LOCUS       JO303347                 462 bp    mRNA    linear   TSA 23-FEB-2012
DEFINITION  TSA: Nitella hyalina strain KGK0190 nhya_c31621_c mRNA sequence.
ACCESSION   JO303347
VERSION     JO303347.1
DBLINK      BioProject: PRJNA51163
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Nitella hyalina
  ORGANISM  Nitella hyalina
            Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales;
            Characeae; Nitella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F.
  TITLE     Broad phylogenomic sampling and the sister lineage of land plants
  JOURNAL   PLoS ONE 7 (1), E29696 (2012)
   PUBMED   22253761
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  AUTHORS   Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2011) Cell Biology and Molecular Genetics,
            University of Maryland, Bioscience Research Building, College Park,
            MD 20947, USA
COMMENT     Two-step clustering process: First, trimmed 454 reads were
            clustered with MIRA v.2.9.43 using 454 EST assembly specific
            parameters. Second, the raw Sanger reads were combined with the 454
            contigs + singleons (with quality scores) and were cleaned then
            clustered with CAP3 (100 bp plus 95 percent identity overlap).
            
            ##Assembly-Data-START##
            Assembly Method       :: MIRA v. 2.9.43; CAP3
            Sequencing Technology :: 454 GS FLX Titanium; Sanger
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Nitella hyalina"
                     /mol_type="mRNA"
                     /strain="KGK0190"
                     /db_xref="taxon:181804"
BASE COUNT          122 a          114 c          105 g          121 t
ORIGIN      
        1 gaaaatgctt gccagacaga acttttccac tgtttccgag gaggccgtca atgcccaaat
       61 caacatggag ctcaaggctt cttacactta tgcctcgtta gctgcctact ttggtcgtga
      121 caacgttgcc cttcctggct ttgaaaagta cttcaagcat gcttccgagg aagagcgcga
      181 gcacgcccaa aaaatgatgg attacatcaa caagcgcgga ggtcgagttt ctttcgcccc
      241 tattgctgca cctgaagttg aatgggaatc cgccaaaaat gccgttgaat gcaccctcaa
      301 cctcgaacgt gaagtcaact tggccttgtt gaaactccgc agtgttgctg aagagcaaaa
      361 atgacgcaca tctttgcgac ttcattgaaa gcgaattcct tggtgagcaa gttgaaggta
      421 tcaaattcct tgctgatttg ttgaccaact ttgaaccgtg tt
//