LOCUS FJ430073 7028 bp DNA linear PLN 23-DEC-2010 DEFINITION Emericella nidulans strain FGCS 4 biotin biosynthesis gene cluster, complete sequence. ACCESSION FJ430073 VERSION FJ430073.1 KEYWORDS . SOURCE Aspergillus nidulans ORGANISM Aspergillus nidulans Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Nidulantes. REFERENCE 1 (bases 1 to 7028) AUTHORS Magliano,P., Flipphi,M., Sanglard,D. and Poirier,Y. TITLE Characterization of the Aspergillus nidulans biotin biosynthetic gene cluster and use of the bioDA gene as a new transformation marker JOURNAL Fungal Genet. Biol. 48 (2), 208-215 (2011) PUBMED 20713166 REFERENCE 2 (bases 1 to 7028) AUTHORS Flipphi,M. TITLE Direct Submission JOURNAL Submitted (31-OCT-2008) Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Cientificas, Apartado de Correos 73, Burjassot, Valencia 46100, Spain FEATURES Location/Qualifiers source 1..7028 /organism="Aspergillus nidulans" /mol_type="genomic DNA" /strain="FGCS 4" /db_xref="taxon:162425" /note="teleomorph: Emericella nidulans" misc_feature 1..7028 /note="biotin biosynthesis gene cluster" gene complement(<108..>1462) /gene="biF" mRNA complement(join(<108..434,502..644,694..>1462)) /gene="biF" /product="8-amino-7-oxononanoate synthase" CDS complement(join(108..434,502..644,694..1462)) /gene="biF" /EC_number="2.3.1.47" /function="catalyzes the formation of 8-amino-7-oxononanoate from 6-carboxyhexanoyl-CoA and L-alanine" /note="BiF; 7-keto-8-aminopelargonate synthetase; the preantepenultimate step of biotin biosynthesis; absent in Saccharomyces cerevisiae" /codon_start=1 /product="8-amino-7-oxononanoate synthase" /protein_id="ACR44942.1" /translation="MGDSPKCLRDSLREALRRREDKLCRRKLTILPSSSVDFSSNDFL SLSTSPAYRARFLDILQQAPPLHPFASGGSRLLDGNSAYAEELENFIAAFHNAPSGLL FNSGYDANVGVFSSIPQPGDLIVYDELIHASAHEGMRLSRAGKRIKFPHSSPDGLRAV LQAEITADPRLLQGRRNVFIAFESVYSMDGDVAPIREFVEIVDQLLPYGNGYFLVDEA HATGVFGPRGSGVVQELGLEDRMFVRVHTFGKALASHGAIVLCCADTRDYLINYARSL IYTTALGFPFLASIRAAYELLVEGKTEQLQHKLGQLIAHFRTGLDNLNHKDSSTFEVE HFTNSPIFSLRSSVPRVLASVCQEQGYTVRAIMPPTVPAGKERVRVCLHAGNTVEEVD GLLETIATWLQRMEKQKARL" gene <2171..>4764 /gene="biDA" mRNA join(<2171..2358,2409..2502,2556..3889,3942..4516, 4592..