LOCUS       HQ147787                 789 bp    DNA     linear   BCT 14-MAR-2012
DEFINITION  Thermobifida fusca culture-collection DSM:44342 cutinase 1 (cut1)
            gene, partial cds.
ACCESSION   HQ147787
VERSION     HQ147787.1
KEYWORDS    .
SOURCE      Thermobifida fusca
  ORGANISM  Thermobifida fusca
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Thermobifida.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Herrero-Acero,E., Ribitsch,D., Steinkellner,G., Gruber,K.,
            Greimel,K., Eiteljoerg,I., Trotscha,E., Wei,R., Zimmermann,W.,
            Zinn,M., Cavaco-Paulo,A., Freddi,G., Schwab,H. and Guebitz,G.
  TITLE     Enzymatic Surface Hydrolysis of PET: Effect of Structural Diversity
            on Kinetic Properties of Cutinases from Thermobifida
  JOURNAL   Macromolecules 44 (12), 4632-4640 (2011)
REFERENCE   2  (bases 1 to 789)
  AUTHORS   Ribitsch,D., Herrero-Acero,E., Greimel,K., Trotscha,E., Schwab,H.
            and Guebitz,G.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2010) Enzyme Development, Austrian Centre of
            Industrial Biotechnology (ACIB), Petersgasse 14, Graz, Styria 8010,
            Austria
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Thermobifida fusca"
                     /mol_type="genomic DNA"
                     /culture_collection="DSM:44342"
                     /db_xref="taxon:2021"
     gene            <1..789
                     /gene="cut1"
     CDS             <1..789
                     /gene="cut1"
                     /note="Thf42_Cut1"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase 1"
                     /protein_id="ADV92528.1"
                     /translation="MANPYERGPNPTDALLEARSGPFSVSEENVSRLSASGFGGGTIY
                     YPRENNTYGAVAISPGYTGTEASIAWLGERIASHGFVVITIDTITTLDQPDSRAEQLN
                     AALNHMINRASSTVRSRIDSSRLAVMGHSMGGGGSLRLASQRPDLKAAIPLTPWHLNK
                     NWSSVRVPTLIIGADLDTIAPVLTHARPFYNSLPTSISKAYLELDGATHFAPNIPNKI
                     IGKYSVAWLKRFVDNDTRYTQFLCPGPRDGLFGEVEEYRSTCPF"
BASE COUNT          146 a          319 c          211 g          113 t
ORIGIN      
        1 atggccaacc cctacgagcg cggccccaac ccgaccgacg ccctgctcga agcccgcagc
       61 ggccccttct ccgtcagcga ggagaacgtc tcccggttga gcgccagcgg cttcggcggc
      121 ggcaccatct actacccgcg ggagaacaac acctacggtg cggtggcgat ctcccccggc
      181 tacaccggca ctgaggcttc catcgcctgg ctgggcgagc gcatcgcctc ccacggcttc
      241 gtcgtcatca ccatcgacac catcaccacc ctcgaccagc cggacagccg ggcagagcag
      301 ctcaacgccg cgctgaacca catgatcaac cgggcgtcct ccacggtgcg cagccggatc
      361 gacagcagcc gactggcggt catgggccac tccatgggcg gcggcggcag cctgcgtctg
      421 gcctcccagc gtcccgacct gaaggccgcc atcccgctca ccccgtggca cctcaacaag
      481 aactggagca gtgtgcgggt tcccaccctc atcatcggtg ctgacctgga caccatcgct
      541 ccggtcctca cccacgcccg gcccttctac aacagcctcc cgacctcgat cagcaaggcc
      601 tacctggagc tggacggcgc aacccacttc gccccgaaca tccccaacaa gatcatcggc
      661 aagtacagcg tcgcctggct caagcggttc gtcgacaacg acacccgcta cacccagttc
      721 ctctgccccg gaccgcgcga cggactcttc ggcgaggtcg aagagtaccg ctccacctgc
      781 ccgttctga
//