LOCUS PLY2CG 5297 bp DNA circular VRL 02-MAY-2003 DEFINITION Murine polyomavirus strain A2, complete genome. ACCESSION J02288 J02290 J02291 J02292 K00932 K00997 K01041 K01071 K01072 V01117 VERSION J02288.1 KEYWORDS T-antigen; antigen; capsid protein; circular; coat protein; complete genome; core region; large T-antigen; major structural protein VP1; middle T-antigen; minor structural protein VP2; minor structural protein VP3; origin of replication; small T-antigen. SOURCE Murine polyomavirus strain A2 ORGANISM Murine polyomavirus strain A2 Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus; Alphapolyomavirus muris. REFERENCE 9 (sites) AUTHORS Flavell,A.J., Cowie,A., Legon,S. and Kamen,R. TITLE Multiple 5' terminal cap structures in late polyoma virus RNA JOURNAL Cell 16 (2), 357-371 (1979) PUBMED 222458 REFERENCE 10 (sites) AUTHORS Legon,S., Flavell,A.J., Cowie,A. and Kamen,R. TITLE Amplification in the leader sequence of late polyoma virus mRNAs JOURNAL Cell 16 (2), 373-388 (1979) PUBMED 222459 REFERENCE 11 (bases 401 to 842) AUTHORS Hattori,J., Carmichael,G.G. and Benjamin,T.L. TITLE DNA sequence alterations in Hr-t deletion mutants of polyoma virus JOURNAL Cell 16 (3), 505-513 (1979) PUBMED 222464 REFERENCE 12 (bases 1 to 1567; 5267 to 5297) AUTHORS Soeda,E., Arrand,J.R., Smolar,N. and Griffin,B.E. TITLE Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens JOURNAL Cell 17 (2), 357-370 (1979) PUBMED 222480 REFERENCE 13 (sites) AUTHORS Friedmann,T., Esty,A., LaPorte,P. and Deininger,P. TITLE The nucleotide sequence and genome organization of the polyoma early region: extensive nucleotide and amino acid homology with SV40 JOURNAL Cell 17 (3), 715-724 (1979) PUBMED 225042 REFERENCE 14 (sites) AUTHORS Deininger,P., Esty,A., LaPorte,P. and Friedmann,T. TITLE Nucleotide sequence and genetic organization of the polyoma late region: features common to the polyoma early region and SV40 JOURNAL Cell 18 (3), 771-779 (1979) PUBMED 229965 REFERENCE 15 (sites) AUTHORS Manor,H., Wu,M., Baran,N. and Davidson,N. TITLE Electron microscopic mapping of RNA transcribed from the late region of polyoma virus DNA JOURNAL J. Virol. 32 (1), 293-303 (1979) PUBMED 232179 REFERENCE 16 (sites) AUTHORS Wells,R.D., Hutchinson,M.A. and Eckhart,W. TITLE Isolation and characterization of polyoma virus genomes with deletions between the origin of viral DNA replication and the site of initiation of translation in the early region JOURNAL J. Virol. 32 (2), 517-522 (1979) PUBMED 228074 REFERENCE 17 (sites) AUTHORS Bendig,M.M. and Folk,W.R. TITLE Deletion mutants of polyoma virus defining a nonessential region between the origin of replication and the initiation codon for early proteins JOURNAL J. Virol. 32 (2), 530-535 (1979) PUBMED 228076 REFERENCE 18 (bases 175 to 2922) AUTHORS Soeda,E., Arrand,J.R. and Griffin,B.E. TITLE Polyoma virus. The early region and its T-antigens JOURNAL Nucleic Acids Res. 7 (4), 839-857 (1979) PUBMED 228249 REFERENCE 19 (sites) AUTHORS Hunter,T., Hutchinson,M.A., Eckhart,W., Friedmann,T., Esty,A., LaPorte,P. and Deininger,P. TITLE Regions of the polyoma genome coding for T antigens JOURNAL Nucleic Acids Res. 7 (8), 2275-2288 (1979) PUBMED 230465 REFERENCE 20 (sites) AUTHORS Katinka,M., Yaniv,M., Vasseur,M. and Blangy,D. TITLE Expression of polyoma early functions in mouse embryonal carcinoma cells depends on sequence rearrangements in the beginning of the late region JOURNAL Cell 20 (2), 393-399 (1980) PUBMED 6248241 REFERENCE 21 (sites) AUTHORS Bendig,M.M., Thomas,T. and Folk,W.R. TITLE Regulatory mutants of polyoma virus defective in DNA replication and the synthesis of early proteins JOURNAL Cell 20 (2), 401-409 (1980) PUBMED 6248242 REFERENCE 22 (sites) AUTHORS Kamen,R., Favaloro,J., Parker,J., Treisman,R., Lania,L., Fried,M. and Mellor,A. TITLE Comparison of polyoma virus transcription in productively infected mouse cells and transformed rodent cell lines JOURNAL Cold Spring Harb. Symp. Quant. Biol. 44 (PT 1), 63-75 (1980) PUBMED 6253165 REFERENCE 23 (sites) AUTHORS Hunter,T., Hutchinson,M.A., Eckhart,W., Friedmann,T., Esty,A., La Porte,P. and Deininger,P. TITLE Organization of T antigens in the polyoma virus genome JOURNAL Cold Spring Harb. Symp. Quant. Biol. 44 (PT 1), 131-139 (1980) PUBMED 6253126 REFERENCE 24 (sites) AUTHORS Benjamin,T.L., Carmichael,G.G. and Schaffhausen,B.S. TITLE The hr-t gene of polyoma virus JOURNAL Cold Spring Harb. Symp. Quant. Biol. 44 (PT 1), 263-270 (1980) PUBMED 6253140 REFERENCE 25 (sites) AUTHORS Griffin,B.E., Ito,Y., Novak,U., Spurr,N., Dilworth,S., Smolar,N., Pollack,R., Smith,K. and Rifkin,D.B. TITLE Early mutants of polyoma virus (dl8 and dl23) with altered transformation properties: is polyoma virus middle T antigen a transforming gene product? JOURNAL Cold Spring Harb. Symp. Quant. Biol. 44 (PT 1), 271-283 (1980) PUBMED 6253141 REFERENCE 26 (bases 3946 to 5297) AUTHORS Arrand,J.R., Soeda,E., Walsh,J.E., Smolar,N. and Griffin,B.E. TITLE Polyoma virus DNA: Sequence from the late region that specifies the leader sequence for late mRNA and codes for VP2, VP3, and the N-terminus of VP1 JOURNAL J. Virol. 