LOCUS TRN10TETR 4029 bp DNA linear UNA 08-JUL-2002 DEFINITION Transposon Tn10 tetracycline resistance and repressor genes tetA and tetR, complete cds. ACCESSION J01830 K00615 K01493 VERSION J01830.1 KEYWORDS drug resistance protein; insertion sequence; tetracycline repressor protein; tetracycline resistance; transposon; unidentified reading frame. SOURCE Transposon Tn10 ORGANISM Transposon Tn10 other sequences; transposons. REFERENCE 1 (bases 1458 to 1659) AUTHORS Bertrand,K.P., Postle,K., Wray,L.V. Jr. and Reznikoff,W.S. TITLE Overlapping divergent promoters control expression of Tn10 tetracycline resistance JOURNAL Gene 23 (2), 149-156 (1983) PUBMED 6311683 REFERENCE 2 (bases 1548 to 3077) AUTHORS Nguyen,T.T., Postle,K. and Bertrand,K.P. TITLE Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322 JOURNAL Gene 25 (1), 83-92 (1983) PUBMED 6319234 REFERENCE 3 (bases 1518 to 1619) AUTHORS Wray,L.V. Jr. and Reznikoff,W.S. TITLE Identification of repressor binding sites controlling expression of tetracycline resistance encoded by Tn10 JOURNAL J. Bacteriol. 156 (3), 1188-1191 (1983) PUBMED 6315680 REFERENCE 5 (bases 3072 to 4029) AUTHORS Schollmeier,K. and Hillen,W. TITLE Transposon Tn10 contains two structural genes with opposite polarity between tetA and IS10R JOURNAL J. Bacteriol. 160 (2), 499-503 (1984) PUBMED 6094471 REFERENCE 6 (bases 1 to 1550) AUTHORS Postle,K., Nguyen,T.T. and Bertrand,K.P. TITLE Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant JOURNAL Nucleic Acids Res. 12 (12), 4849-4863 (1984) PUBMED 6330687 REFERENCE 7 (bases 1 to 4029) AUTHORS Hillen,W. and Schollmeier,K. TITLE Nucleotide sequence of the Tn10 encoded tetracycline resistance gene JOURNAL Nucleic Acids Res. 11 (2), 525-539 (1983) PUBMED 6298728 COMMENT Sequence computer readable form kindly provided by W.Hillen, 29-MAR-1985. Open reading frames in the 3' flank of the tetR gene are located at positions 585-442 ('gtg' start codon) and 815-681 [6]. The genes for tetA and tetR are transcribed in opposite directions from promoters in the regulatory region (tetA -35 and -10 regions are located at positions 1546-1551 and 1570-1575 respectively and for tetR at 1579-1574 and 1556-1551) [1]. Their ribosome binding sites are found at 1595-1599 and 1537-1533. The physical overlap between the tetA and tetR promoters may mean that RNA polymerase interaction with these promoters is mutually exclusive [1]. The expression of tetA and tetR is regulated by tetracycline and the tetR repressor [1]. If the repressor binding site at positions 1561-1569 is deleted, a higher level of derepression is noted than is the case when the second repressor binding site (1591-1599) is deleted [3]. ORFL and ORFR also are transcribed in opposite directions and have only 18 bp between their mRNA start sites [5]. -35 and -10 regions for ORFR are located at positions 3499-3504 and 3504-3572 and for ORFL at 3559-3554 and 3539-3534 respectively [5]. The start of ORFL could be the 'gtg' codon at position 3387-3385 [5]. Though no function for the two ORFs is presently known, they may be tetracycline-inducible proteins. Complete source information: Tn10 (transposon Tn10) DNA, clone pRT29 [1]; clone lambda-RT301 [3]; clone pRT61 [4],[5]; clone pBT107 [2]; clone pBT402 [6]. FEATURES Location/Qualifiers source 1..4029 /organism="Transposon Tn10" /mol_type="genomic DNA" /db_xref="taxon:2389" mobile_element 1..4029 /mobile_element_type="transposon:Tn10" gene complement(<903..1544) /gene="tetR" mRNA complement(<903..1544) /gene="tetR" /product="tetracycline repressor protein" CDS complement(903..1526) /gene="tetR" /codon_start=1 /transl_table=11 /product="tetracycline repressor protein" /protein_id="AAB59093.1" /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" misc_binding 1561..1569 /bound_moiety="repressor binding site A" gene 1581..