LOCUS ECOMETH 4097 bp DNA linear BCT 20-OCT-1993 DEFINITION E.coli methionine synthase (metH) gene, complete cds. ACCESSION J04975 VERSION J04975.1 KEYWORDS metH gene; methionine synthase. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (bases 1 to 4097) AUTHORS Banerjee,R.V., Johnston,N.L., Sobeski,J.K., Datta,P. and Matthews,R.G. TITLE Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain JOURNAL J. Biol. Chem. 264 (23), 13888-13895 (1989) PUBMED 2668277 REFERENCE 2 (sites) AUTHORS Old,I.G., Margarita,D., Glass,R.E. and Saint Girons,I. TITLE Nucleotide sequence of the metH gene of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2 JOURNAL Gene 87 (1), 15-21 (1990) PUBMED 2185137 REFERENCE 3 (sites) AUTHORS Negre,D., Cortay,J.C., Old,I.G., Galinier,A., Richaud,C., Saint Girons,I. and Cozzone,A.J. TITLE Overproduction and characterization of the iclR gene product of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2 JOURNAL Gene 97 (1), 29-37 (1991) PUBMED 1995431 REFERENCE 4 (bases 1 to 4097) AUTHORS Drummond,J.T., Loo,R.R. and Matthews,R.G. TITLE Electrospray mass spectrometric analysis of the domains of a large enzyme: observation of the occupied cobalamin-binding domain and redefinition of the carboxyl terminus of methionine synthase JOURNAL Biochemistry 32 (36), 9282-9289 (1993) PUBMED 8369296 COMMENT On Jul 26, 1993 this sequence version replaced gi:146830. sequence for bases 3832-4097 taken from X16584 (Old et al.) Computer readable copy of sequence [1] kindly submitted by R.V.Banerjee, 07-JUL-1989. FEATURES Location/Qualifiers source 1..4097 /organism="Escherichia coli" /mol_type="unassigned DNA" /strain="K-12" /db_xref="taxon:562" gene 223..3906 /gene="metH" CDS 223..3906 /gene="metH" /EC_number="2.1.1.13" /note="metH promoter located within iclR gene, which is transcribed divergently from metH and encodes a repressor for the aceBAK operon" /citation=[3] /codon_start=1 /transl_table=11 /product="methionine synthase" /protein_id="AAA02995.1" /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE INFAAAKLARRCADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR DDGTERLLELAEKYRGTKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA RRKGMSVTEVERWLAPNLGYDAD" old_sequence 746 /gene="metH" /citation=[4] /replace="a" old_sequence 2144..2145 /gene="metH" /citation=[4] /replace="gc" old_sequence 2502 /gene="metH" /citation=[4] /replace="g" old_sequence 3332..3333 /gene="metH" /citation=[4] /replace="cg" old_sequence 3556..3558 /gene="metH" /citation=[4] /replace="tg" old_sequence 3805..3806 /gene="metH" /note="change results in extension of cds" /citation=[4] /replace="act" BASE COUNT 991 a 1052 c 1173 g 881 t ORIGIN 1 ttcgcgggaa tgggtgcgac catgacagtc tcttttttct gtatcgtgga aatcattttc 61 atttttattg ttagctaatg caatagttac tgaactgatc cgatgagtta atgttgaaca 121 aatctcatgt tgcgtggtgg tcgcttttac cacagatgcg tttatgccag tatggtttgt 181 tgaattttta ttaaatctgg gttgagcgtg tcgggagcaa gtgtgagcag caaagtggaa 241 caactgcgtg cgcagttaaa tgaacgtatt ctggtgctgg acggcggtat gggcaccatg 301 atccagagtt atcgactgaa cgaagccgat tttcgtggtg aacgctttgc cgactggcca 361 tgcgacctca aaggcaacaa cgacctgctg gtactcagta aaccggaagt gatcgccgct 421 atccacaacg cctactttga agcgggcgcg gatatcatcg aaaccaacac cttcaactcc 481 acgaccattg cgatggcgga ttaccagatg gaatccctgt cggcggaaat caactttgcg 541 gcggcgaaac tggcgcgacg ttgtgctgac gagtggaccg cgcgcacgcc agagaaaccg 601 cgctacgttg ccggtgttct cggcccgacc aaccgcacgg cgtctatttc tccggacgtc 661 aacgatccgg catttcgtaa tatcactttt gacgggctgg tggcggctta tcgagagtcc 721 accaaagcgc tggtggaagg tggcgcggat ctgatcctga ttgaaaccgt tttcgacacc 781 cttaacgcca aagcggcggt atttgcggtg aaaacggagt ttgaagcgct gggcgttgag 841 ctgccgatta tgatctccgg caccatcacc gacgcctccg ggcgcacgct ctccgggcag 901 accaccgaag cattttacaa