LOCUS JACFMX010000084 10650 bp DNA linear ENV 05-AUG-2021 DEFINITION MAG: Rhodocyclaceae bacterium isolate BROCD009 NODE_3745_length_10650_cov_34.177253, whole genome shotgun sequence. ACCESSION JACFMX010000084 JACFMX010000000 VERSION JACFMX010000084.1 DBLINK BioProject: PRJNA647942 BioSample: SAMN15615530 KEYWORDS WGS; Metagenome Assembled Genome; MAG. SOURCE Rhodocyclaceae bacterium (wastewater metagenome) ORGANISM Rhodocyclaceae bacterium Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Rhodocyclaceae. REFERENCE 1 (bases 1 to 10650) AUTHORS Pabst,M., Grouzdev,D.S., Lawson,C.E., Kleikamp,H.B.C., de Ram,C., Louwen,R., Lin,Y.M., Lucker,S., van Loosdrecht,M.C.M. and Laureni,M. TITLE A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium JOURNAL ISME J (2021) In press PUBMED 34341504 REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 10650) AUTHORS Pabst,M. TITLE Direct Submission JOURNAL Submitted (25-JUL-2020) R&D, SciBear LLC, Tallin 13417, Estonia COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: MAR-2020 Assembly Method :: SPAdes v. 3.13.0 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 51x Sequencing Technology :: Illumina HiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 07/28/2020 14:11:42 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 4.12 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 3,401 CDSs (total) :: 3,355 Genes (coding) :: 3,310 CDSs (with protein) :: 3,310 Genes (RNA) :: 46 rRNAs :: 1, 1 (5S, 23S) complete rRNAs :: 1 (5S) partial rRNAs :: 1 (23S) tRNAs :: 40 ncRNAs :: 4 Pseudo Genes (total) :: 45 CDSs (without protein) :: 45 Pseudo Genes (ambiguous residues) :: 7 of 45 Pseudo Genes (frameshifted) :: 10 of 45 Pseudo Genes (incomplete) :: 26 of 45 Pseudo Genes (internal stop) :: 5 of 45 Pseudo Genes (multiple problems) :: 3 of 45 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..10650 /organism="Rhodocyclaceae bacterium" /mol_type="genomic DNA" /submitter_seqid="NODE_3745_length_10650_cov_34.177253" /isolate="BROCD009" /isolation_source="granules from the full-scale anammox reactor of Dokhaven-Sluisjesdijk wastewater treatment plant, further enriched at TU Delft in a chemostat over two months prior to sampling" /db_xref="taxon:1898103" /environmental_sample /geo_loc_name="Netherlands: Rotterdam" /lat_lon="51.5521 N 4.2845 E" /collection_date="May-2018" /metagenome_source="wastewater metagenome" /note="metagenomic" gene <1..199 /locus_tag="H3C33_15090" CDS <1..199 /locus_tag="H3C33_15090" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=2 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862414.1" /translation="AVLCALAALGLRLGIMGPPHFGGREPLSLDNHDYLWLVLGHLMA VLACAWLARRLFVLVRSSGDR" gene complement(177..1394) /locus_tag="H3C33_15095" CDS