LOCUS KI658446 270725 bp DNA linear CON 23-MAR-2015 DEFINITION Necator americanus unplaced genomic scaffold N_americanus-1.0_Cont183, whole genome shotgun sequence. ACCESSION KI658446 ANCG01000000 VERSION KI658446.1 DBLINK BioProject: PRJNA72135 BioSample: SAMN02953824 KEYWORDS WGS; HIGH_QUALITY_DRAFT. SOURCE Necator americanus (New World hookworm) ORGANISM Necator americanus Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae; Bunostominae; Necator. REFERENCE 1 (bases 1 to 270725) AUTHORS Mitreva,M. TITLE Draft genome of the hookworm Necator americanus JOURNAL Unpublished REFERENCE 2 (bases 1 to 270725) AUTHORS Mitreva,M., Abubucker,S., Martin,J., Minx,P., Warren,C., Pepin,K.H., Palsikar,V.B., Zhang,X.W.E. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (17-APR-2013) The Genome Institute, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Necator americanus is a roundworm that causes most of the human hookworm infections. N. americanus is a blood-feeding nematode infecting people in the rural areas of the tropics and subtropics causing an estimated disease burden of 22 million disability adjusted life years. The life cycle of the parasite begins when parasite eggs from an infected host are passed in the feces. In favorable environmental condition they hatch, and through 2 larval stages develop to the infective stage (L3). L3s penetrate human skin, migrate through the circulatory system and lung to finally reside in duodenum. The development continues and the adult stages attach to the intestinal mucosa and feed on blood. The strain being sequenced was obtained from the laboratory of Dr. Peter Hotez by Dr. Bin Zhan, originally isolated from an infected patient in Hunan province, China, and has been maintained in hamster since 1976.The adult worms were collected from intestines of hamsters infected subcutaneously with N. americanus L3 for 8 weeks. Worm isolation and DNA extraction was performed by Bin Zhan with QIAamp DNA mini kit according to manufacturers instructions (Qiagen). Jian et. al., 2003 Exp Parasitol. This assembly consists of fragments, 3kb and 8kb insert whole genome shotgun libraries. The sequences were generating on the Roch/454 platform and assembled using Newbler. To improve scaffolding, an in-house tool CIGA (Cdna tool for Improving Genome Assembly), was used to map 454 cDNA reads using blat to the genomic assembly to link genomic contigs based on cDNA evidence. Only joins confirmed by additional independent data typing were accepted. The repeat library was generated using Repeatmodeler (A.F.A. Smit, R. Hubley & P. Green http://repeatmasker.org). The Ribosomal RNA genes were identified using RNAmmer (Lagesen et. al., 2007 Nucleic Acids Res.) and transfer RNA's were identified with tRNAscan-SE (Lowe and Eddy, Nucleic Acids Res. 1997). Non-coding RNAs, such as microRNAs, were identified by sequence homology search of the Rfam database (Griffiths-Jones et. al., 2003 Nucleic Acids Res.). Repeats and predicted RNA's were then masked using RepeatMasker (A. Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding genes were predicted using a combination of ab initio programs Snap (Korf, 2004 BCM Bioinformatics), Fgenesh (Salamov A., Solovyev V. 2000, Genome Res.) and Augustus (M. Stanke, et. al., 2008 Bioinformatics) and the annotation pipeline tool Maker (M. Yandell et. al., 2007 Genomic Research) which aligns mRNA, EST and protein information from same species or cross-species to aid in gene structure determination and modifications. A consensus gene set from the above prediction algorithms was generated, using a logical, hierarchical approach developed at the Genome institute. Gene product naming was determined by BER (http://ber.sourceforge.net). Our goal is to explore this WGS draft sequence of N. americanus to better define proteins involved in nematode parasitism that impact health and disease and are relevant to both host-parasite relationships and basic biological processes. For information regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. For specific questions regarding the N. americanus genome project contact Makedonka Mitreva (mmitreva@genome.wustl.edu) at Washington University School of Medicine. The National Human Genome Research Institute (NHGRI) of the National Institutes of Health (NIH) provided funds for this project. ##Genome-Assembly-Data-START## Finishing Goal :: High-Quality