LOCUS KI658383 324707 bp DNA linear CON 23-MAR-2015 DEFINITION Necator americanus unplaced genomic scaffold N_americanus-1.0_Cont177, whole genome shotgun sequence. ACCESSION KI658383 ANCG01000000 VERSION KI658383.1 DBLINK BioProject: PRJNA72135 BioSample: SAMN02953824 KEYWORDS WGS; HIGH_QUALITY_DRAFT. SOURCE Necator americanus (New World hookworm) ORGANISM Necator americanus Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae; Bunostominae; Necator. REFERENCE 1 (bases 1 to 324707) AUTHORS Mitreva,M. TITLE Draft genome of the hookworm Necator americanus JOURNAL Unpublished REFERENCE 2 (bases 1 to 324707) AUTHORS Mitreva,M., Abubucker,S., Martin,J., Minx,P., Warren,C., Pepin,K.H., Palsikar,V.B., Zhang,X.W.E. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (17-APR-2013) The Genome Institute, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Necator americanus is a roundworm that causes most of the human hookworm infections. N. americanus is a blood-feeding nematode infecting people in the rural areas of the tropics and subtropics causing an estimated disease burden of 22 million disability adjusted life years. The life cycle of the parasite begins when parasite eggs from an infected host are passed in the feces. In favorable environmental condition they hatch, and through 2 larval stages develop to the infective stage (L3). L3s penetrate human skin, migrate through the circulatory system and lung to finally reside in duodenum. The development continues and the adult stages attach to the intestinal mucosa and feed on blood. The strain being sequenced was obtained from the laboratory of Dr. Peter Hotez by Dr. Bin Zhan, originally isolated from an infected patient in Hunan province, China, and has been maintained in hamster since 1976.The adult worms were collected from intestines of hamsters infected subcutaneously with N. americanus L3 for 8 weeks. Worm isolation and DNA extraction was performed by Bin Zhan with QIAamp DNA mini kit according to manufacturers instructions (Qiagen). Jian et. al., 2003 Exp Parasitol. This assembly consists of fragments, 3kb and 8kb insert whole genome shotgun libraries. The sequences were generating on the Roch/454 platform and assembled using Newbler. To improve scaffolding, an in-house tool CIGA (Cdna tool for Improving Genome Assembly), was used to map 454 cDNA reads using blat to the genomic assembly to link genomic contigs based on cDNA evidence. Only joins confirmed by additional independent data typing were accepted. The repeat library was generated using Repeatmodeler (A.F.A. Smit, R. Hubley & P. Green http://repeatmasker.org). The Ribosomal RNA genes were identified using RNAmmer (Lagesen et. al., 2007 Nucleic Acids Res.) and transfer RNA's were identified with tRNAscan-SE (Lowe and Eddy, Nucleic Acids Res. 1997). Non-coding RNAs, such as microRNAs, were identified by sequence homology search of the Rfam database (Griffiths-Jones et. al., 2003 Nucleic Acids Res.). Repeats and predicted RNA's were then masked using RepeatMasker (A. Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding genes were predicted using a combination of ab initio programs Snap (Korf, 2004 BCM Bioinformatics), Fgenesh (Salamov A., Solovyev V. 2000, Genome Res.) and Augustus (M. Stanke, et. al., 2008 Bioinformatics) and the annotation pipeline tool Maker (M. Yandell et. al., 2007 Genomic Research) which aligns mRNA, EST and protein information from same species or cross-species to aid in gene structure determination and modifications. A consensus gene set from the above prediction algorithms was generated, using a logical, hierarchical approach developed at the Genome institute. Gene product naming was determined by BER (http://ber.sourceforge.net). Our goal is to explore this WGS draft sequence of N. americanus to better define proteins involved in nematode parasitism that impact health and disease and are relevant to both host-parasite relationships and basic biological processes. For information regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. For specific questions regarding the N. americanus genome project contact Makedonka Mitreva (mmitreva@genome.wustl.edu) at Washington University School of Medicine. The National Human Genome Research Institute (NHGRI) of the National Institutes of Health (NIH) provided funds for this project. ##Genome-Assembly-Data-START## Finishing Goal :: High-Quality Draft Current Finishing Status :: High-Quality Draft Assembly Method :: Newbler v. MapAsmResearch-04/19/2010-patch- 08/17/2010 Assembly Name :: N_ americanus_v1 Genome Coverage :: 26.15x Sequencing Technology :: 454 ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..324707 /organism="Necator americanus" /mol_type="genomic DNA" /submitter_seqid="N_americanus-1.0_Cont177" /host="Homo sapiens" /db_xref="taxon:51031" /chromosome="Unknown" /lab_host="hamster" /geo_loc_name="China: Hunan Province" assembly_gap 2350..3236 /estimated_length=887 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 4070..4169 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 5070..6156 /estimated_length=1087 