complement(177..1394) /locus_tag="H3C33_15095" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862415.1" /translation="MSDRYEHHFHEHTVEQYDNLLTLARYLVVNAGKIDAAGGLLFDR AYMLDFEPMPEGIAAHPPAAMPPLAYGPPAGIVALPGEDWQDYCQRAFGIEIRPYDPF CLWLQSPLWARTEPSALGAGLRIVYVLDLGVPHDHVEISMGQAWTDYDASGFLWDKLD MVPDAGRLPLRQWPEWIGAPTLNARRASITLNASNLRQTLIEHGIARPEDIAGLSEEE IRQLEFTTGALPRSYRQVLSVIGRRAGRLVDDRELQIYADQLPEVNRMGRERRADWEA EGGDPIPDDAIFIGARYGMSPWFILQDSRAHHPPRGDSPVFLFDTDTGKVTQVSISVW GWVEGLVRDAEIFIAEGIPEKNARRGHRTELPPLALQYKQQAQRDRNITIIMMAGAVL LGATLYLIYRLTT" gene complement(1406..1576) /locus_tag="H3C33_15100" CDS complement(1406..1576) /locus_tag="H3C33_15100" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862416.1" /translation="MTSRRTLTIAALVLLGILLAGFAALGFCDIYQNHIIRDCGPDCD APPSDGTVEGGF" gene complement(1599..2312) /locus_tag="H3C33_15105" CDS complement(1599..2312) /locus_tag="H3C33_15105" /inference="COORDINATES: protein motif:HMM:NF024824.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="tetratricopeptide repeat protein" /protein_id="MBW7862417.1" /translation="MSGIMLAVIVALPAHAQLPFTLRDCLADRDEDARLHICSIMSGT DEEKALGAVEASRILRARGEYDAAVDLLESSRGSRALLYPELGHVWFDQGDHMMADLH FEMALERGYEPDALTRERMVIAAHMYGEELQYSSESFAGAIGAYSRALELDPDYMTSL LGRAEASGVLGRHEEALADLDRAIALGADWTGYLLRARTRQELGDAAGAIADFRRVLE ENPQHTEAREALAGSGTLP" gene complement(2354..2662) /locus_tag="H3C33_15110" CDS complement(2354..2662) /locus_tag="H3C33_15110" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862418.1" /translation="MSRILISTLLVGATLLTAGCANSVLNDPEEMARRQAFVERQQAG WDRFWGGGSGGGSTASSSGSSTSQRTSPGLMQTSISTPSQCRAIGGTWNTASNYCWKL " gene complement(2749..3369) /locus_tag="H3C33_15115" CDS complement(2749..3369) /locus_tag="H3C33_15115" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862419.1" /translation="MTNAREQSQHGHAFRPGPFHRRRAYWLDGAMLHWRIGSSEGHVA LTDIASMRLNLAERTGAASRCMLVEKTGRVHKLSDIYWPRWTKEERRYWGRLQRRDAT FRGLTFTLARRLKKANPDAVIQTGPGRGEWLATCIVAAFAVAIVIGGAALMVVHSRFE PAATAFMALAAIYLPMIWPVIRSGGPKPLDPETLHNANPPPDWVDW" gene complement(3388..3882) /locus_tag="H3C33_15120" CDS complement(3388..3882) /locus_tag="H3C33_15120" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862420.1" /translation="MKIRPTILPLILFPTLAAPAFAEIPADGEGIAGLYAGSYMCQDG EHGVVLDIEMSEIEGRSELRLTGTLGFVPVLAGANGELADVAGSFTISGFLLTEPRGA NTAGYLQFQREEWLLEPEGYGSANFRGQLNRRDDGLWQITGTPLAGPTSEFCSDLIAT RFLP" gene complement(3895..4641) /locus_tag="H3C33_15125" CDS complement(3895..4641) /locus_tag="H3C33_15125" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862421.1" /translation="MLMALAPGTSKSAQAEEFAPDLHGCAALYDTLDKAKKSQQQMAL RLNSLYGFAEEDARWRDAGAFWAFSNARQIDFGERREALKERSGESDLAFDIQVLGIS GRPRFPNPGSAEAGFPQGAVLHNTQAPPASATPPYGHRISTCDREHGFWPVFYFGATD HLQCVAHYWTLGALRPDLQSETTPRVRHAAQAYARETPTATEAGIREDVLPRVQARGQ RIQTGEENPAALVADARACDARYGLEPLES" gene complement(4770..5174) /locus_tag="H3C33_15130" CDS complement(4770..5174) /locus_tag="H3C33_15130" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862422.1" /translation="MSMKAFAVILAMSIGVSAGPAKALERAEAHLDCLGLYALLRMAA PNFADWAEDRMAHTRQAYFRDLPFPSPVPMPLTSGEMEAETRVRMQARGARLADADSD EDVSRETDALLADIHACDARYDLPPTPTPWRD" gene complement(5185..6018) /locus_tag="H3C33_15135" CDS complement(5185..6018) /locus_tag="H3C33_15135" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862423.1" /translation="MKRKVMMVFAAMLTAAAFAVMPQEARADETREEAGRCAVRYLQV NHSTNASRIIAGYEAIDLRDRAWQLIRTHELAPAISTGPSTFAEDLNNFARGQASYLQ LGRMRDITLLPELPLQADIDSGDTDLQESAVSRLADCDRTFGFAPVIALAPAHAISDF DCAAAYWLRGAMYPMDQAAAGERAQFALGRYQAENAGLSVEQIVGQVQAAGNAHYERI MHESRGLEELRQEMIADGQNPDEAAFTERLQRRAVEALSFQQDIVACEAKYGFAQAGG Q" gene complement(6056..6733) /locus_tag="H3C33_15140" CDS complement(6056..6733) /locus_tag="H3C33_15140" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862424.1" /translation="MAAMRTGLAGLAAAALSVLALAAPGEARAENEDRSNDAQYVLNC AIEYAKSASADSSFLERQMALRDRFDLDHENIWLMAKLGDVSDIIPVEWIVDGLAECD RTFGFSPITELIDSRPGIPLTPSSPQIADFDCAVAYRLLAAIRHDVDQDQVQAQNAMQ RAEYALRLHQTANPELDPGSLGPQVNGLARTRGEQIQQGQESGEALTNDINACEAKYG FAQAGGQ" gene 6930..9134 /locus_tag="H3C33_15145" CDS 6930..9134 /locus_tag="H3C33_15145" /inference="COORDINATES: protein motif:HMM:NF014567.1,HMM:NF019350.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="ATP-binding protein" /protein_id="MBW7862425.1" /translation="MLLFSRFTPNVLLSAAVALSVILAAVMLGLAIDQRWLGLKLEAQ PRLAVPVSFEGLWIDAIEHDSPAAGQPGAGIPASVASLVSLGGPDGRRIQLTPQDLME EPDALETYAGMRAFFARQDEIAAVLRSIGGVELGIAFLGETQTYSVSPKRQRPVASLP PSFWMQIGVGLAGFWIGAWIWSLRRGEWATRFLFLAGAGLMISAFPAAVYSTRELALP GGLFRVLSTFNHIGALTFGVGMIGLFLVYPRTLVSPRWLALPAIVLGAWQIVDILQLA NGPGLGFHLAVVLAMLAIVVLVVIQFRVTRGRPRDRAALAWLGLAVIVGAGAFVTTVI APTALGIGAFVSQGEAFLFFLLVYVGVALGVARYRLFQLDEWAFRILFYVVGVLLLLV LDAILIVTIIDERTPAFALSLLIVALAWLPLRDMLARIVLRRADPSRGILFRKIMDVA LTPPGRDQQARWRALLEDTFRPLSITTATDVQAPALIDDGLALAVPGPGGLPGYRLDY ADGGRKLFSLRDADLAVELTTMLANALESREAYEKGVAEERERIARDIHDNIGVQLMG ALHSRGLARKDMMIRETLTDLRDIINNASNPDLSFDEMLADLRSQISEHLFAAGVKMK WEVETAGPAILPLAAAHTVRSVVREAVQNALKHGQPEAISVTIRLDEDAIALTVADDG GGFDPSSVQTGNGLANMQARVTSLGGRINIASGAEGTRIEAQFPVDIVRITQ" gene 9131..9790 /locus_tag="H3C33_15150" CDS 9131..9790 /locus_tag="H3C33_15150" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008333850.