33 (2), 606-618 (1980) PUBMED 6251236 REFERENCE 27 (bases 2896 to 4160) AUTHORS Soeda,E., Arrand,J.R. and Griffin,B.E. TITLE Polyoma virus DNA: complete nucleotide sequence of the gene which codes for polyoma virus capsid protein VP1 and overlaps the VP2/VP3 genes JOURNAL J. Virol. 33 (2), 619-630 (1980) PUBMED 6251237 REFERENCE 28 (sites) AUTHORS Kamen,R., Favaloro,J. and Parker,J. TITLE Topography of the three late mRNA's of polyoma virus which encode the virion proteins JOURNAL J. Virol. 33 (2), 637-651 (1980) PUBMED 6251239 REFERENCE 29 (sites) AUTHORS Flavell,A.J., Cowie,A., Arrand,J.R. and Kamen,R. TITLE Localization of three major cappe 5' ends of polyoma virus late mRNA's within a single tetranucleotide sequence in the viral genome JOURNAL J. Virol. 33 (2), 902-908 (1980) PUBMED 6251254 REFERENCE 30 (bases 1070 to 1180) AUTHORS Bendig,M.M., Thomas,T. and Folk,W.R. TITLE Viable deletion mutant in the medium and large T-antigen-coding sequences of the polyoma virus genome JOURNAL J. Virol. 33 (3), 1215-1220 (1980) PUBMED 6245272 REFERENCE 31 (sites) AUTHORS Soeda,E., Arrand,J.R., Smolar,N., Walsh,J.E. and Griffin,B.E. TITLE Coding potential and regulatory signals of the polyoma virus genome JOURNAL Nature 283 (5746), 445-453 (1980) PUBMED 6243401 REFERENCE 32 (sites) AUTHORS Deninger,P.L., Esty,A., LaPorte,P., Hsu,H. and Friedmann,T. TITLE The nucleotide sequence and restriction enzyme sites of the polyoma genome JOURNAL Nucleic Acids Res. 8 (4), 855-860 (1980) PUBMED 6253927 REFERENCE 33 (sites) AUTHORS Treisman,R. TITLE Characterisation of polyoma late mRNA leader sequences by molecular cloning and DNA sequence analysis JOURNAL Nucleic Acids Res. 8 (21), 4867-4888 (1980) PUBMED 6255435 REFERENCE 34 (sites) AUTHORS Fujimura,F.K., Deininger,P.L., Friedmann,T. and Linney,E. TITLE Mutation near the polyoma DNA replication origin permits productive infection of F9 embryonal carcinoma cells JOURNAL Cell 23 (3), 809-814 (1981) PUBMED 6261957 REFERENCE 35 (sites) AUTHORS Herbomel,P., Saragosti,S., Blangy,D. and Yaniv,M. TITLE Fine structure of the origin-proximal DNAase I-hypersensitive region in wild-type and EC mutant polyoma JOURNAL Cell 25 (3), 651-658 (1981) PUBMED 6269751 REFERENCE 36 (bases 1 to 5297) AUTHORS Griffin,B.E., Soeda,E., Barrell,B.G. and Staden,R. TITLE Appendix B: Sequence and analysis of polyoma virus DNA JOURNAL (in) Tooze,J. (Ed.); DNA TUMOR VIRUSES - SECOND EDITION REVISED: 843-910; Cold Spring Harbor Laboratory, Cold Spring Harbor (1981) REFERENCE 37 (sites) AUTHORS Treisman,R., Cowie,A., Favaloro,J., Jat,P. and Kamen,R. TITLE The structures of the spliced mRNAs encoding polyoma virus early region proteins JOURNAL J. Mol. Appl. Genet. 1 (2), 83-92 (1981) PUBMED 6286820 REFERENCE 38 (sites) AUTHORS Srivatsan,E.S., Deininger,P.L. and Friedmann,T. TITLE Nucleotide sequence at polyoma VP1 mRNA splice sites JOURNAL J. Virol. 37 (1), 244-247 (1981) PUBMED 6260979 REFERENCE 39 (sites) AUTHORS Deininger,P.L., LaPorte,P. and Friedmann,T. TITLE Nucleotide sequence changes in polyoma ts-a mutants: correlation with protein structure JOURNAL J. Virol. 37 (3), 871-875 (1981) PUBMED 6262533 REFERENCE 40 (sites) AUTHORS Thomas,T., Vollmer,P. and Folk,W.R. TITLE Nucleotide sequence changes in polyoma virus A gene mutants JOURNAL J. Virol. 37 (3), 1094-1098 (1981) PUBMED 6262530 REFERENCE 41 (sites) AUTHORS Smolar,N. and Griffin,B.E. TITLE DNA sequences of polyoma virus early deletion mutants JOURNAL J. Virol. 38 (3), 958-967 (1981) PUBMED 6264166 REFERENCE 42 (sites) AUTHORS Magnusson,G., Nilsson,M.G., Dilworth,S.M. and Smolar,N. TITLE Characterization of polyoma mutants with altered middle and large T-antigens JOURNAL J. Virol. 39 (3), 673-683 (1981) PUBMED 6270343 REFERENCE 43 (sites) AUTHORS Katinka,M., Vasseur,M., Montreau,N., Yaniv,M. and Blangy,D. TITLE Polyoma DNA sequences involved in control of viral gene expression in murine embryonal carcinoma cells JOURNAL Nature 290 (5808), 720-722 (1981) PUBMED 6261151 REFERENCE 44 (sites) AUTHORS Treisman,R., Novak,U., Favaloro,J. and Kamen,R. TITLE Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein JOURNAL Nature 292 (5824), 595-600 (1981) PUBMED 6265803 REFERENCE 45 (bases 1416 to 1502) AUTHORS Novak,U. and Griffin,B.E. TITLE Requirement for the C-terminal region of middle T-antigen in cellular transformation by polyoma virus JOURNAL Nucleic Acids Res. 9 (9), 2055-2073 (1981) PUBMED 6272223 REFERENCE 46 (sites) AUTHORS Gaudray,P., Tyndall,C., Kamen,R. and Cuzin,F. TITLE The high affinity binding site on polyoma virus DNA for the viral large-T protein JOURNAL Nucleic Acids Res. 9 (21), 5697-5710 (1981) PUBMED 6273805 REFERENCE 47 (bases 5003 to 5297) AUTHORS Tyndall,C., La