LOCUS JACCVK010000183 9935 bp DNA linear ENV 26-JUL-2020 DEFINITION MAG: Actinomycetota bacterium isolate MGR_bin124 SD2897-2912_k127_3943016, whole genome shotgun sequence. ACCESSION JACCVK010000183 JACCVK010000000 VERSION JACCVK010000183.1 DBLINK BioProject: PRJNA630822 BioSample: SAMN15052007 KEYWORDS WGS; ENV; Metagenome Assembled Genome; MAG. SOURCE Actinomycetota bacterium (soil metagenome) ORGANISM Actinomycetota bacterium Bacteria; Bacillati; Actinomycetota. REFERENCE 1 (bases 1 to 9935) AUTHORS Leung,P.M., Ortiz,M., Shelley,G., Cowan,D.A. and Greening,C. TITLE Metagenome from Mackay Glaciers Regions JOURNAL Unpublished REFERENCE 2 (bases 1 to 9935) AUTHORS Leung,P.M., Ortiz,M., Shelley,G., Cowan,D.A. and Greening,C. TITLE Direct Submission JOURNAL Submitted (01-JUN-2020) Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Melbourne 3168, Australia COMMENT The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 2020 Assembly Method :: Megahit v. 1.2.9 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 8.18698908481168x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 07/15/2020 18:19:29 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 4.12 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 2,377 CDSs (total) :: 2,348 Genes (coding) :: 2,321 CDSs (with protein) :: 2,321 Genes (RNA) :: 29 rRNAs :: 1 (16S) partial rRNAs :: 1 (16S) tRNAs :: 26 ncRNAs :: 2 Pseudo Genes (total) :: 27 CDSs (without protein) :: 27 Pseudo Genes (ambiguous residues) :: 0 of 27 Pseudo Genes (frameshifted) :: 12 of 27 Pseudo Genes (incomplete) :: 15 of 27 Pseudo Genes (internal stop) :: 3 of 27 Pseudo Genes (multiple problems) :: 3 of 27 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..9935 /organism="Actinomycetota bacterium" /mol_type="genomic DNA" /submitter_seqid="SD2897-2912_k127_3943016" /isolate="MGR_bin124" /isolation_source="mineral soil" /db_xref="taxon:2900548" /environmental_sample /geo_loc_name="Antarctica: Mackay Glacier regions" /collection_date="2015-01" /metagenome_source="soil metagenome" /note="metagenomic" gene <1..257 /locus_tag="H0U46_05890" CDS <1..257 /locus_tag="H0U46_05890" /inference="COORDINATES: protein motif:HMM:NF015000.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=3 /transl_table=11 /product="hypothetical protein" /protein_id="MBA2741525.1" /translation="LGLHVLRPGMPFLARALLRRSRASALVLHHELVGEGLVRYAQRR GVPVVVWTVDEPRDVERLDRIGVDAMVVNNPVMFTSTLSV" gene 254..1315 /locus_tag="H0U46_05895" CDS 254..1315 /locus_tag="H0U46_05895" /inference="COORDINATES: protein motif:HMM:NF015679.1,HMM:NF023650.