LOCUS       ECOTNAA                 2083 bp    DNA     linear   BCT 26-APR-1993
DEFINITION  E.coli tnaA gene coding for tryptophanase and 5' flank.
ACCESSION   K00032
VERSION     K00032.1
KEYWORDS    tnaA gene; tryptophan permease; tryptophanase.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Deeley,M.C. and Yanofsky,C.
  TITLE     Nucleotide sequence of the structural gene for tryptophanase of
            Escherichia coli K-12
  JOURNAL   J. Bacteriol. 147 (3), 787-796 (1981)
   PUBMED   6268608
REFERENCE   2  (bases 267 to 268; 295 to 295)
  AUTHORS   Deeley,M.C. and Yanofsky,C.
  TITLE     Transcription initiation at the tryptophanase promoter of
            Escherichia coli K-12
  JOURNAL   J. Bacteriol. 151 (2), 942-951 (1982)
   PUBMED   6284718
COMMENT     Original source text: Escherichia coli K12 DNA.
            The identification of the incomplete ORF as low affinity tryptophan
            permease is tentative [1].
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     mRNA            223..>1954
                     /note="tnaA mRNA (5' end approx)"
     gene            539..1954
                     /gene="tnaA"
     CDS             539..1954
                     /gene="tnaA"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="AAA24676.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPATGLACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     CDS             2045..>2083
                     /note="low affinity tryptophan permease (putative);
                     putative"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24677.1"
                     /translation="MTVQAEKKHSAFW"
BASE COUNT          577 a          475 c          483 g          548 t
ORIGIN      157 bp upstream of HinfI site.
        1 gtaaaccgcg catacagccg cattctgact gtcagatgcg cttcgcttca ttgttaccgc
       61 tcctgttatt cctcaaccct ttttttaaac attaaaattc ttacgtaatt tataatcttt
      121 aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat ttaaacaatt
      181 tcagaataga caaaaactct gagtgtaata atgtagcctc gtgtcttgcg aggataagtg
      241 cattatgaat atcttacata tatgtgtgac ctcaaaatgg ttcaatattg acaacaaaat
      301 tgtcgatcac cgcccttgat ttgcccttct gtagccatca ccagagccaa accgattgat
      361 tcaatgtgtt ctatttgttt gctatatctt aattttgcct tttgcaaagg tcatctctcg
      421 tttatttact tgttttagta aatgatggtg cttgcatata tatctggcga attaatcggt
      481 atagcagatg taatattcac agggatcact gtaattaaaa taaatgaagg attatgtaat
      541 ggaaaacttt aaacatctcc ctgaaccgtt ccgcattcgt gttattgagc cagtaaaacg
      601 taccactcgc gcttatcgtg aagaggcaat tattaaatcc ggtatgaacc cgttcctgct
      661 ggatagcgaa gatgttttta tcgatttact gaccgacagc ggcaccgggg cggtgacgca
      721 gagcatgcag gctgcgatga tgcgcggcga cgaagcctac agcggcagtc gtagctacta
      781 tgcgttagcc gagtcagtga aaaatatctt cggttatcaa tacaccattc cgactcacca
      841 gggccgtggc gcagagcaaa tctatattcc ggtactgatt aaaaaacgcg agcaggaaaa
      901 aggcctggat cgcagcaaaa tggtggcgtt ctctaactat ttctttgata ccacgcaggg
      961 ccatagccag atcaacggct gtaccgtgcg taacgtctat atcaaagaag ccttcgatac
     1021 gggcgtgcgt tacgacttta aaggcaactt tgaccttgag ggattagaac gcggtattga
     1081 agaagttggt ccgaataacg tgccgtatat cgttgcaacc atcaccagta actctgcagg
     1141 tggtcagccg gtttcactgg caaacttaaa agcgatgtac agcatcgcga agaaatacga
     1201 tattccggtg gtaatggact ccgcgcgctt tgctgaaaac gcctatttca ttaagcagcg
     1261 tgaagcagaa tacaaagact ggaccatcga gcagatcacc cgcgaaacct acaaatatgc
     1321 cgatatgctg gcgatgtccg ccaagaaaga tgcgatggtg ccgatgggcg gcctgctgtg
     1381 catgaaagac gacagcttct ttgatgtgta caccgagtgc agaacccttt gcgtggtgca
     1441 ggaaggcttc ccgacatatg gcggcctaga aggcggcgcg atggagcgtc tggcggtagg
     1501 tctgtatgac ggcatgaatc tcgactggct ggcttatcgt atcgcgcagg tacagtatct
     1561 ggtcgatggt ctggaagaga ttggcgttgt ctgccagcag gcgggcggtc acgcggcatt
     1621 cgttgatgcc ggtaaactgt tgccgcatat cccggcagac cagttcccgg caacaggcct
     1681 ggcctgcgag ctgtataaag tcgccggtat ccgtgcggta gaaattggct ctttcctgtt
     1741 aggccgcgat ccgaaaaccg gtaaacaact gccatgcccg gctgaactgc tgcgtttaac
     1801 cattccgcgc gcaacatata ctcaaacaca tatggacttc attattgaag cctttaaaca
     1861 tgtgaaagag aacgcggcga atattaaagg attaaccttt acgtacgaac cgaaagtatt
     1921 gcgtcacttc accgcaaaac ttaaagaagt ttaattaata ctacagagtg gctataagga
     1981 tgttagccac tctcttaccc tacatcctca ataacaaaaa tagccttcct ctaaaggtgg
     2041 catcatgact gttcaagctg aaaaaaagca ctctgcattt tgg
//