LOCUS       GO515252                 463 bp    mRNA    linear   EST 14-APR-2009
DEFINITION  Mdrtb1020L22.g1 Apple_EST_Mdrtb Malus domestica cDNA 5' similar to
            pir|T00044 vacuolar sorting receptor protein homolog PV72 -
            cucurbit dbj|BAA25079.1| PV72 [Cucurbita cv. Kurokawa Amakuri],
            mRNA sequence.
ACCESSION   GO515252
VERSION     GO515252.1
DBLINK      BioSample: SAMN00167513
KEYWORDS    EST.
SOURCE      Malus domestica (apple)
  ORGANISM  Malus domestica
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae;
            Maleae; Malus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Korban,S., Vodkin,L., Liu,L., Gasic,K., Gonzales,O., Hernandez,A.,
            Aldwinckle,H., Malnoy,M., Carroll,N., Goldsbrough,P., Orvis,K.,
            Clifton,S., Pape,D., Marra,M., Hillier,L., Martin,J., Wylie,T.,
            Dante,M., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Ronko,I.,
            Tsagareishvili,R., Kennedy,S., Waterston,R. and Wilson,R.
  TITLE     Apple Functional Genomics grant - NSF 0321702
  JOURNAL   Unpublished
COMMENT     Contact: Schuyler S. Korban
            Apple Functional Genomics grant - NSF 0321702
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material provided by S.Korban/G. Fazio Library constructed
            by K. Gasic/S. Korban Library sequenced by Washington University
            Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 101
            original value before phred recall for SR was 869
            Seq primer: -40UP.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Malus domestica"
                     /mol_type="mRNA"
                     /cultivar="M.111 rootstock"
                     /db_xref="taxon:3750"
                     /tissue_type="Root"
                     /clone_lib="SAMN00167513 Apple_EST_Mdrtb"
                     /dev_stage="young root"
                     /lab_host="DH10B ampicillin resistant"
                     /note="Vector: pBluescript II SK (+); Site_1: Not I;
                     Site_2: EcoRI; Total RNA was extracted separately from
                     whole root using the 'pine tree' method. Poly(A)+mRNA was
                     isolated twice from total RNA from each stage using the
                     Oligotex Direct mRNA kit (Qiagen). mRNA was reverse
                     transcribed into double stranded cDNA using a modified
                     oligo18(dT) primer with an identifying tag sequence. [Tag
                     identification when sequencing from 5' end: insert
                     18(A)TCGTG] Double stranded cDNAs were size selected (more
                     than 450 bp), adaptored with EcoRI adapters at both ends
                     and then digested with NotI. The cDNAs were then
                     directionally cloned into EcoR1-NotI digested pBS II SK(+)
                     phagemid vector(Stratagene). The total number of white
                     colony forming units (cfu) was 7.4 x 10^6 cfu (colony
                     forming units). The background of empty clones was less
                     than 3%. Inserts ranged from 0.5kb to 3kb, as determined
                     by PCR."
BASE COUNT          126 a           82 c          147 g          108 t
ORIGIN      
        1 ttgctgtcgc aaatgcttgc agttgtccac caggattcaa gggtgatgga gagaaaacat
       61 gtgaagatgt tgatgagtgc aaggagaaaa cgtcttgcca atgttcacaa tgcaaatgca
      121 agaatacttg gggaagttac gagtgcagtt gcggtggcgg tttactgtac atgcgagaaa
      181 atgatgcatg tataggtaaa aacggaagtg gttctgggtc tagctggggc ctaatttggg
      241 ttatcattct ctgcttgggt gctgtcggag ttgggggctt cgcagtttac aagtaccgaa
      301 tccggagata catggattcg gagatccggg caatcatggc gcagtacatg ccgttggatg
      361 ggggaggaga agttcccagc cacgtcgccc gcggggatat ctgaaaccgg cggagagacg
      421 tattcataaa acataagatg ctgtccgaga gaaaagggaa gac
//