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="response regulator transcription factor" /protein_id="MBW7862426.1" /translation="MTKILIVEDLSETRRWLSEIVSAAFEQSEIHEAASMRAGIAQAA ARDYDVALIDLGLPDGSGLDVLRNLRLLRPQTVCVVTTVMGDDASIVGALSAGAQGYL LKEQPQGLLVRQLKQMAEGIPALSPSVARRIMEHFKRTGPAAPEDDLTGREREVLGLI GRGLRNGEVAETLGISENTVAGYIKDIYRKLGISSRAEASWHAARLGLGEAPAANKKS K" gene complement(9906..10127) /locus_tag="H3C33_15155" /pseudo CDS complement(9906..10127) /locus_tag="H3C33_15155" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_012112836.1" /note="incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology." /pseudo /codon_start=1 /transl_table=11 /product="N-acetyltransferase" gene complement(10258..10344) /locus_tag="H3C33_15160" /pseudo CDS complement(10258..10344) /locus_tag="H3C33_15160" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005854552.1" /note="incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology." /pseudo /codon_start=1 /transl_table=11 /product="ATPase" gene complement(10467..>10650) /locus_tag="H3C33_15165" CDS complement(10467..>10650) /locus_tag="H3C33_15165" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014131760.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=2 /transl_table=11 /product="hypothetical protein" /protein_id="MBW7862427.1" /translation="SQWKLGTEPGHVSMEINTFGSERLTGALLDRLTHHVSILEMNGE SYRLAQSRAKGAKPHQ" BASE COUNT 1805 a 3345 c 3437 g 2063 t ORIGIN 1 cgctgtgctt tgcgccctcg cggcgctcgg cctgcggctc ggtatcatgg ggccgccgca 61 tttcggggga cgggagccgc tgtcgctcga caaccacgat tacctctggt tggttttggg 121 ccacctgatg gctgtgctgg cctgcgcctg gctcgcgcgg cggctgttcg tgctggtcag 181 gtcgtcaggc gatagatgag gtaaagggtt gcaccgagca gcactgcgcc cgccatcatg 241 atgatggtga tattgcgatc cctttgcgct tgttgcttgt attgcagcgc caggggcgga 301 agttctgttc gatggccccg gcgggcgttc ttctcgggga tgccctcggc gataaagatt 361 tcggcatcgc ggaccagccc ctcgacccag ccccagacgc tgatcgagac ctgtgtgacc 421 tttcccgtgt ccgtatcgaa caaaaagacc ggggaatccc cgcgaggggg gtggtgggcg 481 cggctgtcct gcaggatgaa ccagggcgac atgccatagc gcgcgccgat gaagattgcg 541 tcgtcgggga tggggtcgcc accctccgcc tcccaatccg cgcgcctttc acgtcccatg 601 cggttcactt cgggcaactg atcggcgtag atctggagct cgcggtcgtc caccagccga 661 ccggcacggc gaccgatcac ggaaagcacc tgccggtagc tgcgcggcaa cgcgccggtc 721 gtgaattcga gctgacggat ttcctcttca ctcagcccgg caatgtcctc gggccgggcg 781 atgccgtgtt cgatcagcgt ctggcgcaga ttgctcgcat tgagcgtgat tgaggcacga 841 cgtgcgttta gcgtgggcgc gccgatccat tcgggccatt gacggagggg caacctgccg 901 gcatccggca ccatatcgag tttgtcccac agaaaaccgc tggcgtcata atcggtccag 961 gcctgaccca tggaaatctc gacatgatcg tgcggcacgc cgagatccag cacatagacg 1021 atcctcaatc cagcgcccaa ggcgctcggt tcggtcctgg cccaaagagg cgattgcagc 1081 cagaggcaga aggggtcgta gggtcggatt tcgatgccga aagcgcgttg gcaatagtct 1141 tgccaatcct cgccgggcag cgccacgatg ccggccggcg gaccataggc cagcggcggc 1201 atggcggcag ggggatgagc ggcaataccc tcgggcatcg gctcgaaatc cagcatatag 1261 gcgcgatcga acagcagccc gccagcggcg tcgatcttcc cggcattgac gaccagatag 1321 cgcgccagcg tcagcagatt gtcgtactgt tcgacggtgt gctcatggaa atgatgctcg 1381 tagcgatcgc tcaccctgcc accgctcaaa