LOCUS       HQ336798                1945 bp    mRNA    linear   PLN 04-APR-2011
DEFINITION  Picea sitchensis genotype H898 (+)-3-carene synthase (TPS-3car1)
            mRNA, complete cds.
ACCESSION   HQ336798
VERSION     HQ336798.1
KEYWORDS    .
SOURCE      Picea sitchensis (Sitka spruce)
  ORGANISM  Picea sitchensis
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae;
            Picea.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Hall,D.E., Robert,J.A., Keeling,C.I., Domanski,D., Quesada,A.L.,
            Jancsik,S., Kuzyk,M.A., Hamberger,B., Borchers,C.H. and Bohlmann,J.
  TITLE     An integrated genomic, proteomic and biochemical analysis of
            (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis)
            genotypes that are resistant or susceptible to white pine weevil
  JOURNAL   Plant J. 65 (6), 936-948 (2011)
   PUBMED   21323772
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Hall,D., Robert,J., Keeling,C.I., Domanski,D., Lara Quesada,A.,
            Jancsik,S., Kuzyk,M., Hamberger,B., Borchers,C. and Bohlmann,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2010) Michael Smith Laboratories, University of
            British Columbia, 301-2185 East Mall, Vancouver, BC V6T 1Z4, Canada
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Picea sitchensis"
                     /mol_type="mRNA"
                     /db_xref="taxon:3332"
                     /note="resistant;
                     genotype: H898"
     gene            1..1945
                     /gene="TPS-3car1"
                     /note="PsTPS-3car1"
     CDS             1..1884
                     /gene="TPS-3car1"
                     /function="catalyzes the cyclization of geranyl
                     diphosphate to (+)-3-carene"
                     /note="PsTPS-3car1_R"
                     /codon_start=1
                     /product="(+)-3-carene synthase"
                     /protein_id="ADU85924.1"
                     /translation="MSVISIVPLASKPCLYKSFISSTHEPKALRRPISTVGLCRRAKS
                     VTASMSMSSSTALSDDGVQRRIGNHHSNLWDDNFIQSLSSPYGASSYAERAERLIGEV
                     KEIFNRISMANGELVSHVDDLLQHLSMVDNVERLGIDRHFQTEIKVSLDYVYSYWSEK
                     GIGPGRDIVCADLNTTALGFRLLRLHGYTMFPDVFEQFKDQMGRIACSTNQTERQISS
                     ILNLFRASLIAFPWEKVMEEAEIFSTAYLKEALQTIPVSSLSREIQYVLDYRWHSDLP
                     RLETRTYIDILRENATNETLDMKTEKLLELAKVEFNIFNSLQQNELKCVSRWWKESGS
                     PDLTFIRHRQVEFYTLVSGIDMEPKRSTFRINFVKICHFVTILDDMYDTFGTIDELRL
                     FTAAVKRWDKSATECLPEYMKGVYIDLYETVNELAREAYKSQGRDTLNYARQALEDYL
                     GSYLKEAEWISTGYIPTFEEYLVNGKVSSAHRIATLQPILMLDVPFPPHVLQEIDFPS
                     KFNDLAGSILRLRGDTRCYQNDRARGEEASCISCYMKDNPGSTEEDALNHINGMIEKQ
                     IKELNWELLKPDKNVPISSKKHAFNISRGLHHFYKYRDGYTVANSETRNLVIKTVLEP
                     VPM"
BASE COUNT          590 a          401 c          404 g          550 t
ORIGIN      
        1 atgtctgtta tttccatcgt gccgttggct tccaaaccgt gcctttacaa atcgtttatc
       61 agttctactc atgagcctaa ggctctccgt agaccaatct caactgttgg actgtgtagg
      121 cgagcgaaat ctgtcacggc ttccatgagc atgagttcga gcactgccct atctgatgat
      181 ggtgtacaaa gacgcatagg caatcatcat tccaacctgt gggacgataa tttcatacaa
      241 tctctctcat caccttatgg ggcatcttct tacgctgaac gtgctgagag actcattggg
      301 gaagtaaagg agattttcaa ccgaatttct atggcaaatg gagaattagt gagtcatgtc
      361 gatgatctcc ttcaacacct ttcgatggtc gataacgttg aacgattggg gatcgacaga
      421 catttccaaa ctgagatcaa agtttccctc gattatgttt acagttactg gagcgaaaaa
      481 ggcattggac ctgggagaga tattgtttgt gctgatctca acaccactgc cttggggttt
      541 cgacttcttc gactacatgg atatactatg tttccagacg tgtttgaaca attcaaagac
      601 cagatggggc ggattgcctg ttccaccaat cagacagaga ggcagatcag cagcattctg
      661 aatttatttc gggcttctct cattgctttt ccctgggaaa aagttatgga agaggctgaa
      721 atcttctcga ctgcatattt aaaagaagcc ctacaaacga ttccagtctc cagtctttca
      781 cgagagatac agtacgttct cgactatcgt tggcactcag atttgcctag attggaaact
      841 aggacttaca tcgacatact tcgagagaat gctacaaatg aaactctaga tatgaaaact
      901 gagaaacttt tagaacttgc aaaagtggag ttcaatatct tcaactcttt acaacaaaat
      961 gagttaaaat gtgtctccag atggtggaaa gaatcaggtt cccctgatct aaccttcatt
     1021 cgacatcgtc aagtggaatt ctacactttg gtctctggca ttgacatgga gcctaaacgt
     1081 tctacattca gaattaactt tgtcaaaata tgtcattttg tcacaatttt ggatgatatg
     1141 tatgacacct ttgggacaat cgatgagctc cgactgttca cagctgcagt taaaagatgg
     1201 gataagtcgg cgacagaatg tcttccagaa tatatgaaag gagtgtacat agatctttat
     1261 gaaactgtaa atgaattggc gcgagaggca tataagtcac aaggccgaga cacgctcaac
     1321 tatgctcgac aggcgttgga ggattatctt ggttcgtatc ttaaagaagc agagtggatc
     1381 tccacaggtt atataccaac atttgaggag tacttagtga atgggaaagt tagttctgct
     1441 catcgcatag caacattgca acccatactc atgttggacg ttccctttcc tcctcacgtc
     1501 ctacaggaaa ttgactttcc gtctaagttc aatgacttgg caggttccat ccttcgatta
     1561 cgaggtgaca cacggtgtta ccagaatgat agggctcgtg gagaagaagc ttcttgtata
     1621 tcatgctata tgaaagacaa tcctggatca acagaagaag atgctctcaa tcatatcaat
     1681 ggcatgattg aaaaacaaat caaagaatta aattgggagc ttcttaaacc agacaaaaat
     1741 gttccaatct cttccaagaa acatgctttt aacataagca gaggtttgca ccacttctac
     1801 aaataccgag atggatacac cgttgccaac agtgagacaa ggaatttggt gataaaaacc
     1861 gttcttgaac ctgtgcctat gtaaccacat atcaaaatat catatttaca ataagttgat
     1921 acttatttta ggctaaaaaa aaaaa
//