LOCUS       JH000482             1298986 bp    DNA     linear   CON 14-MAR-2015
DEFINITION  Cricetulus griseus cell line CHO-K1 unplaced genomic scaffold
            scaffold815, whole genome shotgun sequence.
ACCESSION   JH000482 AFTD01000000
VERSION     JH000482.1
DBLINK      BioProject: PRJNA69991
            BioSample: SAMN02981352
KEYWORDS    WGS.
SOURCE      Cricetulus griseus (Chinese hamster)
  ORGANISM  Cricetulus griseus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Cricetidae; Cricetinae; Cricetulus.
REFERENCE   1  (bases 1 to 1298986)
  AUTHORS   Xu,X., Nagarajan,H., Lewis,N.E., Pan,S., Cai,Z., Liu,X., Chen,W.,
            Xie,M., Wang,W., Hammond,S., Andersen,M.R., Neff,N., Passarelli,B.,
            Koh,W., Fan,C.H., Wang,J., Gui,Y., Lee,K.H., Betenbaugh,M.J.,
            Quake,S.R., Famili,I., Palsson,B.O. and Wang,J.
  TITLE     The genomic sequence of the Chinese hamster ovary CHO-K1 cell line
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1298986)
  AUTHORS   Xu,X., Nagarajan,H., Lewis,N.E., Pan,S., Cai,Z., Liu,X., Chen,W.,
            Xie,M., Wang,W., Hammond,S., Andersen,M.R., Neff,N., Passarelli,B.,
            Koh,W., Fan,C.H., Wang,J., Gui,Y., Lee,K.H., Betenbaugh,M.J.,
            Quake,S.R., Famili,I., Palsson,B.O. and Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2011) Beijing Genomics Institute in Shenzhen,
            Beishan Industrial Zone, Yantian District, Shenzhen, China
COMMENT     Source of DNA is available from ATCC Catalog No. CCL-61.
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: SOAPdenovo v. 1.05
            Assembly Name         :: CriGri_1.0
            Genome Coverage       :: 130x
            Sequencing Technology :: Illumina GA IIx
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298986
                     /organism="Cricetulus griseus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="scaffold815"
                     /db_xref="taxon:10029"
                     /chromosome="Unknown"
                     /cell_line="CHO-K1"
     assembly_gap    176..789
                     /estimated_length=614
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1194..1380
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1612..1621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    2241..2598
                     /estimated_length=358
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    9407..9431
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    22824..22932
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    23848..24069
                     /estimated_length=222
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    24438..25620
                     /estimated_length=1183
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    79545..79589
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    86372..86420
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    86761..86781
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    86995..87010
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    87330..87397
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    87765..87854
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    88129..90169
                     /estimated_length=2041
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    96132..96639
                     /estimated_length=508
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            97322..135062
                     /locus_tag="I79_011484"
     mRNA            join(97322..97408,98352..98419,100153..100224,
                     100989..101057,103340..103442,106822..106914,
                     118625..118692,119648..119726,122095..122177,
                     134954..135062)
                     /locus_tag="I79_011484"
                     /product="UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose
                     transporter"
     CDS             join(97322..97408,98352..98419,100153..100224,
                     100989..101057,103340..103442,106822..106914,
                     118625..118692,119648..119726,122095..122177,
                     134954..135062)
                     /locus_tag="I79_011484"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose
                     transporter"
                     /protein_id="EGV99054.1"
                     /db_xref="InterPro:IPR004853"
                     /db_xref="UniProtKB/Swiss-Prot:Q5RDC9"
                     /db_xref="UniProtKB/TrEMBL:Q3TE90"
                     /translation="MVATVAVLWVGKALRVVKFPDFDRNVPRKTFPLPLLYFGNQITG
                     LFSTKKLNLPMFTVLRRFSILFTMFAEGALLKKTFSWGIKMTVFAMIIGAFVAASSDL
                     AFDLEGYVFILINDVLTAANGAYVKQKLDSKELGKYGLLYYNALFMILPTMAIAYFTG
                     DAQKAMEFEGWTDTLFLLQFTLSCVMGFILMYATVLCTQYNSALTTTIVGCIKNILIT
