LOCUS       JN129499                 960 bp    DNA     linear   BCT 09-NOV-2011
DEFINITION  Thermobifida fusca strain NRRL B-8184 cutinase (cut_1) gene,
            complete cds.
ACCESSION   JN129499
VERSION     JN129499.1
KEYWORDS    .
SOURCE      Thermobifida fusca
  ORGANISM  Thermobifida fusca
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Thermobifida.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Hegde,K.R. and Veeranki,V.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2011) Dept. of Biotechnology, Indian Institute of
            Technology Guwahati, Biochemical Engineering Laboratory, Guwahati,
            Assam 781039, India
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Thermobifida fusca"
                     /mol_type="genomic DNA"
                     /strain="NRRL B-8184"
                     /type_material="type strain of Thermomonospora fusca"
                     /db_xref="taxon:2021"
     gene            1..960
                     /gene="cut_1"
     CDS             1..960
                     /gene="cut_1"
                     /EC_number="3.1.1.74"
                     /note="cutin hydrolyzing protein"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase"
                     /protein_id="AET05798.1"
                     /translation="MPPHAARPGPAQNRRGRAMAVITPRRERSSLLSRALRFTAAAAT
                     ALVTAVSLAAPAHAANPYERGPNPTDALLEARSGPFSVSEERASRFGADGFGGGTIYY
                     PRENNTYGAVAISPGYTGTQASVAWLGERIASHGFVVITIDTNTTLDQPDSRARQLNA
                     ALDYMINDASSAVRSRIDSSRLAVMGHSMGGGGTLRLASQRPDLKAAIPLTPWHLNKN
                     WSSVRVPTLIIGADLDTIAPVLTHARPFYNSLPTSISKAYLELDGATHFAPNIPNKII
                     GKYSVAWLKRFVDNDTRYTQFLCPGPRDGLFGEVEEYRSTCPF"
BASE COUNT          163 a          398 c          259 g          140 t
ORIGIN      
        1 atgcccccgc atgcggcgcg gcccggccct gcacagaacc gaagaggacg tgcaatggct
       61 gtgattaccc cccgccggga gcgctcttcc ctgctctccc gggcactgcg cttcaccgcc
      121 gcggctgcca cagcgcttgt gaccgcggtc agcctggccg cccccgctca tgccgccaac
      181 ccctacgagc gcggccccaa cccgaccgac gccctgctcg aagcccgcag cggccccttc
      241 tccgtgagtg aagaacgggc ctcccgcttc ggtgctgacg gtttcggcgg cggcaccatc
      301 tactacccgc gggagaacaa cacctacggt gccgtggcga tctcccccgg ctacaccggc
      361 acccaggcct ctgtcgcctg gctgggcgag cgcatcgcct cccacggctt cgtcgtcatc
      421 accatcgaca ccaacaccac cctcgaccag ccggacagcc gggcccgcca gctcaacgcc
      481 gcgctggact acatgatcaa cgacgcctcg tccgcggtgc gcagccggat cgacagcagc
      541 cgactggcgg tcatgggcca ctccatgggc ggcggcggca ccctgcgtct ggcctcccag
      601 cgtcccgacc tgaaggccgc catcccgctc accccgtggc acctcaacaa gaactggagc
      661 agtgtgcggg ttcccaccct catcatcggt gctgacctgg acaccatcgc tccggtcctc
      721 acccacgccc ggcccttcta caacagcctc ccgacctcga tcagcaaggc ctacctggag
      781 ctggacggcg caacccactt cgccccgaac atccccaaca agatcatcgg caagtacagc
      841 gtcgcctggc tcaagcggtt cgtcgacaac gacacccgct acacccagtt cctctgcccc
      901 ggaccgcgcg acggactctt cggcgaggtc gaagagtacc gctccacctg ccccttctag
//