agccgccctc aacggttccg tcggacggtg 1441 gagcgtcgca atccgggccg caatccctga tgatgtggtt ctggtaaata tcgcagaacc 1501 ccagcgcggc gaacccggcc agaaggatgc cgagcagaac gagggccgcg atggtcagcg 1561 ttcttcggga ggtcatgtgc gaaggctcct ttgttgtgtc agggcagggt gccggacccg 1621 gccagcgcct cgcgagcttc tgtgtgctga ggattttcct caagcacgcg tcggaaatcg 1681 gcgatggcgc ccgccgcgtc gccgagctcc tggcgggttc gggcgcgcag aagatagcct 1741 gtccaatccg cgccgagcgc gatcgcccgg tcgagatcgg cgagcgcctc ttcgtgtcgg 1801 ccgagtaccc ccgaggcttc ggcccggccg agcaggctgg tcatgtaatc cggatccagt 1861 tccagcgcgc gggaataggc gccgatggcg ccggcgaaac tttcactcga atattgcaat 1921 tcctcgccat acatatgcgc cgcaatgacc atgcgctcac gtgtcagggc atcgggctca 1981 tagcctctct caagcgccat ctcgaaatga agatcggcca tcatgtggtc gccctggtcg 2041 aaccagacat ggccgagttc gggataaagc aacgcacggc tgcccctgct gctttcgagg 2101 agatcgacag ccgcatcgta ttcgccgcgt gcgcgcagga tgcgcgatgc ctcgaccgca 2161 cccaacgcct tttcctcgtc ggtgccggac atgatcgagc agatatggag cctggcgtcc 2221 tcgtcccgat cggcgaggca gtcccgcagg gtgaagggca gttgggcatg tgcgggcagc 2281 gcgacgatga cggcgagcat gatgccggac aagcgcgacc atgctccgcc aaaggtgcgg 2341 ccagtgcgag tcatcagagt ttccagcaat aattgctcgc ggtgttccag gtgccgccaa 2401 tggcacggca ttggctcggc gttgaaatcg aggtctgcat gagaccggga ctggttcgct 2461 gcgacgtgct gctccctgag cttgacgcgg tactgccgcc accggaaccg ccaccccaga 2521 acctgtccca gccggcctgt tgccgctcga caaacgcttg gcggcgggcc atctcctcgg 2581 ggtcgttgag aacgctgttg gcgcagccgg cagtaagaag cgttgcgccg acgagcagcg 2641 ttgagatcag gattcgtgac attgctccct ccaatctgga ctttgagtca tcgtgccggg 2701 gatgcgcgga tggttctccc ccctgttcag gggggatgga acgccaagtc accaatccac 2761 ccaatcgggt ggcgggttgg cgttgtgcag ggtttcgggg tcgagcggtt tggggccgcc 2821 ggatcggatg accggccaga tcatcggcag atagatcgcc gcgagggcca tgaaggcggt 2881 ggcagcaggt tcgaacctgg aatgcaccac catcagcgcg gcgccgccga tgacaatggc 2941 gacggcgaac gcggccacta tgcaggtggc gagccattcg cctcggcctg gtccggtttg 3001 gataacggcg tcggggttgg ctttcttcag tcgccgcgcc agggtgaagg tcaggccccg 3061 gaaggtcgca tcgcgccttt ggagccgacc ccagtatcgg cgctcttcct tcgtccagcg 3121 cggccagtag atgtccgaga gcttgtggac ccggcccgtt ttctcgacca gcatgcaacg 3181 cgacgccgcg ccggtccttt cggccaggtt gagtcgcatg gacgcgatat cggtgagcgc 3241 cacatgaccc tcgctggaac cgatacgcca atgcagcatg gccccgtcca gccagtaggc 3301 acgtcgccgg tgaaacgggc cggggcggaa ggcatggcca tgctggctct gttcgcgggc 3361 gttggtcatc gcgccaatcc cccccgttta cggcaggaac cgggtggcga tcagatcgga 3421 gcagaattcc gatgtcggcc cggccagggg cgtgccggtg atctgccaca acccgtcgtc 3481 gcgccggttc aactggccac ggaagttggc tgagccgtac ccctcgggct cgagcagcca 3541 ctcttcgcgc tggaactgga gatagccggc agtgttggcg ccgcgcggct ctgtcaggag 3601 aaaccctgag atcgtgaaac tgcccgcaac gtccgcaagc tcgccattcg ctccggcgag 3661 caccggaacg aatcccaagg tgccggtgag gcgcagttcg gatcggccct cgatttcgct 3721 