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="L,D-transpeptidase/peptidoglycan binding protein" /protein_id="MBA2741526.1" /translation="MKRGTLLVAAGMLAVCALLGLASGFALASTDIAPSQTTDPPPTT SPPPTETSPTPPPALPPKRQLIEPGVIVGGIAVGWLTSRTASDLVRKTFSRRLVLVAG PGQRVRIAPQDLGASARIEKAIRRASSARPGAQIPLEVVVSRSKLKSYVEKLAARFDR KAIDAGVVLSGIRPRFVEAQDGRQLRANRTGLALRISLATHMRDPIPLDFDVTKPEVR TTKVGSAIVILRDSKRLLLYSTKKMVRRFGIATGQSSYPTPTGDYEITTMQRNPWWYP PPSDWAAESDPVPPGPGNPLGTRWMGISAPYVGIHGTPDAASIGYSASHGCVRMRIPD AEWLFRRVEVGTPVFILSR" gene 1495..2055 /locus_tag="H0U46_05900" CDS 1495..2055 /locus_tag="H0U46_05900" /inference="COORDINATES: protein motif:HMM:NF020123.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="TlpA family protein disulfide reductase" /protein_id="MBA2741527.1" /translation="MSRRLLLVAQGTAIGLVVLLFALLLWKLVADEGGSIASATDRGE RPPAPEFTLERLDREGDLTLSSLRGKAVVLNFWASWCIPCKEEAPYLEEVWQANRDDG LVVVGLDAKDFRRDARAFAERFELTFPIVYDGPGSTLSSWGVQGFPETFVIDRRERVV ESFLGAVNSDEDRARLRAAIERALEP" gene 2052..2510 /locus_tag="H0U46_05905" CDS 2052..2510 /locus_tag="H0U46_05905" /inference="COORDINATES: protein motif:HMM:NF015853.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="cytochrome c-type biogenesis protein CcmH" /protein_id="MBA2741528.1" /translation="MKRFAPTLVLTIALGLLVPVSVWAADPPSAADLEAELVCPVCET TLDQSNAPVAERMKVFIRERIAAGDTEQEIKDALVAEFGSGVLATPGKRGFGLLAWLI PLAAIAVGALAVGLLVRGWTRRGVPADDDPAPLDAGLERRLDEELARFDG" gene 2512..3234 /locus_tag="H0U46_05910" CDS 2512..3234 /locus_tag="H0U46_05910" /inference="COORDINATES: protein motif:HMM:NF014713.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="cytochrome c biogenesis protein CcdA" /protein_id="MBA2741529.1" /translation="MDPGSALAAFVAGFVSFLAPCVLPLVPGYLSAISVLDASELGRP GHARRVIAASVPFVLGFTMFFVSLGVGAALVGGRLFSDQFLLEKVAGFVLVVFGLAFT GLLPWPERLVGARLVQGARGSGSRLFLGGAFAVCAAPCISPVLGGILVLAGSSGTVVE GALLLGVYSLGLAVPFVLVGVLFTRAMGAFRWLRDHYRVIQFVGGAIMVALGLLLFFE RFYVLRIYLNRFLLWLGLEPAF" gene complement(3231..3554) /locus_tag="H0U46_05915" CDS complement(3231..3554) /locus_tag="H0U46_05915" /inference="COORDINATES: protein motif:HMM:NF015690.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DUF309 domain-containing protein" /protein_id="MBA2741530.1" /translation="MGLDAGLELIGKGAYFEAHEELEDEWREAPGAERDFLQGLVHVA VAWLHAERRNGPGCERQLEKARRRLSPYAPFHRGVDVASLLAQVEAAERDVTAGHLDL HPVEV" gene 3597..3908 /locus_tag="H0U46_05920" CDS 3597..3908 /locus_tag="H0U46_05920" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBA2741531.1" /translation="MATILLAGVDLFFRGKLEGLLSAHKLVTSDSVSAPDLVIADIAR VEPEEVVEAWPDIPIVGFTNHTDRAGLQRAHAAGFDQVIVKSALVERAGALVDELIGV A" gene 3963..4265 /locus_tag="H0U46_05925" CDS 3963..4265 /locus_tag="H0U46_05925" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_011955081.