LOCUS GABD01009600 1676 bp mRNA linear TSA 31-OCT-2012 DEFINITION TSA: Pan troglodytes Chimpanzee_0098511 mRNA sequence. ACCESSION GABD01009600 VERSION GABD01009600.1 DBLINK BioProject: PRJNA173089 Sequence Read Archive: SRR544870 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Pan troglodytes (chimpanzee) ORGANISM Pan troglodytes Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Pan. REFERENCE 1 (bases 1 to 1676) AUTHORS Maudhoo,M.D., Meehan,D.T. and Norgren,R.B. Jr. TITLE De novo assembly of the reference chimpanzee transcriptome from NextGen mRNA sequences JOURNAL Unpublished REFERENCE 2 (bases 1 to 1676) AUTHORS Maudhoo,M.D., Meehan,D.T. and Norgren,R.B. Jr. TITLE Direct Submission JOURNAL Submitted (15-OCT-2012) Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, 985805 Nebraska Medical Center, Omaha, NE 68198-5805, USA COMMENT Paired-end, 101 bp sequencing was performed on the Illumina platform. All reads were aligned with the human RefSeq mRNA transcripts using BLAST. Any reads with an alignment length difference with respect to the human reference sequence of 2 or greater were removed prior to assembly. For a given pair of reads, if one sequence was filtered, its pair was also filtered. Velveth was performed as recommended. kmer was set at 31. The output files from Velveth were passed to Velvetg. After performing Velvetg, this output file was passed to Oases where the final assembly was performed. Default parameters were used for Velvetg and Oases. ##Assembly-Data-START## Assembly Method :: Velvet 1.2.07; Oases 0.2.08 Sequencing Technology :: Illumina HiSeq ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1676 /organism="Pan troglodytes" /mol_type="mRNA" /isolation_source="Clint" /db_xref="taxon:9598" /cell_line="untransformed fibroblast cells" /tissue_type="skin" gene 1..1676 /gene="SS18L1" CDS 41..1231 /gene="SS18L1" /codon_start=1 /product="synovial sarcoma translocation gene on chromosome 18-like 1" /protein_id="JAA23500.1" /db_xref="GI:410289800" /translation="MSVAFASARPRGKGEVTQQTIQKMLDENHHLIQCILEYQSKGKT AECTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQ GSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQ GVPMQGQGTIGNYVSRTNINMQSNPVSMMQQQAATSHYSSAQGGSQHYQGQSSIAMMG QGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGH GDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPS QQSYPGQQQGYGPAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYG NYQQ" BASE COUNT 355 a 553 c 484 g 284 t ORIGIN 1 tccacctcga tgaccacggg ctgagccccg cgccgccacc atgtccgtgg ccttcgcgtc 61 tgcccggcca agaggcaaag gggaggttac gcagcaaacc atccagaaga tgctggacga 121 gaaccaccac ctgatccagt gcatcctgga gtaccagagc aagggcaaga cggccgagtg 181 cacgcagtac cagcagatcc tgcaccggaa cctggtatac ctggccacga tcgcagactc 241 caaccagaac atgcagtccc tgcttcctgc cccgcccacg cagaacatga acctgggccc 301 tggagccctg actcagagcg gctccagcca gggcctgcac tctcagggca gcctgagtga 361 cgccatcagc acgggcctgc caccttcctc cctcctgcag ggccagattg gcaacgggcc 421 gagccacgtg tccatgcagc agacggcgcc taacacgctg cccaccacct ccatgagcat 481 ctctgggccc ggctacagcc acgcgggacc cgcctcgcag ggcgtcccca tgcaggggca 541 aggcaccatc ggcaactacg tgtctcggac caacatcaac atgcagtcca acccagtctc 601 catgatgcag cagcaggcgg ccacgtcgca ctacagctcg gcgcagggcg gcagccagca 661 ctaccagggc cagtcgtcca tcgccatgat ggggcagggc agccagggga gcagcatgat 721 ggggcagcgg cccatggcgc cctaccggcc ctcccagcaa ggctcttccc agcagtacct 781 gggccaggag gagtactatg gcgagcagta cagccacagc cagggcgccg cggagcccat 841 gggccagcag tactaccccg acggccacgg cgattacgcc taccagcagt catcctacac 901 ggagcagagc tacgaccggt cctttgagga gtccacgcag cactactatg aggggggaaa 961 ctcccagtac agccagcagc aggccgggta ccagcagggt gccgcgcagc agcagacgta 1021 ctcccagcag cagtacccca gccagcagag ctaccccggg cagcagcagg gctacgggcc 1081 tgcccaggga gccccatcac agtaccccgg ctaccagcaa ggccaaggcc agcagtacgg 1141 aagctaccga gcaccgcaga cagcgccgtc tgcccagcag cagcggccct acggctatga 1201 acagggccag tatggaaatt accagcagta agggacacac attctggctg gagcccttgt 1261 ggtagcgtgt tcatccaggg gccggatggg ctggcggcag ctctggtgaa ttgtgacatg 1321 ttggttacct gttcgcccag tgccacgtct gcatgtgaag cgtgctcatt tcatgctggg 1381 tatgacgccg agcgcacacc actggcgtga gacagcgctt ggtggtgtga tacttttggt 1441 gctgtgtata gtattgtatg tcggtacacg gagaggtatc ctttttttgt cccccgcccc 1501 cttctcaatg tttctagcta gctttggggg tcattttgtc atcagagcat tctgtgccca 1561 gggacaggac agatctcaag gacaccacag tccacctgtt cccgtcaaca gaccttaggt 1621 ctcattttcc tcctcatgca gtgttgtagt gtgggttgtc aacttttctt taactg //