LOCUS JAGRCU010000163 7050 bp DNA linear ENV 12-OCT-2021 DEFINITION MAG: Caldilineaceae bacterium isolate HKST-UBA34 NODE_164_length_7050_cov_0.955113, whole genome shotgun sequence. ACCESSION JAGRCU010000163 JAGRCU010000000 VERSION JAGRCU010000163.1 DBLINK BioProject: PRJNA432264 BioSample: SAMN14563025 KEYWORDS WGS; Metagenome Assembled Genome; MAG. SOURCE Caldilineaceae bacterium (activated sludge metagenome) ORGANISM Caldilineaceae bacterium Bacteria; Chloroflexi; Caldilineae; Caldilineales; Caldilineaceae. REFERENCE 1 (bases 1 to 7050) AUTHORS Wang,Y., Ye,J., Ju,F., Liu,L., Boyd,J.A., Deng,Y., Parks,D.H., Jiang,X., Yin,X., Woodcroft,B.J., Tyson,G.W., Hugenholtz,P., Polz,M.F. and Zhang,T. TITLE Successional dynamics and alternative stable states in a saline activated sludge microbial community over 9 years JOURNAL Microbiome 9 (1), 199 (2021) PUBMED 34615557 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 7050) AUTHORS Zhang,T. TITLE Direct Submission JOURNAL Submitted (13-APR-2021) Civil Engineering, The University Hong Kong, Pokfulam Road, Hong Kong 999077, Hong Kong COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: CLC de novo assembler v. 6.04 Genome Representation :: Full Expected Final Version :: No Genome Coverage :: 134x Sequencing Technology :: Illumina HiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 04/20/2021 04:49:41 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 5.1 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 6,029 CDSs (total) :: 5,991 Genes (coding) :: 5,969 CDSs (with protein) :: 5,969 Genes (RNA) :: 38 rRNAs :: 1 (16S) partial rRNAs :: 1 (16S) tRNAs :: 35 ncRNAs :: 2 Pseudo Genes (total) :: 22 CDSs (without protein) :: 22 Pseudo Genes (ambiguous residues) :: 0 of 22 Pseudo Genes (frameshifted) :: 2 of 22 Pseudo Genes (incomplete) :: 20 of 22 Pseudo Genes (internal stop) :: 0 of 22 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..7050 /organism="Caldilineaceae bacterium" /mol_type="genomic DNA" /submitter_seqid="NODE_164_length_7050_cov_0.955113" /isolate="HKST-UBA34" /isolation_source="activated sludge from Shatin waste water treatment plant collected monthly from 2007 through 2015" /db_xref="taxon:2614250" /environmental_sample /geo_loc_name="China:Hong Kong SAR, Shatin waste water treatment plant" /lat_lon="22.406236 N 114.213394 E" /metagenome_source="activated sludge metagenome" /note="metagenomic" gene <1..780 /locus_tag="KDE20_07760" CDS <1..780 /locus_tag="KDE20_07760" /inference="COORDINATES: protein motif:HMM:NF015679.