                     YIGMVFGGDYIFTWTNFIGLNISIAGSLVYSYITFTEEQLSKQSEANNKLDTKGKGAV
                     "
     assembly_gap    113611..113655
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            complement(150522..214895)
                     /locus_tag="I79_011485"
     mRNA            complement(join(150522..150850,151398..151446,
                     167621..167715,170123..170204,171054..171205,
                     182190..182262,184637..184701,185583..185715,
                     186320..186478,196609..196754,200997..201021,
                     214839..214895))
                     /locus_tag="I79_011485"
                     /product="Mesoderm induction early response protein 1"
     CDS             complement(join(150522..150850,151398..151446,
                     167621..167715,170123..170204,171054..171205,
                     182190..182262,184637..184701,185583..185715,
                     186320..186478,196609..196754,200997..201021,
                     214839..214895))
                     /locus_tag="I79_011485"
                     /codon_start=1
                     /product="Mesoderm induction early response protein 1"
                     /protein_id="EGV99055.1"
                     /db_xref="InterPro:IPR000949"
                     /db_xref="UniProtKB/Swiss-Prot:Q5REE1"
                     /db_xref="UniProtKB/TrEMBL:Q32P49"
                     /translation="MKTGEWVMFQELIEAFQNSPSVESSSPGGSATSEDHEFDPSADM
                     LVHDFDDERTLEEEEMMEGETNFSSEIEDLAREGDMPIHELLSLYGYDSTRLPEEDEE
                     EEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQEDETQSSNDDPSQSVTSQDAQ
                     EIIRPRRCKYFDTNSEIEEESEEDEDYIPSEDWKKEIMVGSMFQAEIPVGVCRYKENE
                     KVYENDDQLLWDPEYLPEDKVIVFLKDASRRTGDERGVEAIPEGSHIKDNEQALYELV
                     KCSFDTEEALRRLRFNVKAAREELSVWTEEECRNFEQGLKAYGKDFHLIQANKGSIRP
                     LSDPTSYGNNVSVSSNGPGEILNKEEVKVEGLHVNGPTGGNKKPLHTDMDTNGYEADN
                     VTTDPKLAHMTARNENDFDEKNERPAKRRRINSNGKESPGSSEFFQEAVSHGKFEEHE
                     NIND"
     assembly_gap    152453..153729
                     /estimated_length=1277
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    155245..161316
                     /estimated_length=6072
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    161498..163229
                     /estimated_length=1732
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    176896..177425
                     /estimated_length=530
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    177769..180150
                     /estimated_length=2382
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    208636..209074
                     /estimated_length=439
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    213589..214095
                     /estimated_length=507
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            226869..289892
                     /locus_tag="I79_011486"
     mRNA            join(226869..226934,233356..233540,235039..235151,
                     241685..241841,244952..245091,246774..246920,
                     253727..253921,262391..262614,265278..265364,
                     267093..267244,269656..269813,270102..270223,
                     277053..277151,277594..277820,289841..289892)
                     /locus_tag="I79_011486"
                     /product="WD repeat-containing protein 78"
     CDS             join(226869..226934,233356..233540,235039..235151,
                     241685..241841,244952..245091,246774..246920,
                     253727..253921,262391..262614,265278..265364,
                     267093..267244,269656..269813,270102..270223,
                     277053..277151,277594..277820,289841..289892)
                     /locus_tag="I79_011486"
                     /codon_start=1
                     /product="WD repeat-containing protein 78"
                     /protein_id="EGV99056.1"
                     /db_xref="InterPro:IPR001680"
                     /db_xref="InterPro:IPR011046"
                     /db_xref="InterPro:IPR017986"
                     /db_xref="InterPro:IPR019781"
                     /db_xref="InterPro:IPR019782"
                     /db_xref="UniProtKB/Swiss-Prot:Q4V8G4"
                     /db_xref="UniProtKB/TrEMBL:A0AVI9"
                     /translation="MLSVPEMKPAEKLSITVTKTIQVFDAAGVDVTPRPLYHPDPLVG
                     TAKPNKLLTSQEGSLTSDFISSYSLYQNTINPSMLGQFTRSVLGSSTVSKSSVSTTES
                     MTEDLEEPAYKRERLASFTDVRVLRPTPETTITKEELQKNIQILLTETETLKFFDLPT
                     VIFSVESEEAEKVIEKNKNYEILCRNRLGNDLYVERMMQTFNGAPKNKEVQCEKIIME
                     EKGIMATAWDLFDSFNTPEALLSAKQSLVGSKGNLLAKDRDLKLQGKYHGQNSETSSL
                     MDIENVILAKVHEDDEGNSEAILKSEKLQQDLFFMERVLMENVFQSKLAAYRQLPVLK
                     EYEPEEPEEILEEESIEESDEESKEEEEETEVELELEIATEQSTIPANLERLWSFSCD