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 8755..11871 /estimated_length=3117 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 12751..14510 /estimated_length=1760 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 15527..16430 /estimated_length=904 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 17255..19173 /estimated_length=1919 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 19990..20089 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 20595..21649 /estimated_length=1055 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 22532..23444 /estimated_length=913 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 24283..24382 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 25660..25759 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 26403..29871 /locus_tag="NECAME_07771" mRNA join(26403..26411,26582..26638,29147..29249,29327..29406, 29788..29871) /locus_tag="NECAME_07771" /product="hypothetical protein" CDS join(26403..26411,26582..26638,29147..29249,29327..29406, 29788..29871) /locus_tag="NECAME_07771" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82766.1" /translation="MLKMLSIRSKIKKENLEICNSEVQKYSSKTSPLLRNNTNYSLGL DILMMADYATFQGFVEISNGDAHMGQAYTLEYLRAVFEQVKTIYNDIKISKQVIRLKM VGSFIALR" assembly_gap 30044..31051 /estimated_length=1008 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 32576..32723 /estimated_length=148 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 33714..34936 /locus_tag="NECAME_07772" mRNA join(33714..33780,34803..34936) /locus_tag="NECAME_07772" /product="hypothetical protein" CDS join(33714..33780,34803..34936) /locus_tag="NECAME_07772" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82767.1" /translation="MAAKMAAVTPWRWHSGQLFVESVLFREDDCPMFTTFKRNEFVNE DNATSPDNSTYDSGFEETNSTE" assembly_gap 34328..34643 /estimated_length=316 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 35043..36537 /locus_tag="NECAME_07773" mRNA join(35043..35149,35262..35411,35668..35829,35890..36049, 36105..36239,36295..36340,36413..36537) /locus_tag="NECAME_07773" /product="reprolysin family zinc metalloprotease" CDS join(35043..35149,35262..35411,35668..35829,35890..36049, 36105..36239,36295..36340,36413..36537) /locus_tag="NECAME_07773" /inference="protein motif:HMMPfam:IPR001590" /codon_start=1 /product="reprolysin family zinc metalloprotease" /protein_id="ETN82768.1" /db_xref="InterPro:IPR001590" /translation="MTLTVSAIAALDKFTYWLKVHRLFLPKHDHAILITKFDLISPRG DSGTQGMAYVGSICQPGDSASIVEDVGAAATALIAAHELGHSLGAFHDGNPQSQDCPS FANFLMAVTVSGTEDFERFAHSRVMSPCSIESIEKKLKSPLASCVRKSMPKEHQLMGM SSLSQETTPTPGELISLQQQCQITFGPHYGVCPSKDYFRGLDVCRRIWCKDRTKRRSG PCETRTYLPALDGTECGKMKWCIAGRCVDNPKKLQECIDLNPKTCGKYSKIKLRHYCK SKDFAEICCRSCAQLKDL" assembly_gap 36865..38271 /estimated_length=1407 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 39557..41130 /estimated_length=1574 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 42803..43504 /estimated_length=702 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 45305..46901 /estimated_length=1597 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 48360..49920 /estimated_length=1561 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 53231..53962 /estimated_length=732 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 61182..63787 /locus_tag="NECAME_07774" mRNA join(61182..61311,61370..61448,62662..62790,63009..63142, 63639..63787) /locus_tag="NECAME_07774" /product="hypothetical protein" CDS join(61182..61311,61370..61448,62662..62790,63009..63142, 63639..63787) /locus_tag="NECAME_07774" /inference="protein motif:HMMPfam:IPR019424" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82769.1" /db_xref="InterPro:IPR019424" /translation="MGISVSLLVLVSLITIAFNCAGLFGNFNIIIALIRKPALRSKAG YLMGLLCCFQSFCLLFELSNIRIYWSEMIMGKGVCFRVVYPHLFCMVAQSLMYFIITL DMLIAISAPLRHKMWPKVPYVIVMCAPPTMFASTAWIGSYFYIGEGSFYSCRLLTASI ISVGAAADVPPSSLSGRCFFWPVISLTMLNLSHCFHDSDCYDTSKL" assembly_gap 62455..62554 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 65280..65600 /locus_tag="NECAME_07775" mRNA 65280..65600 /locus_tag="NECAME_07775" /product="hypothetical protein" CDS 65280..65600 /locus_tag="NECAME_07775" /inference="protein motif:HMMPfam:IPR000477" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82770.1" /db_xref="InterPro:IPR000477" /translation="MDTMMKEFHKQHPWTLLFADDVVLASESRDDLQKQVQSWKDRLQ QHGLRPNISKSEYMECGPRIENGSIRVDGTELNKVNCFKYLGSKLTSTGDIDQEGRAR VNTP" assembly_gap 66015..66320 /estimated_length=306 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 67697..67796 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 69108..69423 /estimated_length=316 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 72062..72209 /estimated_length=148 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 73210..77922 /locus_tag="NECAME_07776" mRNA join(73210..73254,77559..77655,77768..77922) /locus_tag="NECAME_07776" /product="hypothetical protein" CDS join(73210..73254,77559..77655,77768..77922) /locus_tag="NECAME_07776" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82771.1" /translation="MKANTGIINTEVKEKLIRAVSIMVTIFACTWYLCVVSVYVALKL