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="ferredoxin family protein" /protein_id="MBA2741532.1" /translation="MTYIIAEPCIDIKDLSCVDVCPVDCIHVFERILIIDPEECIDCG ACEPECPVEAIFPEDALPDKWNAFVEINYAFPDAAKINPLVDKYAEENDVHNEPLE" gene complement(4272..4475) /locus_tag="H0U46_05930" CDS complement(4272..4475) /locus_tag="H0U46_05930" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBA2741533.1" /translation="MSAITNGTGGYLLFVWSPAGYRLVEQEGEVPLVGSEVENGERRY RVAKVASSPLPGDVRPCLYLLPS" gene complement(4489..5139) /locus_tag="H0U46_05935" CDS complement(4489..5139) /locus_tag="H0U46_05935" /inference="COORDINATES: protein motif:HMM:NF019350.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="MBA2741534.1" /translation="MSSDSERALVGERILAEQEERRRLAELIHDGPVQHVAAITQIVD SALAALRDGDRQDALELLTRGLALTREAGQDLRALCEDLEPRALRALGFASAVEALGR RVSSRRGVEVVLDVEHGDSLGEHAQTGLFQIVRDALEQAVRRGALTRIDVGLRGLEGG GAELLIADDGPPERRSAVLELLADRAATLNGRFASEARWPRGASIRVELPPSSARR" gene complement(5136..5747) /locus_tag="H0U46_05940" CDS complement(5136..5747) /locus_tag="H0U46_05940" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015805678.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="response regulator transcription factor" /protein_id="MBA2741535.1" /translation="MTCLIADDHEVVREGLRLALSRSPHIRVIGEATDGKSAVTLTER RRPDVVIMDLRMPDMDGLEATEQILEKMPGTAVLIFTAYGERSLMQRGLESGARGYIL KETPHETLIRAIEKVAAGDTFVDPALMAEFIASRGQLDVLTPREREILQLLADGMSNV DVAAKLFISQETVKSHVRHILAKLEADTRTQAVAIALREAMID" gene complement(5829..6728) /locus_tag="H0U46_05945" CDS complement(5829..6728) /locus_tag="H0U46_05945" /inference="COORDINATES: protein motif:HMM:NF015983.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="radical SAM protein" /protein_id="MBA2741536.1" /translation="MRVEYRMEPCKSALNPVRGMPFRWSLNPYMGCVHRCTFCYVPNF ERRADRPSDERYGRSIRVKTNVADVLARELARRSWVRDEVAIGTATDPYQPAEGRFRL TRACIVELEKAWTPFSIITRGPLAVRDIDVLLEASKRVEVSVTFSIPTLDERVWRTTE PGTAPPSSRLEAVRRIASAGVDVGVGLAPILPGLSDRREQLDAVVHAAKAAGARGVWA APVHLRPAVREHFLAALARDWPEHVTRYEELFAERAYLPSAIAAPIRERVRVAARATQ LSERYRRTERAREREPAQLSLVV" gene 6783..7724 /locus_tag="H0U46_05950" CDS 6783..7724 /locus_tag="H0U46_05950" /inference="COORDINATES: protein motif:HMM:NF012955.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="MBL fold metallo-hydrolase" /protein_id="MBA2741537.1" /translation="MAEPELPGGIRQVTRPLPTRPGHVHAYLLPGEDGWTIVDTGIGL PDAKESWRAELERAGGQVARIFVTHFHPDHVGAAADLHELTGAPVYQGTLDYAQCELV WGNPAWPERLIDWFRLHGTPDDVTEELVGQSSVYRPYIRYQPDPILVRAGEWLDGWEL VAAAGHADGQLCLLKDGVLLAADHVLERISPTVGLWPASRPDPLGDYLEALDRTVKIE PRIALPGHGELIADPAGRARELQEHHRIRLQEAVAAFGPKPLSGYELSLVLWGDVLKP AARRFAVAESLSHAERLVRVGAAVRHEVDGAVSYTAA" gene 7801..9102 /locus_tag="H0U46_05955" CDS 7801..9102 /locus_tag="H0U46_05955" /inference="COORDINATES: protein motif:HMM:NF012780.1,HMM:NF013741.1,HMM:NF015436.