Draft Current Finishing Status :: High-Quality Draft Assembly Method :: Newbler v. MapAsmResearch-04/19/2010-patch- 08/17/2010 Assembly Name :: N_ americanus_v1 Genome Coverage :: 26.15x Sequencing Technology :: 454 ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..270725 /organism="Necator americanus" /mol_type="genomic DNA" /submitter_seqid="N_americanus-1.0_Cont183" /host="Homo sapiens" /db_xref="taxon:51031" /chromosome="Unknown" /lab_host="hamster" /geo_loc_name="China: Hunan Province" assembly_gap 2437..2842 /estimated_length=406 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 4155..6593 /estimated_length=2439 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 7402..7744 /estimated_length=343 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 8326..30163 /locus_tag="NECAME_07896" mRNA join(8326..8417,13856..13964,26836..26898,30122..30163) /locus_tag="NECAME_07896" /product="hypothetical protein" CDS join(8326..8417,13856..13964,26836..26898,30122..30163) /locus_tag="NECAME_07896" /note="KEGG: cbr:CBG02916 Cbr-clh-2; C. briggsae CBR-CLH-2 protein; K05331 chloride channel, invertebrate 2.0e-08" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82510.1" /translation="MLDPMTKVSILSPLAMLQMHHHKPKKSWIRRISEIFHNFHMREA FAKIPAKFLRPAKKRCTVRNGSQVKECLCNDPDNAFYSISWTLNAAMQFGSVRSLLL" assembly_gap 8909..9746 /estimated_length=838 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 11306..11859 /estimated_length=554 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 14011..14585 /estimated_length=575 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 15682..16335 /estimated_length=654 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 16945..17232 /estimated_length=288 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 19114..20053 /estimated_length=940 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 21010..21583 /estimated_length=574 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 22407..22635 /estimated_length=229 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 25006..25105 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 29412..29511 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 37382..37481 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 40585..40729 /estimated_length=145 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 41446..42286 /estimated_length=841 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 43833..53047 /locus_tag="NECAME_07897" mRNA join(43833..43953,45961..46080,47357..47488,47571..47740, 48564..48726,48790..48921,50480..50561,50633..50734, 51933..52017,52880..53047) /locus_tag="NECAME_07897" /product="chloride transporter, ClC family" CDS join(43833..43953,45961..46080,47357..47488,47571..47740, 48564..48726,48790..48921,50480..50561,50633..50734, 51933..52017,52880..53047) /locus_tag="NECAME_07897" /inference="protein motif:HMMPfam:IPR001807" /note="KEGG: cbr:CBG02916 Cbr-clh-2; C. briggsae CBR-CLH-2 protein; K05331 chloride channel, invertebrate 2.4e-166" /codon_start=1 /product="chloride transporter, ClC family" /protein_id="ETN82511.1" /db_xref="InterPro:IPR001807" /translation="MSDVKRSLLDMSPGGKAEDEEEPSEDELIESNETFSEFCSRQAR NVVHFLVEDWCLSALLGIITAVLSVGMDVAIELLQHAHVVLYDMCLRSSAYIAFFSWV LHVVVFTALSTVFCQIVSKQAVGSGIPEVKVIMHGFKMDNYLTFRTLIAKMVGLTLAM GGGLPIGKEVGSREVCGCLSKGPFVHIGAIVATLLSKITASCQYSAFFSNEGREMEML SSGCAVGIACTFSAPIGAVLYAIESTSKYFAVKNYWRGFLAATCSAIIFRFANFFVTA EQSGTITAFYQTRFPTDAFLIEELPVFMLLGFFSGMMGAIFIFTHRHISIFRQRNKVF KMIFKNNFLSFTVFMAMVVGILTYPLGFGRFIAGKLTFRETMADFFNNCTWYTNETSR RYAVSLEKIFVDFFFYGVFLACTWFILPLCMK" assembly_gap 49178..49353 /estimated_length=176 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 50184..50419 /estimated_length=236 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 51388..51852 /estimated_length=465 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 53581..54318 /estimated_length=738 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 57670..57769 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 61425..64679 /locus_tag="NECAME_07898" mRNA join(61425..61595,61661..61734,61806..61911,62048..62206, 62302..62370,62431..62529,62677..62819,62878..63001, 63064..63192,63260..63472,63531..63649,63710..63807, 63864..63940,64095..64160,64229..64361,64525..64679) /locus_tag="NECAME_07898" /product="DEAD/DEAH box helicase" CDS join(61425..61595,61661..61734,61806..61911,62048..62206, 