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="L,D-transpeptidase" /protein_id="MCB0071337.1" /translation="LVEVSGHADHPTFRKWQLDLLLNGDPAQATFLAVGEDPVHTPMT LATIDTRRYPDGNHSLRLRVVHSNLNYDEYIVPITVKNSASGDAGRPAAIASLTDAEE DKLLAVGEANVPAAKPAVKPVAKPGVPAPKANNLTFTPPPGGRWIEVDISDQSLTAWD GDRIFMQTQISSGRAETPTVAGVFSVERKLERQRMVGPGYDLPNVTSVMYFFLNYAIH AAYWHDDFGSVASHGCVNMRVDEAATLFDWAEVGTPVYVHD" gene 1063..2535 /locus_tag="KDE20_07765" CDS 1063..2535 /locus_tag="KDE20_07765" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MCB0071338.1" /translation="MKRTLHFLPALIIALAAWFGFARPAAAACTGSDEPTPNLRAYDV ACAAGVCTLDVPLELAPLPKLGAQIAIDKLENNLTFLPDGTTLDLTDSLSVKLPVGEI LLRNADLNMTLGEDGAVEQLRGTAEVPLPRLGILENVALTGPVKADVGFERGANLADL NAPLDPDRQYMFIHFGSGLDLTADHVAADGETRTLAINIPQGQRATLIIDTEEPFAYL AGNLTLRYDEQLAFVGEWLSAADLASGIPLRHSVGVEFTGAMGENQPLFLRVGGSYAA DAGLVGRWVGMDITPLAVHGVMTINDQGMLLDGVAHTEIFPETFMDGTMATQVFIPFE GELRDAYVKVDAQAEFPLAHATVEGTAMVNGALDVQARAGVTTPMYDNDGLVLVESDG DAATQRLARARDWAGGATDTVTRSFVYVRDGTGRGLDHVVDGVTWSAVAMGDGAAAGW DWTQARWCGVTGICGDGAATQADAIAQADATAEVALNAID" gene 2858..3139 /locus_tag="KDE20_07770" CDS 2858..3139 /locus_tag="KDE20_07770" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014431871.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="Lrp/AsnC ligand binding domain-containing protein" /protein_id="MCB0071339.1" /translation="MVTAIVLINVKHGHVNDVAERLVEMKGVAEVYSVGGRYDLVAII RVKNNDQMAELVTNDMLALDNIDNTETLIAFKAFSEHDLERMFAIGIDD" gene 3149..3712 /locus_tag="KDE20_07775" CDS 3149..3712 /locus_tag="KDE20_07775" /inference="COORDINATES: protein motif:HMM:NF018024.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DUF1028 domain-containing protein" /protein_id="MCB0071340.1" /translation="MMNTSQPIATFSIVAYDPGRVEWGVAVQSKFLAVGAVVPWARAG AGVVATQSYANLAYGPDGLDLMAQGMSAAETLTTLTAADPGRAQRQAGLVDQAGRAAA FTGDECHFWAGHHVGDGYACQGNILVPGTVEAMAAAFEDAARAGRTGRLAGDGVGRGT GRGRQQPGPPGCGRDGGARRRRLRRQQ" gene 3615..4031 /locus_tag="KDE20_07780" CDS 3615..4031 /locus_tag="KDE20_07780" /inference="COORDINATES: protein motif:HMM:NF018024.1,HMM:NF020402.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DUF1028 domain-containing protein" /protein_id="MCB0071341.1" /translation="MAAGQAAGGNSRGRQAAAVTVVRAGGGYGGNNDRYLDLRVDDDP TPIARLQQLVAMHHLYFGAVDPANVVPLAGIAAELQAMLRATGDYTGPQNGEFDAATR TALRALVGRENLEERWDGTADAIDGLALDYLRRRFG" gene 4041..5024 /locus_tag="KDE20_07785" CDS 4041..5024 /locus_tag="KDE20_07785" /inference="COORDINATES: protein motif:HMM:NF013530.