Mantia,G., Thacker,C.M., Favaloro,J. and Kamen,R. TITLE A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication JOURNAL Nucleic Acids Res. 9 (23), 6231-6250 (1981) PUBMED 6275353 REFERENCE 48 (sites) AUTHORS Cowie,A., Tyndall,C. and Kamen,R. TITLE Sequences at the capped 5'-ends of polyoma virus late region mRNAs: an example of extreme terminal heterogeneity JOURNAL Nucleic Acids Res. 9 (23), 6305-6322 (1981) PUBMED 6275357 REFERENCE 49 (bases 5218 to 5252) AUTHORS Sekikawa,K. and Levine,A.J. TITLE Isolation and characterization of polyoma host range mutants that replicate in nullipotential embryonal carcinoma cells JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78 (2), 1100-1104 (1981) PUBMED 6262755 REFERENCE 50 (sites) AUTHORS Eckhart,W., Delbruck,S., Deininger,P., Friedmann,T. and Hunter,T. TITLE A mutation increasing the size of the polyoma virion proteins, VP2 and VP3 JOURNAL Virology 109 (1), 35-46 (1981) PUBMED 6258312 REFERENCE 51 (sites) AUTHORS Tanaka,K., Chowdhury,K., Chang,K.S., Israel,M. and Ito,Y. TITLE Isolation and characterization of polyoma virus mutants which grow in murine embryonal carcinoma and trophoblast cells JOURNAL EMBO J. 1 (12), 1521-1527 (1982) PUBMED 6327274 REFERENCE 52 (sites) AUTHORS Kamen,R., Jat,P., Treisman,R., Favaloro,J. and Folk,W.R. TITLE 5' termini of polyoma virus early region transcripts synthesized in vivo by wild-type virus and viable deletion mutants JOURNAL J. Mol. Biol. 159 (2), 189-224 (1982) PUBMED 6292432 REFERENCE 53 (sites) AUTHORS Cowie,A., Jat,P. and Kamen,R. TITLE Determination of sequences at the capped 5' ends of polyoma virus early region transcripts synthesized in vivo and in vitro demonstrates an unusual microheterogeneity JOURNAL J. Mol. Biol. 159 (2), 225-255 (1982) PUBMED 6292433 REFERENCE 54 (bases 1 to 102; 5098 to 5297) AUTHORS Luthman,H., Nilsson,M.G. and Magnusson,G. TITLE Non-contiguous segments of the polyoma genome required in cis for DNA replication JOURNAL J. Mol. Biol. 161 (4), 533-550 (1982) PUBMED 6296399 REFERENCE 55 (sites) AUTHORS Templeton,D. and Eckhart,W. TITLE Mutation causing premature termination of the polyoma virus medium T antigen blocks cell transformation JOURNAL J. Virol. 41 (3), 1014-1024 (1982) PUBMED 6284958 REFERENCE 56 (sites) AUTHORS Vasseur,M., Katinka,M., Herbomel,P., Yaniv,M. and Blangy,D. TITLE Physical and biological features of polyoma virus mutants able to infect embryonal carcinoma cell lines JOURNAL J. Virol. 43 (3), 800-808 (1982) PUBMED 6292462 REFERENCE 57 (sites) AUTHORS Gelinas,C., Chartrand,P. and Bastin,M. TITLE Polyoma virus mutant with normal transforming ability but impaired tumorigenic potential JOURNAL J. Virol. 43 (3), 1072-1081 (1982) PUBMED 6292450 REFERENCE 58 (sites) AUTHORS Ding,D., Dilworth,S.M. and Griffin,B.E. TITLE mlt Mutants of polyoma virus JOURNAL J. Virol. 44 (3), 1080-1083 (1982) PUBMED 6294323 REFERENCE 59 (sites) AUTHORS Jat,P., Novak,U., Cowie,A., Tyndall,C. and Kamen,R. TITLE DNA sequences required for specific and efficient initiation of transcription at the polyoma virus early promoter JOURNAL Mol. Cell. Biol. 2 (7), 737-751 (1982) PUBMED 6100910 REFERENCE 60 (sites) AUTHORS Katinka,M. and Yaniv,M. TITLE Deletions of N-terminal sequences of polyoma virus T-antigens reduce but do not abolish transformation of rat fibroblasts JOURNAL Mol. Cell. Biol. 2 (10), 1238-1246 (1982) PUBMED 6294500 REFERENCE 61 (sites) AUTHORS Jat,P., Roberts,J.W., Cowie,A. and Kamen,R. TITLE Comparison of the polyoma virus early and late promoters by transcription in vitro JOURNAL Nucleic Acids Res. 10 (3), 871-887 (1982) PUBMED 6174941 REFERENCE 62 (sites) AUTHORS Nilsson,S.V. and Magnusson,G. TITLE T-antigen expression by polyoma mutants with modified RNA splicing JOURNAL EMBO J. 2 (12), 2095-2101 (1983) PUBMED 6321148 REFERENCE 63 (sites) AUTHORS Hayday,A.C., Chaudry,F. and Fried,M. TITLE Loss of polyoma virus infectivity as a result of a single amino acid change in a region of polyoma virus large T-antigen which has extensive amino acid homology with simian virus 40 large T-antigen JOURNAL J. Virol. 45 (2), 693-699 (1983) PUBMED 6300429 REFERENCE 64 (bases 1113 to 1249) AUTHORS Nilsson,S.V., Tyndall,C. and Magnusson,G. TITLE Deletion mapping of a short polyoma virus middle T antigen segment important for transformation JOURNAL J. Virol. 46 (1), 284-287 (1983) PUBMED 6298462 REFERENCE 65 (bases 1 to 163; 5267 to 5297) AUTHORS Katinka,M. and Yaniv,M. TITLE DNA replication origin of polyoma virus: early proximal boundary JOURNAL J. Virol. 47 (1), 244-248 (1983) PUBMED 6306277 REFERENCE 66 (sites) AUTHORS Muller,W.J., Mueller,C.R., Mes,A.M. and Hassell,J.A. TITLE Polyomavirus origin for DNA replication comprises multiple genetic elements JOURNAL J. Virol. 47 (3), 586-599 (1983) PUBMED 6312083 REFERENCE 67 (sites) AUTHORS Pomerantz,B.J., Mueller,C.R. and Hassell,J.A. TITLE Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome JOURNAL J. Virol. 