KIQDGVSVRSLFSKLLCAMVSLPTISSCVRGAVTVDEILLSSIKQRKHSYKNKC" assembly_gap 73876..74755 /estimated_length=880 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(80308..87580) /locus_tag="NECAME_07777" mRNA complement(join(80308..80443,87386..87480,87563..87580)) /locus_tag="NECAME_07777" /product="hypothetical protein" CDS complement(join(80308..80443,87386..87480,87563..87580)) /locus_tag="NECAME_07777" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82772.1" /translation="MSQYPPQYPPNQPYYAQQPGYYPQQPGCYPQGQPQTIYVQEPRR TGGGGGKGGLCAALLAGLCCGWCLENCCDDCCGCCCDC" assembly_gap 81605..82901 /estimated_length=1297 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 85696..86641 /estimated_length=946 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(87948..92306) /locus_tag="NECAME_07778" mRNA complement(join(87948..88048,92009..92306)) /locus_tag="NECAME_07778" /product="hypothetical protein" CDS complement(join(87948..88048,92009..92306)) /locus_tag="NECAME_07778" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82773.1" /translation="MISRRPGTFGALKVTRNGLSKRLVQRVMLTLKTTLTPTSRTERS LAKQSYLPDLLKTSPRCEFLEESSAEGNDEFEALYLRSTVGTFLKGRKVCIDANGGSV LFHRKQQTRSAKVVTQESEEECSVATFDSM" assembly_gap 96874..97271 /estimated_length=398 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 100165..101278 /estimated_length=1114 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 102737..103101 /estimated_length=365 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 106033..106399 /estimated_length=367 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 107236..107622 /estimated_length=387 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 111068..111772 /estimated_length=705 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 113810..115192 /estimated_length=1383 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 116082..116379 /estimated_length=298 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 119406..119505 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 120744..120843 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 120913..128527 /locus_tag="NECAME_07779" mRNA join(120913..121030,121119..121303,121361..121452, 123928..124057,126501..126654,128340..128527) /locus_tag="NECAME_07779" /product="g-protein alpha subunit" CDS join(120913..121030,121119..121303,121361..121452, 123928..124057,126501..126654,128340..128527) /locus_tag="NECAME_07779" /inference="protein motif:HMMPfam:IPR001019" /note="KEGG: cbr:CBG19263 Cbr-gpa-3; C. briggsae CBR-GPA-3 protein; K04640 guanine nucleotide binding protein (G protein), alpha, other 1.8e-83" /codon_start=1 /product="g-protein alpha subunit" /protein_id="ETN82774.1" /db_xref="InterPro:IPR001019" /translation="MGLCHSDEEKTGVEKSKAIDKQIKQGAASDERTVKLLLLGAGEC GKSTVLKQMRILHNNGFTEDEMMQQKRVVYNNTVTAIHQLIKAMQQHQIKYTSPEREF RKFVLLVKVDAMVVQDVVKQGKESEPFTPEVFDVGGQRSERKKWIHCFEDVNAIIFIA AVSEYDEVLFEDETTNRMLESQRLFESICNSRWFINTSIILFMNKKDLFMEKIKRVSI KTAFPEYTGPQTYEDSMKYIKQRFEMLNQNPKKTIFVHETCATDTDQVQKILDSVISM IIQQNLHKSGLY" assembly_gap 122533..123885 /estimated_length=1353 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 127141..128126 /estimated_length=986 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 129946..130307 /estimated_length=362 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 132247..132602 /estimated_length=356 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(134171..136549) /locus_tag="NECAME_07780" mRNA complement(join(134171..134362,134416..134544, 134658..134725,135950..136051,136324..136457, 136521..136549)) /locus_tag="NECAME_07780" /product="hypothetical protein" CDS complement(join(134171..134362,134416..134544, 134658..134725,135950..136051,136324..136457, 136521..136549)) /locus_tag="NECAME_07780" /inference="protein motif:HMMPfam:IPR019424" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82775.1" /db_xref="InterPro:IPR019424" /translation="MLIAVVFPVKHRMWSTLMYVVSMCIPPVTIAAIALIISYHRMHG VKLRACRPFTVAHEIVTAITVLLTATNTAAVVVVFILLCVVFRKEASLKGSRHRKSIQ YLAPSVTNKTVRIISSMVAVFTCTWYFYVASTLVALNLNLPEEHLDYAMTLNLIPALV AYSQNFYALHACPRYSPVLSQQKAWLRCSPENSRISVIETSLKSSRSVLADSTATSL" assembly_gap 137185..138225 /estimated_length=1041 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 140538..141036 /estimated_length=499 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 142231..143818 /estimated_length=1588 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 146061..146422 /estimated_length=362 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 147784..147883 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 149598..149697 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 152976..154871 /estimated_length=1896 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 155599..155698 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(156173..158949) /locus_tag="NECAME_07781" mRNA complement(join(156173..156232,158616..158710, 158787..158949)) /locus_tag="NECAME_07781" /product="hypothetical protein" CDS complement(join(156173..156232,158616..158710, 158787..158949)) /locus_tag="NECAME_07781" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82776.1" /translation="MLQDDKPYPPCTGHYNAAFDLSQDSTSSEKMESEKGIPNQAFYD