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="NAD(P)-dependent oxidoreductase" /protein_id="MCB0071342.1" /translation="MGNTAQPAGAERVLVTGALGCIGAWVTRTLVRDGAQVIAFDLGI NRSRLELVMPPDELAAVHFERGDITDRAAVVDCVSVQGITHIIHLAALQVPFCKADPV AGARVNVVGTVNVFEAAKAAGLSQVVYASSIAVYGPAGHYADAILPADAPLFPQTHYG VYKQANEGTARIFWQDDGIASVALRPYTIYGPARDQGLTSEPTKAMLAAARGDGYHIP FGGVMGYQHVEDVARIFVQAMRTPVEGAHAFNLRGAIADMPTVIAAIEAAAPDVAGRI SYDEVALPFPQGFDDQALRAALGAIPDTSLVDGVAKTVDHFRIALADGRLS" gene 5125..5934 /locus_tag="KDE20_07790" CDS 5125..5934 /locus_tag="KDE20_07790" /inference="COORDINATES: protein motif:HMM:NF013706.1,HMM:NF021856.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="fumarylacetoacetate hydrolase family protein" /protein_id="MCB0071343.1" /translation="MKWVRVADDGGARYGVLDGETIRLTSDTWDDLLNGVTPTYLDDV AAESATLLNPVPAPGKIVAIGLNYLDHCRETGMEVPKSPLVFTKFNTSLVGPGDEIVW SASLTQKVDYEAELAVVMGKTARNVDVDDALAYVFGYTAANDVSARDIQLGDGQWVRG KSLDTFCPVGPAVVTADEIPDPGALAIRSILNGKVMQDSNTSELIFGVAELIAFCSRS FTLEPGDLILTGTPFGVALGRQPEVYMQDGDEIVVEIEGIGRLVNTCRVVA" gene 6069..7010 /locus_tag="KDE20_07795" CDS 6069..7010 /locus_tag="KDE20_07795" /inference="COORDINATES: protein motif:HMM:NF019500.1,HMM:NF036967.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MCB0071344.1" /translation="MQKTDDRYAQYRGGRFNYRLTGISQAPLSDIYYLLISKPWPWLL ATAFVGYLVINGGFALLYWLGGDGIQGAQPGSFLDAFYFSVQTFSTVGYGVLSPKTPY AHALATAESFAGMLAVAVGTGIIFAKFSRPSARVTFSEVMVVHPRNGVPTLQFRVANQ RRNEIFAAHMNVHVLMEETTAEGHRMRRMHKLVLEREEMPVFTVAWNALHPLDGASPL YGMNANVCNQNLVFIVVAFSGTDGTFLQTVQARHLYMSKDVRFDSQFVDMIENGKDGG MVVHHELLHATQPFEQNGSTMALPVAAEKSAAETPQQ" BASE COUNT 1261 a 2340 c 2301 g 1148 t ORIGIN 1 ctggtcgagg tcagcggcca cgccgatcat cccacgtttc gcaaatggca actggatctg 61 cttctgaacg gcgacccggc acaggctacg tttctggcag tgggtgaaga ccccgtacac 121 acgccgatga cgctggccac aattgacacg cggcgctatc ccgacggcaa tcacagcctg 181 cgcctgcgcg tggtccacag caatctgaac tacgacgagt acattgtccc cattacggtg 241 aagaacagtg cgagcggcga tgccggacgt ccggccgcga tcgcctcgct caccgatgcg 301 gaggaggaca agctgctcgc cgtgggcgag gcgaatgtgc cggccgccaa gccggccgtc 361 aaaccggtcg ccaaaccggg cgtgcccgcg cccaaggcca acaatctcac cttcacgccc 421 ccgcccggcg gccgctggat tgaggtcgac atttcggacc agagcctgac cgcgtgggac 481 ggcgaccgca tcttcatgca gacgcagatc agttcgggtc gcgccgagac gccgaccgtg 541 gcaggcgtct tcagcgtgga acgcaagctc gaacgccaac gcatggtcgg tcccggctac 601 gatctcccta acgtgacgtc ggtgatgtac ttcttcctca actacgccat ccacgccgca 661 tactggcacg acgacttcgg cagcgtggcc agccacggct gcgtcaacat gcgcgtggac 721 gaagccgcca cgctcttcga