47 (3), 600-610 (1983) PUBMED 6312084 REFERENCE 68 (bases 1123 to 1200) AUTHORS Oostra,B.A., Harvey,R., Ely,B.K., Markham,A.F. and Smith,A.E. TITLE Transforming activity of polyoma virus middle-T antigen probed by site-directed mutagenesis JOURNAL Nature 304 (5925), 456-459 (1983) PUBMED 6308461 REFERENCE 69 (sites) AUTHORS Ito,Y. and Griffen,B.E. TITLE Genetic map of polyoma virus JOURNAL (in) O'Brien,S.J. (Ed.); GENETIC MAPS, Vol. 3: 66-76; Cold Spring Harbor Laboratory, Cold Spring Harbor (1984) REFERENCE 70 (sites) AUTHORS Pomerantz,B.J. and Hassell,J.A. TITLE Polyomavirus and simian virus 40 large T antigens bind to common DNA sequences JOURNAL J. Virol. 49 (3), 925-937 (1984) PUBMED 6321773 REFERENCE 71 (bases 93 to 183) AUTHORS Zhu,Z.Y., Veldman,G.M., Cowie,A., Carr,A., Schaffhausen,B. and Kamen,R. TITLE Construction and functional characterization of polyomavirus genomes that separately encode the three early proteins JOURNAL J. Virol. 51 (1), 170-180 (1984) PUBMED 6328036 REFERENCE 72 (bases 1018 to 1663) AUTHORS Gelinas,C., Masse,S. and Bastin,M. TITLE mlt Mutation in the polyomavirus genome impairing a function of the middle T protein JOURNAL J. Virol. 51 (1), 242-246 (1984) PUBMED 6328044 REFERENCE 73 (sites) AUTHORS Triezenberg,S.J. and Folk,W.R. TITLE Essential nucleotides in the polyomavirus origin region JOURNAL J. Virol. 51 (2), 437-444 (1984) PUBMED 6086955 REFERENCE 74 (bases 1 to 202; 5202 to 5297) AUTHORS Cowie,A. and Kamen,R. TITLE Multiple binding sites for polyomavirus large T antigen within regulatory sequences of polyomavirus DNA JOURNAL J. Virol. 52 (3), 750-760 (1984) PUBMED 6092707 REFERENCE 75 (sites) AUTHORS Templeton,D. and Eckhart,W. TITLE N-terminal amino acid sequences of the polyoma middle-size T antigen are important for protein kinase activity and cell transformation JOURNAL Mol. Cell. Biol. 4 (5), 817-821 (1984) PUBMED 6328268 REFERENCE 76 (bases 1 to 202; 5253 to 5297) AUTHORS Dilworth,S.M., Cowie,A., Kamen,R.I. and Griffin,B.E. TITLE DNA binding activity of polyoma virus large tumor antigen JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81 (7), 1941-1945 (1984) PUBMED 6326093 REFERENCE 77 (sites) AUTHORS Melin,F., Pinon,H., Kress,C. and Blangy,D. TITLE Isolation of polyomavirus mutants multiadapted to murine embryonal carcinoma cells JOURNAL J. Virol. 53 (3), 862-866 (1985) PUBMED 2983095 REFERENCE 79 (sites) AUTHORS Veldman,G.M., Lupton,S. and Kamen,R. TITLE Polyomavirus enhancer contains multiple redundant sequence elements that activate both DNA replication and gene expression JOURNAL Mol. Cell. Biol. 5 (4), 649-658 (1985) PUBMED 2985964 REFERENCE 80 (bases 1 to 5297) AUTHORS De Simone,V., La Mantia,G., Lania,L. and Amati,P. TITLE Polyomavirus mutation that confers a cell-specific cis advantage for viral DNA replication JOURNAL Mol. Cell. Biol. 5 (8), 2142-2146 (1985) PUBMED 3018552 REFERENCE 81 (bases 1 to 5297) AUTHORS Griffin,B.E., Fried,M. and Cowie,A. TITLE Polyoma DNA: a physical map JOURNAL Proc. Natl. Acad. Sci. U.S.A. 71 (5), 2077-2081 (1974) PUBMED 4365585 REFERENCE 82 (bases 1 to 5297) AUTHORS Kamen,R., Sedat,J. and Ziff,E. TITLE Orientation of the complementary strands of polyoma virus DNA with respect to the DNA physical map JOURNAL J. Virol. 17 (1), 212-218 (1975) PUBMED 173885 REFERENCE 83 (bases 1 to 80) AUTHORS Soeda,E., Miura,K., Nakaso,A. and Kimura,G. TITLE Nucleotide sequence around the replication origin of polyoma virus DNA JOURNAL FEBS Lett. 79 (2), 383-389 (1977) PUBMED 196918 REFERENCE 84 (bases 1 to 80) AUTHORS Soeda,E., Kimura,G. and Miura,K. TITLE Similarity of nucleotide sequences around the origin of DNA replication in mouse polyoma virus and simian virus 40 JOURNAL Proc. Natl. Acad. Sci. U.S.A. 75 (1), 162-166 (1978) PUBMED 203928 REFERENCE 85 (bases 1 to 5297) AUTHORS Friedmann,T., Doolittle,R.F. and Walter,G. TITLE Amino acid sequence homology between polyoma and SV40 tumour antigens deduced from nucleotide sequences JOURNAL Nature 274 (5668), 291-293 (1978) PUBMED 210390 REFERENCE 86 (bases 1 to 5297) AUTHORS Friedmann,T., LaPorte,P. and Esty,A. TITLE Nucleotide sequence studies of polyoma DNA. The Hpa II 3/5 junction to the Hpa II 4/Hae III 18 junction, encoding the origin of DNA replication and the 5' end of the early region JOURNAL J. Biol. Chem. 253 (18), 6561-6567 (1978) PUBMED 210189 REFERENCE 87 (bases 438 to 810) AUTHORS Soeda,E. and Griffin,B.E. TITLE Sequences from the genome of a non-transforming mutant of polyoma virus JOURNAL Nature 276 (5685), 294-298 (1978) PUBMED 213731 REFERENCE 88 (bases 1 to 5297) AUTHORS Horowitz,M., Bratosin,S. and Aloni,Y. TITLE Polyoma infected cells contain at least three spliced late RNAs JOURNAL Nucleic Acids Res. 5 (12), 4663-4675 (1978) PUBMED 218167 REFERENCE 89 (bases 1 to 5297) AUTHORS Scheller,A. and Prives,C. TITLE Simian virus 40 and polyomavirus large tumor antigens have different requirements for high-affinity sequence-specific DNA binding JOURNAL J. Virol. 