GEKILEQNRFDKSNSGSISARKGGPSLIRSLRKESKAKPVPPITNGFCKNIGTLDAKS AEL" assembly_gap 156582..158186 /estimated_length=1605 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(160343..161619) /locus_tag="NECAME_07782" mRNA complement(join(160343..160387,160441..160546, 160607..160668,160704..160851,160911..160986, 161580..161619)) /locus_tag="NECAME_07782" /product="hypothetical protein" CDS complement(join(160343..160387,160441..160546, 160607..160668,160704..160851,160911..160986, 161580..161619)) /locus_tag="NECAME_07782" /inference="protein motif:HMMPfam:IPR001245" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82777.1" /db_xref="InterPro:IPR001245" /translation="MAARGKRDATAQTADFGLARGVYNTEYYKVYGEDFLPLRWLAPE CITDGIFSSKSDVWAFGILMYEIVGLGQMPYPSMDNSQVFKIGIVLTHVKNGGTPAKP VYCPDPLYKIMQNCWAYERDQRPTFADILSMFERLRGKIEFQYSHLSASKTNHIPP" assembly_gap 161678..162305 /estimated_length=628 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(162449..166516) /locus_tag="NECAME_07783" mRNA complement(join(162449..162710,162773..162889, 162950..163014,163072..163202,165084..165221, 165287..165388,165437..165602,165656..165774, 165866..166006,166507..166516)) /locus_tag="NECAME_07783" /product="protein tyrosine kinase" CDS complement(join(162449..162710,162773..162889, 162950..163014,163072..163202,165084..165221, 165287..165388,165437..165602,165656..165774, 165866..166006,166507..166516)) /locus_tag="NECAME_07783" /inference="protein motif:HMMPfam:IPR001245" /codon_start=1 /product="protein tyrosine kinase" /protein_id="ETN82778.1" /db_xref="InterPro:IPR001245" /translation="MTQVLINGLLSATSYDYRFVAAYSGTFSIENRVVTFKEYYYQGV QQAKTKAGVPTAPLAVEARMDEEGWIVSWKEPASDGGSPITSYAVEMRHNRSSEWEIA ERGLDGWKLWWRPAKSDRRTWEFRVRAANTEGFGAYGYSSDKIVPATASESTQSWLYV VLTVSLIAILILLTLIFLMIRARTRAAHLKKERTHDKNCITLEKIAGINHAPCQPMPL EILNEIKNLPHVRSDFIRLEKKLGTGSFGEVWEGVATRLPMRDGETRVAIKTLRHGYE EAEKMKFMKEAILMNNFDHPNIVKLLGVCLEGPKEYLILELMEGGDLLNFLKASSPTD SFPSQLSLRDVLSMLIDIGRGGAYLESNQHVHRDLAARNCLISSGTPHRHRVTKIGAL HFPNDQNETDVDEGDLLAVLRPAF" assembly_gap 164413..164656 /estimated_length=244 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(168000..170301) /locus_tag="NECAME_07784" mRNA complement(join(168000..168059,168295..168426, 170036..170104,170194..170301)) /locus_tag="NECAME_07784" /product="hypothetical protein" CDS complement(join(168000..168059,168295..168426, 170036..170104,170194..170301)) /locus_tag="NECAME_07784" /note="KEGG: cbr:CBG11320 Cbr-rol-3; C. briggsae CBR-ROL-3 protein; K05088 proto-oncogene tyrosine-protein kinase ROS 3.6e-29" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82779.1" /translation="MDGNFSPTTSDSASCLVSPCRVKIANLRPATEYKFWATSIHKSH FNSQFLEDSEAISQEASARTKDIPGTLRPDNVTGSSLLLRWNSLQAEQPPTIISVQYK EEAQVWAPPLQSTFIFTQNR" assembly_gap 169389..169701 /estimated_length=313 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(170630..171277) /locus_tag="NECAME_07785" mRNA complement(join(170630..170809,170889..171034, 171097..171277)) /locus_tag="NECAME_07785" /product="hypothetical protein" CDS complement(join(170630..170809,170889..171034, 171097..171277)) /locus_tag="NECAME_07785" /note="KEGG: cbr:CBG11320 Cbr-rol-3; C. briggsae CBR-ROL-3 protein; K05088 proto-oncogene tyrosine-protein kinase ROS 2.7e-63" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82780.1" /translation="MFPEKGLKAMSAFAIADKLFVFVTSSEMLIYGRETVIPIPIAYP PLRQILAVSQSSQPFPERSCFTLPSSAGIQFTVVNEGKTGAFLEVAKPPPQNSCHGVS LPQTHYEVYFTRKNSDKVKHIRSYTEKIHVENGILDKETDYEVTVAWLNRYSDASGIS DSKPFRTG" assembly_gap 171867..175095 /estimated_length=3229 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(175947..184123) /locus_tag="NECAME_07786" mRNA complement(join(175947..176069,176128..176202, 177398..177501,177748..177880,177974..178062, 178123..178295,178985..179070,180113..180206, 180282..180445,180531..180613,180675..180761, 183679..183790,183900..184003,184066..184123)) /locus_tag="NECAME_07786" /product="hypothetical protein" CDS complement(join(175947..176069,176128..176202, 177398..177501,177748..177880,177974..178062, 178123..178295,178985..179070,180113..180206, 180282..180445,180531..180613,180675..180761, 183679..183790,183900..184003,184066..184123)) /locus_tag="NECAME_07786" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82781.1" /translation="MSDTKAKVSWIPPVNLPFQVSGNSWRNISYEVRLVTPDTPDSPV AIKMSNETSLALTVSPGVEYTASVRVCWNSVCSAFLNVINTAFIPFKYPPIAFLKRSN EVTIVLDLLGEETQAAQIVDAFQPCCDGIMAFDNSTQILYSIDSNEGNVMFHRASEPN EVYLFASFLTIKFLTVLPSRAMLVLASSYQIISYRLTATVEHVIYSCSTVLEDCAEII GLSSDDDTGEIHFLAQFPNGTTALYELNQEDRTPHLLSTSTDLPNIRQLLISHGKVFF VTQKGRVGQCDKKLGSLNVNYALADVNAVIPLHPMTSPNRVEVQPDSIAMGDSKKDEI SWVAEPRQDPGSVLYKISLYKDKLFVGDAYTAITTLTKLTLPQDLLQSWSSAQKFDMN IAGINAWMSFNTSKTGLQAPLKPPTAPTNVRLYATQQDDATTQGGPVASTVTHYSFEV KSGKVECSVAAANEPNNIGEYTPKISIDSSGGSISWLSIFQCMK" assembly_gap 176967..177151 /estimated_length=185 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 179249..179902 /estimated_length=654 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 181515..182583 /estimated_length=1069 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 184125..184224 