>2813 /gene="tetA" mRNA 1581..>2813 /gene="tetA" /product="tetracycline resistance protein" misc_binding 1591..1599 /gene="tetA" /bound_moiety="repressor binding site B" CDS 1608..2813 /gene="tetA" /codon_start=1 /transl_table=11 /product="tetracycline resistance protein" /protein_id="AAB59094.1" /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT AVLLEFIADSSAFAFLAFISEGWLDFPVLILLAGGGIALPALQGVMSIQTKSHEQGAL QGLLVSLTNATGVIGPLLFTVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA QGSKQETSA" misc_difference 2449 /gene="tetA" /note="conflict" /citation=[2] /replace="" misc_difference 2509 /gene="tetA" /note="conflict" /citation=[2] /replace="" misc_difference 2595 /gene="tetA" /note="conflict" /citation=[2] /replace="" misc_difference 2667 /gene="tetA" /note="conflict" /citation=[2] /replace="" mRNA complement(<2926..3549) /product="ORFL" CDS complement(2926..3519) /note="ORF L; putative" /codon_start=1 /transl_table=11 /product="unknown" /protein_id="AAB59095.1" /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDSDVLKNLLN GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK" misc_difference 3065..3066 /note="conflict" /citation=[2] /replace="" mRNA 3532..>4023 /product="ORFR" /note="major alt." mRNA 3568..>4023 /product="ORFR" /note="minor alt." CDS 3607..4023 /note="ORF R" /codon_start=1 /transl_table=11 /product="unknown" /protein_id="AAB59096.1" /translation="MYIEQHSRYQNKANNIQLEYDDRQFHTTVIKDVLLWIEHNLDQS LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK YQFDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAMH" BASE COUNT 1121 a 738 c 844 g 1326 t ORIGIN 1 ctaaaatagc gctagagcaa gctggactgt tagtacttgc gatccggaca ccattaattc 61 gtaagttaaa acaggaaaaa ccgggggaac ttggcgaaat agcacgagta ttggcggaga 121 ataacattaa tattttagtg caatacagtg accatgctaa ccaactgata ttaataacgg 181 acaatgatag tatggctgca tctgttacgc tcccttgggc aataaagtga acttgcgatg 241 gctaatttaa tacgaaaaga ggttaccttt gagtcctcaa tagccgcgat agggggctca 301 tgtctgacat ttcacgagtt aaaatactca gtgctttgat ggatgggcga gcttggacgg 361 ccactgagct aagttctgtg gcgaatatat cagcttcaac ggcgagcagt catttatcta 421 aattattaga ttgccagcta atcacagtag tagctcaagg caagcatcgt tattttcggc 481 tagcaggaaa agatattgct gaattgatgg aaagtatgat ggggatctcc ttaaaccatg 541 gcgtacatgc caaagtttcc acgccagtgc atttacgaaa agcacgtact tgctatatga 601 tcatttagct ggcgaagttg ccgttaagat ctatgattcc ctttgtcaac agcaatggat 661 cactgaaaat ggttcaatga tcacattaag tggtattcaa tattttcatg aaatgggaat 721 tgacgttcct tccaaacatt cacgtaaaat ctgttgtgcg tgtttagatt ggagtgaacg 781 ccgtttccat ttaggtgggt acgttggagc cgcattattt tcgctttatg aatctaaagg 841 gtggttaact cgacatcttg gttaccgtga agttaccatc acggaaaaag gttatgctgc 901 ttttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat caattcaagg 961 ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat 1021 aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac ttgatgctct 1081 tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc atataatgca 1141 ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag agtttcatac 1201 tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg acttagtaaa 1261 gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc cccttctaaa 1321 gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc 1381 cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt ctgggcgagt 1441 ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc gctgttaatc 1501 actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac actctatcat 1561 tgatagagtt attttaccac tccctatcag tgatagagaa aagtgaaatg aatagttcga 1621 caaagatcgc attggtaatt acgttactcg atgccatggg gattggcctt atcatgccag 1681 tcttgccaac gttattacgt gaatttattg cttcggaaga tatcgctaac cactttggcg 1741 tattgcttgc actttatgcg ttaatgcagg ttatctttgc tccttggctt ggaaaaatgt 1801 ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca tcgctggatt 1861 acttattgct ggctttttca agtgcgcttt ggatgctgta tttaggccgt ttgctttcag 1921 ggatcacagg agctactggg gctgtcgcgg catcggtcat tgccgatacc acctcagctt 1981 ctcaacgcgt gaagtggttc ggttggttag gggcaagttt tgggcttggt ttaatagcgg 2041 ggcctattat tggtggtttt gcaggagaga tttcaccgca tagtcccttt tttatcgctg 2101 cgttgctaaa tattgtcact ttccttgtgg ttatgttttg gttccgtgaa accaaaaata 2161 cacgtgataa tacagatacc gaagtagggg ttgagacgca atcgaattcg gtatacatca 2221 ctttatttaa aacgatgccc attttgttga ttatttattt ttcagcgcaa ttgataggcc 2281 aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg aatagcatga 2341 tggttggctt ttcattagcg ggtcttggtc ttttacactc agtattccaa gcctttgtgg 2401 caggaagaat agccactaaa tggggcgaaa aaacggcagt actgctcgaa tttattgcag 2461 atagtagtgc atttgccttt ttagcgttta tatctgaagg ttggttagat ttccctgttt 2521 taattttatt ggctggtggt gggatcgctt tacctgcatt acagggagtg atgtctatcc 2581 aaacaaagag tcatgagcaa ggtgctttac agggattatt ggtgagcctt accaatgcaa 2641 ccggtgttat tggcccatta ctgtttactg ttatttataa tcattcacta ccaatttggg 2701 atggctggat ttggattatt ggtttagcgt tttactgtat tattatcctg ctatcgatga 2761 ccttcatgtt aacccctcaa gctcagggga gtaaacagga gacaagtgct tagttatttc 2821 gtcaccaaat gatgttattc cgcgaaatat aatgaccctc ttgataaccc aagagggcat 2881 tttttacgat aaagaagatt tagcttcaaa taaaacctat ctattttatt tatctttcaa 2941 gctcaataaa aagccgcggt aaatagcaat aaattggcct tttttatcgg caagctcttt 3001 taggtttttc gcatgtattg cgatatgcat aaaccagcca ttgagtaagt ttttaagcac 3061 atcactatca taagctttaa gttggttctc ttggatcaat ttgctgacaa tggcgtttac 3121 cttaccagta atgtattcaa ggctaatttt ttcaagttca ttccaaccaa tgataggcat 3181 cacttcttgg atagggataa ggtttttatt attatcaata atataatcaa gataatgttc 3241 aaatatactt tctaaggcag accaaccatt tgttaaatca gtttttgttg tgatgtaggc 3301 atcaatcata attaattgct gcttataaca ggcactgagt aattgttttt tatttttaaa 3361 gtgatgataa aaggcacctt tggtcaccaa cgcttttccc gagatctcat ctattgaaac 3421 agcttgatag cctttttcaa caaacaatat tcgtgctgag ttaaccagtg attgataggt 3481 actcttaaaa ttttcttgtt gatgattttt attttccatg atagatttaa aataacatac 3541 cgtcagtatg tttatggtat catgatgatg tggtcgtgac aatcttaaga acatttaggt 3601 tattttatgt atattgaaca gcattctcgc tatcaaaata aagctaataa catccaatta 3661 gaatatgatg atagacagtt tcatacaacg gttatcaaag atgttctatt atggattgaa 3721 cataatttag atcagtcttt actgcttgat gatgtggcga ataaagcggg ttataccaag 3781 tggtattttc agcggctgtt caaaaaagta acaggggtca cactggctag ctatattcgt 3841 gctcgtcgtt tgacgaaagc ggctgttgag ttgaggttga cgaaaaaaac tatccttgag 3901 atcgcattaa aatatcaatt tgattcccaa caatctttta cacgtcgatt taagtacatt 3961 tttaaggtta caccaagtta ttatcggcgt aataaattat gggaattgga ggcaatgcac 4021 tgagagatc //