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="HlyC/CorC family transporter" /protein_id="MBA2741538.1" /translation="MSTSLRIVVIFLLVFANAIFVAAEYALVTARRSRLEERAVKGGL GARTALRLMDDPVRFISTVQIGITVSAILLGAIGEPLLSELMDPPLSNTVAFLIAFAI LTYLSVVVGELVPKAVALQKAEPIAVALAVPLDWIARFAHPVVWALQVSANVLLKGLR VKPAPTGMIAYTREDIRYSVAAAEDVGELHQAEEEMLYKVFDFASKEVSAVMVPRPEV VAISVDVTPEEALATILDSPYTRYPVFRESLDHIVGVLHVRDLFAAMHDQGIAAVELE AILRPGYVVPETKDLAALLADFRRKKQHQAIVVDEYGTMAGIVTLEDILEEIVGEIED EFDLPDESVERVDEKRIRIDGTFTIDDFNEQFATELEQDDYHTMAGLVFGTLGRQPEV GDSVAVNGLRLEVLEVDGSRIGRIEIEFDAEEQPAGAPEAA" gene 9118..>9935 /locus_tag="H0U46_05960" CDS 9118..>9935 /locus_tag="H0U46_05960" /inference="COORDINATES: protein motif:HMM:NF017760.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="MFS transporter" /protein_id="MBA2741539.1" /translation="MSSGARAVFSHRDFRLYWIAIFAEGFGLTMALVAIGWQVYSVRG NALDLGLVGLAEFLPLLLFALPAGQLADRVSRRRLLVVMVVLDIVMLGGLLVVTVSGA DEVWPFFALAFLFGTSSALGAPAGRALTPSLVPEELLVSALAQRSIVNQISLVSGPAV GGLLFAVRAELVYAIAIALALVSLVCFLSLRTGRMPAAEGVEGLNEVLAGVRHIRRTN VLLGAISLDLFAVLLGGAVALLPIFAKDVLEVGPTGLGLLRAAPALGAFIAALWL" BASE COUNT 1434 a 3390 c 3389 g 1722 t ORIGIN 1 ggctcggact gcacgtgctc cgccccggca tgccgttttt ggccagggca ctgcttcgcc 61 ggtcgcgcgc gtcggcgctc gttctccacc acgagctggt cggcgagggg ctcgtgcgct 121 atgcccagag gcgcggcgtt ccggtggtcg tctggacggt cgacgagccg cgtgacgtcg 181 agcgtcttga ccgtatagga gtcgatgcga tggtcgtgaa caatcccgtc atgttcacct 241 ctacactttc ggtgtgaaga gggggactct cttggtcgcc gcgggaatgc tcgcggtgtg 301 tgcgctgctc gggctcgcgt cgggattcgc gctagcgtcg actgacatcg cgccgtcgca 361 gacgaccgac cccccgccca cgacgtcgcc gccgccgacc gagacgagcc cgactccgcc 421 gcctgcgctg ccgccgaagc gccagctcat cgagccggga gtcatcgtcg gaggaatcgc 481 agtcggctgg ttgacgtcgc gaaccgcgag tgacctcgtg cgcaagacat tctcgcgacg 541 gctcgtcctg gtcgcaggcc caggccagcg ggtgcgtatc gcaccgcagg acctcggagc 601 cagcgcgagg atcgagaagg cgatccgacg ggcctcgagc gctcgaccgg gcgctcagat 661 tccgctcgag gtcgtggtgt cgcgctccaa gctcaagagc tacgtcgaga agctggccgc 721 gcgtttcgac cgcaaggcga tcgacgcggg agtcgtgctc tcggggatca ggccgcggtt 781 cgtcgaggcg caagacgggc ggcagctccg ggcaaaccgc accggcctcg cgctccggat 841 ctcgctcgcg actcacatgc gcgaccccat cccgctcgac ttcgacgtca cgaaacccga 901 ggtcagaacg acgaaggttg gctccgcgat tgtcatcctc cgcgactcga agcggctcct 961 cctctactcg acgaagaaga tggtccgccg atttggaatc gcgaccgggc agtcgtcgta 1021 tccgaccccg accggcgact acgagatcac gaccatgcag cgcaacccgt ggtggtatcc 1081 gccgccctcc gactgggcag cggaatccga ccccgttccg cctggtcccg gcaatccgct 1141 cggcacgcgc tggatgggga tctcggcgcc gtacgtcggg attcatggaa caccggacgc 1201 ggcctcgatc gggtactcgg cgtcgcacgg ctgcgtgcgc atgcgcattc ccgatgccga 1261 gtggctgttc cggcgggtgg aagtggggac gcccgtgttc atcctctccc gctaggtgct 1321 tcgcgacgca catacggtcg tgcgtcgacg acgagaaccg agcgggccta gcaagcaggc 1381 ccgagcacgc agggagggcc tgctcgccga tattggcccg actgaggacg acggcatgcg 1441 aagcgttcgc agtcgcgcga tgtgcggccg tctctgcgtc ggggagcacc acgcgtgagt 1501 cgccgcctgc tgctggtcgc gcagggtacg gctatcgggc tcgtcgtgct gctcttcgcg 1561 ctcctgctct ggaagctcgt tgccgacgag ggcggctcga tcgcatcggc gacggatcgc 1621 ggcgagcgcc cgccggctcc cgaattcacg ctcgagcgac tcgaccgcga aggcgacctg 1681 actctgtcct ccttgcgcgg gaaggcggtc gtcctcaact tctgggcctc gtggtgcatt 1741 ccgtgcaagg aggaggcgcc gtacctcgag gaggtgtggc aggcgaaccg cgacgacggt 1801 ctcgtcgtcg tcgggctcga tgccaaggac ttccgccgcg acgctcgggc gttcgcggag 1861 cgcttcgagc tcacgttccc gatcgtctac gacgggccgg gatcgacgct ctcgagctgg 1921 ggcgtgcagg gctttccgga gacgtttgtc atcgaccgcc gggaacgggt cgtcgaatcg 1981 ttcctcgggg cggtcaactc ggacgaggat cgcgcacgtc tacgcgcggc catcgagcgt 2041 gcgctcgagc cgtgaagcgc ttcgccccca cgctcgttct taccattgct ctcggactcc 2101 tcgtcccggt ctcggtctgg gcggctgacc cgccgagcgc ggccgatctc gaggcagagc 2161 tcgtctgccc cgtctgcgag acgaccctcg accagtcgaa cgctcctgtc gccgagcgca 2221 tgaaggtgtt catccgcgag cggatcgcgg cgggtgacac ggagcaagag atcaaggacg 2281 cgctcgtcgc ggagttcggg tccggcgtgc tcgcaacgcc gggcaaacgc gggttcgggc 2341 tgctcgcctg gttgataccg ctcgccgcga tcgcggtcgg ggcgctcgcc gtcggcctgc 2401 tcgtccgtgg ctggacaagg cggggggtgc cggccgacga tgatccggcc ccgctcgacg 2461 cgggcctcga gcggcggctc gacgaggagc tcgcgcgctt cgacggctga cgtggatccc 2521 ggctcagccc tcgccgcgtt cgtcgccggc ttcgtctcct tcctcgcgcc ctgcgtcctc 2581 ccgctcgtcc cgggctacct ctcggcgatc tccgtcctcg acgcgagcga gctcggccgg 2641 cccggacacg cgcgacgcgt catcgccgcc agcgtgccct tcgtcctcgg cttcacgatg 2701 ttcttcgtct cgctgggcgt tggggcggcg ctcgtcggcg gtcgcctgtt cagcgaccag 2761 tttctgctcg agaaggttgc gggcttcgtg ctcgtcgtct tcggactcgc gttcacgggg 2821 ctcctgccct ggccggagcg gctcgttggc gcgcggctgg tgcagggtgc gcgtggaagt 2881 ggttcccgac tattcctcgg cggagcgttc gcagtatgcg ccgccccctg catctcgcct 2941 gtcctcggcg ggatcctggt gctcgcgggc tcgtccggca cggtcgtcga gggcgcgctg 3001 ctgctggggg tctactcgct cgggctcgct gttccgttcg tactcgtcgg cgttctcttc 3061 accagggcaa tgggcgcttt ccgctggctg cgcgaccact accgcgtcat ccagttcgtc 3121 ggtggcgcga tcatggtcgc gctcggcctc ctgctcttct tcgagcgctt ctacgtcctc 3181 cgcatctacc tgaaccgttt cctcctgtgg ctcgggctcg agcccgcttt ctagacctcg 3241 actggatgta ggtcgaggtg accggcagtg acgtctcgct ctgccgcttc gacctgcgcg 3301 