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(184395..188339) /locus_tag="NECAME_07787" mRNA complement(join(184395..184621,185258..185326, 185402..185505,185562..185703,186573..186731, 186809..186958,188273..188339)) /locus_tag="NECAME_07787" /product="hypothetical protein" CDS complement(join(184395..184621,185258..185326, 185402..185505,185562..185703,186573..186731, 186809..186958,188273..188339)) /locus_tag="NECAME_07787" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82782.1" /translation="MDVTTEEQGHSTSVKLEMVQAAAPLEDGGPTMAQLVLSTGTHLL QMKNADDYIVSEEPLTIPFEVPEGDSITSLVGISSNSLVVGSSRGSVWLLSLEHNDTV ISTTPTAIRQSDEEGHSATQIEYDFMQNAIYVVLSDKGIARCTLENPSCFFLPNTDSL NPTRSVAVDSINGFLYFLGADHQVYRSELLPFETVENYQLSTIVPILDIPPSSAIEID KEKFQLLAALQNVEKKSAHLGTILAKNLISGRISTKRSGEYLAVKRLLIERDRMFWCR EKCGDTPLDEMCFYSEDPQKDGSETHFSR" assembly_gap 186220..186319 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 187181..187280 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(<188865..199972) /locus_tag="NECAME_07788" mRNA complement(join(<188865..189026,189088..189153, 189229..189324,191393..191481,191534..191669, 191766..191846,199859..199972)) /locus_tag="NECAME_07788" /product="hypothetical protein" CDS complement(join(<188865..189026,189088..189153, 189229..189324,191393..191481,191534..191669, 191766..191846,199859..199972)) /locus_tag="NECAME_07788" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82783.1" /translation="MRDALFIFCIFCICGNSVATVFSNSLKTCQIHCSERNLAFPLAH GKFTWNQDELATCDYSCRVNSCHHGCRDLDEPLSKCESRCTEEGIAFDSCTQGCHAVE HAFLVQVQELLHQVSVTIDALEQSLRLRWQFPETVLSQVQEVAAADVSWYAQSRPSLA KNGWRWTPLHVTAFRNSTLSSEVHVPLDPTSQVQVRLAFVYRSRVGVSRAITYQMPLR VVPSRIELTSQLQLSNDKVAICWKGEPSMV" assembly_gap 193403..193574 /estimated_length=172 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 201974..202841 /estimated_length=868 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 204669..204768 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 206627..207295 /estimated_length=669 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(208999..215497) /locus_tag="NECAME_07789" mRNA complement(join(208999..209079,209224..209318, 209630..209728,211624..211798,211858..211926, 212549..212672,212768..212908,212961..213055, 213186..213274,215245..215405,215469..215497)) /locus_tag="NECAME_07789" /product="adenylate/guanylate cyclase catalytic domain protein" CDS complement(join(208999..209079,209224..209318, 209630..209728,211624..211798,211858..211926, 212549..212672,212768..212908,212961..213055, 213186..213274,215245..215405,215469..215497)) /locus_tag="NECAME_07789" /inference="protein motif:HMMPfam:IPR001054" /inference="protein motif:HMMPfam:IPR011645" /note="KEGG: cel:T03D8.5 gcy-22; Guanylyl CYclase family member (gcy-22); K01769 guanylate cyclase, other 9.5e-101" /codon_start=1 /product="adenylate/guanylate cyclase catalytic domain protein" /protein_id="ETN82784.1" /db_xref="InterPro:IPR001054" /db_xref="InterPro:IPR011645" /translation="MDSIDEEGDRLAPETLELIRQRGAARSEGNQEHTSELPRFCREA IKEDLKGRRAEVLAEAAEVEIVRLVTKSATNPFRPSLDSVEVVDVIPSMISLIRECWS ESPRYRPSMKAAKKQLISMQKGKKQNLMDHVMDTLENYASSLEAQVEERMKELVAEKK KSDTLLYRMLPKQVADKLKAGESVEPESYDGVTIFFSDVVSFTTLASKCTPMQVVALL NGLYTFFDERISRQDVYKVETIGDGYLCASGLPNRNGQEHIKAICDLSLELIVGLKNF RIPHLANETLNIRVGIHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGKAGHVHMS NDAREFLMGTFTGYQTACRGEVLVKGKGVMMTHWLCARGDLRIMETKESDC" assembly_gap 210308..210672 /estimated_length=365 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(215927..219321) /locus_tag="NECAME_07790" mRNA complement(join(215927..216226,219253..219321)) /locus_tag="NECAME_07790" /product="hypothetical protein" CDS complement(join(215927..216226,219253..219321)) /locus_tag="NECAME_07790" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82785.1" /translation="MDSSRDFAWGHRQHAKFRRGRVQGIETPVNAVYDTGEELFLGTC DSRGVGGAGVLVNTSKAVNIVSFEKLTSRIARLGMRRCSSTPALTVFVVYAPTSRYEE EHVEAFYMDLEKLFIEDHTF" assembly_gap 217346..217851 /estimated_length=506 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 218369..218468 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 219935..220526 /estimated_length=592 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 221195..221294 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(223216..225483) /locus_tag="NECAME_07791" mRNA complement(join(223216..223278,223862..224200, 224267..224345,224401..224518,225351..225483)) /locus_tag="NECAME_07791" /product="hypothetical protein" CDS complement(join(223216..223278,223862..224200, 224267..224345,224401..224518,225351..225483)) /locus_tag="NECAME_07791" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82786.1" /translation="MAQYAPQLHDAVLIYANVLNQTLEENKNIRDGKWIFDGSTGVYE GADCLLSFVQEHQLFVILAACVTLAFIIALIMILIVVTIRVKQKEREKLDALWRIPHY ELTRTTQKSTTQSFLSASSVNHSKIFEAKKETDKICYFYHGVDTLTAYKHLVLIRFEE ATNAEFRSVCVTDILKLLRWMEMKKALQMRQIEHDNLNRFVGISVDAGLVYSLWRFCS RGTLQGLQFIHESTMVVHGHLTSQV" assembly_gap 224758..225103 /estimated_length=346 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 225815..226548 /estimated_length=734 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 227114..227213 