LOCUS       G11316                   234 bp    DNA     linear   STS 30-MAR-2000
DEFINITION  SHGC-9776 Human Homo sapiens STS genomic, sequence tagged site.
ACCESSION   G11316
VERSION     G11316.1
KEYWORDS    STS.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Olivier,M. and Cox,D.R.
  TITLE     Unpublished, Olivier, M., Cox, D.R. (2000)
  JOURNAL   Unpublished
COMMENT     
            Contact: Michael Olivier, David R. Cox
            Stanford Human Genome Center
            Stanford University School of Medicine
            4005 Miranda Ave. 2nd Fl., Palo Alto, CA 94025, USA
            Tel: (650) 320-5800
            Fax: (650) 320-5801
            Email: olivier@shgc.stanford.edu
            Primer A: TGTGCAGGACGTCTCATCTC
            Primer B: GTAAGCACAGCCTGAGAGGG
            STS size: 149
            PCR Profile:
                    Initial incubation: 94 degrees C for 90 seconds
                    Denaturation:       94 degrees C for 15 seconds
                    Annealing:          62 degrees C for 23 seconds
                    Polymerization:     72 degrees C for 30 seconds
                    PCR Cycles:         30
                    Thermal Cycler:     Perkin Elmer 9600
            Protocol:
                    Template:       25 ng
                    Primer:         each 1 uM
                    dNTPs:          each 200 uM
                    Taq Polymerase: 0.05 units/ul
                    Total Vol:      10 ul
            
            Buffer:
                    MgCl2:          2.5 mM
                    KCl:            50  mM
                    Tris-HCl:       20  mM
                    pH:             8.3
            
            Prepared with primer pairs derived from M24122 -- Unigene.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /map="3"
                     /clone_lib="Human"
     STS             27..175
     primer_bind     27..46
     primer_bind     complement(156..175)
BASE COUNT           45 a           76 c           64 g           49 t
ORIGIN      
        1 acctcgtgcc cagaagccag gaaggctgtg caggacgtct catctcccat gtgtgatgct
       61 gacaccaagc ggcctggagt cgtgggaagg aggggagcct taccaagact cctgcaaaaa
      121 cccttggacc tctccacgtg gttgccgtct ctggcccctc tcaggctgtg cttacctgtg
      181 acagcagctc tgtccccacc ccatgggcct catgaataaa ggcttcttgc cggc
//