ctgggccgag gtcggcacgc cggtctacgt ccacgattga 781 cgcgtggtgc cttcgcgcct tttctccacc aattttccat cccgcggaac caattgacgc 841 cgcgctgcgt catctcgatg gccggccggt gtgcagagat gcaaatgcaa cgctaacgcg 901 gtgcatagac ggaactcaca cgggtttgcc atactgagaa gcggcaattg cccaccgtag 961 cttatggcgc gctgcgtcgt atggtctgtt gcaggctacg gtccatcaca accggacccg 1021 tccatgcagg cgggccctgc accaaccgtg ggaggagcgt ccatgaaacg aacactgcac 1081 tttttgccgg cgctaatcat tgcgctggcg gcctggttcg gctttgcccg gccggccgcc 1141 gctgcctgca ccggcagcga tgaaccgaca cccaacctgc gcgcctacga tgtggcgtgc 1201 gccgccggcg tctgcacgct cgacgtgccg ctggagctgg cgccgctgcc caagctgggt 1261 gcgcagatcg cgatcgataa gctggaaaac aacctgacct tcctccccga cggcacgacc 1321 ctggacctga ccgacagcct cagcgtcaag ctgcccgtgg gcgaaatcct gctgcgcaac 1381 gccgacctca acatgacctt gggcgaagac ggcgccgtgg agcagttgcg cggcaccgcg 1441 gaagtgccgc tgccgcgcct gggcatcctg gagaacgtgg cgttgaccgg gcccgtcaag 1501 gcggacgtcg gcttcgagcg gggcgccaat ctggctgacc tcaacgcacc gctggacccg 1561 gatcggcagt acatgttcat ccacttcggc tcgggcctgg acctgacggc ggaccacgtg 1621 gccgcggacg gtgagacgcg gacgttggcc atcaacattc cccagggcca gcgcgccacg 1681 ctcatcatcg acaccgagga acccttcgcc tatctggcgg gcaacctcac cctgcgctac 1741 gacgagcaac tggcgttcgt gggcgagtgg ctgagcgcag ccgacctggc cagtggcatt 1801 ccgctgcgcc acagcgtggg cgtggaattc accggcgcca tgggcgaaaa ccagcccctc 1861 ttcctgcgcg tcggcggcag ctatgcggcc gacgccgggc tggtgggccg ctgggtgggc 1921 atggatatca ccccgctcgc cgtacacggc gtcatgacca tcaacgacca gggcatgttg 1981 ttggacggtg tggcccacac cgagatcttt ccggaaacct tcatggacgg caccatggcg 2041 acccaggtgt tcatcccctt cgagggcgag ctgcgtgacg cctatgtgaa ggtcgatgcg 2101 caggccgaat tccccctggc ccacgccact gtggaaggca cggccatggt caacggtgcg 2161 ctggacgtgc aggcgcgcgc cggcgtcacc acgcccatgt acgacaatga cggcctggtg 2221 ctggtggaaa gcgacggcga cgccgcgacc caacggctgg cccgggcgcg tgattgggcc 2281 ggcggcgcga ccgacacggt cacccgcagc ttcgtctacg tgcgcgacgg cacgggccgc 2341 ggcctggatc acgtcgtgga cggcgtgacc tggagcgccg tggccatggg tgacggcgcg 2401 gccgcaggct gggactggac gcaggcccgc tggtgcggcg tcaccggtat ctgcggcgat 2461 ggcgcggcta cgcaagctga cgccattgca caggcggacg ccaccgcgga agtggccctg 2521 aacgccatcg attgacatgt ctcccgccgg tgatcggctg cgcaggccct gaacgcgtca 2581 atggcgacgc cgtggggccg cagccggtca ccgccactca aatacaccgt ctgtcaccat 2641 cccattgcac gagggagtac aagcccgggt cacgtcacga cccgggcttt tgcattgggg 2701 ggctcgttct gtatacccca aattgagatg cgccccatcc gagcagtgag attgcacgcc 2761 gtccggtccg gtggtaacat cgacctcagt cgcacgcaaa gctcaaatcc acggcgggct 2821 gccgccggac gaaccacagc atcaaggaga