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(227882..230286) /locus_tag="NECAME_07792" mRNA complement(join(227882..227944,228442..228610, 228926..229058,229135..229244,229299..229397, 229496..229572,230248..230286)) /locus_tag="NECAME_07792" /product="hypothetical protein" CDS complement(join(227882..227944,228442..228610, 228926..229058,229135..229244,229299..229397, 229496..229572,230248..230286)) /locus_tag="NECAME_07792" /inference="protein motif:HMMPfam:IPR001828" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82787.1" /db_xref="InterPro:IPR001828" /translation="MLPIYVVLLSTFMHVRAQETTWERKTIRIGGLFVNENSTYTPFV GYTGSIGALYLAIDQIRKRHLLDNFDFNVTIRYDNCIEEEAVGYAVELIRDFEVDVII GPTCNVPAIAVGVIAAYYNLPNYIWGFTTANELAVVPRFPTVIIMTPNYFTLSLALLS IMEHFEWNQFAFIYSDDEDNQKCPIFVNDIQARRNLMFLLPLNVFDEITKITIEELVA PETALYMFGKI" assembly_gap 230988..231087 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(233443..234258) /locus_tag="NECAME_07793" mRNA complement(join(233443..233526,233686..233780, 234192..234258)) /locus_tag="NECAME_07793" /product="hypothetical protein" CDS complement(join(233443..233526,233686..233780, 234192..234258)) /locus_tag="NECAME_07793" /inference="protein motif:HMMPfam:IPR001054" /note="KEGG: cel:T03D8.5 gcy-22; Guanylyl CYclase family member (gcy-22); K01769 guanylate cyclase, other 3.4e-20" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82788.1" /db_xref="InterPro:IPR001054" /translation="MPRYCLFGDTVNTASRMESNGKPGRVHMSDDAKEFLMKTFNGYQ TLCRGEVLIKGKGVMTTHWLCGREDMQIMESRDEDQK" assembly_gap 234996..235455 /estimated_length=460 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 235716..237190 /locus_tag="NECAME_07794" mRNA join(235716..236963,237032..237190) /locus_tag="NECAME_07794" /product="hypothetical protein" CDS join(235716..236963,237032..237190) /locus_tag="NECAME_07794" /inference="protein motif:HMMPfam:IPR000477" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82789.1" /db_xref="InterPro:IPR000477" /translation="MLRRRDRRLLQDSKVIPTDHVAAQHHLLVMDLKISRPRKRHPRT ETQRIIWWNLKDRKEVFFASVAPSALPHPTRSVAEMWLSTSSVLRLTAENTLGKTTLG KPKVQKATWFWNEEVQAAIREKKSKYKLWWRTRQPEDRGAYLAAKREAKKAVSKAKSD RYKAVYDMLDTRESERAVYRLVRARHRSTLDMEHNKIVKGADGDVLCRSGQILERWRE YYNHLCNEEFCHPPIPTVPSVEGPVLPITAVEVSAALAKMKSNKATGPDDIPVDIWKL LGDRGSMWLATLFNKIVAEGRTPDVWQTSVTVPVWKEKGDIADCTSYRPIRLLCHTMK VFERVLEARLRKIVSVSLNHCGFVKDCSTMDAIHAVRILLEKHREKNRSVHLAFLDLE KAFDRVPHELLWMSMRSHRVPEEYAIPCTSRGSSGFIPLTSAVHTVHGHDNEGNPEAA SVDSTLCRRCHARVGVSR" gene complement(236812..240879) /locus_tag="NECAME_07795" mRNA complement(join(236812..237039,239047..239112, 239173..239241,240565..240688,240752..240879)) /locus_tag="NECAME_07795" /product="adenylate/guanylate cyclase catalytic domain protein" CDS complement(join(236812..237039,239047..239112, 239173..239241,240565..240688,240752..240879)) /locus_tag="NECAME_07795" /inference="protein motif:HMMPfam:IPR001054" /inference="protein motif:HMMPfam:IPR011645" /note="KEGG: cel:B0024.6 gcy-6; Guanylyl CYclase family member (gcy-6); K01769 guanylate cyclase, other 1.2e-48" /codon_start=1 /product="adenylate/guanylate cyclase catalytic domain protein" /protein_id="ETN82790.1" /db_xref="InterPro:IPR001054" /db_xref="InterPro:IPR011645" /translation="MDHVMNTLENYASSLEEEVEERMKELVAEKKKSDALLYRMLPKQ VADKLKLGESVEPESYEVVTIFFSDVVSFTTLASKCTPMQVVALLNGLYTFFDEMISR QDVYKVETIGDGYLCASGLPNRNGQEHGMACMFQQHIVQRWWASHKAALSTAHILLVL YATSWTSITAHVGHGRKLSRDREKQDAHCGSSLDVSPEGYGQHG" assembly_gap 237214..238780 /estimated_length=1567 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(240942..243993) /locus_tag="NECAME_07796" mRNA complement(join(240942..241040,242484..242572, 243903..243993)) /locus_tag="NECAME_07796" /product="hypothetical protein" CDS complement(join(240942..241040,242484..242572, 243903..243993)) /locus_tag="NECAME_07796" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82791.1" /translation="MFASFDVSSISQQEDVSDGILPPLRFWWCTEIVRMVSKPAKNPF RPGLDTGEVADVMSSLINLIQDCWSEAPKYRPTTNEVQKLLKSIQKGK" assembly_gap 242854..243573 /estimated_length=720 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 244151..244392 /estimated_length=242 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 245301..245871 /estimated_length=571 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(247104..249395) /locus_tag="NECAME_07797" mRNA complement(join(247104..247316,247378..247551, 247609..247687,247743..247857,248603..248696, 249075..249395)) /locus_tag="NECAME_07797" /product="hypothetical protein" CDS complement(join(247104..247316,247378..247551, 247609..247687,247743..247857,248603..248696, 249075..249395)) /locus_tag="NECAME_07797" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82792.1" /translation="MAQYAAQLHDAFLVYAHVLNESLTLNKSVRDGRWMLNNTAGVYK GKSLELPLKSVVKGILFSQGALSNVTIAFDGSRVPRFVFYGVGTDNEPEKLILIEMDR RGEDATLSLFYRPEEEQNMVWKGRARPKAVPDCGFTGANCPPSFIQQYQTYVILAACV AMLFIAAVIILIVIAIRAKQKERERLDALWKIPYYELTKSTEKDTTCSFVSGLSATNS KVLNTKKETDKICYFYYGLDALTAYKHSVLIRFDEAMNAEFRQMRRIEHDNLNRFVGI SLNAGLVYSLWRFCARGTLQVGISCDNGKALLLAFCEREFLYEYEPPSELLSLHT" assembly_gap 248735..249026 /estimated_length=292 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(250432..256414) /locus_tag="NECAME_07798" mRNA