54 (2), 532-545 (1985) PUBMED 2985816 COMMENT [1] sites; physical map of the genome. [2] sites; polarity of e and l strands. [3] approximate, revised by [12]. [7] sites; 3' ends of late mRNA leaders. [4] sites; bases 1 to 783; 5191 to 5297 of a3; see separate entry. [5] sites; bases 175 to 783 of a3; see separate entry. [6] a2 and mutant ng-18. [8] approximate, revised by [12]. [15] sites; late mRNA transcripts. [16] sites; deletion mutants in noncoding region. [17] sites; mutant analysis of replication origin boundaries. [9] sites; late mRNA 5' terminal cap structures. [10] sites; late mRNA leader sequence amplification. [11] wt and mutants. [12] revised by [18]. [13] sites; bases 1 to 2993; 5273 to 5297 of a3; see separate entry. [19] sites; bases 169 to 1547 of a3; see separate entry. [22] sites; early mRNA multiple start sites. [23] sites; t antigen cds initiation and splicing. [24] sites; ts-a and hr-t mutants. [25] sites; deletion mutants in middle t-antigen cds. [14] sites; bases 2910 to 5272 of a3; see separate entry. [32] sites; bases 5273 to 5297 of a3; see separate entry. [31] review; bases 1 to 5297. [20] sites; mutants py204, py97. [21] sites; mutants in origin of replication and early region. [27] comp strand. [28] sites; late mRNA topography. [29] sites; major cap sites of late mRNAs. [30] wt and mutants. [33] sites; late mRNA splice sites and leader sequences. [37] sites; late mRNA splice sites and leader sequences. [45] wt and mutants. [46] sites; large t-antigen binding sites. [48] sites; cap sites for late mRNAs. [49] wt and mutants. [43] sites; replication origin mutants. [(in) Tooze,J. (Eds.);DNA Tumor Viruses - Second Edition Revised: 843-910;Cold Sp] review; bases 1 to 5297. [44] sites; ppymt1 mutation. [38] sites; VP1 mRNA splice sites. [34] sites; bases 5178 to 5272 of a3 wt and mutants. [35] sites; DNAase I hypersensitive map. [50] sites; bases 3976 to 4092 of a3 wt and mutants. [39] sites; ts-a mutant analysis. [40] sites; analysis of ts-a and ts-25e mutants. [41] sites; t antigen cds mutants. [42] sites; t antigen cds mutants. [52] sites; 5' termini of early mRNAs. [53] sites; 5' termini of early mRNAs. [54] wt and mutants. [59] sites; early mRNA promoter sequences. [60] sites; N-terminal deletion studies of t-antigens. [61] sites; early mRNA promoter sequences. [55] sites; mutants in a3 middle t-antigen cds. [56] sites; mutants in noncoding region. [57] sites; mutants in a3 t antigen cds. [58] sites; analysis of t-antigen deletion mutants. [51] sites; bases 5093 to 5193 of a3; see separate entry. [62] sites; t-antigen expression in mutants. [63] sites; mutants in large t-antigen cds. [64] wt and mutants. [66] sites; replication origin elements. [67] sites; large t-antigen binding sites. [75] sites; mutants in t-antigen cds. [70] sites; large t-antigen binding sites. [71] wt and recombinants. [72] wt and mutants. [73] sites; bases 1 to 77; 5273 to 5297 of a3; see separate entry. [(in) O'Brien,S.J. (ed.);Genetic Maps, Volume 3: 66-76;Cold Spring Harbor Laborat] review; bases 1 to 5297. [77] sites; mutants in enhancer and origin of replication regions. [78] sites; large-t antigen binding sites. [79] sites; enhancer sequence. [80] sites; PyFL78 mutant; see separate entry. Kindly being reviewed by Dr. G. Veldman, Genetics Institute, Cambridge, MA. 02140. The l strand of the polyoma a2 strain (with the polarity of the early mRNA) is shown below, as reported by [31] and [(in) Tooze,J. (Eds.);DNA Tumor Viruses - Second Edition Revised: 843-910;Cold Sp], with the corrections of [47] and [60]. The latter corrections of the sequence bring the a2 and a3 sequences into closer agreement, especially throughout the noncoding (origin of replication) region. Nevertheless, for convenience, the a3 sequence has been reported as a separate entry, . For comparison, align base 1 below with base 26 of , or alternatively, base 5273 below with base 1 of . The map units for the two sequences become virtually the same when, by analogy with SV40, the middle base of the single EcoRI site is taken to be the origin. Hence for the sequence below, pya2 + 3733 mu = --------------- x 100. 