LOCUS       KC493618                1692 bp    mRNA    linear   INV 10-MAR-2013
DEFINITION  Rhipicephalus microplus isolate Sikar acetylcholinesterase 2
            (AChE2) mRNA, complete cds.
ACCESSION   KC493618
VERSION     KC493618.1
KEYWORDS    .
SOURCE      Rhipicephalus microplus (Boophilus microplus)
  ORGANISM  Rhipicephalus microplus
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
            Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae;
            Rhipicephalinae; Rhipicephalus; Boophilus.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Nagar,G., Kumar,S., Ray,D.D. and Ghosh,S.
  TITLE     Molecular characterization of AChE2 mediated organophosphate
            resistance in Indian isolates of Rhipicephalus (Boophilus)
            microplus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Nagar,G., Kumar,S., Ray,D.D. and Ghosh,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2013) Division of Parasitology, Indian Veterinary
            Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122,
            India
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Rhipicephalus microplus"
                     /mol_type="mRNA"
                     /isolate="Sikar"
                     /db_xref="taxon:6941"
                     /geo_loc_name="India: Rajasthan"
                     /PCR_primers="fwd_name: bm ach e2 f, fwd_seq:
                     ccgcgtcgacatgtacgtgcgggtgtctctcgt, rev_name: bm ach e2 r,
                     rev_seq: cgccaagctttcagtatagtttcagttctttg"
     gene            1..1692
                     /gene="AChE2"
     CDS             1..1692
                     /gene="AChE2"
                     /EC_number="3.1.1.7"
                     /function="hydrolysis of acetylcholine at synapse"
                     /codon_start=1
                     /product="acetylcholinesterase 2"
                     /protein_id="AGG54058.1"
                     /translation="MYVRVSLVFASAWIIACLGYTETREAKDIAALEDGASPVVQIRA
                     GKLRGAKRVVLGEKFAYAFTGVPYAKPPVGELRYRKPESAEPWVEEVRDATVTPPSCM
                     QGNVFSPRNLLWLPYDQQKSEDCLYLNVWTPRLNTSAGLPVMAWIHGGGFQEGSAAIP
                     LDDGTYLAAFGNVVVVTIAYRLQSFGFLYDETSAPGNMGLHDQQLALKWIQENIAAFG
                     GNPGEVTLFEWSAGGISTGFHLISPGSQTLFKRAIVQSAAVTKKGRARDKTEMLEYSQ
                     KFAANFGCYGGDSAANASQDIAACMRTINASLIVAVEATFVGSGSGKFEPIYGDEFLP
                     IEPRMADFPGDKDVMIGQTANEGSNILYTTFRDTFSEALPPRKINKAEMIHFLGSLYK
                     LSLPDTEKLQKEYMGEIGDYDYDALRQALAETKGDTHVKCGAINTACKLANATANAQS
                     GKEVHFYELNYVSACVKKQPWFGMTHGDELPLVFGRVFERQGGCAGDMDYSRNIMKLW
                     SDFAKGRPPVGFQGKEWPKFTADSRSFMKLTATGSEVSTFNNEPRCKILKELKLY"
BASE COUNT          444 a          401 c          477 g          370 t
ORIGIN      
        1 atgtacgtgc gggtgtctct cgtgttcgcc tcggcatgga ttatcgcgtg tctcggctac
       61 accgagactc gagaagcaaa ggacattgct gctctggaag acggagcatc tccggtcgta
      121 cagattcgcg ctgggaaatt acgtggtgcg aagcgcgtag ttttaggcga gaagttcgcg
      181 tatgccttca ctggagtgcc atatgcgaaa ccaccggtgg gcgagctgcg ctatcgaaaa
      241 ccggagtccg cagaaccatg ggtcgaggaa gtgagggatg ccacggtgac gcccccctcg
      301 tgcatgcagg gcaacgtgtt ttcgccgaga aacctgttgt ggctgccata cgatcagcaa
      361 aaaagcgagg actgccttta cttgaacgta tggacaccga ggctgaacac gtcagcagga
      421 ctcccagtga tggcctggat tcacggaggt ggcttccaag aaggatctgc cgcaatacct
      481 ctcgatgacg gaacttattt ggctgctttt ggaaacgttg tggtggtgac catcgcatac
      541 aggttgcagt cattcggttt cctgtatgat gaaacatcgg cccccggaaa catgggcttg
      601 cacgaccagc agctggcact gaaatggatc caggaaaaca tcgctgcatt tggcggtaac
      661 cctggtgaag tcacgctgtt tgaatggagc gccggtggca tttcgacagg atttcatctt
      721 atttctcccg gaagccagac tctttttaag cgggccattg tccagagtgc agccgtgacg
      781 aagaaagggc gtgccagaga caagaccgaa atgctcgaat actcccaaaa gtttgctgct
      841 aacttcggct gctatggtgg tgactcggct gccaacgctt ctcaggatat tgccgcttgc
      901 atgaggacaa taaacgcttc tctaattgtg gcagtagaag caacgttcgt gggatcggga
      961 agcggaaagt ttgagccaat ctatggagat gagttcttgc ccatagagcc tcgcatggcc
     1021 gacttcccag gtgacaagga tgttatgatt ggtcagacag ccaatgaagg aagcaacatt
     1081 ctctacacca cgttcaggga cacgttttct gaggccctac ctcccaggaa aatcaacaag
     1141 gcagaaatga tacatttcct gggcagtctg tacaaactgt cactgccaga cacagaaaag
     1201 ctccagaaag aatacatggg tgagattggg gattacgatt acgacgctct tcggcaggcc
     1261 ctggcggaaa ccaagggaga tactcatgtg aagtgcggag ccatcaacac ggcgtgcaag
     1321 ttggccaacg caacagcgaa cgcacaatca gggaaagaag ttcactttta tgagcttaac
     1381 tacgtgtcag cttgcgtcaa gaagcagcca tggttcggga tgacccatgg cgacgagctt
     1441 ccgttggtat ttggcagagt gttcgaaagg caaggtggat gtgccggtga tatggactac
     1501 agtagaaata tcatgaagct ctggagtgat tttgccaaag gaaggccacc tgtcggattc
     1561 caaggcaaag agtggccaaa gttcacagcc gattcaaggt cattcatgaa gctgacagca
     1621 acgggatcag aagtgtctac cttcaacaat gagccacgat gcaagatact caaagaactg
     1681 aaactatact ga
//