>4764) /gene="biDA" /product="bifunctional dethiobiotin synthetase/adenosylmethionine-8-amino-7-oxononanoate aminotransferase" CDS join(2171..2358,2409..2502,2556..3889,3942..4516, 4592..4764) /gene="biDA" /EC_number="6.3.3.3" /EC_number="2.6.1.62" /note="BiDA; penultimate step of biotin biosynthesis; antepenultimate step of biotin biosynthesis" /codon_start=1 /product="bifunctional dethiobiotin synthetase/adenosylmethionine-8-amino-7-oxononanoate aminotransferase" /protein_id="ACR44943.1" /translation="MAPVGAALWRSLRAHQVYGANTDVGKTIVSTFLCNAVNRLKNQG KSAFLKPVSTGPLDEADDRHLQRHAPNTLTKCLYQFDEPVSPHIAAKTFAIPRDDEIL SSVHRTLSDWANDGVGFALVETAGGVHSPGPNGNSQADLYRPLRLPIILVADSRLGGI SSSISAYESLLLRGYDVHSVLLFKDDYYQNHEYLGNYFRGKSIPLVPVPAPPRRPQEQ DPDSRARDLEALDKYYSSVTKSTDVVSLLDELVLKNKQRVEYLDEMASRAQKTIWYPF TQHHGMAAKDITPIDSAYDDFFQTYVTADRSAQQGRLQATFDGSASWWTQGLGHGNPG LALSAAYAAGRYGHVMFPGNIHEPALALAESLLKTVDNPRLQKVFYTDNGSTGMEVAL KMGLRAACDRYGWDASKEQINILGLKGSYHGDTIGVMDCSEPSTYNQRVEWYRGRGHW FDFPLVKMSQGVWQVEVPATLQASLGGNQQFSSLDAVFDVESRVRSDAGQRYRKYILE TIERLVTQEGKKFGALIMEPIILGAGGMLFCDPLFQRCLADVVRGNPQLFNRGRLTEP QPQTDLSWSGLPVIFDEVFTGLYRLGRKSSASFLGVNPDIAVNAKLLTGGLVPLCTTL ASNEIFNAFTSPEKRDALLHGHSYTAHAVGCQVALDSLRTMNNMDEDGSWNDFKNDWK QPHAGDTARVWSVWSHKLLHNLSHAESVDGVFAIGSVLSISLKDAEGAGYTSTAAKGL QTRLAAGGPQFNVHSRVLGNVLYLMSSVTSKQETLRTIEGILREALL" gene <5678..>6956 /gene="biB" mRNA join(<5678..5887,5985..>6956) /gene="biB" /product="biotin synthase" CDS join(5678..5887,5985..6956) /gene="biB" /EC_number="2.8.1.6" /function="catalyzes the conversion of dethiobiotin to biotin" /note="BiB; dethiobiotin:sulfur sulfurtransferase; ultimate step of the biotin biosynthesis" /codon_start=1 /product="biotin synthase" /protein_id="ACR44944.1" /translation="MSVSFTRSFPRAFIRSYGTVQSSPTAASFASRIPPALQEAVAAT APRTNWTRDEVQQIYETPLNQLTYAAAAVHRRFHDPSAIQMCTLMNIKTGGCSEDCSY CAQSSRYSTGLKATKMSPVDDVLEKARIAKANGSTRFCMGAAWRDMRGRKTSLKNVKQ MVSGVREMGMEVCVTLGMIDADQAKELKDAGLTAYNHNLDTSREFYPTIITTRSYDER LKTLSHVRDAGINVCSGGILGLGEADSDRIGLIHTVSSLPSHPESFPVNALVPIKGTP LGDRKMISFDKLLRTVATARIVLPATIVRLAAGRISLTEEQQVACFMAGANAVFTGEK MLTTDCNGWDEDRAMFDRWGFYPMRSFEKETNAATPQQHVDSVAHESEKNPAAPAAEA L" BASE COUNT 1633 a 1958 c 1806 g 1631 t ORIGIN 1 cagtgccgga atcatggctt catgatttgc ctgacacatg taccgtacat gaaggacttc 61 tgcgtcaatg gggatgtgat ttgtgggctc tgggtctgat gataatgcta taaccgagcc 121 ttttgctttt ccatccgctg taaccacgta gcaatggtct caaggaggcc gtcgacctcc 181 tccaccgtgt ttcccgcgtg caagcatacg cgtacacgtt ctttccctgc tggaacagtt 241 ggcggcatga ttgcacggac ggtatatccc tgctcctggc atacgctggc gagcacacgg 301 ggcacactgc tgcgaaggga aaatatgggt gagttcgtaa aatgttcaac ttcaaaggtc 361 gacgagtcct tgtggttgag attgtccaac cccgtccgga agtgagcaat cagttgtccg 421 agtttgtgct ggagctggat atttgttagc tttcccatag gcctgtaatg aaacgttgct 481 tcctcaagcg aaggaccgta cctgctcagt ttttccctca acgagtagtt cataggcagc 541 acgtattgaa gcaagaaagg gaaatcccag ggcagtggta tagatcaggc tgcgagcgta 601 attgatcagg tagtctctag