5297 Both a2 and a3 sequence studies are included in this entry insofar as they define features and sites of 'wild-type' polyoma (which may differ slightly from either laboratory strain). The principal sources of the annotation herein are [31], [(in) Tooze,J. (Eds.);DNA Tumor Viruses - Second Edition Revised: 843-910;Cold Sp] and [(in) O'Brien,S.J. (ed.);Genetic Maps, Volume 3: 66-76;Cold Spring Harbor Laborat]. The start sites for the mRNAs, both early and late, are only awkwardly annotated in the sites table below given the conspicuous microheterogeneity of their cap sites (see, for instance, figures B.5 and B.6 of [(in) Tooze,J. (Eds.);DNA Tumor Viruses - Second Edition Revised: 843-910;Cold Sp]). For the early mRNAs, potential promoter elements are found at bases 5080, 5153, 122 and 274. For the late mRNAs, a potential promoter element is found at base 5157 on the comp strand. Late mRNAs vary in length for reasons other than cap site heterogeneity: the leaders for the 19S, 18S and 16S mRNAs (mVP2, mVP3 and mVP1 respectively) display differential splicing, and each can vary depending upon the number of tandem repeats embodied from bases 5021 to 5078 of the comp strand. Between six and none of these repeats can be found in a leader. DNA replication, as with SV40, is bidirectional from a region overlapping the mRNA initiation regions; bases 5044-5297 and 1-92 approximately. This origin of replication is considered to comprise an alpha element, a beta element (one of which, but not both, is required) and a core. An 'AT'-rich region (bases 5272-5288) and a 32 bp 'GC'-rich palindrome (bases 5286-5297; 1 to 22) are major constituents of the region. The large t-antigen is required for viral replication. Data pertinent to the binding sites differ (two sites or three) but all agree that the pentanucleotide 'GAGGC' is involved. Both similarities and differences have been argued for the large t-antigen binding of polyoma and SV40 [70],[74]. The middle t-antigen, not found to date in SV40, is essential for cell transformation by polyoma. Mutants (to be annotated in a future update; see table 4 of [(in) O'Brien,S.J. (ed.);Genetic Maps, Volume 3: 66-76;Cold Spring Harbor Laborat]) affecting small and middle t-antigen are denoted hr-t, for host-range transformation, whereas mutants in large t-antigen, which will complement hr-t, are denoted ts-a. FEATURES Location/Qualifiers source 1..5297 /organism="Murine polyomavirus strain A2" /mol_type="genomic DNA" /strain="A2" /db_xref="taxon:10636" repeat_region complement(join(1..22,5285..5297)) /note="replication origin associated 32 bp palindrome" stem_loop 1..22 /note="replication origen-associated 32 bp palindrome" protein_bind 41..45 /note="gaggc repeat; 71.24%" /bound_moiety="large T-antigen" variation 45..57 /note="cattgtagaggct in pya2; ct in pya3" /replace="ct" protein_bind 52..56 /note="gaggc repeat; 71.46%" /bound_moiety="large T-antigen" rep_origin complement(92..5270) /note="replication origin core element" protein_bind 95..99 /note="gaggc repeat; 72.27%" /bound_moiety="large T-antigen" prim_transcript 152..2930 /note="early mRNA (alt.; 3' end +/- 10bp)" prim_transcript 152..1500 /note="early mRNA (alt.; 3' end +/- 10bp)" protein_bind complement(154..158) /note="gaggc repeat; 73.38%" /bound_moiety="large T-antigen" protein_bind complement(165..169) /note="gaggc; 73.59%" /bound_moiety="large T-antigen" CDS join(175..411,797..2917) /codon_start=1 /product="large t-antigen" /protein_id="AAB59901.1" /translation="MDRVLSRADKERLLELLKLPRQLWGDFGRMQQAYKQQSLLLHPD KGGSHALMQELNSLWGTFKTEVYNLRMNLGGTGFQGSPPRTAERGTEESGHSPLHDDY WSFSYGSKYFTREWNDFFRKWDPSYQSPPKTAESSEQPDLFCYEEPLLSPNPSSPTDT PAHTAGRRRNPCVAEPDDSISPDPPRTPVSRKRPRPAGATGGGGGGVHANGGSVFGHP TGGTSTPAHPPPYHSQGGSESMGGSDSSGFAEGSFRSDPRCESENESYSQSCSQSSFN ATPPKKAREDPAPSDFPSSLTGYLSHAIYSNKTFPAFLVYSTKEKCKQLYDTIGKFRP EFKCLVHYEEGGMLFFLTMTKHRVSAVKNYCSKLCRSFLMCKAVTKPMECYQVVTAAP FQLITENKPGLHQFEFTDEPEEQKAVDWIMVADFALENNLDDPLLIMGYYLDFAKEVP SCIKCSKEETRLQIHWKNHRKHAENADLFLNCKAQKTICQQAAASLASRRLKLVECTR SQLLKERLQQSLLRLKELGSSDALLYLAGVAWYQCLLEDFPQTLFKMLKLLTENVPKR RNILFRGPVNSGKTGLAAALISLLGGKSLNINCPADKLAFELGVAQDQFVVCFEDVKG QIALNKQLQPGMGVANLDNLRTTWNGSVKVNLEKKHSNKRSQLFPPCVCTMNEYLLPQ TVWARFHMVLDFTCKPHLAQSLEKCEFLQRERIIQSGDTLALLLIWNFTSDVFDPDIQ GLVKEVRDQFASECSYSLFCDILCNVQEGDDPLKDICDIAEYTVY" CDS join(175..748,811..1502) /codon_start=1 /product="middle t-antigen" /protein_id="AAB59900.1" /translation="MDRVLSRADKERLLELLKLPRQLWGDFGRMQQAYKQQSLLLHPD KGGSHALMQELNSLWGTFKTEVYNLRMNLGGTGFQVRRLHADGWNLSTKDTFGDRYYQ RFCRMPLTCLVNVKYSSCSCILCLLRKQHRELKDKCDARCLVLGECFCLECYMQWFGT PTRDVLNLYADFIASMPIDWLDLDVHSVYNPKRRSEELRRAATVHYTMTTGHSAMEAS TSQGNGMISSESGTPATSRRLRLPSLLSNPTYSVMRSHSYPPTRVLQQIHPHILLEED EILVLLSPMTAYPRTPPELLYPESDQDQLEPLEEEEEEYMPMEDLYLDILPGEQVPQL IPPPIIPRAGLSPWEGLILRDLQRAHFDPILDASQRMRATHRAALRAHSMQRHLRRLG RTLLLVTFLAALLGICLMLFILIKRSRHF" CDS join(175..748,797..810) /codon_start=1 /product="small t-antigen" /protein_id="AAB59899.1" /translation="MDRVLSRADKERLLELLKLPRQLWGDFGRMQQAYKQQSLLLHPD KGGSHALMQELNSLWGTFKTEVYNLRMNLGGTGFQVRRLHADGWNLSTKDTFGDRYYQ RFCRMPLTCLVNVKYSSCSCILCLLRKQHRELKDKCDARCLVLGECFCLECYMQWFGT PTRDVLNLYADFIASMPIDWLDLDVHSVYNPRLSP" intron 412..796 /note="large t-antigen intron" intron 749..810 /note="middle t-antigen intron" intron 749..796 /note="small t-antigen intron" variation 1219 /note="g in pya2; a in pya3" /replace="a" regulatory 1478..1483 /regulatory_class="polyA_signal_sequence" /note="minor early mRNA polyadenylation signal; 98.38%" variation 1666..1668 /note="ccg in pya2; cagcgt in pya3" /replace="cagcgt" variation 2039..2046 /note="gcgagtct in pya2; gacggagtctt in pya3" /replace="gacggagtctt" variation 