LOCUS       EU165356                1490 bp    mRNA    linear   PLN 27-FEB-2008
DEFINITION  Nicotiana benthamiana putrescine N-methyltransferase (PMT) mRNA,
            complete cds.
ACCESSION   EU165356
VERSION     EU165356.1
KEYWORDS    .
SOURCE      Nicotiana benthamiana
  ORGANISM  Nicotiana benthamiana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; lamiids; Solanales; Solanaceae;
            Nicotianoideae; Nicotianeae; Nicotiana.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Liu,E. and Page,J.E.
  TITLE     Optimized cDNA libraries for virus-induced gene silencing (VIGS)
            using tobacco rattle virus
  JOURNAL   Plant Methods 4, 5 (2008)
   PUBMED   18211705
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Liu,E. and Page,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2007) NRC Plant Biotechnology Institute, 110
            Gymnasium Place, Saskatoon, SK S7N 0W9, Canada
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Nicotiana benthamiana"
                     /mol_type="mRNA"
                     /db_xref="taxon:4100"
     gene            1..1490
                     /gene="PMT"
     CDS             71..1237
                     /gene="PMT"
                     /EC_number="2.1.1.53"
                     /note="S-adenosyl methionine-dependent methyltransferase
                     enzyme"
                     /codon_start=1
                     /product="putrescine N-methyltransferase"
                     /protein_id="ABY25273.1"
                     /translation="MEVISTNKNGSNIFKNGAIPMNGYKNGTSKHQNGHQNGTSEHRT
                     GHQNGISEHQNDHQNGTSEHQNDHHNGRSEQQNGTISHDNGNELQLLGSSNSIKPGWF
                     SEFSALWPGEAFSLKIEKLLFKGKSHYQDVMLFESATYGKVLTLDGAIQHTENGGFPY
                     TEMIVHLPLSSIPNPKKVLIIGGGIGFTLFEMLRYPSIEKIDIVEIDDVVVDVSRKFF
                     PYLAANFNDPRVTLVLGDGAAFVKAAQAGYYDAIIVDSSDPIGPAKDLFERPFFEAVA
                     KALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGYMLC
                     STEGPQVDFKNPVNPIDKETTQVKSKLAPLKFYNSDIHKAAFILPSFARSMIES"
BASE COUNT          464 a          317 c          286 g          423 t
ORIGIN      
        1 acagcaagct ttcagaaaat acaaaccata atactttctc ttctccaatt tctttagttt
       61 aattttgaaa atggaagtca tatctaccaa caaaaatggc tctaatatct tcaagaatgg
      121 tgccattccc atgaacggct acaagaatgg cacttccaaa caccaaaacg gccaccagaa
      181 tggcacttcc gaacatcgga ctggccacca gaatggaatt tccgaacacc aaaacgacca
      241 ccagaatggc acttccgaac atcaaaacga ccatcataat gggagatccg aacaacagaa
      301 cgggacaatc agccatgaca atggcaacga gctacagcta ctgggaagct ccaactctat
      361 taagcctggt tggttttcag agtttagcgc attatggcca ggtgaagcat tctcacttaa
      421 gattgagaag ttactattca aggggaaatc tcactaccaa gatgtcatgc tctttgagtc
      481 agcaacatat gggaaggttc tgactttgga tggagcaatt caacacacag agaatggtgg
      541 atttccctac acagaaatga ttgttcatct cccactcagt tccatcccaa acccaaaaaa
      601 agttttgatc atcggcggag gaattggttt tacattattc gaaatgcttc gctatccttc
      661 aatcgaaaaa attgacattg ttgagatcga tgatgtggta gttgatgtgt cgagaaaatt
      721 ttttccttat ctcgcagcta attttaacga tcctcgtgta accctcgtcc tcggagatgg
      781 ggctgcattt gtaaaggctg cgcaagcagg atactatgat gctattatag tagactcttc
      841 tgatcccatt ggtccagcaa aagacttgtt tgagaggcca ttctttgagg cggtagcgaa
      901 agccctaaga ccaggaggag ttgtatgcac acaggctgaa agcatttggc ttcatatgca
      961 tattattaag caaatcattg ctaattgtcg tcaagtcttt aagggctctg tcaactacgc
     1021 ttggactact gttccaactt atccaaccgg tgtgatcggt tatatgcttt gctctactga
     1081 aggaccacaa gttgacttca agaatccagt aaatccaatt gacaaagaga caactcaagt
     1141 caaatccaaa ttagcacctc tcaagttcta caactctgat attcacaaag cagcattcat
     1201 tttgccatct ttcgccagaa gtatgatcga gtcttaatca agtgaacaat gaacactggt
     1261 ggtacaatca ttgggaccaa gatcgagtct ttatcaagtg aataaataaa agtgaaatgc
     1321 aacgcattgt atgaatccag tagtaattat cataattcgg attcaccaat tagtgtagga
     1381 ttcaccaatt agtgtaaatt ctttctgtgg tgtttggttt tttcatataa attttcttgc
     1441 tgttgttttg atatgacgtt tcaactcaat ccacgcaaat catttcatta
//