LOCUS       FX156427                 357 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Schima mertensiana mRNA, contig: Sm_rep_c10850.
ACCESSION   FX156427
VERSION     FX156427.1
DBLINK      BioProject:PRJDA67331
            Sequence Read Archive:DRR001101
            BioSample:SAMD00003860
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Schima mertensiana
  ORGANISM  Schima mertensiana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; Ericales; Theaceae; Schima.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011)
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H.
  TITLE     Isolation and characterization of EST-SSR markers in Schima
            mertensiana using next-generation pyrosequencing
  JOURNAL   Unpublished
COMMENT     Several-year-old seedlings of Schima mertensiana naturally grown
            in Bonin Island were used for RNA extraction. About 3 g of inner
            bark and current year twigs were cut and frozen in liquid
            nitrogen, ground using mortar and pestle. Total RNA was extracted
            by the CTAB method (Le Provost et al., 2007), and contaminated DNA
            was removed by SV total RNA isolation system (Promega, Madison,
            WI). The cDNA library construction and next generation sequencing
            by Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..357
                     /db_xref="taxon:678541"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Sm_rep_c10850"
                     /organism="Schima mertensiana"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT           82 a           87 c           86 g          102 t
ORIGIN      
        1 cttgatttca cgcagggcag cctcacgagc agcatctcgg acttctggct tctcagttct
       61 tctcttctgt ataacttcca aagtggcacc aactatagac cttgagtagg gcttcttggt
      121 ggctcgtcgc cttttcttca cagcttcagc agaaatatcc ttcttgtgtt gcttcctgta
      181 cattgcagtc caggaaagct ttgacggctt caggcgattg tggaagtagt tcttgcattt
      241 tgagttggca aagaggaaaa cctgagagtc cgagcgaata aatctgatac ccttgccagg
      301 gtatatcttg gcaccactga agcggcaaag ttcagtcttg agaaccatgg cagcagc
//