2494..2495 /note="ga in pya2; gga in pya3" /replace="gga" variation 2504..2505 /note="aa in pya2; a in pya3" /replace="a" variation 2892 /note="a in pya2; aa in pya3" /replace="aa" prim_transcript complement(2902..5078) /note="late mRNA" regulatory complement(2915..2920) /regulatory_class="polyA_signal_sequence" /note="late mRNA polyadenylation signal on comp strand; 25.51%" regulatory 2917..2922 /regulatory_class="polyA_signal_sequence" /note="major early mRNA polyadenylation signal; 25.54%" CDS complement(2925..4076) /codon_start=1 /product="VP1 (major capsid protein)" /protein_id="AAB59902.1" /translation="MAPKRKSGVSKCETKCTKACPRPAPVPKLLIKGGMEVLDLVTGP DSVTEIEAFLNPRMGQPPTPESLTEGGQYYGWSRGINLATSDTWIPRNNTLPTWSMAK SSFPCLNEDLTCDTLQMWEAVSVKTEVVGSGSLLDVHGFNKTHRFSKHKGNSTPVEGS QYHVFAGGGEPLDLQGLVTDARTKYKEEGVVTIKTITKKDMVNKDQVLNPISKAKLDK DGMYPVEIWHPDPAKNENTRYFGNYTGGTTAPPVLQFTNTLTTVLLDENGVGPLCKGE GLYLSCVDIMGWRVTRNYVSSLEKGFPRYFKITLRKRWVKNPYPMASLISSLFNNMLP QVQGQPMEGENTQVEEVRVYDGTEPVPGDPDMTRYVDRFGKTKTVFPGN" variation 3190..3194 /note="gacac in pya2; ggacatc in pya3" /replace="ggacatc" variation 3215 /note="a in pya2; t in pya3" /replace="t" variation 3332 /note="c in pya2; t in pya3" /replace="t" variation 3576..3577 /note="ac in pya2; ca in pya3" /replace="ca" variation 3618..3624 /note="atttcct in pya2; aattactt in pya3" /replace="aattactt" variation 3637 /note="a in pya2; t in pya3" /replace="t" variation 3649..3650 /note="tt in pya2; t in pya3" /replace="t" variation 3755..3756 /note="gg in pya2; g in pya3" /replace="g" variation 3770 /note="a in pya2; ag in pya3" /replace="ag" variation 3804..3806 /note="tcg in pya2; ttctg in pya3" /replace="ttctg" variation 3814..3817 /note="cacg in pya2; ctctg in pya3" /replace="ctctg" CDS complement(4045..5004) /codon_start=1 /product="VP2 (capsid protein)" /protein_id="AAB59904.1" /translation="MGAALTILVDLIEGLAEVSTLTGLSAEAILSGEALAALDGEITA LTLEGVMSSETALATMGISEEVYGFVSTVPVFVSRTAGAIWLMQTVQGASTISLGIQR YLHNEEVPTVNRNMALIPWRDPALLDIYFPGVNQFAHALNVVHDWGHGLLHSVGRYVW QMVVQETQHRLEGAVRELTVRQTHTFLDGLARLLENTRWVVSNAPQSAIDAINRGASS ASSGYSSLSDYYRQLGLNPPQRRALFNRIEGSMGNGGPTPAAHIQDESGEVIKFYQAQ VVSHQRVTPDWMLPLILGLYGDITPTWATVIEEDGPQKKKRRL" CDS complement(4045..4659) /codon_start=1 /product="VP3 (capsid protein)" /protein_id="AAB59903.1" /translation="MALIPWRDPALLDIYFPGVNQFAHALNVVHDWGHGLLHSVGRYV WQMVVQETQHRLEGAVRELTVRQTHTFLDGLARLLENTRWVVSNAPQSAIDAINRGAS SASSGYSSLSDYYRQLGLNPPQRRALFNRIEGSMGNGGPTPAAHIQDESGEVIKFYQA QVVSHQRVTPDWMLPLILGLYGDITPTWATVIEEDGPQKKKRRL" intron complement(4125..5022) /note="late mRNA (16S) intron; does not fit consensus" variation 4169..4170 /note="ga in pya2; g in pya3" /replace="g" variation 4181 /note="g in pya2; gg in pya3" /replace="gg" variation 4349 /note="g in pya2; a in pya3" /replace="a" intron complement(4710..5022) /note="late mRNA (18S) intron; does not fit consensus" variation 4772 /note="c in pya2; t in pya3" /replace="t" intron complement(5018..5022) /note="late mRNA (19S) intron; does not fit consensus" rep_origin 5044..5131 /note="replication origin alpha element" variation 5157 /note="a in pya2; g in pya3" /replace="g" rep_origin 5185..5269 /note="replication origin beta element" protein_bind complement(5221..5225) /note="gaggc repeat; 69.04%" /bound_moiety="large T-antigen" protein_bind complement(5234..5238) /note="taggc repeat; 69.28%" /bound_moiety="large T-antigen" CDS complement(5285..>5297) /codon_start=2 /product="ORF" /protein_id="AAG35191.1" /translation="ASA" protein_bind 5292..5296 /note="gaggc repeat; 70.38%" /bound_moiety="large T-antigen" BASE COUNT 1396 a 1270 c 1233 g 1398 t ORIGIN 1 gggggcccct ggcctccgct tactctggag aaaaagaaga gaggcattgt agaggcttcc 61 agaggcaact tgtcaaaaca ggactggcgc cttggaggcg ctgtggggcc acccaaattg 121 atataattaa gccccaaccg cctcttcccg cctcatttca gcctcaccac catcatggat 181 agagttctga gcagagctga caaagaaagg ctgctagaac ttctaaaact tcccagacaa 241 ctatgggggg attttggaag aatgcagcag gcatataagc agcagtcact gctactgcac 301 ccagacaaag gtggaagcca tgccttaatg caggaattga acagtctctg gggaacattt 361 aaaactgaag tatacaatct gagaatgaat ctaggaggaa ccggcttcca ggtaagaagg 421 ctacatgcgg atgggtggaa tctaagtacc aaagacacct ttggtgatag atactaccag 481 cggttctgca gaatgcctct tacctgccta gtaaatgtta aatacagctc atgtagttgt 541 atattatgcc tgcttagaaa gcaacataga gagctcaaag acaaatgtga tgccaggtgc 601 ctagtacttg gagaatgttt ttgtcttgaa tgttacatgc aatggtttgg aacaccaacc 661 cgagatgtgc tgaacctgta tgcagacttc attgcaagca tgcctataga ctggctggac 721 ctggatgtgc acagcgtgta taatccaagt aagtatcaag agggcgggtg ggtatttacg 781 gcctatattc ttacagggct ctccccctag aacggcggag cgaggaactg aggagagcgg 841 ccacagtcca ctacacgatg actactggtc attcagctat ggaagcaagt acttcacaag 901 ggaatggaat gatttcttca gaaagtggga ccccagctac cagtcgccgc ctaagactgc 961 cgagtcttct gagcaacccg acctattctg ttatgaggag ccactcctat cccccaaccc 1021 gagttctcca acagatacac ccgcacatac tgctggaaga agacgaaatc cttgtgttgc 1081 tgagcccgat gacagcatat ccccggaccc ccccagaact cctgtatcca