LOCUS       FX145299                 765 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_c11404.
ACCESSION   FX145299
VERSION     FX145299.1
DBLINK      BioProject:PRJDA67329
            Sequence Read Archive:DRR001057
            BioSample:SAMD00013926
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Elaeocarpus photiniifolius
  ORGANISM  Elaeocarpus photiniifolius
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae;
            Elaeocarpus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011)
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and
            Yoshimaru,H.
  TITLE     Development of EST-SSR for Elaeocarpus photiniifolius
            (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands
  JOURNAL   Unpublished
COMMENT     Several-year-old seedlings of Elaeocarpus photiniifolius naturally
            grown in Bonin Island were used for RNA extraction. About 3 g of
            inner bark, current year twigs and terminal buds were cut and
            frozen in liquid nitrogen, ground using mortar and pestle. Total
            RNA was extracted by the CTAB method (Le Provost et al., 2007),
            and contaminated DNA was removed by SV total RNA isolation system
            (Promega, Madison, WI). The cDNA library construction and next
            generation sequencing by Roche 454 GS FLX sequencer with titanium
            chemistry were carried out by the Hokkaido System Science Co.,
            Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from
            the cDNA sequences, the Program cross_match
            (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR
            SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..765
                     /db_xref="taxon:232873"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Ep_c11404"
                     /note="synonym: Elaeocarpus photiniaefolius"
                     /organism="Elaeocarpus photiniifolius"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          211 a          183 c          134 g          237 t
ORIGIN      
        1 ttctgcatgt ctggatctcc ttcctcttgc aatttgtaac agtgccatat atggattcca
       61 ttctttgctg ctcaaatctg tgtcatggta cttcacatta tatcctgctt tctgaagcaa
      121 ccatgtcact tcgctaacta cttgctctat tggcacatta gttttctcta gcttaagata
      181 tgccttgatt gcttcaccag ccctttcaga cacatcgagc attagcaatg tagacatcaa
      241 acgattacgt tcaccttttt tcattcttct tttgcccttc tcattaatga atgtctcaat
      301 ctcagttgca agcacttgac ggacagtcat gcttctatct aatttagaac catcttcgtc
      361 aggagataca aatggagtgt tcattttttc ccctgccacc atcaacaaag cgacaaactt
      421 aacgagtttc ccttcctttg cataccggta catttccatc ccaatccctt gtgtgttcaa
      481 cgcaagcaag ctgatacagt ccattggtat tttccaatct ggcaggcaca atgtgataat
      541 caattggaaa atagattggt ctggagtcca ataaaataat gagatccaat tcgatcttaa
      601 tgtgtaaatt gctacatcaa ctggtagcaa cccttgaaaa aactcatcat cagaagggct
      661 tcgaacatca gatcgagctc catgatgaag gagtacctcg ataaagtcag tgaagcaaca
      721 aactagctgc ccggtgtaat ggatgaacac cggcgagcta gtgtg
//