complement(join(250432..250605,250952..251082, 255685..255796,255851..255965,256048..256180, 256255..256414)) /locus_tag="NECAME_07798" /product="hypothetical protein" CDS complement(join(250432..250605,250952..251082, 255685..255796,255851..255965,256048..256180, 256255..256414)) /locus_tag="NECAME_07798" /inference="protein motif:HMMPfam:IPR001828" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82793.1" /db_xref="InterPro:IPR001828" /translation="MLQNGKLDYVISLFRFSIIVRYDDCIEREAVGHAVELIRDLDVD VIIGPTCSIPAIAVGVMTAYYNLPQYVWGFTTANELAEASRFPTVTIMTPNYFTLSLA LLSIMKHFEWDQFAFVYSGSEDNQKCPIFLTDIQKAVFQNPSFTISTVVEMRNVTTTD ITSALRGTSAQARTLGRTGNNTAYVWEDRKEVPDGRDQEALQAFKRTFMISDGSTQSL DQDNDKTSALYNFTLEVSQRMAQPPISCTNCNMSKGWEVSDVKNNGKLTQEDKRER" assembly_gap 255299..255591 /estimated_length=293 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 258639..258738 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(<258884..259027) /locus_tag="NECAME_07799" mRNA complement(join(<258884..258956,259017..259027)) /locus_tag="NECAME_07799" /product="hypothetical protein" CDS complement(join(<258884..258956,259017..259027)) /locus_tag="NECAME_07799" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82794.1" /translation="MHERHRSPTQNIIRSKNFSKFNCWNPLL" assembly_gap 259480..259829 /estimated_length=350 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 262465..267522 /locus_tag="NECAME_07800" mRNA join(262465..262543,266430..266549,266761..266808, 267258..267296,267363..267426,267507..267522) /locus_tag="NECAME_07800" /product="hypothetical protein" CDS join(262465..262543,266430..266549,266761..266808, 267258..267296,267363..267426,267507..267522) /locus_tag="NECAME_07800" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82795.1" /translation="MIEGDFGKVAKFDTKFEVSIKAVTLNTELMNRGILIAFLLISSL VTVESGLGRSLKKRLKSIGRPFERGAKQAAEIAAAGAVAKVAAGALSGRRKRSEDEGA YGNVEENSLKGLMQWDGEK" assembly_gap 265437..265843 /estimated_length=407 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 267955..269883 /estimated_length=1929 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 271989..272121 /estimated_length=133 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 273556..275171 /estimated_length=1616 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 276118..277584 /locus_tag="NECAME_07801" mRNA join(276118..276229,277315..277350,277419..277482, 277569..277584) /locus_tag="NECAME_07801" /product="hypothetical protein" CDS join(276118..276229,277315..277350,277419..277482, 277569..277584) /locus_tag="NECAME_07801" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82796.1" /translation="MNRGILLAFLLISSLVTVESGLGRSLKKRLKSIGRPFAKAAVAV VGRRKRSADEEVYGNLKESSLTGWKQWDDKY" assembly_gap 276311..277036 /estimated_length=726 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 278302..279529 /estimated_length=1228 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 280883..282470 /estimated_length=1588 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 283667..284965 /estimated_length=1299 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 286596..287714 /locus_tag="NECAME_07802" mRNA join(286596..286707,286918..286965,287555..287590, 287659..287714) /locus_tag="NECAME_07802" /product="50S ribosomal protein L21 domain protein" CDS join(286596..286707,286918..286965,287555..287590, 287659..287714) /locus_tag="NECAME_07802" /codon_start=1 /product="50S ribosomal protein L21 domain protein" /protein_id="ETN82797.1" /translation="MNRGILLVFLLIIAAVSVEGGLGSKLKDHVKSIGKPFEKGAKQG AEVAAAAAVAKAVAAVMGRRKRSEDERSYKNLEESSLTE" assembly_gap 291637..291736 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 292399..292498 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 300259..300358 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 303812..303911 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene 304923..309681 /locus_tag="NECAME_07803" mRNA join(304923..304976,309523..309681) /locus_tag="NECAME_07803" /product="hypothetical protein" CDS join(304923..304976,309523..309681) /locus_tag="NECAME_07803" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82798.1" /translation="MFCSLYTFIAGLQWRLFKGCCESHALLSDVVDVAVLTNERIAEN PRRTETSEIEWEYGEQALIGASLKLT" assembly_gap 305022..305504 /estimated_length=483 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 307777..307876 /estimated_length=100 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(309511..311811) /locus_tag="NECAME_07804" mRNA complement(join(309511..309666,309739..309852, 310292..310420,310482..310642,310718..310805, 310948..311031,311109..311238,311744..311811)) /locus_tag="NECAME_07804" /product="eukaryotic aspartyl protease" CDS complement(join(309511..309666,309739..309852, 310292..310420,310482..310642,310718..310805, 310948..311031,311109..311238,311744..311811)) /locus_tag="NECAME_07804" /inference="protein motif:HMMPfam:IPR001461" /codon_start=1 /product="eukaryotic aspartyl protease" /protein_id="ETN82799.1" /db_xref="InterPro:IPR001461" /translation="MKAAYRKTDDPGNVPSGKGIDHNASCNKKRKFVASSSSTFVKST KTWTIQYGSGDAKGVLGTDTVRFGGPTEKQLVVPTTTFGLATHISSDFKNDPTDGILG