007965" /translation="MLTWLKILCLLDFYVHFSEQRKGHGKAIIDYMLQNEHAEAYQVI HSAYECHSIVQISALQSGSNLQSLYSETVTPDPGVTVSK" assembly_gap 96499..96866 /estimated_length=368 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 98656..98765 /estimated_length=110 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 101736..104222 /estimated_length=2487 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 105478..105577 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 105661..113786 /locus_tag="NECAME_09017" mRNA join(105661..105840,105905..105976,106031..106129, 106189..106353,106433..106531,106589..106744, 106864..106962,107410..107517,109710..109823, 109855..109974,110032..110145,110222..110320, 110488..110601,110670..110768,111172..111285, 111337..111435,111497..111610,111687..111785, 111941..112054,112155..112253,113739..113786) /locus_tag="NECAME_09017" /product="hypothetical protein" CDS join(105661..105840,105905..105976,106031..106129, 106189..106353,106433..106531,106589..106744, 106864..106962,107410..107517,109710..109823, 109855..109974,110032..110145,110222..110320, 110488..110601,110670..110768,111172..111285, 111337..111435,111497..111610,111687..111785, 111941..112054,112155..112253,113739..113786) /locus_tag="NECAME_09017" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80695.1" /translation="MKVCLVLLGFTACAFAQYDTQPSGYGNNNPAAVQYVAKKSPRSK PVDYTKTSEDTPVRRPTPYQPPQPTYNVIGNKFSQQNLGQTNTQGQSSAQIGGAQTNV GLNQNAVNVGQTNNQNVGGGGSGSYRPSYDAPSYGAGAGGAIIIPVLPSYAGAGGSNV IGNKIDSQTLGQVNTQGQGNYQQGGAQTNVGANANTVNVGQQNYQGGGGGGGSGYRPS YDAPSYGAGAGAGYGGGPIYIPDFNNFIKNNVYSQNLGQTNTQGQANYQQGGNQANVG VNTNEVNVGQTNNQNVGGGKASYDAPRYGGYQPTYGGYQPPYGGYQPSTNGGSAGSYR PPHDTPTYAAILVPNYNVIGNKIDSQNLGQSLKQSFQINTQGQQNVQKGGDQTNVGLN ANLVKIGQTNTQEGAAGSYRPSYDVPTYAAVLIPNYNIVGNKIDAQTLGQINTQGQQN VQKGGDQTNVGLNANQVNVGQANAQGGAGGRYQPSYDVPTYAAILIPNYNVLGNRYDV QNLGQVSNQGQQNVQKGGDQTNVGANANQVNVGQANTQGGAVSGYRPSYDVPTYAAVL IPNYNVIGNKFDSQTLGQINTQGQQNIQKGGDQTNVGANANQVNVGQTNTQGGASGSY RPSYDVPTYAAVLIPNYNVVGNNIDAQTLGQINTQGQQNVQKGGDQTNVGLNANQVNV GQANTQGSAGGRYQPSYDVPTYAAILIPNYNVVGNRYDVQNLGQVSNQGQQNVQKGGD QTNVAANANQVNVGQANTQAKRLWKQLPQAILVP" assembly_gap 107558..109107 /estimated_length=1550 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 110900..110999 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 112388..113433 /estimated_length=1046 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 119319..120145 /estimated_length=827 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 120996..121095 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 122595..122694 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 123318..123848 /estimated_length=531 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 124517..124805 /estimated_length=289 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 125498..125597 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 125892..128376 /locus_tag="NECAME_09018" mRNA join(125892..126140,126196..126471,126529..126753, 126809..126973,127028..127126,127184..127330, 127414..127512,127568..127684,127851..127949, 