gaaagcgacc 1141 aagaccagct ggagccactg gaggaggagg aggaggagta catgccaatg gaggatctgt 1201 atttggacat cctaccgggg gaacaagtac cccagctcat ccccccccct atcattccca 1261 gggcgggtct gagtccatgg gagggtctga ttcttcggga tttgcagagg gctcatttcg 1321 atccgatcct agatgcgagt cagagaatga gagctactca cagagctgct ctcagagctc 1381 attcaatgca acgccaccta agaaggctag ggaggaccct gctcctagtg actttcctag 1441 cagccttact gggtatttgt ctcatgctat ttattctaat aaaacgttcc cggcatttct 1501 agtatactcc accaaagaga aatgcaaaca attatatgat accataggga agttcaggcc 1561 cgaattcaaa tgcctggtcc attatgagga ggggggcatg ctgttctttc taactatgac 1621 taagcacagg gtttcagcag ttaagaatta ttgctctaag ctttgccgca gcttcctaat 1681 gtgtaaggca gtcaccaagc ctatggaatg ctatcaagtt gtaaccgcag caccatttca 1741 gttaataaca gaaaataagc caggcctcca ccaattcgag tttacagacg agccagaaga 1801 acagaaagca gtagactgga ttatggtagc agactttgca ctagaaaaca accttgatga 1861 tcccctgtta attatggggt attatcttga ttttgccaaa gaggttcctt catgcataaa 1921 gtgtagcaaa gaggaaaccc gcctccaaat acattggaaa aaccatagaa agcatgcaga 1981 gaatgcagac ctcttcctga attgtaaagc tcaaaagaca atctgtcagc aggcagctgc 2041 gagtctggca tccaggagac tgaaattagt agagtgtacc cgcagccagc tattaaagga 2101 gagattgcaa cagtctctcc tcaggctaaa agaacttggc tcctccgatg ctctactcta 2161 cctagcaggt gtcgcttggt accagtgtct tttagaggac tttcctcaaa ccctgtttaa 2221 gatgcttaag ctgctaacag aaaatgtgcc aaaacgacgc aacatacttt ttagaggacc 2281 agttaattca ggaaagacag gcctagcagc cgcgcttatt agcctgttag gaggcaagtc 2341 tctcaacata aattgccctg cagataaact tgcttttgag cttggtgtgg cacaggacca 2401 gtttgtggtc tgttttgaag atgtaaaggg tcaaatagcc ttgaacaaac aactgcagcc 2461 agggatggga gtggctaatc tagataatct caggactacc tggaatggga gtgtaaaggt 2521 caatctagaa aaaaagcaca gcaacaaaag gtcacaactc tttccaccct gtgtgtgtac 2581 aatgaatgaa tatctcctac cacaaacagt atgggcccgg tttcacatgg tgttggattt 2641 cacctgcaaa ccccatctgg cccaatctct tgaaaagtgt gaatttttgc aaagggaaag 2701 aattattcag agtggagata cccttgccct attactcata tggaatttca cttcagatgt 2761 atttgatcct gatattcagg gtcttgtgaa ggaggttcgt gaccagtttg ctagtgagtg 2821 ctcctacagt ttgttttgtg atatactttg taatgtgcaa gaaggcgacg accccttgaa 2881 ggacatatgt gatatagctg aatacacagt ttattgaata aacattaatt tccaggaaat 2941 acagtctttg tttttccaaa gcggtcaaca tagcgcgtca tatcagggtc ccccggtaca 3001 ggttcagtcc catcatacac tctaacctcc tctacctggg tgttctcccc ttccatgggt 3061 tggccctgca cttgggggag catgttgttg aaaagggaac ttatgaggga ggccatggga 3121 tagggatttt tgacccatct ttttctcagg gtgattttga aatatctggg aaagcccttc 3181 tccagtgatg acacatagtt tcttgtaact ctccagccca ttatatctac acaggagagg 3241 tataggccct ctcctttaca gaggggccca actccatttt catctaggag cacagttgtc 3301 agggtgtttg tgaactgcag gacgggtgga gctgttgtgc ctccagtgta attgccaaag 3361 taccttgtgt tctcattttt tgctggatct ggatgccaga tttcaactgg atacattccg 3421 tccttatcca gcttggcctt gctaattgga ttcaggactt ggtctttgtt gaccatgtcc 3481 ttctttgtga ttgttttgat tgttactacc ccttcttcct tgtattttgt tctggcatct 3541 gtcacaagtc cctggaggtc aagcggttcc ccgccaccag caaacacatg atattggctg 3601 ccttccactg gagtggaatt tcctttgtgt ttactgaatc tgtgggtttt gttgaaccca 3661 tgcacatcta acagtgagcc agagcccacc acctcggttt tcactgagac tgcctcccac 3721 atttgtaggg tgtcacaggt gaggtcctca ttgaggcatg ggaagctgga ctttgccata 3781 ctccatgtgg gaagtgtatt atttcgggga atccacgtat ctgatgtagc caaattaatc 3841 cctctgctcc aaccatagta ttgccctccc tctgttaggc tttcaggggt gggtggctgc 3901 cccattctgg ggttcagaaa agcttctatt tctgtcacac tgtctggccc tgtcacaagg 3961 tccagcacct ccataccccc tttaataagc agtttgggaa cgggtgcggg tcttggacag 4021 gcctttgtac attttgtctc gcatttagag acgccgcttt ttcttttggg ggccatcttc 4081 ctctatgact gttgcccaag taggtgtgat atcaccgtac agccctagaa ttaaaggaag 4141 catccagtca ggagtgactc tttggtggga caccacctgg gcctgataga actttatcac 4201 ctcacctgac tcatcctgta tatgtgctgc aggggtaggc ccaccatttc ccatgctccc 4261 ttctatgcga ttaaagagtg ctctcctctg tggtggatta agacctagtt gcctataata 4321 gtcacttagt gatgagtacc ctgagctcgc tgatgaggca cctctgttga ttgcatctat 4381 ggctgactga ggagcattag aaaccaccca ccgggtgttt tcaagtagcc tagctaggcc 4441 atccaggaat gtatgtgtct gtcttacagt tagttctctt acagctcctt ccagtctgtg 4501 ttgtgtttcc tgcacaacca tttgccacac atatcttccc acggaatgaa gtaggccatg 4561 gccccaatca tgtactacat ttagagcatg agcaaactga ttaactcctg ggaagtatat 4621 atcgagaagg gctggatccc gccatggtat caacgccata tttctattta cagtagggac 4681 ctcttcgttg tgtaggtacc gctgtattcc tagggaaata gtagaggcac cttgaactgt 4741 ctgcatcagc catatagccc ccgctgttcg acttacaaac acaggcacag tactgacaaa 4801 cccatacacc tcctctgaaa tacccatagt tgctagggct gtctccgaac tcattacacc 4861 ctccaaagtc agagctgtaa tttcgccatc aagggcagcg agggcttctc cagataaaat 4921 agcttctgcc gagagtcccg taagggtaga cacttcagct aatccctcga tgaggtctac 4981 tagaatagtc agtgcggctc ccattttgaa aattcactta cttgatcagc ttcagaagat 5041 ggcggagggc ctccaacaca gtaattttcc tcccgactct taaaatagaa aatgtcaagt 5101 cagttaagca ggaagtgact aactgaccgc agctggccgt gcgacatcct cttttaatta 5161 gttgctaggc aactgccctc cagagggcag tgtggttttg caagaggaag caaaaagcct 5221 ctccacccag gcctagaatg tttccaccca atcattacta tgacaacagc tgtttttttt 5281 agtattaagc agaggcc //