LOCUS       JX306131                 452 bp    DNA     linear   ENV 13-MAR-2013
DEFINITION  Uncultured ammonia-oxidizing bacterium clone SIP_XF6_12D ammonia
            monooxygenase (amoA) gene, partial cds.
ACCESSION   JX306131
VERSION     JX306131.1
KEYWORDS    ENV.
SOURCE      uncultured ammonia-oxidizing bacterium
  ORGANISM  uncultured ammonia-oxidizing bacterium
            Bacteria; environmental samples.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Peng,X., Yando,E., Hildebrand,E., Dwyer,C., Kearney,A., Waciega,A.,
            Valiela,I. and Bernhard,A.E.
  TITLE     Differential responses of ammonia-oxidizing archaea and bacteria to
            long-term fertilization in a New England salt marsh
  JOURNAL   Front Microbiol 3, 445 (2012)
   PUBMED   23346081
REFERENCE   2  (bases 1 to 452)
  AUTHORS   Peng,X., Yando,E., Hildenbrand,E., Dwyer,C., Kearney,A.,
            Waciega,A., Valiela,I. and Bernhard,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2012) Biology, Connecticut College, 270 Mohegan
            Avenue, New London, CT 06320, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="uncultured ammonia-oxidizing bacterium"
                     /mol_type="genomic DNA"
                     /isolation_source="salt marsh sediment"
                     /db_xref="taxon:933470"
                     /clone="SIP_XF6_12D"
                     /environmental_sample
                     /note="PCR_primers=fwd_name: amoA-1F, rev_name:
                     amoA-2R-TC"
     gene            <1..>452
                     /gene="amoA"
     CDS             <1..>452
                     /gene="amoA"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia monooxygenase"
                     /protein_id="AFU56356.1"
                     /translation="HYPINFVLPSTMIPGALIMDTTLLLTRNWMITALFGGGAFGLLF
                     YPGNWPIFGPTHLPLVVEGVLLSLADYTGFLYVRTGTPEYVRLIEQGSLRTFGGHTTV
                     IAAFFAAFVSMLMFVVWWYLGRFYCTSFYYVKGPRGRITEKHDVTAFG"
BASE COUNT           93 a          106 c          112 g          141 t
ORIGIN      
        1 cccattatcc gattaacttt gttctgccct ccacgatgat tcccggagcg ctgatcatgg
       61 acacaactct gttgctgacc cgtaactgga tgatcacagc attgttcggc ggcggagcct
      121 ttggactact attctatccg ggtaactggc cgatcttcgg accgactcac ctcccgttag
      181 tagtagaagg agtgctgctt tcgttggctg actataccgg attcctttat gttcgtactg
      241 gtacaccaga gtatgttcgg ttaattgaac aaggatcgtt acgtaccttc ggtggtcaca
      301 ctacggtgat tgctgcattc tttgcggctt ttgtatccat gttaatgttc gttgtatggt
      361 ggtacctggg aaggttctat tgcacctcgt tttattacgt caaaggtcct agaggacgga
      421 taacagagaa gcatgatgtt accgcttttg gc
//