LOCUS FX126946 804 bp mRNA linear TSA 17-MAY-2014 DEFINITION TSA: Calophyllum inophyllum mRNA, contig: Ci_c10850. ACCESSION FX126946 VERSION FX126946.1 DBLINK BioProject:PRJDA67327 Sequence Read Archive:DRR001052 BioSample:SAMD00016598 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Calophyllum inophyllum ORGANISM Calophyllum inophyllum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae; Calophylleae; Calophyllum. REFERENCE 1 (bases 1 to 804) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (26-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H. TITLE Microsatellite markers derived from Calophyllum inophyllum (Clusiaceae) expressed sequence tags JOURNAL Am. J. Bot. 99, e28-e32 (2012) REMARK Publication Status: Online-Only DOI:10.3732/ajb.1100299 COMMENT Several-year-old seedlings naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/ phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..804 /db_xref="taxon:158927" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ci_c10850" /organism="Calophyllum inophyllum" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 231 a 209 c 150 g 213 t ORIGIN 1 gccccagcac agttttgccc actagattgc aggacggccc tgacagcaac ttgtgcaaca 61 tgaggcacat ccacatcttc acacacaata aatgcatctt ttccaccaag ctcaagagta 121 acaggtgtga ggttgtagga agcgtttttc attatcactt tgcccacagc aggtgatcca 181 acaaatatga ctttawcaac tgaagaaacc aatgcttctc ctgtttcagc aaaccctgtt 241 attacgtcaa ccaaattttc aggagcaccc acagcagcaa gagcttgctg aattatgcgg 301 aagtagaagc atcctgacca acttgctatt ttactgaaac cttaatacac aacactgttt 361 cctgaaaaag actgctgcca gcattggatt aaagatattg tggaatgggt aattccagga 421 aacaattgca ccaataacac caagggggtg aaactccacc ttagacttct tgtgaagcat 481 tcgacctccc agaagaacgg tattcaggct ttagccactg ctcaccctcc gaaagaagcc 541 aagttatctt ttcacatgtg gtcattattt cccctaatga ggcatctacc attgtctttc 601 cagtatcacg tgaagagact tggcagataa gctcttggtg ctcaataata tacttcaaaa 661 gtatcctcaa aaaactgcct cctctgcttg aagctgctct tcgcccaagc ctttttgagc 721 cttccttgcc tgtgctacac gttctttgac ctcatcagct gacaaagcag ggaaatatcc 781 caagtatttc atggtcgccg gctc //