tatccgcaca acacaatacg attgctagca ggacagtcaa 661 tatcgacacc caaagacaac gcgcgagact tacctccatg actagcgagt gctttaccga 721 acgtatgtac ccggacgaac atacgatcct ccagtcccaa ctcttggact accccagaac 781 cgcgtggtcc aaagacgccg gtcgcatgcg cctcatccac aaggaagtat ccgttaccgt 841 aagggagaag ctggtccacg atctctacga actcacgaat aggcgcaaca tcgccgtcca 901 tactgtagac ggattcaaag gcgatgaaaa cgttccggcg gccctgaaga agtcggggat 961 ctgcagttat ttctgcttgc agtactgctc tcaggccatc gggagagcta tgagggaact 1021 tgatacgctt ccctgctcgt gagagccgca tgccctcatg cgcgctggca tggatgagtt 1081 catcgtatac tatcagatct ccgggttgcg ggatactcga gaaaacgcca acattggcat 1141 catatccgga attgaacagt aggccactcg gggcattgtg gaacgcggca atgaagttct 1201 ccagctcttc ggcataggct gaattgccgt ctaggaggcg ggaccctccg ctggcaaacg 1261 ggtgcaacgg cggcgcttgt tgcaaaatat ccaaaaaccg cgctcgatag gctggcgatg 1321 tggatagtga caaaaagtcg tttgatgaga aatcaaccga agacgaaggt agaatcgtca 1381 gttttcgacg gcacagcttg tcttcccttc gacgtaacgc ctcgcgcagt gagtcacgaa 1441 ggcattttgg agaatcgccc atctggacga ctgtggttcc aaggccaacg cttgccaata 1501 ggcaattcag atagctggag acaagattca agtaaccgta aattcaaagg aggatctatt 1561 ttttctctgg tgagattttg ccagtattaa tgctccagct caaatcacgc tctccaatct 1621 ccacgagtct agaacgtctt gaagaccctg ctgtatggac ctgagacttg gggtagagcg 1681 atcactgatg aagtagtgcc tgagtctcac cacattaaca catcatacgg agtagcacaa 1741 ttcaattcgg cgggacttat ttcatctgtt gggatcaacc ggtgggggaa tggatccgac 1801 gaacgtggct gggccgctgc ccggttgtgc cgctgcagtg actcggtgag caacgcagtt 1861 ccctaggcaa gacaaaaggc ttaactcggg ggcctcggcc tggttcctgt ctcactgtgc 1921 ctgttcctgc tagaacgact atgaacgcct aatgcaaagg gctcgagagt cgaaccacga 1981 tcgggtttct ttccgcggta aagcgctgca gccttctgca accgaatctg tcggtcatca 2041 ttcctgttcc ggtcgcagac gggtgccgaa aactgcttct aaagttcagt tggtgtttcg 2101 gctctgtgat gcgttagcct ttcacattag cgactggttt ctgctgtttt gaaagtctag 2161 attcaccatc atggcacctg ttggtgccgc gctctggcgc tccctccgcg cccaccaagt 2221 atacggggcg aacactgacg tcggcaagac aattgtatca acatttcttt gcaatgctgt 2281 taatcgtctg aaaaatcaag gaaagtcggc gtttttgaag ccggtctcga cggggccatt 2341 ggatgaagcg gatgacaggt aaggccggga tgtcggcgag agagtgttac cgactcacgc 2401 gaacacaggc acctgcaacg ccatgctccc aacactttga caaaatgcct ctatcaattt 2461 gatgaacccg tcagcccaca tatcgcagct aagacgttcg ctgtcagaaa cccaaatcta 2521 cttcgtccaa cataagcaca cactaacctc gacagattcc tcgagatgac gagatccttt 2581 cctctgtcca caggaccctt tccgactggg ccaacgacgg agtcgggttc gctctggtag 2641 aaacggccgg tggtgttcac tcgccgggcc ccaatgggaa ctcccaggcc gacctctacc 2701 gacctcttcg gttgcccatc atcttggtcg ctgactcccg tcttggggga atctcatcct 