LOCUS       H79045                   443 bp    mRNA    linear   EST 09-NOV-1995
DEFINITION  yu13h03.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:233717 3' similar to gb:M29399 ERYTHROCYTE MEMBRANE PROTEIN
            BAND 4.2 (HUMAN), mRNA sequence.
ACCESSION   H79045
VERSION     H79045.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 1334
            High quality sequence stops: 337
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1334   Std Error: 0.00
            Seq primer: Promega -21m13
            High quality sequence stop: 337.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3786894"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:233717"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
BASE COUNT           86 a           98 c          133 g          121 t
ORIGIN      
        1 ttttttcggc taccagctct cacctacacc cacgnttggt ttagattgta gaacaagggt
       61 tggcaggaga gtggtgatag agctggaagt ttaagctgat agttcagggg ctaccacggt
      121 gacgctttta tagttggtta ggttctggaa catgttgcag tccacttcca cagtgagtct
      181 ctggagcccc acatgtgttg gcgtgaactg gaacttggca cacatggtnt tttcaggcca
      241 cactgaacgg aatctgtagc tcctctctct gtgaatgagc ccccttcccg ggatgggaga
      301 tcacacagtc ctccatgggg gggcatctag gggagttctg ggagggctga ctgagggctn
      361 tgagggggtt gatactgctc tgctttctct gggcatcttg atgggcaagg tttgggtcta
      421 caantgggca atgtcttncc ccg
//