aggagcgagg cgacgtcgac gccgcgatgg aaaggtgcgt agggactcaa tcggcgacgc 3361 gccttctcga gttgccgctc gcaaccggga ccgtttcggc gctccgcgtg cagccatgcg 3421 accgcgacat gtacgaggcc ctggagaaag tcacgctcgg ctccgggtgc ctctcgccac 3481 tcgtcctcga gctcctcgtg cgcctcgaaa taggcgccct ttccgatcag ctcgagtccg 3541 gcgtcgagtc ccacggctcg attatcgaac gccttcggac tgctagtgtc gcggccgtgg 3601 cgacgatcct gctggctggg gtcgacctgt tcttccgggg caagctcgaa ggacttctgt 3661 ccgcgcacaa gctcgtgacg agcgacagcg tctcggcccc ggatctcgtg attgcggaca 3721 tcgcacgggt cgagccggag gaggtcgtcg aggcgtggcc ggacatcccg atcgtcggct 3781 tcacgaacca cacggaccgc gccggcctcc agcgtgcgca cgccgcgggc ttcgatcagg 3841 tgatcgtcaa atcggcgctc gtggagcgcg ccggggcgct cgtggacgag ctcatcggcg 3901 tggcctgacc aggctctcgt agccggccgg gagcggccaa gtgcctcggt agactcgtcc 3961 gcgtgacgta catcatcgcc gagccctgca tcgacatcaa agacctctcg tgcgtcgatg 4021 tgtgcccggt cgactgcatc cacgtgttcg agcggatcct catcatcgat ccggaggaat 4081 gcatcgactg cggtgcatgc gagccggagt gcccggtcga ggcgatcttt ccggaggacg 4141 cgctccccga caaatggaac gcgttcgtcg agatcaacta cgcgtttccc gacgccgcga 4201 agatcaaccc gctcgtggac aagtacgccg aggagaacga cgtccacaac gaaccgctcg 4261 agtagcagag gtcaggaagg caggagatag aggcagggcc tgacgtcgcc cggcagcggc 4321 gaagacgcaa ccttcgctac ccggtaacgg cgttccccgt tctcgacctc cgagccgacg 4381 agcggcacct cgccctcctg ctcgacgagc cggtaaccgg cgggcgacca tacgaagagg 4441 agatatccac ctgtgccgtt ggtgatcgcg gacacgatcc gatcgtagct accggcgcgc 4501 cgaggaggga ggaagctcca cgcgtatcga ggccccacga ggccagcgcg cctccgaagc 4561 gaagcggccg ttcagcgtcg ccgccctgtc cgccagcagc tcgagcaccg cgctgcggcg 4621 ctcgggaggc ccgtcgtccg cgatcaacag ctctgctccc ccgccttcga gcccgcgcag 4681 cccaacatcg atgcgcgtga gcgcgccgcg ccgcacggcc tgttcgaggg cgtcccgaac 4741 gatctgaaag agccccgtct gcgcgtgctc gccgaggctg tcgccgtgct cgacgtcaag 4801 gacgacctcg acgccacgac gggaggaaac gcgccgtccg agcgcctcga ccgctgacgc 4861 gaacccgagc gcgcggagcg cgcgaggctc gagatcctca cacagggcac gcaggtcctg 4921 ccccgcctcc cgtgtgagcg cgagtccacg ggtcaagagc tccaaggcgt cctgccggtc 4981 tccgtcccta agggccgcga gcgcggaatc gacgatctgc gtgatcgccg ccacatgctg 5041 caccgggccg tcgtggatca actcggcgag gcgccggcgt tcttcttgct cggccaggat 5101 ccgctcgccg acgagtgccc gctcggagtc ggagctcaat cgatcatcgc ctcgcgaagg 5161 gcgatggcaa ccgcctgcgt gcgcgtgtcg gcctcgagct tcgcaaggat gtggcggacg 5221 tggctcttga cggtctcctg gctgatgaag agcttcgccg cgacgtcgac gttcgacatg 5281 ccgtctgcga gcagttgcag gatctcgcgc tctcgcggcg ttaggacgtc cagctggccg 5341 cgactggcga tgaactcggc catgagcgcc gggtcgacga acgtgtctcc tgcggcaact 5401 ttctcgatgg cgcgaatgag cgtctcgtgg ggggtctcct tgaggatgta gccgcgcgcg 5461 cccgactcga gtccgcgttg catgagcgag cgctcgccgt aggcggtgaa