128096..128221,128290..128376) /locus_tag="NECAME_09018" /product="hypothetical protein" CDS join(125892..126140,126196..126471,126529..126753, 126809..126973,127028..127126,127184..127330, 127414..127512,127568..127684,127851..127949, 128096..128221,128290..128376) /locus_tag="NECAME_09018" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80696.1" /translation="MYFFALLLLGVGCAFANYDTQPSGYGNNNAYEVQHMAKKSPYNK PVDYTKSNDFPSGFNFFGNKFSQQNLGQHNQQGQTNVQKGGDQKNVGANQNEVNVGQQ NKQKVRGYDAPYRPTYPGGYNPNPATNILGNNFDLQTLGQVNTQGQTSVQQGGDQTNV GSNANAVNVGQTNTQGGVSGGYKPSYDAPRPYGGYQGGYNPATNIFGNNFDLQTLRQV NTQGQQNAQIGGAQTNAGVNQNAVNVGQTNTQGGAGAGAGYRPSYDAPSYGAVLIPIN PSTGGYAGGYAGAGGNVVGNKFDVQNLGQVSSQGQANLQKGGDQTNVGLNQNAVNVGQ QNNQGGAGAGYKPSYDAPSYGAGAGAGYNGGPIYVPDFNNFIKNNVNSQTLGQANTQG QVNVQKGGDQTNVGSNANTVNVGQQNNQGGNGGSYGKPSYDVPRYAAILIPNYNVIGN KIDSQTLGQANTQGQTNVQKGGDQTNVGSNANAVNVGQTNAQGTVGTGPSYDEPRRGG FTAIGNRVKQQNLGQRNLQGQGSYQQGGAQGNFGFNSNVVNVSQTNNQSVNGVK" gene 131971..134478 /locus_tag="NECAME_09019" mRNA join(131971..132213,132267..132554,132622..132849, 132903..133067,133122..133220,133278..133424, 133507..133605,133686..133802,133962..134060, 134151..134270,134392..134478) /locus_tag="NECAME_09019" /product="hypothetical protein" CDS join(131971..132213,132267..132554,132622..132849, 132903..133067,133122..133220,133278..133424, 133507..133605,133686..133802,133962..134060, 134151..134270,134392..134478) /locus_tag="NECAME_09019" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80697.1" /translation="MHIALLLLGVGCAFANYDTQPSGYGDNNAYDVQHMAKKSPHNKP VDYTKSNDFPGFNFIGNKIAEQTLGQVNTQGQSNVQKGGDQTNVGLNQNEVNVGQVNN QNTGSGKRGYDAPYRPTYPGGYNPNPATNILGNNFDLQTLGQVNTQGQTSVQQGGDQT NVGSNANTVNVGQTNTQGGVGTGGYKPSYDAPRPYGGYQGGYNPATNVLGNNFDLQTL GQVNTQGQTSVQQGGDQTNVGSNTNAVNVGQTNTQGGAGAGAGYRPSYDAPSYGAVLI PINPSTGGYAGGYAGAGGNVVGNKFDVQNLGQVSSQGQANLQKGGDQTNVGVNQNAVN VGQQNNQGGAGAGYRPSYDAPSYGAGAGAGYNGGPIYVPDFSNFIKNNVNSQTLGQAN TQGQVNVQKGGDQTNVGSNANTVNVGQQNNQGGNGGSYGKPSYDVPRYAAILIPNYNV VGNKIDAQTLGQVNTQGQSNVQQGGDQTNVGSNANAVNVGQANTQGTAGTKPSHDAPR GFFYAGNRYSGQNLGQGSTQGQGNYQQGGAQGNFGVNSNVINVSQTNTQTVDGFK" gene 143963..146417 /locus_tag="NECAME_09020" mRNA join(143963..144205,144260..144541,144602..144838, 144895..145131,145195..145359,145414..145512, 145570..145716,145814..145882,145938..146063, 146121..146417) /locus_tag="NECAME_09020" /product="hypothetical protein" CDS join(143963..144205,144260..144541,144602..144838, 144895..145131,145195..145359,145414..145512, 145570..145716,145814..145882,145938..146063, 146121..146417) /locus_tag="NECAME_09020" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80698.1" /translation="MHIALLLLGVGCAFANYDTQPSGYGNNNAYDVQHVAKKSPYNKP VDYTRSNDFPGFNFFGNKVSQQNLGQTNTQGQSNFQKGGDQTNAGLNQNQVNVGQQNK QKVGSRGYDAPYRPTYPGGYNPNPATNILGNNFDLQTLGQVNTQGQTSVQQGGDQTNV GSNANAVNVGQMNTQGGVSGGYNGGYKPSYDAPRPYGGYQGGYNPTTNVLGNKFDLQT LGQVNTQGQTSVQQGGDQTNVGSNANAVNVGQTNTQGGVSGGYNGGYKPSYDAPRPYG GYQSGGYNPFNVLGNNFDLQTLGQANTQGQQNAQIGGAQTNAGYNQNAVNVGQTNNQG GAGAGAGYKPSYDAPSYGAVLIPINPSTGGYAGGYAGAGGNVVGNRYDIQNLGQVSSQ GQANLQKGGDQTNVGLNQNAVNVGQQNNQGGAGAGYKPSYDAPSYGAGAGAGYNGGPI YVPDFGNFIKNNVYSQNLGQKGGDQANVGVNANTVNVGQTNNQGGAGGKSYDAPQQGF NPFNFINNKVFQQNLGQTNTQGQSNAQIGGAQTNFGQNLNTVNVGQTNNQFSDVPTYG KPAPPAYKPAPPAYKPSPPAYKPSPPAYKPSPPAYKPSPPAYKPSPPAYKPSPPAYKP EPESSGYRH" gene complement(148246..151916) /locus_tag="NECAME_09021" mRNA complement(join(148246..148387,151837..151916)) /locus_tag="NECAME_09021" /product="hypothetical protein" CDS complement(join(148246..148387,151837..151916)) /locus_tag="NECAME_09021" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80699.1" /translation="MEVAADLRPVLGPALVRLDPMRIKQLQSPTVYKAIDDLAKLSAQ CMQLRAPLTCCEKLVMSDHTLYLSWEYDQ" assembly_gap 150365..150557 /estimated_length=193 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 157717..157816 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 158709..160501 /estimated_length=1793 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 161257..162141 /estimated_length=885 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 163263..165397 /estimated_length=2135 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 165939..168289 /estimated_length=2351 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 168953..169052 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 170047..170532 /estimated_length=486 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 172550..173823 /estimated_length=1274 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 175212..176255 /estimated_length=1044 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 183898..183997 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene <184100..191040 /locus_tag="NECAME_09022" mRNA join(<184100..184219,184414..184501,184585..184715, 189240..189378,190835..191040) /locus_tag="NECAME_09022" /product="hypothetical protein" CDS join(<184100..184219,184414..184501,184585..184715, 189240..189378,190835..191040) /locus_tag="NECAME_09022" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80700.1" /translation="DDARTRWFLNGAVISGTEAGVELSQNNRLLSLTNQYLLKQGRSE HKLDCKVVAESGRAFDQRSLKIDVIDAPALRPLSGEVLQPLGSELNLHCSQKKRNGLS STIKWFFNGREVAVKRGRLVVKELSQRDYGVYQCEATNEAGSSMNTVWVKEGASQNRA LPQSDQPVSSEESSQENEFGAPVITKPPKDVSFSVGSTTLKLPCVVTGTPVTTTTWRF NGESFCCSI" assembly_gap 184829..184928 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 187462..187561 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 191890..193105 /estimated_length=1216 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene <194565..195712 /locus_tag="NECAME_09023" mRNA join(<194565..194678,194735..194843,194919..195040, 195369..195440,195575..195712) /locus_tag="NECAME_09023" /product="fibronectin type III domain protein" CDS join(<194565..194678,194735..194843,194919..195040, 195369..195440,195575..195712) /locus_tag="NECAME_09023" /inference="protein motif:HMMPfam:IPR003961" /codon_start=1 /product="fibronectin type III domain protein" /protein_id="ETN80701.1" /db_xref="InterPro:IPR003961" /translation="PTQSPKNVIANVLNSTAVQIRFTAPDQQRIPGVNLGYKVEFWKG IPQKGVLYRQVMVDPTSKDLAVIVEGLEKFGHYNVTVLCYTSPGDGPRSHPVQVVTDE