ctcattgcgc cacgccgaag ctctgacctt tggcctgaac 961 tgtgcgctgg ggcccgatga actgcgccag tacgtgcagg agctgtcacg gattgcggaa 1021 tgctacgtca ccgcgcaccc gaacgccggg ctacccaacg cctttggtga gtacgatctc 1081 gacgccgaca cgatggcaaa acagatacgt gaatgggcgc aagcgggttt tctcaatatc 1141 gtcggcggct gctgtggcac cacgccacaa catattgcag cgatgagtcg tgcagtagaa 1201 ggattagcgc cgcgcaaact gccggaaatt cccgtagcct gccgtttgtc cggcctggag 1261 ccgctgaaca ttggcgaaga tagcctgttt gtgaacgtgg gtgaacgcac caacgtcacc 1321 ggttccgcta agttcaagcg cctgatcaaa gaagagaaat acagcgaggc gctggatgtc 1381 gcgcgtcaac aggtggaaaa cggcgcgcag attatcgata tcaacatgga tgaagggatg 1441 ctcgatgccg aagcggcgat ggtgcgtttt ctcaatctga ttgccggtga accggatatc 1501 gctcgcgtgc cgattatgat cgactcctca aaatgggacg tcattgaaaa aggtctgaag 1561 tgtatccagg gcaaaggcat tgttaactct atctcgatga aagagggcgt cgatgccttt 1621 atccatcacg cgaaattgtt gcgtcgctac ggtgcggcag tggtggtaat ggcctttgac 1681 gaacagggac aggccgatac tcgcgcacgg aaaatcgaga tttgccgtcg ggcgtacaaa 1741 atcctcaccg aagaggttgg cttcccgcca gaagatatca tcttcgaccc aaacatcttc 1801 gcggtcgcaa ctggcattga agagcacaac aactacgcgc aggactttat cggcgcgtgt 1861 gaagacatca aacgcgaact gccgcacgcg ctgatttccg gcggcgtatc taacgtttct 1921 ttctcgttcc gtggcaacga tccggtgcgc gaagccattc acgcagtgtt cctctactac 1981 gctattcgca atggcatgga tatggggatc gtcaacgccg ggcaactggc gatttacgac 2041 gacctacccg ctgaactgcg cgacgcggtg gaagatgtga ttcttaatcg tcgcgacgat 2101 ggcaccgagc gtttactgga gcttgccgag aaatatcgcg gcacgaaaac cgacgacacc 2161 gccaacgccc agcaggcgga gtggcgctcg tgggaagtga ataaacgtct ggaatactcg 2221 ctggtcaaag gcattaccga gtttatcgag caggataccg aagaagcccg ccagcaggct 2281 acgcgcccga ttgaagtgat tgaaggcccg ttgatggacg gcatgaatgt ggtcggcgac 2341 ctgtttggcg aagggaaaat gttcctgcca caggtggtca aatcggcgcg cgtcatgaaa 2401 caggcggtgg cctacctcga accgtttatt gaagccagca aagagcaggg caaaaccaac 2461 ggcaagatgg tgatcgccac cgtgaagggc gacgtccacg acatcggtaa aaatatcgtt 2521 ggtgtggtgc tgcaatgtaa caactacgaa attgtcgatc tcggcgttat ggtgcctgcg 2581 gaaaaaattc tccgtaccgc taaagaagtg aatgctgatc tgattggcct ttcggggctt 2641 atcacgccgt cgctggacga gatggttaac gtggcgaaag agatggagcg tcagggcttc 2701 actattccgt tactgattgg cggcgcgacg acctcaaaag cgcacacggc ggtgaaaatc 2761 gagcagaact acagcggccc gacggtgtat gtgcagaatg cctcgcgtac cgttggtgtg 2821 gtggcggcgc tgctttccga tacccagcgt gatgattttg tcgctcgtac ccgcaaggag 2881 tacgaaaccg tacgtattca gcacgggcgc aagaaaccgc gcacaccacc ggtcacgctg 2941 gaagcggcgc gcgataacga tttcgctttt gactggcagg cttacacgcc gccggtggcg 3001 caccgtctcg gcgtgcagga agtcgaagcc agcatcgaaa cgctgcgtaa ttacatcgac 3061 tggacaccgt tctttatgac ctggtcgctg gccgggaagt atccgcgcat tctggaagat 3121 gaagtggtgg gcgttgaggc gcagcggctg tttaaagacg ccaacgacat gctggataaa 3181 ttaagcgccg agaaaacgct gaatccgcgt ggcgtggtgg gcctgttccc ggcaaaccgt 3241 gtgggcgatg acattgaaat ctaccgtgac gaaacgcgta cccatgtgat caacgtcagc 3301 caccatctgc gtcaacagac cgaaaaaaca ggcttcgcta actactgtct cgctgacttc 3361 gttgcgccga agctttctgg taaagcagat tacatcggcg catttgccgt gactggcggg 3421 ctggaagagg acgcactggc tgatgccttt gaagcgcagc acgatgatta caacaaaatc 3481 atggtgaaag cgcttgccga ccgtttagcc gaagcctttg cggagtatct ccatgagcgt 3541 gtgcgtaaag tctactgggg ctatgcgccg aacgagaacc tcagcaacga agagctgatc 3601 cgcgaaaact accagggcat ccgtccggca ccgggctatc cggcctgccc ggaacatacg 3661 gaaaaagcca ccatctggga gctgctggaa gtggaaaaac acactggcat gaaactcaca 3721 gaatctttcg ccatgtggcc cggtgcatcg gtttcgggtt ggtacttcag ccacccggac 3781 agcaagtact acgctgtagc acaaattcag cgcgatcagg ttgaagatta tgcccgccgt 3841 aaaggtatga gcgttaccga agttgagcgc tggctggcac cgaatctggg gtatgacgcg 3901 gactgattca caaatctgtc acttttcctt acaacaaaca gggcgctcaa tgagtgcctg 3961 tgtgtttatt aatatgaaac acttatagtg gaaacaggct ggaataatct tagccggaaa 4021 aacgaatgtc aaaaggcaca gtaaacatgc cagtcagcgt gtttccggtc ctttggtcat 4081 tcaattgctt caggaac //