62302..62370,62431..62529,62677..62819,62878..63001, 63064..63192,63260..63472,63531..63649,63710..63807, 63864..63940,64095..64160,64229..64361,64525..64679) /locus_tag="NECAME_07898" /inference="protein motif:HMMPfam:IPR001650" /inference="protein motif:HMMPfam:IPR011545" /note="KEGG: cbr:CBG07062 Hypothetical protein CBG07062; K01529 3.6e-172" /codon_start=1 /product="DEAD/DEAH box helicase" /protein_id="ETN82512.1" /db_xref="InterPro:IPR001650" /db_xref="InterPro:IPR011545" /translation="MRREKKKAEKVVDAEENFELTTTSEKKEKTKRRVRIADDLPETS GCGNSSESQSTIKKVVAEVECETQSGRKRKRNRKNKHQKALLESFKKAKLNVVEPEVG EKTFNDFSIDERILKAVGNLGWKWPTQVQESMFALALEDKNIMARARTGSGKTGAYLI PIIHKCIAYSSDSEELRGPSALFIAPTKELCVQINDLLVKLLQPLPFLRSLNLSDLTS DERSVWEHDFANFVVSTPGKILEMLAVRPQFCSDVRHLVLDEADLLLSFGYEEEMNAL KKHLPVTYQCILTSATITDDMSALKSLFMTGPVFTIKLKEGDLPDVDQLTQYQITCLD DNERFAILVSMFKLRLIVGKTIIFVSPGPIVYSELSCRTSLVLRAFGLKSCILNSSMP ANSRFHVINQYNNGACEIVIASDAVDAFGSDSVQPKEPVRRDKEAGVSRGIDFHQVGN VVNLDFPTSTDCYIHRVGRTARGRNKGTALSFCTPAERQYLDTVQEDINTQMGRKVII PYEIRIKELDSFLLRVKVKEVLSKCGKSIIKAARMQDIKLEIMRSAKLQSFFANNPRE KTLMETTTRPTTVAVHTTAIADVPDYMVPKSLRGINFSIGTKKKSKPGQRHRNKLKGK SVNQKIYNRKKNDPLKSFKI" assembly_gap 65112..66295 /estimated_length=1184 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 67570..67669 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 69691..70047 /estimated_length=357 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(72094..73396) /locus_tag="NECAME_07899" mRNA complement(join(72094..72353,73049..73132,73282..73396)) /locus_tag="NECAME_07899" /product="hypothetical protein" CDS complement(join(72094..72353,73049..73132,73282..73396)) /locus_tag="NECAME_07899" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82513.1" /translation="MDSGSIAEDPPQKQLDETRNKTDCKGSQSKATDSGTDEKKWQVA SQTPNADSDQKKQMKPSECQKLEQSGDGYENFGPPPEIAEAHAKADYEGSLIIGEQND DGGGIKKLSRVNGNKGKNTRKASSRNLDRTQDLSIQLGKKYRTRKSEIAI" gene 76704..78491 /locus_tag="NECAME_07900" mRNA join(76704..76868,76937..77134,77924..78082,78153..78338, 78399..78491) /locus_tag="NECAME_07900" /product="hypothetical protein" CDS join(76704..76868,76937..77134,77924..78082,78153..78338, 78399..78491) /locus_tag="NECAME_07900" /inference="protein motif:HMMPfam:IPR008574" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82514.1" /db_xref="InterPro:IPR008574" /translation="MSTALTDTYGNYQNDMERTLGSLRNQVAQLQQDLPKMIKDVQPN SKEILNAVKSTKRVSGPQRVVVEVFAWSSVMVLASTIGSMLGAYILGPFASLFFGKFA AALLAFVIIPVVSHATIKNKERHFESDNRIRTMLLSTSIGQGALAGYAINSLYLSAQP LAFVTPAVVSVGYTISVRQTNGDRAQILGISLGLACLTNVLLGTMLSGNTSSYQFLTL GYVGIAGVIMQLVLHDSSSRGYTYQNALNSLYLLFKGATYYCFGTYIE" assembly_gap 80284..80559 /estimated_length=276 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 81949..86261 /locus_tag="NECAME_07901" mRNA join(81949..82059,82122..82204,82276..82380,82624..82708, 83784..83914,83986..84099,84158..84237,84300..84435, 84496..85856,85921..86261) /locus_tag="NECAME_07901" /product="hypothetical protein" CDS join(81949..82059,82122..82204,82276..82380,82624..82708, 83784..83914,83986..84099,84158..84237,84300..84435, 84496..85856,85921..86261) /locus_tag="NECAME_07901" /inference="protein motif:HMMPfam:IPR017442" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82515.1" /db_xref="InterPro:IPR017442" /translation="MPGALTKPPEHPPWFKRMLFEEERPDMSSKCGKSDLENEEIELL DTFMREREDLIPGDLVIVNDNLDEDSREYSRYEVLTKYNEGRYSAIYIVAKQTCSDNN EVMDKCLYAMKVGVRKESSNTQLRFKEVHGIGYIHRDIKPSNFCIGVGTAAARLFLID FGETVKNGKNIKYGVPDAFSLPYWAIDSHKKTAASPKIDVESWFYTFVEMLYPEILTW KKCQVEPEVLSAKIKFWEEFPQSVPNCPPQIAEAAKIINGATDKLDYNELKKLIEDAK NAFLNGAPLTLEWVKEMPKTLPKRGDYLAQSGVEDKSSPKEAKAPKIKAAKESKEVVE GSGNIDEWKSAASTLMQKFRFRTRRKKISLPIAIPMKDADTSTSTSTTADATAGLATA IPPEQKKSEKPEETPSADKKPVEEPKETLFQRISFRKKKKPMKADTVQEQPKVQTATE KPEETPIVEKKGADESKETLFQRISFRKKRKPKADTVQEQAKEPTAPTEKIPEQTKSQ PPAPGEVEVDPSKKPQPSQEKGEGLLQLLSLRVRRKKTFKGTATNLEQTPRDQAQAQD GATATATATNQATVSSTTSTNVPKSDEDRKTSSSVAPGADQIQQKSFYQKVKEFKRKM RPVGPVVGSAEAKSAEKPEVAPIKTTQTTTAEKTTSGVPEKKQSAEDETKPPPKSMLQ RLLSPTRKYSSSVALPASIRCIGLDSSSGEKPSGVATKQSPSEKEKYQSGGLLTVTAP QATDAKPSAETGAASDVGASVETKEAMKPSDDGEHHTMGEMKKKMKKFWKKVVAKEEP QNEGTNEKLAKYQDPSLKEKVRAYLQKQQGSDEPTNLSADSADQSKSGKK" assembly_gap 83521..83620 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 88825..92039 /locus_tag="NECAME_07902" mRNA join(88825..88847,89723..89839,91822..91894,91920..92039) /locus_tag="NECAME_07902" /product="hypothetical protein" CDS