DIPGAVSGLSIAEVMFNGAVVVWDIPEHPNGVITRFVLRYWNDAFPNDITVQEFDGTQ RNLTIEGLSPSTRYTVGFSVFSVI" assembly_gap 197486..197585 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 201609..205346 /locus_tag="NECAME_09024" mRNA join(201609..201695,201870..201986,202279..202401, 202475..202558,202661..202820,202879..203003, 203182..203298,203475..203586,203664..203771, 203829..203946,204001..204198,204613..204735, 204879..205067,205178..205346) /locus_tag="NECAME_09024" /product="fibronectin type III domain protein" CDS join(201609..201695,201870..201986,202279..202401, 202475..202558,202661..202820,202879..203003, 203182..203298,203475..203586,203664..203771, 203829..203946,204001..204198,204613..204735, 204879..205067,205178..205346) /locus_tag="NECAME_09024" /inference="protein motif:HMMPfam:IPR003961" /codon_start=1 /product="fibronectin type III domain protein" /protein_id="ETN80702.1" /db_xref="InterPro:IPR003961" /translation="MAEPLSSTSLSVRWDPWNEDGDAAISGFRVRFVPVTSVLSESSH EEELMVVENNSCVITDLRKYTEYQISVTPYNRAGEGGVSQVRVRTLEDVPGTVGALRF SDILLDSVRVTWKPPTEPNGIITGYIVNYRTFKMKEEFKKEVQSRTQQTFYPATNLEE GVTYFFAVWAETSAGRGTEVTGNVTLGPNPDGPPAPSRPALTPGPSSVTLEWKDEKRD TVIGHLIQAKLVSKDTNAALEKKRHKRSFGRPTHIHGIWVTLRIVEGAGQQHEVSYRE LEPSSFYVFRVFARNELGIGKPSAETEQLFVPAVIPEDPFYTTWWFIAMVAMSTFVVI VLLVAFLCITGSVAKYKREKRNSVDSIHLANGNFMAFQMEASHRDIGRSRNELSTRPG TKQSWLSDREPPAYGSILGDQERLRGRESIDGGGSVVNMYGLETDVIPPVPNQEAMQR LTALVGRDVRGSAYVSQRDSIDILNRSKYGSRADYGSPMNRIQYSRADYGTRVGYSHT ENNAQSEEFADDSFDDEDDGSGSSKEEIENGGENIASHYGSTDQYRDTWRKVKANEAI RGAPPPPPTTSSRAVSTESGSESSSAWQPQPAPNLSSGFSSFV" gene 209125..211255 /locus_tag="NECAME_09025" mRNA join(209125..209262,209327..209395,209985..210069, 210140..210259,210310..210468,210692..210883, 211143..211255) /locus_tag="NECAME_09025" /product="collagen triple helix repeat protein" CDS join(209125..209262,209327..209395,209985..210069, 210140..210259,210310..210468,210692..210883, 211143..211255) /locus_tag="NECAME_09025" /inference="protein motif:HMMPfam:IPR008160" /codon_start=1 /product="collagen triple helix repeat protein" /protein_id="ETN80703.1" /db_xref="InterPro:IPR008160" /translation="MTVKERAAVAANLRRLALFSSAVATTTIIVAVLIIPMLVSAAVW AQVDSERLFDPCYSSTATTLQLLDEMNVPLLSRQKRGAYVYPPTGGVTEPINSAPPVG CVMQLGPPGPPGLPGLDGFDGRDGIPGKPGFPGRNGIPESEREPCWICQKAEPGPVGA VGPQGRRGAQGEPGRNGYSPLPVPGPPGDEGPQGFVGLVGIPGPPGLDGLHDEVVRIG GPGQPGPPGPPGLSGPPGYQGAPGSPGPPSFLPGDPVPAIPTVPGGNLVQVAPSFRTL EERLKNKNLLQRFTN" assembly_gap 210972..211071 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 214835..219878 /locus_tag="NECAME_09026" mRNA join(214835..215091,215728..215925,215994..216079, 216139..216220,216284..216347,216423..216604, 216799..216949,217007..217090,217731..217877, 217987..218112,218195..218283,219512..219647, 219750..219878) /locus_tag="NECAME_09026" /product="IWS1 protein" CDS join(214835..215091,215728..215925,215994..216079, 216139..216220,216284..216347,216423..216604, 216799..216949,217007..217090,217731..217877, 217987..218112,218195..218283,219512..219647, 219750..219878) /locus_tag="NECAME_09026" /inference="protein