atcctgcatg gtcaccgcaa ttgtgctgat 2881 caacgtgaag catggccacg tcaacgacgt cgccgagcgc ctggtggaga tgaagggcgt 2941 ggccgaggtc tactcggtgg gcgggcgcta cgacctggtc gccatcatcc gcgtcaagaa 3001 caacgaccag atggccgagt tggtcaccaa cgacatgctc gcgctggaca acatcgacaa 3061 caccgagacc ctcatcgcgt tcaaggcgtt ctccgagcac gacctggagc gcatgttcgc 3121 catcggcatc gatgactaag acgcccccat gatgaacacc agccagccaa ttgccacctt 3181 ctccatcgtc gcctacgatc ctggacgcgt ggaatggggc gtggccgtgc agtccaagtt 3241 cctggccgtg ggcgccgtgg tgccgtgggc ccgggccggc gccggcgtgg tcgccaccca 3301 gtcctacgcc aacctcgcct acggtcccga cggactcgac ctcatggccc agggcatgtc 3361 cgccgcagag acgctgacga ccctgaccgc cgccgatccc ggacgcgccc agcgtcaggc 3421 cggtctcgtc gatcaggcgg gacgcgccgc ggcctttacc ggcgacgagt gccacttctg 3481 ggccgggcac cacgtgggcg acgggtatgc gtgccagggc aatattctcg tgccgggcac 3541 ggtggaggcc atggccgccg cgtttgaaga cgcggcgcgg gccgggcgaa ctggccgact 3601 ggctggtgac ggcgttggcc gcgggacagg ccgcgggcgg caacagccgg ggccgccagg 3661 ctgcggccgt gacggtggtg cgcgccggcg gcggctacgg cggcaacaat gaccgctacc 3721 tggacctgcg cgtcgacgac gatcctacgc ccatcgcgcg cctgcaacag ttggtcgcca 3781 tgcatcacct ctacttcggc gcggtggatc ccgccaacgt ggtgcccctg gccgggatcg 3841 cggctgaact ccaggccatg ctgcgcgcta ccggcgacta caccggtccc cagaacggcg 3901 agtttgacgc ggccacacgc accgccctgc gcgcgctcgt cggccgcgaa aatttggaag 3961 agcggtggga cggaaccgcc gatgcgatcg atgggctggc gctggactac ctgcgccgcc 4021 ggttcggata ggagagactc atgggaaaca ctgcacagcc ggccggtgcg gaacgcgtgc 4081 tcgtcaccgg cgcgttgggc tgcatcggcg cgtgggtcac gcgcacgctg gtgcgggacg 4141 gtgcgcaggt gatcgccttt gacctgggca tcaatcgcag ccgtctggaa ctggtcatgc 4201 cgccggacga actcgccgcc gtccacttcg agcgcggcga catcaccgac cgcgccgcgg 4261 tggtggactg cgtgagcgta cagggcatca cccacatcat ccacctggcg gcgctgcaag 4321 tgcccttctg caaggccgac ccggtggccg gtgcgcgcgt caacgtggtc gggacggtca 4381 acgtcttcga agcggccaag gcagccgggc tgagccaggt cgtctacgcc agcagcatcg 4441 cggtctacgg gccggccggc cactatgccg atgcgatttt accggccgat gcgccgctct 4501 ttccccaaac ccactacggc gtctacaaac aggccaacga gggcaccgcc cgcattttct 4561 ggcaggacga cggcatcgcc agcgtggccc tgcgtccgta caccatttac ggcccggcgc 4621 gcgaccaggg cctcacctcc gagccgacca aggccatgtt ggccgcggcc cggggcgacg 4681 gctatcacat tcccttcggc ggcgtcatgg gctatcagca cgtggaggac gtggcgcgca 4741 tcttcgtgca ggccatgcgc acgcccgtgg agggcgcgca cgccttcaac ctgcgcggcg 4801 cgatcgcgga catgcccacg gtcatcgcgg ccatcgaagc cgccgcgccg gatgtggccg 4861 gccgcatcag ctacgacgag gtggcgctgc ccttccccca aggctttgac gaccaggcgt 4921 tgcgcgccgc gctgggcgcc attcccgata