join(88825..88847,89723..89839,91822..91894,91920..92039) /locus_tag="NECAME_07902" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82516.1" /translation="MVEEAFDNVTLEICVISPPLVTEICPPNDFMDDDLRSIFLDKHN SLRSSLALGREYKNGNLAPTAANMQKMTFLEGLCRDSQNTNPVFALDGNKRNKGVYSV HGSNSAVF" gene 92501..99193 /locus_tag="NECAME_07903" mRNA join(92501..92741,92879..93586,95032..95181,95233..95314, 95545..95620,95677..95826,96023..96133,98940..99025, 99094..99193) /locus_tag="NECAME_07903" /product="SCP-like protein" CDS join(92501..92741,92879..93586,95032..95181,95233..95314, 95545..95620,95677..95826,96023..96133,98940..99025, 99094..99193) /locus_tag="NECAME_07903" /inference="protein motif:HMMPfam:IPR014044" /codon_start=1 /product="SCP-like protein" /protein_id="ETN82517.1" /db_xref="InterPro:IPR014044" /translation="MNQAPSIKVYDCAVESSAIRSAKTCTGEWSDPSTRPGLKENYNK VKDELTTSGVAEKVSQCCILLLSSLCGWLLFRRCLFDGPPQQYKAWLRYQKMQCFLLR RLPIWRGVRFYAIANKTDVITYKNLKKLRGDVVGKDIYEIGAVCSKCPGGTTCDSVVG LCERTAASVGAIAEAGVSRPNANTTTPSTRAPEAMCTCGTVKVDSTPATKIITTRTAK ASTTPGAKSTTPRTAKASTTSTARKTTAITAKASTTRAAEITTTKSAKVSSTPVARIT ITRKAKVSTTSAAGKTTTRAPKPGSTPTARKTTTRAPKPGTVGKTTTREAKPSTTQGA EMITTTTAKANTTPAAKTSTTAESGTTPTEPLDCSDYAGMSTSLSKEVRQSFIQKHNA LRSNLARGREKNGKYELAPPAANIQKMKYDCAIEASALNHAKTCSTSLSHPSDRPGLK ENIIHIKSHDLDEVTAASKAMDKWWSELAVSGVRSDMLFTSRIRHRISNIVTHWSKMA WHYNSRLGCAIHRCKKTYSVVCHYGPGGNVVGEYIYRVGAVCSACPSGTRCDKNTGLC V" assembly_gap 93668..94806 /estimated_length=1139 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 96514..98290 /estimated_length=1777 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(100136..100794) /locus_tag="NECAME_07904" mRNA complement(join(100136..100327,100389..100532, 100588..100794)) /locus_tag="NECAME_07904" /product="linker histone H1 and H5 family protein" CDS complement(join(100136..100327,100389..100532, 100588..100794)) /locus_tag="NECAME_07904" /inference="protein motif:HMMPfam:IPR005818" /codon_start=1 /product="linker histone H1 and H5 family protein" /protein_id="ETN82518.1" /db_xref="InterPro:IPR005818" /translation="MSVAAISASSPDAPKARAPRKPRTAPEHPTYAIMINEAITQLKG KSGSSGPTILKYITQHYKLGDNVTMINGNLKRALTRGVAKGELKQLGGTGGTPSFKVV SDRAQKKTATPRVKKPVAKNATDEPKVGKKAVATKPNAEEKAKSPAIETVPKVKGKRT VKSKAVKSVKSVAKPTVTKT" gene complement(106956..108943) /locus_tag="NECAME_07905" mRNA complement(join(106956..107084,107151..107342, 107435..107584,108517..108714,108770..108943)) /locus_tag="NECAME_07905" /product="hypothetical protein" CDS complement(join(106956..107084,107151..107342, 107435..107584,108517..108714,108770..108943)) /locus_tag="NECAME_07905" /inference="protein motif:HMMPfam:IPR008574" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82519.1" /db_xref="InterPro:IPR008574" /translation="MEAVAELYGSYRQELETAMEGLRQRGQAFGESAPQYIEDTKSQI EPQTQEIIDAVQDTSAVSTPQRAVIEVFAWASVMVFFANVGVGFGSYIFGPIISIFIG KFGATLAAFVGIPLYAHYEIKHSGGSDVNIRLELLSLAVLQGVLTGFVIDSLYLSATP FAVLTPAIVTVSFASIAASAQGNRVTLLGGSVGAAVGVNFLIGLITGNLTFVYFLLTL TYAGIAAVTMQLVFKHIQGEAKGHVYQNVLSCSFIIAKGMFFLLFGSYNPEDVQVQQD RQSK" gene complement(114745..119255) /locus_tag="NECAME_07906" mRNA complement(join(114745..114891,114952..115109, 115163..115310,116042..116168,116231..116349, 116494..116645,116762..116848,118598..118682, 118866..118916,119037..119087,119208..119255)) /locus_tag="NECAME_07906" /product="hypothetical protein" CDS complement(join(114745..114891,114952..115109, 115163..115310,116042..116168,116231..116349, 116494..116645,116762..116848,118598..118682, 118866..118916,119037..119087,119208..119255)) /locus_tag="NECAME_07906" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82520.1" /translation="MAADQMGNGDKEESYRVMAADQMSNGDKEESYRVMAADQMSNGD KEKSYKSTNIMLRFFVCIGGSPLNGNGGDSEVVKGLPGLYPYFSKLRILHRDSFDPLL QILLEGQTLLNACTVVNLYALVNHHSVATTSSEVTGIACVSESKDIELHWMQRCNELL ILYCNLEPYRMYNGKLHQRYIFRNSCISRRSIAEPRTTYLDVDTRTIVVSNPFLSREM ALQDLERTMQEITSFNLCFENTLNEAVRDELQENGYNVASTTNYLIVSGWHDPIKCNV VFNLNKVKQKRRQVVPDGYTCLEQNGKIVGICAISCSRGRRCPEICSPNKVVDIPQKF RNYRLTIRFVNGMESISDEKWTEILSRVQIALKQDKNMKTFEKFSSMLEGNPFIIE" gene complement(125676..128596) /locus_tag="NECAME_07907" mRNA complement(join(125676..125939,126772..127350, 127495..127653,127730..128596)) /locus_tag="NECAME_07907" /product="dimerization domain protein, hAT family" CDS complement(join(125676..125939,126772..127350, 127495..127653,127730..128596)) /locus_tag="NECAME_07907" /inference="protein motif:HMMPfam:IPR003656" /inference="protein motif:HMMPfam:IPR008906" /codon_start=1 /product="dimerization