                     LTKGLNVSSLSWNKANPDLLAVGYGNFGFKEQKRGMACCWSIKNPMWPERIYQSAYGV
                     TSVDFSIATPNLLAVGYHNGTIAIYNVRSNNNFTVLDSSESPQKHLGPVWQLQWIEQD
                     RGTTGDDKREILVSISSDGRISKWIIRKGLDCHDLMRLRRTTAGSNKKGPEKEKKGEA
                     LISRQAPGMCFAFHPKDTNVYLVGTEEGLIHKCSCSYNEQYLDTYRGHKGPVYKVTWN
                     PFCQDVFLSCSADWGVMVWRQETFKPVLSFYPTTYVVYDVAWSPKSAYIFAAANESRV
                     EIWDLQISTRLPAVLVTSLTYPGNI"
     assembly_gap    239972..240637
                     /estimated_length=666
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    242123..243389
                     /estimated_length=1267
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            297299..300395
                     /locus_tag="I79_011487"
     mRNA            join(297299..297473,300163..300395)
                     /locus_tag="I79_011487"
                     /product="Insulin-like peptide INSL5"
     CDS             join(297299..297473,300163..300395)
                     /locus_tag="I79_011487"
                     /note="GO_component: GO:0005576 - extracellular region
                     [Evidence IEA];
                     GO_function: GO:0005179 - hormone activity [Evidence IEA]"
                     /codon_start=1
                     /product="Insulin-like peptide INSL5"
                     /protein_id="EGV99057.1"
                     /db_xref="InterPro:IPR004825"
                     /db_xref="InterPro:IPR016179"
                     /db_xref="UniProtKB/Swiss-Prot:Q9WUG6"
                     /db_xref="UniProtKB/TrEMBL:A2AIY3"
                     /translation="MKGPTLALFLFSVLLAVVQVRGRETVKLCGLEYVRTVIYICASS
                     RWRRSLKEAPQPQQAEPRDYFQLPDRQETSKETLEHILPKMDTSGQELVQDPQAPMEK
                     LWQSKKYSVVSKRDLQALCCTEGCSMTELSTLC"
     assembly_gap    322249..322522
                     /estimated_length=274
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            complement(346643..357635)
                     /locus_tag="I79_011488"
     mRNA            complement(join(346643..346665,348170..348334,
                     348869..348933,349412..349614,350483..350615,
                     352638..352793,357553..357635))
                     /locus_tag="I79_011488"
                     /product="SH3-containing GRB2-like protein 3-interacting
                     protein 1"
     CDS             complement(join(346643..346665,348170..348334,
                     348869..348933,349412..349614,350483..350615,
                     352638..352793,357553..357635))
                     /locus_tag="I79_011488"
                     /codon_start=1
                     /product="SH3-containing GRB2-like protein 3-interacting
                     protein 1"
                     /protein_id="EGV99058.1"
                     /db_xref="InterPro:IPR018808"
                     /db_xref="UniProtKB/Swiss-Prot:Q8VD37"
                     /db_xref="UniProtKB/TrEMBL:A2AIZ0"
                     /translation="MGAQDTLPVAAAFTETVNAYFKGADPSKCIVKITGEMVLSFPAG
                     ITRHFANNPSPAALTFRVINSSRLEHVLPNPQLLCCDNTQNDANTKEFWVNMPNLMTH
                     LKKVSEQKPQATYYNVDMLKYQVSAQGIQSTPLNLAVNWRCEPASTDLRIDYKYNTDA
                     MTTAVALNNVQFLVPIDGGVTKLQAVLPPAVWNAEQQRILWKIPDISQKSENGGVGSL
                     LARFQLSDGPSKPSPLVVQFTSEGSTLSGCDIELVGAGYRFSLIKKRFAAGKYLADN"
     assembly_gap    494906..494932
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    528138..528250
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    586517..586529
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    624803..624826
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    635803..635896
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            663561..663902
                     /locus_tag="I79_011489"
     mRNA            663561..663902
                     /locus_tag="I79_011489"
                     /product="hypothetical protein"
     CDS             663561..663902
                     /locus_tag="I79_011489"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="EGV99059.1"
                     /translation="MGINYIPVLPAPIKRYKINLSINHLVSNMRILFSKPAHQRVGQN
                     EGLRIRKGRPLEQRPFLSSLLLGLHVSFKQHLDYIIHNTKHYKKRLQLLGRVFYTNVT
                     PPLAGNVRYIN"
     gene            complement(664920..699856)
                     /locus_tag="I79_011490"
     mRNA            complement(join(664920..665285,667584..667766,
                     668449..668571,668662..668816,670617..670716,
                     672522..672686,672824..672922,674153..674331,
                     679859..679952,699767..699856))
                     /locus_tag="I79_011490"
                     /product="cAMP-specific 3',5'-cyclic phosphodiesterase 4B"
     CDS             complement(join(664920..665285,667584..667766,
