LOCUS       EF802414                 134 bp    DNA     linear   GSS 12-JUL-2007
DEFINITION  Mus musculus C57BL/6N IST11178D6HMF1 gene trap embryonic stem cell
            insertion site sequence tag (IST), genomic survey sequence.
ACCESSION   EF802414
VERSION     EF802414.1
KEYWORDS    GSS.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 134)
  AUTHORS   Hansen,G.M., Markesich,D., Zhu,Q., Richter,L.J., Abuin,A.,
            Sands,A., Finnell,R.H. and Zambrowicz,B.P.
  TITLE     Large-Scale Gene Trapping in C57BL/6N Mouse Embryonic Stem Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 134)
  AUTHORS   Finnell,R.H. and McLeod,M.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2007) TIGM, 2121 W. Holcombe Blvd, Houston, TX
            77030, USA
COMMENT     Contact: Richard H. Finnell at Texas Institute for Genomic Medicine
            (TIGM)  Phone: 713-677-7429  Fax: 713-677-7790  Email:
            ncbigss@tigm.org  Website: www.tigm.org.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Mus musculus"
                     /mol_type="genomic DNA"
                     /strain="C57BL/6N"
                     /db_xref="taxon:10090"
                     /clone="IST11178D6HMF1"
                     /sex="male"
                     /cell_line="Lex3.13 ES transgenic cell line harboring
                     insertion mutation"
                     /cell_type="embryonic stem cell"
                     /transgenic
                     /note="strain origin confirmed on the basis of 110 genetic
                     markers distributed across 19 autosomes and the X
                     chromosome (Charles River Laboratories); sequence tags are
                     derived from genomic sequence by inverse PCR (IPCR) and
                     represent the gene-trap vector insertion site; sequence
                     reads represent either the upstream (F) or downstream (R)
                     vector-genomic junction, amplified from circularized
                     genomic DNA"
     source          1..30
                     /organism="Gene trapping vector VICTR76"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:447635"
     repeat_region   <1..30
                     /rpt_type=long_terminal_repeat
BASE COUNT           38 a           28 c           24 g           43 t
ORIGIN      
        1 gattgactac ccgtcancgg gggtctttca gcatgttgct gcaaataaca agactgcatg
       61 cttttttatg gctgaatata ggccactgac tgtatacaca acttttttta acccatcaat
      121 gaatacttaa gtta
//