domain protein, hAT family" /protein_id="ETN82521.1" /db_xref="InterPro:IPR003656" /db_xref="InterPro:IPR008906" /translation="MGKYSEYFIVFENMLICKTCNWAIPKPKDCTTSSLRHHLKKKHP ELYSSFKDKDERTKTERTLAAERAVALAHGLQRTCKSEPETGPGSSSTQPQQPYVNSC PPIPQTQLNVGWDPDRDHMLLVDRAITQMMCTAVLPPSFLDNPGFRNLIAVTAPRFQL KSREFFTTSALGNLYEEYYSKIKTMLDTAAFVSFSSDVRYLSEEKGSLVVITAYFIDG DMMPRFVVVAVSLVPGIPCADDVRALFSKMLNLYDVKEEKLHVLVEHSPTSDAAIVNG IKRVNGFAYKLQEAVEDGLSMLLGENDELLFRLKEIYQIWWKHDPTKHMRDCTRLCHQ SSHGNQIKKIDMCWNCLYQMLSRLDEEKNTLSFVLVENPHFADIKGTEWELVREIIDV LKPLDFALGLVKHRFFTPTSVVIPLYKVILRQLKEDKTCMKAVKEAICKKLSALMEGC ELQEELFLATYVDPRFRGAYFTADKRQGFLEKIEEMAFLPVLKPVEVVEQQRFEDFSN PFIAFRKHEPQESANNSKLEAGTKALAELEDYLSQDPAFTADPYEYWRNDVNSAKYPL IKKLALKYLSAPGISIDCQSIFSQLECITSELRNNWESEDLEKLLFLHHNILIYGF" assembly_gap 129508..129607 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 139692..141325 /locus_tag="NECAME_07908" mRNA join(139692..139801,140974..141083,141228..141325) /locus_tag="NECAME_07908" /product="hypothetical protein" CDS join(139692..139801,140974..141083,141228..141325) /locus_tag="NECAME_07908" /inference="protein motif:HMMPfam:IPR004151" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82522.1" /db_xref="InterPro:IPR004151" /translation="MLVFLLYMNVRLTQLLQGGNGRLSTRYQLIENIKILRLLVPVVV FDTTVCAVDLIGAVFFNIQPAFNKIDCSNNFNLYGMVYTNFHNLSAAHTCALQHLLKE NEN" assembly_gap 141751..141850 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 143954..144305 /estimated_length=352 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 147237..147336 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 148342..148827 /estimated_length=486 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 150937..151036 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 152070..152225 /estimated_length=156 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 156681..156896 /estimated_length=216 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 158037..158408 /estimated_length=372 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(160920..164211) /locus_tag="NECAME_07909" mRNA complement(join(160920..161054,161136..161292, 162931..163106,163581..163625,163743..163856, 164137..164211)) /locus_tag="NECAME_07909" /product="Protein-tyrosine phosphatase" CDS complement(join(160920..161054,161136..161292, 162931..163106,163581..163625,163743..163856, 164137..164211)) /locus_tag="NECAME_07909" /inference="protein motif:HMMPfam:IPR000242" /codon_start=1 /product="Protein-tyrosine phosphatase" /protein_id="ETN82523.1" /db_xref="InterPro:IPR000242" /translation="MKRRNDLTQMKEFVAQGATGRNRYRDVGCLDNRRVILKIGKVPY IHANYVATPNRPNRFICTQEKSEAILMLCNFLEQFQFPFETKVQVRMRALEVQVPGQR LHSCIHYHWLDWPDRGVPEVDLSPIALLSKLKNSCGPIIVHCSAGIGRTGSIVLIQHA LELIHRHEPLLEMRGYLIELRKQRNNSVQTEQQYLYVHQVLLQYLKRTKYMDENVSPL LEQFTKDYLQATRGF" assembly_gap 161555..161654 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(168839..170762) /locus_tag="NECAME_07910" mRNA complement(join(168839..168881,168950..169099, 169157..169251,169333..169410,169554..169681, 170399..170460,170536..170630,170697..170762)) /locus_tag="NECAME_07910" /product="hypothetical protein" CDS complement(join(168839..168881,168950..169099, 169157..169251,169333..169410,169554..169681, 170399..170460,170536..170630,170697..170762)) /locus_tag="NECAME_07910" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82524.1" /translation="MLPIALFLLLAITFTRAAPVEQDNLAFAAEVLGSVTPQQMSCLR DANYRVALVEAYTDGQFIPDSIPNAWFAVTTGMGVEIYMTPNALHSKTAKQQVDETIQ GLISKGLTIKQLWVKITDVSKWTPSIMFNDNFLGEVMAAIKAHGRKVGIITNSAAFYE ITPGLGYYTHNVKLWYGNSEPAACDRVEENQTANFNDFKSFADWTKPDAKEYCVGRKL CDITVNSNVVSKDSIWTPPS" assembly_gap 172608..172707 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 176188..180020 /locus_tag="NECAME_07911" mRNA join(176188..176262,176344..176484,177550..177663, 179417..179592,179668..179824,179886..180020) /locus_tag="NECAME_07911" /product="Protein-tyrosine phosphatase" CDS join(176188..176262,176344..176484,177550..177663, 179417..179592,179668..179824,179886..180020) /locus_tag="NECAME_07911" /inference="protein motif:HMMPfam:IPR000242" /codon_start=1 /product="Protein-tyrosine phosphatase" /protein_id="ETN82525.1" /db_xref="InterPro:IPR000242" /translation="MTAPTTQGSSEPFRRRLSKAARRAQVKVFVEETLKKGVKGLVAE FRSMKRVNDFTKMTEFLAQIPEGRNRYKDVGCLDNDRVILNIGPVPYIHANYVATPMS PKRFICTQFPFPFETKVKVMVRELEVKVPDQPAHTCMHYHWMDWPDRGVPEADLAPIS LLARLKECTTPIIVHCSAGIGRTGSIVLIEHALELLHQPAPLLEISGYLLELRKQRNN