LAFTSLAVDRVVPPLINAINQNLLDQPLFTVWMEHRGKLEGAVGGLFTYGAIDTTNCG PVIAYQPLSSATYFEFQMAAIGLGSYTNSKAYSVISDTGTSFIGGPKTVTDAIAKAAG AKYSALEESYTISCTAKPATLDITIGTQKYSIDPVNYIVSAGTNQCLFAIFPFNFGGF GPSWILGDPFIRQYCNIYDIGQKRMGFAASLQK" gene complement(317001..317849) /locus_tag="NECAME_07805" mRNA complement(join(317001..317055,317592..317713, 317751..317849)) /locus_tag="NECAME_07805" /product="hypothetical protein" CDS complement(join(317001..317055,317592..317713, 317751..317849)) /locus_tag="NECAME_07805" /inference="protein motif:HMMPfam:IPR001461" /note="KEGG: rno:171293 Ctsd; cathepsin D; K01379 cathepsin D 2.7e-07" /codon_start=1 /product="hypothetical protein" /protein_id="ETN82800.1" /db_xref="InterPro:IPR001461" /translation="MIERGVYAQYLQHMNAMRAQTPGVVPNRVGLLKVLDYSDYEYVA NITIGTPGQPFVIVPDTGSANLWVPASNCEMKPNAHLLPSVKSEKAF" assembly_gap 318828..319653 /estimated_length=826 /gap_type="within scaffold" /linkage_evidence="paired-ends" assembly_gap 320468..321710 /estimated_length=1243 /gap_type="within scaffold" /linkage_evidence="paired-ends" gene complement(322077..324201) /locus_tag="NECAME_07806" mRNA complement(join(322077..322147,322202..322333, 323825..323917,323990..324062,324142..324201)) /locus_tag="NECAME_07806" /product="glutathione S-transferase protein" CDS complement(join(322077..322147,322202..322333, 323825..323917,323990..324062,324142..324201)) /locus_tag="NECAME_07806" /inference="protein motif:HMMPfam:IPR004045" /note="KEGG: cel:F11G11.2 gst-7; Glutathione S-Transferase family member (gst-7); K00799 glutathione S-transferase 7.0e-34" /codon_start=1 /product="glutathione S-transferase protein" /protein_id="ETN82801.1" /db_xref="InterPro:IPR004045" /translation="MVHYKLTYFPIRGRAECARQIFALAGREYEDVRLTHEQFAAARP NLPFGQLPVLEVDGKQLSQSLAINRFLARQFGFAGKTAFEEALVDSLADQFADYLTEV KPYFLVVFGITKTDNLVREIEERRIVAWTCKVASFRHQLP" CONTIG join(ANCG01019796.1:1..2349,gap(887),ANCG01019797.1:1..833, gap(100),ANCG01019798.1:1..900,gap(1087),ANCG01019799.1:1..2598, gap(3117),ANCG01019800.1:1..879,gap(1760),ANCG01019801.1:1..1016, gap(904),ANCG01019802.1:1..824,gap(1919),ANCG01019803.1:1..816, gap(100),ANCG01019804.1:1..505,gap(1055),ANCG01019805.1:1..882, gap(913),ANCG01019806.1:1..838,gap(100),ANCG01019807.1:1..1277, gap(100),ANCG01019808.1:1..4284,gap(1008),ANCG01019809.1:1..1524, gap(148),ANCG01019810.1:1..1604,gap(316),ANCG01000263.1:1..2221, gap(1407),ANCG01019811.1:1..1285,gap(1574),ANCG01000264.1:1..1672, gap(702),ANCG01019812.1:1..1800,gap(1597),ANCG01019813.1:1..1458, gap(1561),ANCG01019814.1:1..3310,gap(732),ANCG01019815.1:1..8492, gap(100),ANCG01019816.1:1..3460,gap(306),ANCG01019817.1:1..1376, gap(100),ANCG01019818.1:1..1311,gap(316),ANCG01065024.1:1..2638, gap(148),ANCG01019819.1:1..1666,gap(880),ANCG01019820.1:1..6849, gap(1297),ANCG01000265.1:1..2794,gap(946),ANCG01019821.1:1..10232, gap(398),ANCG01019822.1:1..2893,gap(1114),ANCG01019823.1:1..1458, gap(365),ANCG01019824.1:1..2931,gap(367),ANCG01019825.1:1..836, gap(387),ANCG01019826.1:1..3445,gap(705),ANCG01019827.1:1..2037, gap(1383),ANCG01019828.1:1..889,gap(298),ANCG01019829.1:1..3026, gap(100),ANCG01019830.1:1..1238,gap(100),ANCG01019831.1:1..1689, gap(1353),ANCG01019832.1:1..3255,gap(986),ANCG01019833.1:1..1819, gap(362),ANCG01019834.1:1..1939,gap(356),ANCG01000266.1:1..4582, gap(1041),ANCG01019835.1:1..2312,gap(499),ANCG01019836.1:1..1194, gap(1588),ANCG01019837.1:1..2242,gap(362),ANCG01019838.1:1..1361, gap(100),ANCG01019839.1:1..1714,gap(100),ANCG01019840.1:1..3278, gap(1896),ANCG01019841.1:1..727,gap(100),ANCG01019842.1:1..883, gap(1605),ANCG01019843.1:1..3491,gap(628),ANCG01019844.1:1..2107, gap(244),ANCG01019845.1:1..4732,gap(313),ANCG01019846.1:1..2165, gap(3229),ANCG01019847.1:1..1871,gap(185),ANCG01019848.1:1..2097, gap(654),ANCG01019849.1:1..1612,gap(1069),ANCG01019850.1:1..1541, gap(100),ANCG01019851.1:1..1995,gap(100),ANCG01019852.1:1..861, gap(100),ANCG01019853.1:1..6122,gap(172),ANCG01019854.1:1..8399, gap(868),ANCG01019855.1:1..1827,gap(100),ANCG01019856.1:1..1858, gap(669),ANCG01019857.1:1..3012,gap(365),ANCG01019858.1:1..6673, gap(506),ANCG01019859.1:1..517,gap(100),ANCG01019860.1:1..1466, gap(592),ANCG01019861.1:1..668,gap(100),ANCG01019862.1:1..3463, gap(346),ANCG01019863.1:1..711,gap(734),ANCG01019864.1:1..565, gap(100),ANCG01019865.1:1..3774,gap(100),ANCG01019866.1:1..3908, gap(460),ANCG01019867.1:1..1758,gap(1567),ANCG01019868.1:1..4073, gap(720),ANCG01019869.1:1..577,gap(242),ANCG01019870.1:1..908, gap(571),ANCG01019871.1:1..2863,gap(292),ANCG01019872.1:1..6272, gap(293),ANCG01000267.1:1..3047,gap(100),ANCG01019873.1:1..741, gap(350),ANCG01019874.1:1..5607,gap(407),ANCG01019875.1:1..2111, gap(1929),ANCG01019876.1:1..2105,gap(133),ANCG01019877.1:1..1434, gap(1616),ANCG01019878.1:1..1139,gap(726),ANCG01019879.1:1..1265, gap(1228),ANCG01019880.1:1..1353,gap(1588),ANCG01019881.1:1..1196, gap(1299),ANCG01019882.1:1..6671,gap(100),ANCG01019883.1:1..662, gap(100),ANCG01019884.1:1..7760,gap(100),ANCG01019885.1:1..3453, gap(100),ANCG01019886.1:1..1110,gap(483),ANCG01019887.1:1..2272, gap(100),ANCG01019888.1:1..10951,gap(826),ANCG01019889.1:1..814, gap(1243),ANCG01019890.1:1..2997) //