catttcgata tcgagcacaa cgccatgctc tccgtcctgg cacatatagc tgccggcata 3781 gagaccggcg atcccttcgc catcggcggg gatttcggcg aaggccggcg cggccagcgt 3841 ggggaacagg ataaggggca ggatggtggg tctgattttc atggggcatt tctcctatga 3901 ttcgagcggt tccaggccgt atcgcgcatc gcaggcccga gcgtcggcga cgagggcggc 3961 ggggttttcc tcgcccgtct gaatccgttg cccgcgcgcc tgcacgcgcg gcagcacgtc 4021 ctcacgaatg ccggcttcgg tcgccgtcgg cgtttccctg gcataggcct gcgcggcatg 4081 ccgcacacgc ggtgtcgtct cggactggag atcggggcgg agtgcgccca atgtccagta 4141 atgcgcgaca cactggaggt gatcggtggc gccgaagtaa aacacgggcc agaacccatg 4201 ctcccggtcg caggtgctga tccggtggcc gtaagggggt gtggcgctcg cggggggcgc 4261 ttgcgtattg tgcaagaccg cgccttgggg aaagccggcc tcggcgcttc cggggttggg 4321 aaagcggggg cgtccggaaa tgccgagcac ctggatatcg aaggcaagat cgctctcgcc 4381 ggacctctcc ttcaacgcct cccttcgttc accgaaatcg atttgacggg cattgctgaa 4441 cgcccagaac gcgccggcgt cccgccaccg cgcgtcctct tccgcaaatc cgtacaaaga 4501 gttcaatctt agggccattt gttgctgcga tttcttagcc ttgtcgagcg tgtcatagag 4561 cgccgcgcag ccatggagat caggggcaaa ttcctcagcc tgggctgatt tcgacgtacc 4621 gggcgcaagc gccatcagca tcgcggcgca agctgccgtc caccctcctc tcatcgcatt 4681 cgctcctttc cttcgccgat tgtctgatca tccgttgcgg cagaatcggc gaggcggaag 4741 gtcggcgcac ccccctgttc aggggggtat caatcccgcc agggtgtcgg cgtcggggga 4801 agatcgtagc gggcgtcgca ggcgtgaatg tcggcaagga gggcgtcggt ttcgcgcgac 4861 acatcttcgt cggaatccgc gtctgcaagt cgtgcccccc gcgcctgcat tcgcacgcgg 4921 gtctcggctt ccatttcgcc gctggtcagt ggcatgggca cgggcgaggg aaagggcaga 4981 tcgcggaaat atgcctgccg ggtatgggcc atgcgatctt cggcccaatc ggcgaagttc 5041 ggtgccgcca tcctgagcag ggcatagagg ccgaggcagt cgaggtgggc ctcggcgcgt 5101 tcgagtgcct ttgccggccc ggccgaaacg ccgatggaca ttgccaggat gaccgcgaaa 5161 gccttcatgc tcatcccggt gcgcctactg tccgccggcc tgggcaaagc cgtatttcgc 5221 ctcgcaggcg acaatatcct gctgaaaact cagcgcctcc accgcccggc gctgcagccg 5281 ctccgtgaag gcggcttcgt cggggttctg tccgtcggcg atcatctcct gccgaagctc 5341 ctcaaggccg cgggactcgt gcatgatgcg ctcgtagtgg gcgttgcctg cggcctgcac 5401 ctgcccgacg atctgttcca ccgataagcc tgcgttttcc gcttgatagc gcccgagcgc 5461 gaattgcgcg cgttccccgg cagcggcctg gtccatcggg tacatggcac cgcgaagcca 5521 ataggcggct gcgcagtcga aatcgctgat cgcatgcgcg ggggcaagcg cgatcactgg 5581 cgcaaagccg aacgtccggt cgcagtcggc gaggcgcgaa acggcgcttt cctgcaggtc 5641 tgtatccccg gaatcgatat ccgcctggag agggagctcc gggagaagcg tgatatcccg 5701 catgcgtccg agctgcaggt agctggcctg cccccttgca aaattgttga ggtcttcagc 5761 aaaggtggat ggtccggtcg aaatggcagg cgccagttcg tgcgtccgga tcaattgcca 5821 cgcccgatcc cgcaaatcga tggcctcgta tccggcaatg atccggctcg cgttggtgga 5881 atgattcacc tgtaagtagc gcacggcaca gcgcccagct tcctctctgg tttcatccgc 5941 gcgcgcttcc tgcggcatta cggcgaaggc cgccgctgtc agcatcgcgg caaaaaccat 6001 catcactttc cgtttcatcg cggttcccca ctttcatgcc