                     668449..668571,668662..668816,670617..670716,
                     672522..672686,672824..672922,674153..674331,
                     679859..679952,699767..699856))
                     /locus_tag="I79_011490"
                     /note="GO_function: GO:0003824 - catalytic activity
                     [Evidence IEA];
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /product="cAMP-specific 3',5'-cyclic phosphodiesterase 4B"
                     /protein_id="EGV99060.1"
                     /db_xref="InterPro:IPR002073"
                     /db_xref="InterPro:IPR003607"
                     /db_xref="UniProtKB/Swiss-Prot:P14646"
                     /db_xref="UniProtKB/TrEMBL:Q8VBU5"
                     /translation="METLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSE
                     MSRSGNQVSEYISNTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLN
                     NTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYAIFQERDLLK
                     TFKISSDTFVTYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLEIL
                     AAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIF
                     QNLTKKQRQTLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRI
                     QVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASV
                     EKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPPLDERSR
                     DCQGLMEKFQFELTLEEEDSEGPEKEGEGHNYFSSTKTLCVIDPESRDSLEDTDIDIA
                     TEDKSPIDT"
     assembly_gap    667495..667510
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    706288..706323
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    889709..889718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    890375..890436
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1018777..1019060
                     /estimated_length=284
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1061881..1061947
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1093897..1093906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1227580..1227874
                     /estimated_length=295
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1228191..1228831
                     /estimated_length=641
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1240038..1240084
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1242313..1242322
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1260910..1260941
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1283587..1287901
                     /estimated_length=4315
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1289847..1289856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1290134..1290213
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     assembly_gap    1294312..1294778
                     /estimated_length=467
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
CONTIG      join(AFTD01045848.1:1..175,gap(614),AFTD01045849.1:1..404,gap(187),
            AFTD01045850.1:1..231,gap(10),AFTD01045851.1:1..619,gap(358),
            AFTD01045852.1:1..6808,gap(25),AFTD01045853.1:1..13392,gap(109),
            AFTD01045854.1:1..915,gap(222),AFTD01045855.1:1..368,gap(1183),
            AFTD01045856.1:1..53924,gap(45),AFTD01045857.1:1..6782,gap(49),
            AFTD01045858.1:1..340,gap(21),AFTD01045859.1:1..213,gap(16),
            AFTD01045860.1:1..319,gap(68),AFTD01045861.1:1..367,gap(90),
            AFTD01045862.1:1..274,gap(2041),AFTD01045863.1:1..5962,gap(508),
            AFTD01045864.1:1..16971,gap(45),AFTD01045865.1:1..38797,gap(1277),
            AFTD01045866.1:1..1515,gap(6072),AFTD01045867.1:1..181,gap(1732),
            AFTD01045868.1:1..13666,gap(530),AFTD01045869.1:1..343,gap(2382),
            AFTD01045870.1:1..28485,gap(439),AFTD01045871.1:1..4514,gap(507),
            AFTD01045872.1:1..25876,gap(666),AFTD01045873.1:1..1485,gap(1267),
            AFTD01045874.1:1..78859,gap(274),AFTD01045875.1:1..172383,gap(27),
            AFTD01045876.1:1..33205,gap(113),AFTD01045877.1:1..58266,gap(13),
            AFTD01045878.1:1..38273,gap(24),AFTD01045879.1:1..10976,gap(94),
            AFTD01045880.1:1..31598,gap(16),AFTD01045881.1:1..38777,gap(36),
            AFTD01045882.1:1..183385,gap(10),AFTD01045883.1:1..656,gap(62),
            AFTD01045884.1:1..128340,gap(284),AFTD01045885.1:1..42820,gap(67),
            AFTD01045886.1:1..31949,gap(10),AFTD01045887.1:1..133673,gap(295),
            AFTD01045888.1:1..316,gap(641),AFTD01045889.1:1..11206,gap(47),
            AFTD01045890.1:1..2228,gap(10),AFTD01045891.1:1..18587,gap(32),
            AFTD01045892.1:1..22645,gap(4315),AFTD01045893.1:1..1945,gap(10),
            AFTD01045894.1:1..277,gap(80),AFTD01045895.1:1..4098,gap(467),
            AFTD01045896.1:1..4208)
//