LOCUS       KF574084                1452 bp    mRNA    linear   PLN 07-DEC-2013
DEFINITION  Malus domestica cultivar Golden Delicious glycosyltransferase
            UGT71A15 mRNA, complete cds.
ACCESSION   KF574084
VERSION     KF574084.1
KEYWORDS    .
SOURCE      Malus domestica (apple)
  ORGANISM  Malus domestica
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae;
            Maleae; Malus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Xu,Y.
  TITLE     Cloning and Bioinformatic Analysis of Phloridzin
            Glycosyltransferase from Malus domestica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Xu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2013) College of Food Science and Engineering,
            Northwest Agriculture & Forestry University, Xinonglu, Yangling,
            Shaanxi 712100, China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Malus domestica"
                     /mol_type="mRNA"
                     /cultivar="Golden Delicious"
                     /db_xref="taxon:3750"
                     /PCR_primers="fwd_seq: atgaagagaccagcacaactagtg, rev_seq:
                     ttaaattttatcaataaaacgccc"
     CDS             1..1452
                     /EC_number="2.4.1.1"
                     /function="catalyzes the transfer of phloretin to
                     phloridzin"
                     /note="gdUGT71A15"
                     /codon_start=1
                     /product="glycosyltransferase UGT71A15"
                     /protein_id="AHB33773.1"
                     /translation="MGDVIVLYASPGMGHIVSMVELGKFIVHRYGPHKFSITILYTCG
                     SIVDTASIPVYIRRISHSHPFISFRQFPRVTNNITRNISVPAITFDFIRQNDPHVRSA
                     LQEISKSATVRAFIIDLFCTSALPIGKEFNIPTYYFCTSGAAILAAFLYLPKIDEQTK
                     TTESFKDLRDTVFEFPGWKSPLKATHMVQLVLDRNDPAYSDMIYFCSHLPKSNGIIVN
                     TFEELEPPSVLQAIAGGLCVPDGPTPPVYYVGPLIEEEKELSKDADAAEKEDCLSWLD
                     KQPSRSVLFLCFGSMGSFPAAQLKEIANGLEASGQRFLWVVKKPPVEEKSKQVHGVDD
                     FDLKGVLPEGFLERTADRGMVVKSWAPQVVVLKKESVGGFVTHCGWNSVLEAVVAGVP
                     MIAWPLYAEQHMNRNVLVTDMEIAIGVEQRDEEGGFVSGEEVERRVRELMESEGGRVL
                     RERCKKLGEMASAALGETGSSTRNLVNFVSSIT"
BASE COUNT          340 a          362 c          415 g          335 t
ORIGIN      
        1 atgggagacg tcattgtact gtacgcatct ccggggatgg ggcatatcgt ctccatggtg
       61 gagctgggca agttcattgt ccaccgctac ggcccccaca aattctccat caccattctc
      121 tacacctgcg gcagcattgt cgacaccgct agcatccccg tctacatccg ccgcatctcc
      181 cactcccacc ctttcatttc cttccgccaa ttccctcgcg tcaccaataa tattacccga
      241 aacataagcg tccccgcaat cacgttcgac ttcatccgcc agaacgatcc tcatgtccgc
      301 agtgccctcc aagaaatctc taaatccgcc accgttcgcg ccttcatcat cgacctcttc
      361 tgcacctccg ctcttcccat agggaaggaa ttcaacatcc caacatacta cttctgcact
      421 tctggtgccg caattcttgc tgcttttttg tatttgccca agatcgatga gcaaaccaaa
      481 accaccgaga gtttcaaaga cctccgcgac accgttttcg aattccccgg atggaagtct
      541 cctctgaagg ctacacacat ggtccaactg gtgctcgacc ggaacgaccc tgcttattcg
      601 gacatgatct atttctgctc acatcttccc aaatccaacg gaatcatcgt caacacgttc
      661 gaagagctcg agccacctag cgtcctccag gccattgctg gaggcctgtg tgttcctgat
      721 gggccaactc cgcccgtgta ctacgttggt ccattgattg aggaagagaa agaattgagt
      781 aaggatgcag atgcggccga gaaggaggac tgcttgtcat ggctcgataa gcagccaagt
      841 cgaagcgtgc tgtttctctg tttcggaagc atgggatcat ttccggctgc tcaactgaag
      901 gagatagcga acgggttgga ggcgagcggg cagaggttcc tgtgggtggt gaagaagccg
      961 ccggttgaag agaaatcaaa gcaggtccat ggagttgacg actttgattt gaagggtgtg
     1021 ttgccagaag ggtttttgga gaggacggca gacaggggga tggtagtgaa gtcatgggcg
     1081 ccgcaggtgg tggtgttgaa gaaggagtcg gttggtgggt tcgtgacaca ttgcggatgg
     1141 aactcggtac tggaagcagt ggttgcgggg gtgccgatga ttgcttggcc gctttacgcg
     1201 gagcagcata tgaacaggaa tgttctagtg acggacatgg aaatcgcgat cggggtggag
     1261 cagagagacg aggaaggtgg gttcgtgagc ggggaagaag tggagaggag agtgagggag
     1321 ttgatggagt cggaaggagg aagagtgctt agagagaggt gcaagaaact tggggagatg
     1381 gcttcggctg ctttgggaga gaccggttcg tccaccagaa acttggtcaa ctttgttagt
     1441 agcattacat aa
//