2761 ctatatccgc ctacgagtct ctcttgctcc gcggatacga tgtccactcc gtccttctat 2821 ttaaggatga ctactatcag aatcatgagt acttggggaa ctacttccgg ggcaagagca 2881 taccgcttgt cccggtgcct gcaccgcctc ggcgtccgca agagcaagac cccgactcgc 2941 gagctcgcga tctggaagcg ttggacaagt attacagctc agttacaaaa agcacggatg 3001 tggtctcgct cctagatgag cttgttttga aaaacaagca gcgtgtcgaa tacctcgacg 3061 agatggcaag ccgagcacag aagacaatct ggtatccatt tacgcagcac cacggcatgg 3121 cggcaaagga tatcactccc atcgattccg catacgatga tttcttccag acttacgtga 3181 ctgcggatcg ttcggcacaa caaggcagac tccaagccac gttcgacgga tccgcatcgt 3241 ggtggacaca gggactcggt cacggaaatc ccggccttgc tctatctgct gcgtatgccg 3301 caggacgtta cgggcatgta atgttccccg ggaacattca tgagccggct ttggcgttgg 3361 cggaatcgct cttgaagact gtggataacc ctcgcctaca gaaggtcttc tatacagaca 3421 atggcagcac aggtatggag gtagctctca agatgggcct ccgcgctgcg tgcgaccggt 3481 atggctggga tgcaagcaaa gaacagatca acattctcgg cctcaaaggg agctaccatg 3541 gcgacacgat cggggttatg gactgctcag agccgtcgac ctacaatcaa agagttgagt 3601 ggtacagagg ccggggtcac tggtttgatt tccctctggt aaagatgtcc caaggggtct 3661 ggcaggtcga ggtccccgca acactgcaag cgtcgcttgg cgggaaccag cagttctcat 3721 cactggatgc agtatttgat gtagaaagca gggtccgctc ggatgctgga cagcgctaca 3781 ggaagtatat actagagacc attgagcgcc tggttactca agaagggaag aagtttggcg 3841 ccctaattat ggaacctatc attctaggag ccggcggaat gctcttctgg tcagtataga 3901 tcctttttta caggaaagtt actgacggac gaccgttata gtgatcccct gttccagcga 3961 tgcctcgccg atgtcgtccg cggtaacccc cagctcttca accgaggccg gttgacagaa 4021 ccgcagccgc agaccgacct ctcgtggtcc ggtctccccg tcatctttga cgaggttttc 4081 acgggcctct accgtcttgg ccgtaaatcc tctgcgtcat ttctaggtgt caacccagac 4141 atcgctgtca acgcaaagct ccttaccgga ggcctggtgc cgctctgcac taccctagcc 4201 agcaacgaaa tcttcaacgc gtttacaagc ccggagaagc gagatgcgct cttgcatggg 4261 cacagctaca ccgcgcatgc ggtcgggtgc caagtagccc ttgattccct tcggacaatg 4321 aacaatatgg acgaggacgg atcctggaac gacttcaaga acgactggaa acaaccccat 4381 gccggcgaca ccgcccgggt ctggagcgtc tggtcccaca agctgctcca caacctttcc 4441 cacgccgaat ccgtcgatgg cgtcttcgct attggtagcg ttctcagcat ctctctgaaa 4501 gatgcagagg gtgcaggtga gttcccgact ctctctacaa caattctctc gccgattcta 4561 cccgctaaca tgtgacacct ccactcatta ggctatacat ccactgcggc taagggcctt 4621 cagacaagat tagcggctgg tgggccgcag tttaacgtcc actctcgggt cctcgggaat 4681 gtgctttact tgatgtcaag cgtgacgtcc aagcaggaaa cactgcggac aattgagggc 4741 atcctgcgcg aggcgctgct gtaacttaca acctaactag catgtacatg actaagctgt 4801 actctctgtg taaacaagcg tcgtctcacc ggcaaccgta agaaaaaagt gtggacaccg 4861 gaccgccgga taggctggtg cgatcgtttg aacccttgta