LOCUS JO316361 901 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c55614_c mRNA sequence. ACCESSION JO316361 VERSION JO316361.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 901) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 901) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..901 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 202 a 190 c 274 g 229 t ORIGIN 1 cgtatggcgt gcccttgtca gtggcagcag cttctccgtc caagtgcagg cgattgcaca 61 ggtgcagtgt ctgaaacgca gggwmytcgg mmgggwggag gattgggaag atggcgtgaa 121 ggaggcggct cgatggcgcg gcaggtgtat gttggcaatc ggtggcgatg gcgggggatg 181 accatctgct gtctttctga tgtgcagaga gtgaacagaa gctgtggcag agggtctttg 241 gccgtggggt actcgccggg ttgtgtacat gggctgattg taataagtcg gaccgccaga 301 agctctcggg gtgctctgga gatggcggct caatgttggc gacgtcagct ccacgagaga 361 gagaggtgtg caccgcgcat ggcgcctgaa gaagagaaga tgacgaagcg ttcccctctc 421 gatttcccag ccgagtggga gaggccaggg cccgggcgaa ggcctgacat ttttccacaa 481 ttcactccaa tgaaaccacc cttgccgcag ccattgcctg gggatccccc agagaaagaa 541 gatgatgagg aggaggaggg cgacgatgac ccggaaccag aagaagaacc tgaaccagaa 601 gagtaattgg aaggaaggcg cattgctgtt ttcttcaatc atcttttcca agctttttgt 661 tcattggatt ctttggagac aaacctgtgt gatcaatttt catatcgaaa taactttttt 721 cttacctcat ttgcgtcaag agtgttgatg tgcatcgact ttatctattg ttagtgcgtg 781 cttctgtgat gaatgttatg tcactgtgat ccagggcctc tctttttgtt tgatgaaaac 841 cgtcaaacct tttgattctt tgcaaatatc agatttctca aaaggcgggg agttgtttct 901 a //