gatgaggacg 5521 gcggtgcccg gcatcttctc gaggatctgc tccgtcgcct cgagcccgtc catgtccggc 5581 atgcggagat ccatgatcac cacgtccggg cgccggcgct cggtcagcgt gacggcggac 5641 tttccgtccg tcgcctcgcc gatcacgcga atgtgggggg agcgggagag cgccaaacgg 5701 agcccctcgc ggacgacctc atggtcgtcg gcgatcaggc aggtgatctc atcggccacg 5761 cggccagtct acgaggcggt ccggccggtt cctccctccc ggaaaggggg atatctcgtc 5821 acgcccaact agacaacaag gctgagctgt gcaggctcgc gttcacgtgc gcgctcggtc 5881 cgccggtagc gctccgaaag ctgcgtggcc cgagccgcca cgcgcacccg ctcgcgaatc 5941 ggggcggcga tcgcggacgg gaggtaagcg cgttccgcga acagctcctc gtaccgggtc 6001 acgtgctcgg gccagtcgcg cgcgagcgcc gccaggaagt gctcgcgcac cgcgggtctc 6061 agatggacgg gcgccgccca gacgcctcgt gctccagcgg cctttgccgc gtgcacgacg 6121 gcgtcgagct gctcccggcg gtcggagagg ccgggaagga tgggagcgag cccgaccccg 6181 acgtccactc ccgccgaggc gatgcgtcgc accgcctcga gccggctcga cggcggggcg 6241 gttcccggct ccgtcgtcct ccacacgcgc tcgtcgagcg tcggaatcga gaaggtgacg 6301 ctcacctcga ctcgcttcga ggcctcgagc agcacgtcga tgtcgcggac ggccagtggc 6361 ccccgtgtga tgatcgagaa gggagtccaa gccttctcga gctcgacgat gcatgcacgg 6421 gtgagccgaa accgcccttc cgcgggctgg tacgggtcgg tcgcggtgcc gatcgcgacc 6481 tcgtcgcgca cccaggaccg ccgggcgagc tcgcgcgcaa gtacgtcggc cacgttcgtt 6541 ttcactcgga tggatcgccc gtagcgctcg tcggaaggtc ggtcagcccg ccgctcgaag 6601 ttcggcacgt agcagaacgt gcagcgatgg acgcagccca tataggggtt gagcgaccag 6661 cggaaaggca tgccccggac cgggttgagc gccgatttgc acggctccat ccggtactcg 6721 acgcgcatgt ggtcagtata gaacgtatgt tcgtatgaga ccagcgctgt agcgttgccc 6781 gcatggccga gcccgagctt cccggcggaa tcaggcaggt cacccgtccg ctgccaacgc 6841 ggcccgggca cgtgcacgcg tacctgctgc ccggtgagga cgggtggacg atcgtcgaca 6901 ccggcatcgg tctcccggac gcgaaagaga gctggcgggc cgagctcgag cgggcaggag 6961 gacaggtggc gcgcatcttc gtgacgcact tccaccctga tcacgtcggt gcggcggcgg 7021 atctccacga gctgaccggc gcgcccgtct accaggggac gctcgactac gcacagtgcg 7081 agcttgtgtg ggggaacccg gcgtggcccg agcggctcat cgactggttt cgactgcacg 7141 ggaccccgga tgacgtgacc gaggagctcg tggggcagag ctccgtctac cgtccgtaca 7201 tccgctacca gccggatccg atcctcgtgc gcgcggggga gtggctcgat ggctgggagc 7261 tggtggcggc tgcggggcac gccgacgggc agctttgcct gctgaaggac ggcgtccttc 7321 tagcggcgga tcacgtgctc gagcggatct ctccgaccgt gggcctctgg cccgccagcc 7381 gtccggatcc gctcggcgac tatctggagg cgctcgaccg gacggtcaag atcgaaccgc 7441 gcatcgcgct gcccggtcac ggcgagctca tcgccgaccc ggccggtcgg gcgcgtgagc 7501 ttcaagagca tcatcggatc aggctccagg aagccgtggc tgcattcggc ccgaaaccgt 7561 tgagcggcta cgagctttcc ctcgtgctct ggggcgacgt tctgaagcct gctgcgcgac 7621 ggtttgcggt cgccgagtcg ctctcccatg cggagcggct tgtacgggtc ggcgcggccg 7681 tccgtcacga ggtcgacggg gctgtttcct atactgcggc ctgatcgtgg