SIQTEHQYLYIHQVLLLYLKKTKFLDDSLTPFLDSFTEDYVAATKGF" gene complement(181535..182988) /locus_tag="NECAME_07912" mRNA complement(join(181535..181577,181679..181739, 182671..182809,182974..182988)) /locus_tag="NECAME_07912" /product="hypothetical protein" CDS complement(join(181535..181577,181679..181739, 182671..182809,182974..182988)) /locus_tag="NECAME_07912" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82526.1" /translation="MMSTIALIAIGFTLLQPSIGDDTYVPPPVTDIPYPTPSPCLTPA PPTAPPVYCPFCSCNPPKGCIPREMHFLCLFLCPPRDVQED" gene complement(<187689..188738) /locus_tag="NECAME_07913" mRNA complement(join(<187689..187849,188429..188489, 188646..188738)) /locus_tag="NECAME_07913" /product="hypothetical protein" CDS complement(join(<187689..187849,188429..188489, 188646..188738)) /locus_tag="NECAME_07913" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82527.1" /translation="MEPLHTLLRRTQRSHIRPLHHVQLLHHRHQFIALCALATLQKTV FHVRQILFSGASLSAHLHLLTLSSPSTRGRKRSILVFFDQRSRFFLSLFLLITWYNKQ WVA" gene complement(190531..195900) /locus_tag="NECAME_07914" mRNA complement(join(190531..190815,190903..191004, 191074..191190,191308..191446,191724..191839, 191910..192014,192155..192217,192409..192567, 193350..193406,193536..193712,193875..194411, 194544..194805,195112..195202,195810..195900)) /locus_tag="NECAME_07914" /product="methyltransferase domain protein" CDS complement(join(190531..190815,190903..191004, 191074..191190,191308..191446,191724..191839, 191910..192014,192155..192217,192409..192567, 193350..193406,193536..193712,193875..194411, 194544..194805,195112..195202,195810..195900)) /locus_tag="NECAME_07914" /inference="protein motif:HMMPfam:IPR001678" /inference="protein motif:HMMPfam:IPR013217" /codon_start=1 /product="methyltransferase domain protein" /protein_id="ETN82528.1" /db_xref="InterPro:IPR001678" /db_xref="InterPro:IPR013217" /translation="MANDILYHEVAEIIRSVLAKEQSVRKAVYGNTQMRDDAASSVRN GEETVHEEPKCNPFGTRYLTDEDRVWEHNAWDNVNWSEEMEEHARKVVEIQKTQAVDD SKAKELVNEPAKQWDAFYSQHNNNFFKDRNWLLKEFPELDMNNYSGDSTVRVLEVGCG VGNTSLPLIQWDEHRRMFLYSCDYSDVAVQVLKQNDSYDSTRICGFVWDITQEPPVDA PAKDSLDYVVCIYVLSAIHPSKVRIAIDNLVALLKPGGMLLLKDYGRFDLTQLRFKKD RYIEENLYCRGDGTLVYFFTKDELDELLRAAGLVQKANFVDKRLIVNRAKQNKKSLLR LSCETLRFRSLFDEILKDPELRQIADDPEIKGDVNLAYVLLYEFLAGAGLGRASPRLR GVIYRHTKAIHDREKALAADGRGVAAIKESGQETESASLIPRYARINTLKWTKEEAIE TLNSEDWNVIQISPEDDFAKRVGSMTEDEVLIDPHVENLLIFPHSNEFHRYWLVEQRY LILQDKASCLPAFLLDPPPGSQVFDTCAAPGMKTSHVASIIGGTGKVWAMDRSEERVG IMNQILEECGVENASVFHGDFLKTDVTDKKFSKVKYAIVDPPCSGSGMVKRLDELTGG NADKIRLEKLKNLQAMILKHALKFPKLQRAVYSTCSIHEEENEQVVDEVLLDTYVRQH FRLSPFVLPNWTHRGLGTYEFGNDCLRADPAKTLTNGFFVAVFERISESAHAEEGRIK ERKKKHGEVSDAADAIEKKDNDKKRK" assembly_gap 198970..199381 /estimated_length=412 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 200708..200807 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 202296..218235 /locus_tag="NECAME_07915" mRNA join(202296..202510,203810..203911,208823..209010, 212916..213054,214974..215030,215493..215596, 217282..217402,217931..218015,218101..218235) /locus_tag="NECAME_07915" /product="GNS1/SUR4 family protein" CDS join(202296..202510,203810..203911,208823..209010, 212916..213054,214974..215030,215493..215596, 217282..217402,217931..218015,218101..218235) /locus_tag="NECAME_07915" /inference="protein motif:HMMPfam:IPR002076" /codon_start=1 /product="GNS1/SUR4 family protein" /protein_id="ETN82529.1" /db_xref="InterPro:IPR002076" /translation="MSSHAVWTGNGHTILYAPYRYENVVGPEHFWNPSAVHAFFARHW SSSIYLALGYVAIINVMQRIMENRKPLSMRTVLLLWNGALAIFSMMGTWRFGLEFFNV LWTRPFTDSVCFSVDPTGPASFWACMFAFSKIAELGDTLFLVLRKRPVIFLHWYHHAV VLVYCWHSDVNSSRDSDDTVESLHEIHHILSILVALEQNKQQCVVLRRTKKNPHCRSS LTTTINNGDDDLIFGSGESASRPNPLEITSDNHEHILLCPLVVPPIFQQLPVELTAAG RWFIWMNYFVHSIMYTYYAIVSTGIRLPKRLSMTVTALQTTQMLIGVMISVYVLYLKL NGEVCQQSFDNLAICFAIYASFLILFSKFFNTAYLVKRQPKPAVKTD" assembly_gap 202695..203428 /estimated_length=734 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 206303..206445 /estimated_length=143 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 207309..208546 /estimated_length=1238 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 210155..210638 /estimated_length=484 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 216959..217058 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(218874..222569) /locus_tag="NECAME_07916" mRNA complement(join(218874..218908,219193..219322, 219383..219572,219651..219801,219895..219975, 