LOCUS       KF478773                1103 bp    DNA     linear   PLN 07-MAY-2014
DEFINITION  Echinochloa crus-galli clone Alu20-C2-7_20 antimicrobial peptide
            precursor, gene, partial cds.
ACCESSION   KF478773
VERSION     KF478773.1
KEYWORDS    .
SOURCE      Echinochloa crus-galli
  ORGANISM  Echinochloa crus-galli
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD
            clade; Panicoideae; Panicodae; Paniceae; Boivinellinae;
            Echinochloa.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ryazantsev,D.Y., Rogozhin,E.A., Dimitrieva,T.V., Drobyazina,P.E.,
            Khadeeva,N.V., Egorov,T.A., Grishin,E.V. and Zavriev,S.K.
  TITLE     A novel hairpin-like antimicrobial peptide from barnyard grass
            (Echinochloa crusgalli L.) seeds: Structure-functional and
            molecular-genetics characterization
  JOURNAL   Biochimie 99, 63-70 (2014)
   PUBMED   24275143
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Ryazantsev,D.Y., Dimitrieva,T.V. and Zavriev,S.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2013) Group of Molecular Diagnostics, IBCH RAS,
            Miklukho-Maklaya, 16/10, Moscow 117997, Russia
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Echinochloa crus-galli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:90397"
                     /clone="Alu20-C2-7_20"
     mRNA            <24..>1103
                     /product="antimicrobial peptide precursor"
     CDS             24..>1103
                     /codon_start=1
                     /product="antimicrobial peptide precursor"
                     /protein_id="AHB61270.1"
                     /translation="MGKGSSGGGAWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
                     XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDGSGRGSCGSQCM
                     RRHEDEPWRVQGCVSQCRRRRGGGDDDVAVDACEGADRCRERCERRHRGDWQGKQRCL
                     MECRRREQEEDVDDRCPCPCRRQCERHGDPATRQRCVEACQRRREEERRHGGGDADEE
                     GSRGGRCERKCRRHSDWQTRQRCLQQCEQRERQGEGGRDDAGDGKDTYCADRCQSMCR
                     QRHRGDREMQRRCARKCEREEGCPRRRDATADADEAEEDDGNDRCSQRCQHHRDPDRK
                     QQCMRECRRHQGRGDDDDTGADEDDS"
     sig_peptide     24..56
     mat_peptide     294..404
                     /product="antimicrobial peptide EcAMP1-like protein"
     mat_peptide     432..536
                     /product="antimicrobial peptide EcAMP3"
     mat_peptide     978..1070
                     /product="antimicrobial peptide EcAMP2-like protein"
     gap             57..290
                     /estimated_length=234
BASE COUNT          144 a          292 c          375 g           58 t
ORIGIN      
        1 agctaggggc gagggcggcg gccatgggga agggtagtag tgggggcggc gcgtggnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacggctccg
      301 gcaggggctc gtgcgggagc cagtgcatgc gccgccacga ggacgagccg tggcgggtcc
      361 aggggtgcgt cagccagtgc cgccgccgtc gtggcggcgg cgacgacgac gtcgccgtcg
      421 acgcgtgtga gggcgccgac aggtgccggg agcggtgcga gcgccgccac cgcggggact
      481 ggcaggggaa gcagcgctgc ctgatggagt gccgccgccg cgagcaggag gaggacgtcg
      541 acgaccggtg cccgtgcccg tgccggaggc agtgcgagcg ccacggcgac ccggccacca
      601 ggcagcgctg cgtcgaggcc tgccagcgcc gccgagagga ggagcgccgc cacggcggcg
      661 gcgacgccga cgaggagggc agccgcggcg gccggtgcga gaggaagtgc cggcgccaca
      721 gcgactggca gacgaggcag cggtgcctgc agcagtgtga gcagcgcgag cgccaggggg
      781 agggcggccg cgacgacgcc ggcgacggca aggacaccta ctgcgccgac cggtgccaga
      841 gcatgtgccg gcagcgccac cgcggcgacc gggagatgca gcgccggtgc gcgaggaagt
      901 gcgagcgcga ggagggctgc ccccgccgcc gcgacgccac cgccgacgcc gacgaggccg
      961 aggaggacga cggcaacgac cgctgcagcc agcggtgcca gcaccaccgc gaccccgaca
     1021 ggaagcagca gtgcatgcgc gagtgccgcc gccaccaggg ccgcggcgac gacgacgaca
     1081 ccggcgccga cgaggacgac agc
//