LOCUS       H03183                   234 bp    mRNA    linear   EST 20-JUN-1995
DEFINITION  yj47a08.r1 Soares placenta Nb2HP Homo sapiens cDNA clone
            IMAGE:151862 5', mRNA sequence.
ACCESSION   H03183
VERSION     H03183.1
DBLINK      BioSample: SAMN00154885
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 667
            High qality sequence stops: 219
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 667   Std Error: 0.00
            Seq primer: M13RP1
            High quality sequence stop: 219.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:564007"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:151862"
                     /sex="female"
                     /clone_lib="SAMN00154885 Soares placenta Nb2HP"
                     /dev_stage="placenta obtained at birth (full term)"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: placenta; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer [5'
                     AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
BASE COUNT           40 a           47 c           20 g          124 t
ORIGIN      
        1 tcagtgctat tttatccaat ttttggcttt atatttttct atcttctata cttctccaat
       61 acttgtctta gcttgttttt cattttctat ctgaaactct tgacaatatt ttcattttct
      121 atctngtttc tatcttccaa ttttcttcta agtttgtaca tttngccctt agctttttgt
      181 ttcctagctt gtctttnttc ttctgcttcc tacttttcag gtttaaattt atct
//