220590..220670,220737..220845,221104..221259, 221641..221737,222283..222381,222436..222569)) /locus_tag="NECAME_07916" /product="hypothetical protein" CDS complement(join(218874..218908,219193..219322, 219383..219572,219651..219801,219895..219975, 220590..220670,220737..220845,221104..221259, 221641..221737,222283..222381,222436..222569)) /locus_tag="NECAME_07916" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82530.1" /translation="MNGTARVLLNFADRLNPDQIGLIIDTIQTGDLLGDTVYQLAARI RPDMGLLDDLSLSKWRNETARCQTIMKLILQSQARSDTSDLIAAVLLSPCVKLGSFVN VIDLLSDKELQEYLPSMCRIVTDRRRAPLSDLQGMISKLAGRLDIVDLPKVLESCFNR LLESPCLLEELCKGYGNDCLNHPSMASIRDRLAVEITKAVSHSDWEVRDTVVDIAAIV PCFRPMLGMPLQQYGDHLCTIDFHTGFGLCISLDGPLIPLVRFDRSCYVRAAALRCLI LDAQYYEEELPQLCENVILLDVDAEPRLVAIRYLQSTLAKNISHVFRILPKAIEDTDD EIRRIMIEMCSTLLVVEEYAADTMKELQEWTEDAQIGAAVRAVLGEPTVMRPDPVEHI LADMMNALRIRFNDTIDCLCQCLGPGTT" gene complement(225285..228609) /locus_tag="NECAME_07917" mRNA complement(join(225285..225363,225419..225495, 225550..225681,226225..226369,226641..226752, 228594..228609)) /locus_tag="NECAME_07917" /product="putative phospholipid hydroperoxide glutathione peroxidase" CDS complement(join(225285..225363,225419..225495, 225550..225681,226225..226369,226641..226752, 228594..228609)) /locus_tag="NECAME_07917" /inference="protein motif:HMMPfam:IPR000889" /note="KEGG: cbr:CBG20879 Hypothetical protein CBG20879; K00432 glutathione peroxidase 9.4e-62" /codon_start=1 /product="putative phospholipid hydroperoxide glutathione peroxidase" /protein_id="ETN82531.1" /db_xref="InterPro:IPR000889" /translation="MSVSFLTSRRLLQLSTLRMSGNTIYQFTVKDADGKDVSLEKYKG KVVVIVNVASKCGLTNSNYTQMKELLDKYKSQGLEVAAFPCNQFGGQEPACEIDIKNF VADKFKFEPDLYHKIDVNGDKADPLYKFLKKEQGGTLVDAIKWNFTKFLVNREGKVIK RYAPTTEPKDMVKDLESVLNENSTKL" gene complement(229715..231815) /locus_tag="NECAME_07918" mRNA complement(join(229715..229834,230827..230937, 231017..231088,231457..231618,231687..231815)) /locus_tag="NECAME_07918" /product="hypothetical protein" CDS complement(join(229715..229834,230827..230937, 231017..231088,231457..231618,231687..231815)) /locus_tag="NECAME_07918" /inference="protein motif:HMMPfam:IPR000504" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82532.1" /db_xref="InterPro:IPR000504" /translation="MIHNLPMSITDEKLKQICRDATGCAGVITECRIWKDTSKVDANG NPRSKGFAFVNFAEHKDALACLQKLNNNPTTFTNEKRPIVEFSIENLQAIRAKARRAA SSKGEKLTGRELSEKVRQQVKQSIGEVHASGMKVMPKFLGKKMRHKNMSKTQLRKKNL GKKKEKRKQESLASDVAAKKPKGKNKKRLTKYLTLSS" gene complement(233101..234379) /locus_tag="NECAME_07919" mRNA complement(join(233101..233158,233228..233312, 233389..233452,233725..233898,234207..234325, 234370..234379)) /locus_tag="NECAME_07919" /product="hypothetical protein" CDS complement(join(233101..233158,233228..233312, 233389..233452,233725..233898,234207..234325, 234370..234379)) /locus_tag="NECAME_07919" /inference="protein motif:HMMPfam:IPR000504" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82533.1" /db_xref="InterPro:IPR000504" /translation="MEALEKKQDAKSEQMQNGKRKRPYYKDAKLKPWRMIIRNLPFKT KKEDLQNVCSKFGVFTEIVLPASKKMPGCIAGFAFVQFKSREAAEKAKDYFNANKFQD RMVAADWALPKDTYETAAEEEREQLKKKIKLEKEDDAKDVKKVMPESSRGVQKLRSQD DNDESEDER" gene 235857..238505 /locus_tag="NECAME_07920" mRNA join(235857..235916,235993..236136,236200..236271, 236339..236420,238131..238195,238425..238505) /locus_tag="NECAME_07920" /product="hypothetical protein" CDS join(235857..235916,235993..236136,236200..236271, 236339..236420,238131..238195,238425..238505) /locus_tag="NECAME_07920" /inference="protein motif:HMMPfam:IPR008518" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82534.1" /db_xref="InterPro:IPR008518" /translation="MIVPEHISVTGEALKSVGLDNHGVVDRERLMQMMNVPDRIVLTG GDGYKGYEAEPLDVMNDHITQVGENDLKDLPNKITMRESPYLDRGDPADEPVRSENSI AIEENPLQELKLMRRQIGRISTRLYQLEDEIEQRRFRDKCQRFVKKYYASETAFADLI VLNPKIC" assembly_gap 239044..239443 /estimated_length=400 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 240062..240990 /estimated_length=929 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(241872..254407) /locus_tag="NECAME_07921" mRNA complement(join(241872..241941,242439..242569, 242631..242755,243641..243728,244540..244701, 244757..244918,245189..245362,254390..254407)) /locus_tag="NECAME_07921" /product="branched-chain-amino-acid transaminase" CDS complement(join(241872..241941,242439..242569, 242631..242755,243641..243728,244540..244701, 244757..244918,245189..245362,254390..254407)) /locus_tag="NECAME_07921" /inference="protein