LOCUS       FX174436                 791 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Pandanus boninensis mRNA, contig: Pb_c12686.
ACCESSION   FX174436
VERSION     FX174436.1
DBLINK      BioProject:PRJDA67377
            Sequence Read Archive:DRR001102
            BioSample:SAMD00011606
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Pandanus boninensis
  ORGANISM  Pandanus boninensis
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Pandanales; Pandanaceae;
            Pandanus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011)
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H.
  TITLE     Development of EST-SSR markers from cDNA library of Pandanus
            boninensis Warburg (Pandanaceae)
  JOURNAL   Unpublished
COMMENT     Several-year-old seedlings of Pandanus boninensis naturally grown
            in Bonin Island were used for RNA extraction. About 3 g of inner
            bark was cut and frozen in liquid nitrogen, ground using mortar
            and pestle. Total RNA was extracted by the CTAB method (Le Provost
            et al., 2007), and contaminated DNA was removed by SV total RNA
            isolation system (Promega, Madison, WI). The cDNA library
            construction and next generation sequencing by Roche 454 GS FLX
            sequencer with titanium chemistry were carried out by the Hokkaido
            System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and
            adapter sequences from the cDNA sequences, the Program cross_match
            (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR
            SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..791
                     /db_xref="taxon:1035881"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Pb_c12686"
                     /organism="Pandanus boninensis"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          216 a          188 c          120 g          267 t
ORIGIN      
        1 acaatgtctc cctccacttt ctcaacccat cccataagat ttttgagatc ccctcgtaca
       61 actataactg catcaccttt cataaaatgg cctttctttc tatttgcaaa taaagtagaa
      121 agactatcca tttcaccatt ggcatcatct cccggcttcc ggaatttctc aagctcatca
      181 aacgtaggtt gtatgttttg agaattaatt gatttaatgg acactgtttt atacaaaaac
      241 ccatctttga acatcatatt atctacagtt tcaaaatatt ccccagaatc tctgtccttc
      301 ctgcgttcta cacggatatg cagttccctc gcttcatcaa tgttgaagaa acgaggtggc
      361 ggtacaaaaa tacttctttt ttactaacat ccctaccttc caacttatta gcaagagttt
      421 gtagatcgat tcttgggtat cagtttaacg gttcaccttc tgtcgtacat tgtcaacatc
      481 aacaaccttg gcaagatctc ctttatatat tccaagcttc attcggaccc atgttcccct
      541 agcaagatcg acagttttac tttcaacaga aaggacatct gtcatttctt ttataggtac
      601 cagtagtatt tttgctgagc tgaatatatt ccgcagccct ttgcaagcct ctttgacatg
      661 ggcttctttg tctgcttcaa catatatata atttttaaga tggtccaaag caataactga
      721 tctgatctgg agttcaggcc tatctatgaa tttttgcatg aggcagatag ctgtctctcg
      781 ttcacgacca a
//