catcactggt cgacggcgtg gccaagaccg 4981 tggaccattt ccgcatcgcg ctggccgacg ggcgcctgtc ctgacgacgg cgggaaacga 5041 gtgcggcaga gagatcacaa acgaccggcg cgcagaacaa agtgcgcgcc gcgaaaccat 5101 ccagagacga agggaagaga ccagatgaaa tgggttcgcg ttgccgatga cggcggagca 5161 cgctacggcg ttttggacgg ggagaccatc cggctgacga gcgacacgtg ggacgatctg 5221 ctcaacggcg tcacaccgac ctacttggac gacgtcgccg cggagagcgc caccctgctc 5281 aatcccgtgc ccgcgccggg caagatcgtg gccatcgggc tgaactacct ggatcactgc 5341 cgtgagacgg ggatggaggt gccgaaatcg ccgctggttt tcaccaagtt caacacgtcg 5401 ctggtgggtc cgggcgacga gattgtctgg agcgcgagcc tgacccagaa ggtcgactac 5461 gaggcagaac tggccgtggt catgggcaag acggcgcgca acgtggacgt ggacgacgcg 5521 ctggcgtacg tcttcggcta cactgcggcc aatgacgtga gcgcgcgcga catccagttg 5581 ggcgacggcc agtgggtgcg gggcaagagt ctggacacct tctgccccgt gggtccggcc 5641 gtggtcaccg ccgacgagat tcccgacccc ggcgcgctgg ccatccgcag catcctcaac 5701 ggcaaagtca tgcaggacag caacacgtcc gagttgatct tcggcgtggc cgagctgatt 5761 gcgttctgtt cccgctcctt caccctggaa ccgggcgacc tgatcctcac cggcacaccc 5821 ttcggcgtgg ccctgggccg gcagccggag gtctacatgc aggatggcga cgagatcgtg 5881 gtcgagattg aggggatcgg gcggctggtg aatacgtgtc gagtggtggc gtgatgcgtg 5941 aaacgtgaaa cgtgatgcgt gagattgaga gattgggaga ctgggagatt ggctggcccg 6001 acttacggca gggtgcctcg ctcgacctca cgcttcacgc ttcacgcatc acgccgagga 6061 acccatccat gcagaaaacc gatgatcgct atgcgcaata ccggggcggg cgcttcaact 6121 atcgtttgac cggcatcagc caggcgcctc tgtccgacat ctactatttg ctgatttcca 6181 agccgtggcc gtggttgttg gctacggcgt ttgtcggtta tctggtcatc aacggcggtt 6241 tcgctctgct ctactggctg ggcggcgacg gcatccaggg cgcgcagccg ggctcgtttc 6301 tggatgcgtt ctacttcagc gtccagacct tttccaccgt gggctacggc gtgctcagcc 6361 ccaaaacacc ctacgcccat gcgctggcga cggcagaatc cttcgcgggt atgttggcag 6421 tggccgtggg aacgggcatc atcttcgcca agttctcgcg cccgtcggcc cgggtgacgt 6481 tcagcgaagt gatggtggtg catccccgca acggcgtgcc caccttgcag ttccgcgtgg 6541 ccaaccagcg gcgcaacgaa atattcgccg cgcacatgaa tgtgcacgtg ctcatggagg 6601 agaccacggc cgaagggcac cggatgcgcc gcatgcacaa gttggtcttg gagcgagagg 6661 agatgccggt cttcaccgtg gcgtggaatg cgctgcaccc gctggacggc gccagcccgc 6721 tctacggcat gaatgccaac gtctgcaacc aaaatcttgt gttcatcgtc gtcgccttca 6781 gcggcaccga cggcaccttc ctacagacgg tccaggcgcg ccatctctac atgtccaagg 6841 acgtgcgctt cgacagccag tttgtggaca tgattgaaaa cggcaaggac ggcggcatgg 6901 tggtccacca cgaactgctg catgcgacac agccgttcga gcaaaacggt tccacaatgg 6961 cactgcctgt ggccgcggaa aaatcagccg cggagacgcc gcagcagtga gatgccccgt 7021 ggcatcagct cgttgtacgc ggtgtagagt //