motif:HMMPfam:IPR001544" /inference="protein motif:HMMTigr:IPR005786" /note="KEGG: cel:K02A4.1 bcat-1; Branched Chain AminoTransferase family member (bcat-1); K00826 branched-chain amino acid aminotransferase 1.1e-97" /codon_start=1 /product="branched-chain-amino-acid transaminase" /protein_id="ETN82535.1" /db_xref="InterPro:IPR001544" /db_xref="InterPro:IPR005786" /translation="MEIQVEVLHYACELFEGMKAYRGVDNRIRLFRPELNMARMRRSA VRCALPDFDGKELLKCIRELVKIDQAWVPYQKGASLYIRPTLIGTEPSLSVTLSNEAK LFVITGPAGAYFDTFDPVSLLADPQYVRAAKGGVGAFKLGCNYAPTMKIAGDALKKGC KQVLWLAGPEQHITEVGAMNVFMYWKNEEGENELITASLETGIILPGVTRQSILELAR EMGEVKVTERDFTMDELRKALKENRVYEIFGAGTAAVVIPVDMIVYQIDDHEEKLKIP LMESTKSLMQRLFKAITDIQYGRASRPEWIVEI" assembly_gap 242840..242982 /estimated_length=143 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 246799..246989 /estimated_length=191 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 247665..248760 /estimated_length=1096 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(255525..267493) /locus_tag="NECAME_07922" mRNA complement(join(255525..255553,256404..256575, 258120..258238,258313..258444,259054..259154, 259546..259643,259670..259786,260468..260598, 261177..261251,261787..262168,262227..262357, 267457..267493)) /locus_tag="NECAME_07922" /product="hypothetical protein" CDS complement(join(255525..255553,256404..256575, 258120..258238,258313..258444,259054..259154, 259546..259643,259670..259786,260468..260598, 261177..261251,261787..262168,262227..262357, 267457..267493)) /locus_tag="NECAME_07922" /inference="protein motif:HMMPfam:IPR006954" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82536.1" /db_xref="InterPro:IPR006954" /translation="MKAPFAHLREFHTLVVITSPLPQQQTQQAPVRQFFEMDTEKLKD FWHTAMISGLIKAHSKQILRNLPYVEHVVYNLCTHDAKTTVALAKCAVRVFDLRDATQ QRIEYERRQPVVSTSNFLERHPLTRVIHGTRSSKWTGVTKFLYRDAKKKRFFHKQKRG NKRKRKRRNVENRRDDDARNDPIGNPKERLRPAMNFGKVVSEYMKKVLQFPNAPDSRF FEKMNLGIDSRTPELANNAIETMEGVLEIVNAFAIIAINKKQQNRLLDIIMDVSGAGK ALEGSGYGCGAIADYETLVRIKPEIEEVKYIKLPLVQEISRKHEKWIRARNHFTEEQK MDYEEKGYAFLNEEQLNLIYNRQEQLLHGINVTELTRMTKEQKTKRIERDIRALAALD QPSVPTWGTKHFRKRRATPPSADPELKENEERINGVLFETLQPYAFTAEVLSPHILGG AHSHEEAEHTVSEIGAHSDHIHGDEEHERAEGSHHSHEIEEETVSGLPPWIHIVNGR" assembly_gap 260205..260304 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 263275..263374 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 266494..267287 /estimated_length=794 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 267892..269354 /estimated_length=1463 /gap_type="within scaffold" /linkage_evidence="paired-ends" CONTIG join(ANCG01020194.1:1..2436,gap(406),ANCG01020195.1:1..1312, gap(2439),ANCG01020196.1:1..808,gap(343),ANCG01020197.1:1..1164, gap(838),ANCG01020198.1:1..1559,gap(554),ANCG01020199.1:1..2151, gap(575),ANCG01020200.1:1..1096,gap(654),ANCG01020201.1:1..609, gap(288),ANCG01020202.1:1..1881,gap(940),ANCG01020203.1:1..956, gap(574),ANCG01020204.1:1..823,gap(229),ANCG01020205.1:1..2370, gap(100),ANCG01020206.1:1..4306,gap(100),ANCG01020207.1:1..7870, gap(100),ANCG01020208.1:1..3103,gap(145),ANCG01020209.1:1..716, gap(841),ANCG01020210.1:1..6891,gap(176),ANCG01020211.1:1..830, gap(236),ANCG01020212.1:1..968,gap(465),ANCG01020213.1:1..1728, gap(738),ANCG01020214.1:1..3351,gap(100),ANCG01020215.1:1..7342, gap(1184),ANCG01020216.1:1..1274,gap(100),ANCG01020217.1:1..2021, gap(357),ANCG01020218.1:1..10236,gap(276),ANCG01020219.1:1..2961, gap(100),ANCG01020220.1:1..10047,gap(1139),ANCG01020221.1:1..1707, gap(1777),ANCG01020222.1:1..31217,gap(100),ANCG01020223.1:1..12143, gap(100),ANCG01020224.1:1..2103,gap(352),ANCG01020225.1:1..2931, gap(100),ANCG01020226.1:1..1005,gap(486),ANCG01020227.1:1..2109, gap(100),ANCG01020228.1:1..1033,gap(156),ANCG01020229.1:1..4455, gap(216),ANCG01020230.1:1..1140,gap(372),ANCG01020231.1:1..3146, gap(100),ANCG01020232.1:1..10953,gap(100),ANCG01020233.1:1..26262, gap(412),ANCG01020234.1:1..1326,gap(100),ANCG01020235.1:1..1887, gap(734),ANCG01000273.1:1..2874,gap(143),ANCG01020236.1:1..863, gap(1238),ANCG01000274.1:1..1608,gap(484),ANCG01020237.1:1..6320, gap(100),ANCG01020238.1:1..21985,gap(400),ANCG01020239.1:1..618, gap(929),ANCG01020240.1:1..1849,gap(143),ANCG01020241.1:1..3816, gap(191),ANCG01020242.1:1..675,gap(1096),ANCG01020243.1:1..11444, gap(100),ANCG01020244.1:1..2970,gap(100),ANCG01020245.1:1..3119, gap(794),ANCG01020246.1:1..604,gap(1463),ANCG01020247.1:1..1371) //