motif:HMMPfam:IPR017923" /codon_start=1 /product="IWS1 protein" /protein_id="ETN80704.1" /db_xref="InterPro:IPR017923" /translation="MADYGFPSEALSGFAAAVDGENSSSQQYEESSTGYQHAPSPQRS ATDGSPRPEDYERVLSPRAESSVSTPSHVSAPSSPLSAHADDSGEEFNADSPNVPGSP EANNSRDQPENYNPTSPLQDFPSSSRSPQQTEGSAPQSPRYREDDGDEKSDVEAVKTG SDDDEDARKKRSAAESGGEDDEGDEEMSRKRRRIVNSDDSDNEESGSKKNLLDSDDSD DEMDEEQRKKKLGNLMVNIFGEDVDEDADASRDADGDQDDEEGDREREQAVQDEEGYE DDYGDRNKEDRDNAGFEWDFDVMLREKKAERKKKRRKRDTGIDIINDDDGIIAHMVSA MKNAAKDDRHSNTERKPALKKRKMLGDVKMMLLRMDMLEAMIDGGMMSAVSEWLAPLP DKSLPALEIRTELLKILQGFGRLDQGVLKQSGLGRAVMLLYKHPRETKENKAMAAHII REWSRPIFQLDTDFRSVSREERVQRDLDHMSTAKKRKMNGEAGPSDQQATPKEPEKTI NRARVPRPSTKDYVVRPKSNVEGEFKGERKGRGFARLDKAQRDFMERTKKMKVKRAVG VSLEGRNMAL" assembly_gap 225947..226046 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(230542..231022) /locus_tag="NECAME_09027" mRNA complement(join(230542..230598,230897..231022)) /locus_tag="NECAME_09027" /product="hypothetical protein" CDS complement(join(230542..230598,230897..231022)) /locus_tag="NECAME_09027" /codon_start=1 /product="hypothetical protein" /protein_id="ETN80705.1" /translation="MSLHDQQQHQPPMTLQFSHRSRGRNYVRTRTSDLNTVLKLVPIP WEEQRAADEAQMNKMK" assembly_gap 232775..232874 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 233707..233806 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" CONTIG join(ANCG01023324.1:1..2012,gap(660),ANCG01023325.1:1..906, gap(100),ANCG01023326.1:1..2164,gap(100),ANCG01023327.1:1..1182, gap(378),ANCG01023328.1:1..4759,gap(718),ANCG01023329.1:1..11363, gap(100),ANCG01023330.1:1..1741,gap(1042),ANCG01023331.1:1..1895, gap(100),ANCG01023332.1:1..8517,gap(192),ANCG01023333.1:1..21253, gap(1043),ANCG01023334.1:1..1154,gap(2224),ANCG01023335.1:1..1890, gap(437),ANCG01023336.1:1..3490,gap(100),ANCG01023337.1:1..1235, gap(100),ANCG01023338.1:1..3160,gap(100),ANCG01023339.1:1..3732, gap(1103),ANCG01023340.1:1..2783,gap(100),ANCG01023341.1:1..5545, gap(284),ANCG01023342.1:1..3169,gap(1420),ANCG01023343.1:1..4247, gap(368),ANCG01023344.1:1..1789,gap(110),ANCG01023345.1:1..2970, gap(2487),ANCG01059752.1:1..1255,gap(100),ANCG01023346.1:1..1980, gap(1550),ANCG01023347.1:1..1792,gap(100),ANCG01023348.1:1..1388, gap(1046),ANCG01023349.1:1..5885,gap(827),ANCG01023350.1:1..850, gap(100),ANCG01023351.1:1..1499,gap(100),ANCG01023352.1:1..623, gap(531),ANCG01023353.1:1..668,gap(289),ANCG01023354.1:1..692, gap(100),ANCG01065043.1:1..24767,gap(193),ANCG01023355.1:1..7159, gap(100),ANCG01023356.1:1..892,gap(1793),ANCG01023357.1:1..755, gap(885),ANCG01023358.1:1..1121,gap(2135),ANCG01023359.1:1..541, gap(2351),ANCG01023360.1:1..663,gap(100),ANCG01023361.1:1..994, gap(486),ANCG01023362.1:1..2017,gap(1274),ANCG01023363.1:1..1388, gap(1044),ANCG01023364.1:1..7642,gap(100),ANCG01023365.1:1..831, gap(100),ANCG01023366.1:1..2533,gap(100),ANCG01023367.1:1..4328, gap(1216),ANCG01023368.1:1..4380,gap(100),ANCG01023369.1:1..13386, gap(100),ANCG01023370.1:1..14875,gap(100),ANCG01023371.1:1..6728, gap(100),ANCG01023372.1:1..832,gap(100),ANCG01023373.1:1..3015) //