caccacatgg cgcgcctact 6061 gtccaccggc ctgcgcgaag ccgtattttg cctcgcaggc attgatgtcg ttggtcagcg 6121 cctcgcccga ttcctgaccc tgctgaatct gctcgccgcg tgtccttgcc agcccgttca 6181 cctgcgggcc aaggctgccg gggtccagtt cggggttggc ggtctggtga agccggagcg 6241 catattccgc gcgctgcatc gcgttctgag cctgcacctg atcctgatca acatcatgcc 6301 ggatcgcggc aaggagccga taagcgacag cgcagtcgaa atcggctatc tgaggactgg 6361 agggggttag aggtatgccc ggtctcgaat cgatcaattc ggtgattggg ctgaacccga 6421 atgtccggtc acattcggcc agaccgtcta caatccattc tacgggtatt atatcggaga 6481 catctccgag cttggccatg agccagatat tttcatgatc gagatcgaaa cgatctctca 6541 acgccatttg ccgctccagg aaacttgaat ccgccgaagc actctttgca tattcgatgg 6601 cacaattcag gacgtactgg gcatcgttcg atcggtcctc gttctccgcc cgtgcctctc 6661 ccggcgcggc cagcgccagc acggacagcg ccgccgccgc caggcccgcc aatccagtcc 6721 gcatcgccgc catatccgtt tcctccgccg gtgatctctg cctctgacca caggactaga 6781 cccgcgcgcg cagggcagca cccccctgtt caggggggtt atggctaaga ggtgcggcaa 6841 taggttaggt aggagctgaa cggttgaacc caatctgctg ccttcagccg cttgaacctt 6901 gacccgcttt gcctcgccac aggagacaca tgctcttgtt ttcccgcttc acccccaacg 6961 tcctgttgtc cgccgctgtc gcgctcagcg tcattctggc ggcggtgatg ctgggcctgg 7021 cgatagatca gcgctggctg gggctcaagc tcgaggctca acccaggctc gccgttccgg 7081 tcagcttcga gggattgtgg atcgacgcga tagagcatga cagcccggcg gccgggcagc 7141 cgggcgcggg gattccggca tccgtcgcct cgctggtgag cctgggcgga cccgatggga 7201 gaaggatcca actgacgcct caggacctca tggaggaacc ggatgcgctc gagacctatg 7261 ccggcatgcg cgccttcttt gcccggcagg atgagattgc cgcggttctg cgcagcattg 7321 gcggggtcga gcttgggatc gcgttcctgg gcgagacaca aacctattca gtctctccca 7381 agcgccagcg cccggtcgcc tctttgccac cttccttctg gatgcagatc ggcgtggggc 7441 tggcgggttt ctggatcggg gcgtggatat ggtcgctgag gcgtggcgaa tgggcgacac 7501 gcttcctgtt tcttgcgggc gcaggcctga tgatttcggc ctttccagcc gccgtctact 7561 cgacccggga gctggcgctt cccggcgggt tgttccgggt tctctctaca ttcaaccata 7621 ttggggcgct cacattcggc gtgggcatga tcgggctgtt tctggtctat ccccgaacgc 7681 tggtatcacc ccgttggctc gcgcttcccg ccattgttct cggcgcgtgg caaatcgtgg 7741 atatccttca actggcgaac gggccgggcc tgggtttcca tctggcggtc gttctcgcca 7801 tgctcgccat cgtggttctg gttgtcatcc agttcagggt cacgcgcggg cgcccgcgcg 7861 accgtgcggc cctcgcttgg ctcgggctgg cggtcatcgt cggcgcgggc gccttcgtca 7921 cgacggtcat agcgcccacc gcactgggga tcggcgcctt cgtctcgcag ggggaagcgt 7981 tcctgttctt cctgctggtc tatgtcggtg tcgcgctcgg ggtcgcgcgg tatcggctgt 8041 tccagctcga cgaatgggcc ttccgcattc tcttttatgt cgtcggcgtg cttctgcttc 8101 tcgtgctcga tgccatcctg atcgtcacca tcatcgacga acgcacaccg gccttcgcgc 8161 tgtcgctgct gatcgtggct ctggcctggc tgcccctgcg cgacatgctg gctcgaatcg 8221 tgctgcgccg cgcggacccg tcgcgcggaa tcctgttccg taagattatg gacgtggcgc 8281 tcactccgcc gggccgcgac cagcaggcgc gctggcgcgc gcttctggaa gataccttcc 8341 ggccgctctc