LOCUS       FX126348                 778 bp    mRNA    linear   TSA 17-MAY-2014
DEFINITION  TSA: Calophyllum inophyllum mRNA, contig: Ci_c10252.
ACCESSION   FX126348
VERSION     FX126348.1
DBLINK      BioProject:PRJDA67327
            Sequence Read Archive:DRR001052
            BioSample:SAMD00016598
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Calophyllum inophyllum
  ORGANISM  Calophyllum inophyllum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae;
            Calophylleae; Calophyllum.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011)
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H.
  TITLE     Microsatellite markers derived from Calophyllum inophyllum
            (Clusiaceae) expressed sequence tags
  JOURNAL   Am. J. Bot. 99 (1), e28-e32 (2012)
  REMARK    Publication Status: Online-Only
            DOI:10.3732/ajb.1100299
COMMENT     Several-year-old seedlings naturally grown in Bonin Island were
            used for RNA extraction. About 3 g of inner bark, current year
            twigs and terminal buds were cut and frozen in liquid nitrogen,
            ground using mortar and pestle. Total RNA was extracted by the
            CTAB method (Le Provost et al., 2007), and contaminated DNA was
            removed by SV total RNA isolation system (Promega, Madison, WI).
            The cDNA library construction and next generation sequencing by
            Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..778
                     /db_xref="taxon:158927"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Ci_c10252"
                     /organism="Calophyllum inophyllum"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          219 a          181 c          195 g          183 t
ORIGIN      
        1 cttcacttca aacgagggat actagcatgt gaagttacag atctgaaact ctaaacgaca
       61 ccgttttgct gcggttagtt agaatcgtta aaccagaagt ggagctaagc taggtttgtc
      121 agtgatctaa acgcgggagt ggtattattg gaattttcta gagggaaaaa tgaaccggag
      181 ttttagggca caagagtcgc cacagttgca aatgcaagcg gtggttacta agcagaggca
      241 acagctaagg gcttcaatgg ttaagaatag taactgtaag gagaaggaag aggagcagct
      301 ggcgttgttc ctggagatga agaagtctga aaaggaccac aataaccttc tcctcctcca
      361 tagccccgaa gagttcgacg atcctccggg gtcgaagccg gggacttcgc ctatgtttaa
      421 tatctcatcc tctgcgacgc cggcgcggaa agttggtgcc gatgatattc tcaactcgga
      481 aaatgataag aatgactatg actggcttct gacaccccct ggtactcctc tgtttccttc
      541 tttggagatg gaatctcaga agactatcat gaatcagttg ggaactacaa aggctagtgc
      601 accagctccc aagtctagac tggcaaatcc tcagtcggag ccagctggaa ggagcaactt
      661 cgtatctaaa caatctttgt ccgcccccag tttgagctcc tctggaatgc gaaggccctc
      721 atcatcagga ggaccaggat caagacctgc aacaccgact tcacgtccta cattgcca
//