gaatccagct caccgagtca 4921 ccgggtctcg atatggagcc aagatttact attgatgccc gctctcgctg gctgagtctg 4981 gatcttacag agacacacta gctcaggttc actgagctcc ggcctcgcag gcccggcgac 5041 aatctacccc gctattcgcc aggagcaccg acttccgagg ttattctaaa ttggctctta 5101 gtgagtctcc cgcagatctg cagtttagca ccttgctttt tgctgtcgaa tcagacccaa 5161 ggggataaga tcaggcactg ggctcgctga ttggtcggac ccgttctcca tctggaaata 5221 cgcgatccac ttccgtcagt cgttcactcg aaatacgtaa gacctcagga cttgcgttga 5281 accagcgttg gggtcaacac tcctgcaaag gaagagtccg tttgcagcga tagcaagcga 5341 atcaagctaa tcaagccaat caagcgatag gaagcgatag caaataattg tagttgaggt 5401 caggcaattt gctcagaatt attggaccaa tttggaccaa gttgggccaa tttaggccca 5461 taataaatta caaagttaca agagttcact ctgtagcggt tttccaccga catttcactc 5521 ctctccaggc caatgacgag ttacccttct tcaggttcct attgtggccc atgctcctct 5581 cacctgcatt ttcccctccc gccgcatact tttgatcacc cctcgctgtt tcccgcagtg 5641 tcaccttgat acactcctcc aaccacccgt catcactatg tctgtctcgt ttacgcggtc 5701 ctttcctagg gccttcataa ggtcatatgg caccgtccag tcgtcgccca cggccgcttc 5761 ctttgcgagc agaatccccc cggctctcca ggaggctgtt gcagccactg ccccgcgcac 5821 caattggact cgcgatgaag tccagcagat ttacgagacc ccgttgaatc aattaaccta 5881 cgctgctgta tgtttccgat ttgaccgctg cttataaatt attctgtaac gcggatattt 5941 gaatgaattg ttcgttggac cgaatgttga ctcatgctga ataggccgct gtccaccgcc 6001 gctttcatga cccgtccgca atccaaatgt gcacgttgat gaacatcaag accggtggat 6061 gcagtgaaga ttgctcctac tgtgcacagt cttctcggta cagcactggc ctcaaggcca 6121 ccaaaatgag ccccgtcgac gacgtcctcg agaaggcgag gattgccaaa gcgaacggga 6181 gcacgcgttt ctgtatggga gcggcgtggc gtgatatgcg gggtcgtaag acgagtttga 6241 agaatgtcaa gcagatggta tctggcgttc gggaaatggg aatggaagtc tgcgtcacac 6301 taggcatgat tgatgctgat caggctaagg agctgaaaga tgccggcctg acagcctaca 6361 accacaacct cgatacttcg cgcgaattct accccacaat catcacaacc cgatcgtacg 6421 acgaacgact aaagaccttg tctcatgtcc gtgatgcggg cattaacgtc tgctctggtg 6481 gtattctagg tcttggtgag gctgactctg accgcatcgg cctcatccac acggtttcgt 6541 cacttccctc gcacccggag tcttttcccg tcaacgcctt ggttccaatc aagggtaccc 6601 cgttgggtga caggaaaatg atctctttcg ataagctcct ccgcactgtc gcgactgcac 6661 ggatcgtcct tcccgcaacc atcgtccgcc tcgccgccgg ccgcatttct ctcacagagg 6721 agcagcaggt tgcctgcttc atggctggtg caaacgctgt cttcactgga gagaagatgt 6781 tgactactga ctgcaacggc tgggacgagg accgcgccat gtttgaccga tggggcttct 6841 accccatgcg cagctttgag aaagagacta acgctgccac cccccagcag catgttgact 6901 ctgttgctca cgagtccgag aagaaccccg ctgcgccggc cgcagaagcc ctatgatagg 6961 gctctaaaac taccccaccc ccccagcctg atacgctttt ctccctgtcc gtgattggta 7021 gggaagcg //