LOCUS       FX126946                 804 bp    mRNA    linear   TSA 17-MAY-2014
DEFINITION  TSA: Calophyllum inophyllum mRNA, contig: Ci_c10850.
ACCESSION   FX126946
VERSION     FX126946.1
DBLINK      BioProject:PRJDA67327
            Sequence Read Archive:DRR001052
            BioSample:SAMD00016598
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Calophyllum inophyllum
  ORGANISM  Calophyllum inophyllum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae;
            Calophylleae; Calophyllum.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011)
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H.
  TITLE     Microsatellite markers derived from Calophyllum inophyllum
            (Clusiaceae) expressed sequence tags
  JOURNAL   Am. J. Bot. 99 (1), e28-e32 (2012)
  REMARK    Publication Status: Online-Only
            DOI:10.3732/ajb.1100299
COMMENT     Several-year-old seedlings naturally grown in Bonin Island were
            used for RNA extraction. About 3 g of inner bark, current year
            twigs and terminal buds were cut and frozen in liquid nitrogen,
            ground using mortar and pestle. Total RNA was extracted by the
            CTAB method (Le Provost et al., 2007), and contaminated DNA was
            removed by SV total RNA isolation system (Promega, Madison, WI).
            The cDNA library construction and next generation sequencing by
            Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..804
                     /db_xref="taxon:158927"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Ci_c10850"
                     /organism="Calophyllum inophyllum"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          231 a          209 c          150 g          213 t
ORIGIN      
        1 gccccagcac agttttgccc actagattgc aggacggccc tgacagcaac ttgtgcaaca
       61 tgaggcacat ccacatcttc acacacaata aatgcatctt ttccaccaag ctcaagagta
      121 acaggtgtga ggttgtagga agcgtttttc attatcactt tgcccacagc aggtgatcca
      181 acaaatatga ctttawcaac tgaagaaacc aatgcttctc ctgtttcagc aaaccctgtt
      241 attacgtcaa ccaaattttc aggagcaccc acagcagcaa gagcttgctg aattatgcgg
      301 aagtagaagc atcctgacca acttgctatt ttactgaaac cttaatacac aacactgttt
      361 cctgaaaaag actgctgcca gcattggatt aaagatattg tggaatgggt aattccagga
      421 aacaattgca ccaataacac caagggggtg aaactccacc ttagacttct tgtgaagcat
      481 tcgacctccc agaagaacgg tattcaggct ttagccactg ctcaccctcc gaaagaagcc
      541 aagttatctt ttcacatgtg gtcattattt cccctaatga ggcatctacc attgtctttc
      601 cagtatcacg tgaagagact tggcagataa gctcttggtg ctcaataata tacttcaaaa
      661 gtatcctcaa aaaactgcct cctctgcttg aagctgctct tcgcccaagc ctttttgagc
      721 cttccttgcc tgtgctacac gttctttgac ctcatcagct gacaaagcag ggaaatatcc
      781 caagtatttc atggtcgccg gctc
//