acgacgacga 7741 gctgccgcct agtaccggcg cccgcgcggg cttctctcta gtgggggcac tcctgccgac 7801 ttgagcacgt ccctgcgcat cgtcgtcatc ttcctactcg tgttcgcgaa cgcgatcttc 7861 gtcgcggccg aatacgcgct cgtgaccgcc cgccggagcc ggctcgagga gcgggcagtg 7921 aagggcgggc tcggggctcg aacggcgctt cgcctgatgg acgacccggt gcggttcatc 7981 agcaccgtgc agatcgggat cacggtctcc gcgatcctcc tcggcgctat cggcgagccg 8041 cttctctccg agctcatgga tccaccgctc tcgaacaccg tcgcgttcct gatcgcgttc 8101 gcgatcctga cgtacctctc cgttgtcgtc ggcgagctcg tgccgaaagc ggtcgcgctc 8161 cagaaggcgg agccgatcgc ggtcgcgctc gcggttccgc tcgactggat cgcgcgcttc 8221 gcgcacccgg tcgtctgggc gctccaggtc tcagcgaacg tcctcctcaa aggtctgcgg 8281 gtcaagcccg ccccaaccgg gatgatcgcc tacacgcgcg aggacatccg gtacagcgtc 8341 gcggctgccg aggacgtcgg cgagctgcac caggccgagg aagagatgct ctacaaggtc 8401 ttcgacttcg cgtcgaagga ggtctcggcg gtcatggtgc cgcggcccga ggtggttgcg 8461 atctcggtgg acgtgactcc cgaagaggcg ctggcgacga tcctcgactc gccgtacacg 8521 cgctacccgg tcttccggga gtcgctcgat cacatcgtcg gcgtcctaca cgtgcgcgac 8581 ctgttcgcgg ccatgcacga tcaagggatt gccgcggtcg agctggaagc gatcctcagg 8641 cccggatacg tcgtgccgga gacaaaggat ctcgccgcgc tccttgcgga cttcaggcgc 8701 aagaagcagc accaagcgat cgtcgtcgac gagtacggga cgatggcggg gatcgtgacg 8761 ctcgaggaca tcctcgagga gatcgtcggc gagatcgagg acgagttcga tctgcccgac 8821 gagtcggtcg agcgcgtcga cgagaagagg atccgcatcg acggcacgtt caccatcgac 8881 gacttcaacg agcagttcgc cacggagctc gagcaggacg actaccacac gatggcgggt 8941 ctcgtcttcg gcacgcttgg gcgccagcct gaggtcgggg actcggtcgc agtcaacggt 9001 cttcgcctcg aggtgctcga ggtcgacggc tcccgcatcg gacgtatcga gatcgagttc 9061 gacgccgaag agcagcctgc cggtgcgcca gaggccgcct agctccgtgc acctggcgtg 9121 agctcgggcg cgcgcgcggt cttcagccac cgcgacttca ggctctactg gatagcgata 9181 ttcgcggagg gcttcgggct cacgatggcg ctcgtcgcca tcggctggca ggtctactcc 9241 gttcgcggga acgcgctcga cctcggcctg gtggggctgg cggagtttct cccgctcctc 9301 ctgttcgcgc ttcccgccgg gcagctcgcc gaccgcgtct cgcgccggcg cctgctcgtc 9361 gtcatggtcg tgctggacat cgtgatgctc ggcgggctgc tcgtcgtcac cgtctcggga 9421 gcggacgagg tctggccctt cttcgcgctc gcgttcctct tcggcacgag cagcgccctc 9481 ggtgctccgg cgggccgtgc gctcacaccc tcgctcgtgc cggaggagtt gctcgtcagc 9541 gcgctcgcgc agcgctcgat cgtgaaccag atctcgctcg tgagcgggcc ggcggtcggc 9601 ggtctcctgt tcgccgtgcg cgctgagctc gtctatgcga tcgcaatcgc tctggcgctc 9661 gtctcgctgg tctgcttcct ctcgttgcgc acgggacgga tgcccgcggc ggaaggcgtc 9721 gaaggtctga acgaggtgct cgcaggcgtt cgccacatcc ggcgcacgaa cgtcctgctc 9781 ggcgcgatct ccctcgatct cttcgcggtg ctcctcggtg gcgcggttgc cctgctgccg 9841 atcttcgcga aggacgtgct cgaggtaggc ccgacggggc tcggcctctt gcgcgcggcc 9901 cccgcgctcg gcgcgttcat cgccgcgctc tggct //