gattacaacc gctacggatg tgcaggctcc ggcactgatc gatgatggtc 8401 tggcgcttgc cgtgcctggt cccggtggtc tgccgggcta tcggctggat tatgcggacg 8461 gaggacgaaa gctgttctcg ctgcgcgatg cggatttggc ggtcgaactc accaccatgc 8521 tcgccaatgc gctggaaagc cgggaagcct atgaaaaggg cgtcgccgag gagcgtgagc 8581 gcatcgcccg cgacatccac gacaatatcg gtgtgcagct gatgggcgcg ctgcacagcc 8641 gggggctggc gcgcaaggac atgatgatcc gcgagacgct gactgatctg cgcgacatca 8701 tcaacaacgc ttccaatccc gacctctcct ttgacgagat gctggccgat ctgcgctcgc 8761 agatctccga gcatctgttc gccgctggtg tgaagatgaa atgggaggtg gagactgccg 8821 gacccgcgat cctgccgctg gcggccgcgc atacggtgcg ctcggtcgtc cgcgaggcgg 8881 tgcagaacgc gctcaagcat gggcagccgg aggcgatcag cgtcacgatt cgcctcgatg 8941 aagacgccat agccttgacc gtggccgacg atggcggcgg tttcgatccc tcaagcgtcc 9001 agaccggcaa cgggcttgcc aatatgcagg cgcgcgtgac gagtctgggt ggccggatca 9061 acatcgcaag cggggcagag ggaacccgta tcgaggcgca gttccccgtt gatattgtac 9121 ggattacaca atgacgaaaa tactcatcgt cgaggacttg agcgaaaccc gccgctggct 9181 gagcgagatc gtcagcgcgg ctttcgagca gtccgagatt cacgaagcag cgagcatgcg 9241 cgccggcatc gcgcaggcag cggcgcgcga ctacgatgtc gcgctgatcg atctcggcct 9301 gccggacggg tccggcctcg acgtattgcg caatctgcgc ctcctgcgcc cccagactgt 9361 ctgtgtcgtc accaccgtca tgggcgacga cgcctcgatc gtgggcgcgc tttcggcagg 9421 cgcgcagggt tatctgctca aggaacaacc ccagggtctg ctggtccggc agctcaagca 9481 gatggccgag ggcattcccg cgctctcacc ctcggtcgcg cgccggatca tggagcattt 9541 caagagaact ggcccggccg cgcctgaaga cgatctgacg ggacgcgagc gcgaggtgct 9601 tggcctgata ggccgcggcc tgcgcaatgg cgaggtggcc gagacgttgg ggatttcgga 9661 aaacacggtg gcgggctaca tcaaggacat ctatcgcaag cttggtattt cctcgcgcgc 9721 cgaagcctcc tggcatgcgg caaggctggg cctcggcgaa gcgcccgccg ccaacaagaa 9781 gagcaagtaa cgtcctgctc cggattctac gcgctttttt ggattggcgc gcttgatccc 9841 gcctttcttc aggggtgaag acgaatttga atggcggatc gctacggttg gagccagcca 9901 accgttcagc cggcagcctc gccgagcgac gctgcgatgt cgctgatcga gcgaagcaga 9961 accaggggat catccctgga cggctcaaag ccgcgccgcc tccagaactc ggccgcctcc 10021 ccgtcgacgg cgttgaccat caaggcccgc ccgccgatca gcgacgcggc ctgaacacag 10081 cgctggaggg cgtgtttcac aaggccggtg ccaataccca accctgcctg tcaatcggca 10141 gccaatcgtg accccctatc ggcatccaaa aatgaccccc ttggagcgcc cgggtagctg 10201 gcccgatgcg gtgtagccct cacacagcgc agccgtatcg ggccagcggg ttctcggtca 10261 ttcacgggtc ttgaagcgcc aactctcgtt gccggtttcg acgatgtcgc agtgatgggt 10321 caggcggtcg agcagcgcag tggtgtcaac ggcggagtaa aagtcgtcca ttgggcggcg 10381 caaaagtagg ccacttggcg ctgcaagcgg ggaacgtcgg gagggcgtag cccgaccaga 10441 gtttcccgct tgcagcgtcg gcgatcctat tggtggggct ttgcgccttt tgcccggctt 10501 tgcgccagtc ggtagctttc gccgttcatc tcaaggatgc tgacgtgatg ggtcagccgg 10561 tcgaggagcg cgccggtgag acgctcggac ccgaacgtgt tgatttccat cgagacgtga 10621 cccggttcgg ttcctaattt ccattgagat //